##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935732.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	944178
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08922787864153	33.0	31.0	34.0	30.0	34.0
2	32.09885847795649	33.0	31.0	34.0	30.0	34.0
3	32.08317499454552	33.0	31.0	34.0	30.0	34.0
4	35.7390873331088	37.0	35.0	37.0	33.0	37.0
5	34.82661849778326	37.0	35.0	37.0	33.0	37.0
6	35.32015255597991	37.0	35.0	37.0	33.0	37.0
7	35.838616235497966	37.0	35.0	37.0	35.0	37.0
8	35.87875908991737	37.0	35.0	37.0	35.0	37.0
9	37.64230473491227	39.0	38.0	39.0	35.0	39.0
10	37.30728527883514	39.0	37.0	39.0	34.0	39.0
11	37.3267921938448	39.0	37.0	39.0	34.0	39.0
12	37.28538580648988	39.0	37.0	39.0	34.0	39.0
13	37.28581051454281	39.0	37.0	39.0	34.0	39.0
14	38.48101840966428	40.0	38.0	41.0	34.0	41.0
15	38.576648682769566	40.0	38.0	41.0	34.0	41.0
16	38.546265640588956	40.0	38.0	41.0	34.0	41.0
17	38.47236326201204	40.0	38.0	41.0	34.0	41.0
18	38.377743391606245	40.0	38.0	41.0	34.0	41.0
19	38.360827089807216	40.0	37.0	41.0	34.0	41.0
20	38.325505360218095	40.0	37.0	41.0	34.0	41.0
21	38.24027672748147	40.0	37.0	41.0	34.0	41.0
22	38.234541580083416	40.0	37.0	41.0	34.0	41.0
23	38.27085888465946	40.0	37.0	41.0	34.0	41.0
24	38.2791782905342	40.0	37.0	41.0	34.0	41.0
25	38.0479930691035	40.0	37.0	41.0	34.0	41.0
26	38.13288172357331	40.0	37.0	41.0	34.0	41.0
27	38.076207028759406	40.0	37.0	41.0	34.0	41.0
28	37.68843374872111	40.0	36.0	41.0	33.0	41.0
29	37.39693256991796	40.0	36.0	41.0	32.0	41.0
30	37.31239130757124	40.0	36.0	41.0	32.0	41.0
31	37.343788988940645	40.0	36.0	41.0	32.0	41.0
32	37.36081120297232	40.0	36.0	41.0	32.0	41.0
33	37.14319969327818	40.0	35.0	41.0	31.0	41.0
34	37.054330857105334	40.0	35.0	41.0	31.0	41.0
35	36.92847005543446	40.0	35.0	41.0	31.0	41.0
36	36.822149001565386	40.0	35.0	41.0	31.0	41.0
37	36.827004018310106	40.0	35.0	41.0	31.0	41.0
38	36.655395486867945	39.0	35.0	41.0	30.0	41.0
39	36.595300886061736	39.0	35.0	41.0	30.0	41.0
40	36.51312146650314	39.0	35.0	41.0	30.0	41.0
41	36.326855741184396	39.0	35.0	41.0	29.0	41.0
42	36.3184579602575	39.0	35.0	41.0	29.0	41.0
43	36.1205726038946	39.0	35.0	41.0	28.0	41.0
44	36.072793477501065	39.0	35.0	41.0	28.0	41.0
45	36.070833042074696	39.0	35.0	41.0	28.0	41.0
46	35.8806941064079	39.0	35.0	41.0	28.0	41.0
47	35.871184247038165	39.0	35.0	41.0	28.0	41.0
48	35.772399907644534	38.0	35.0	41.0	28.0	41.0
49	35.53042646619599	38.0	35.0	41.0	26.0	41.0
50	35.340877461665066	38.0	35.0	40.0	26.0	41.0
51	34.16770460654665	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	7.0
10	26.0
11	16.0
12	13.0
13	20.0
14	21.0
15	41.0
16	50.0
17	116.0
18	221.0
19	515.0
20	926.0
21	1625.0
22	2618.0
23	3860.0
24	5494.0
25	7632.0
26	10088.0
27	11344.0
28	12398.0
29	13589.0
30	15708.0
31	18655.0
32	23066.0
33	30495.0
34	50756.0
35	66085.0
36	67438.0
37	100272.0
38	177373.0
39	323455.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09487617800881	18.082819129443813	22.193908352026842	19.62839634052054
2	29.476115732414865	25.811870219386602	27.071696226770797	17.64031782142774
3	31.212652698961424	24.68411676611825	28.061234216429533	16.041996318490792
4	27.89759981698366	25.53279148635109	28.198496469945283	18.371112226719962
5	24.705299212648463	31.962405393898187	25.306033396245198	18.026261997208152
6	25.403578562516817	34.805725191648186	24.839066362486733	14.951629883348266
7	81.28626170065391	5.402900724227847	8.993643147796284	4.317194427321967
8	82.76702062534818	5.115137188114953	7.944476571155016	4.1733656153818455
9	75.25392457778088	8.676118274308447	10.424305586446623	5.645651561464046
10	33.9926369815861	37.7976398518076	15.590174734001428	12.61954843260487
11	26.489602596120648	28.489966934200968	28.587088451541977	16.43334201813641
12	25.79608929672159	22.302574302726814	33.00617044667425	18.895165953877342
13	26.396505743620374	22.859248997540718	32.05020663476591	18.694038624073002
14	19.546208447983325	29.829333028306102	30.094749083329624	20.529709440380945
15	18.73735672722728	26.1305601274336	36.126238908341435	19.005844236997685
16	21.887716087432665	28.39517548597828	29.980469784299146	19.736638642289908
17	22.556657748856676	24.7190678028931	28.269775402519443	24.454499045730785
18	26.314529675548464	24.300079010525558	28.516656816829027	20.86873449709695
19	26.127806409384668	26.499240609291892	26.344820574086665	21.02813240723677
20	25.05300907244185	25.02398912069546	27.01100851746175	22.91199328940094
21	26.35435267502526	27.09732698707235	27.8715454077515	18.676774930150884
22	22.628360330361435	27.309363276839747	27.448637862775875	22.61363853002294
23	20.81588429300407	26.582699448620918	27.647011474531286	24.954404783843724
24	20.966809224531815	24.73950886379475	28.57416715915855	25.71951475251489
25	21.23211936732269	27.906602356759002	25.951462542020675	24.909815733897634
26	21.019447604159385	26.709794127802173	26.53355617267083	25.73720209536761
27	22.252160079984918	25.958346837142997	26.71318331924701	25.076309763625076
28	22.92427910838846	26.76846950469085	28.57522628148506	21.73202510543563
29	22.8116944050804	25.722268470563815	29.4444479748522	22.02158914950359
30	25.397753389721007	26.408156089211992	27.213089057359944	20.981001463707056
31	23.47258673682293	29.286109187038882	25.128418582089395	22.112885494048793
32	24.21767929352304	29.804443653633108	25.57992242988081	20.397954622963045
33	23.416347341285224	28.08538220547397	26.936552217908066	21.561718235332744
34	25.09272615968599	27.004441959037383	26.367168055176037	21.53566382610059
35	22.953193147902194	28.76745698374671	27.018528285979976	21.26082158237112
36	23.069167042655092	28.683786319952382	28.05456174577251	20.192484891620012
37	23.01758778535403	30.29248722168913	26.215289913554436	20.4746350794024
38	22.631431785108315	27.746251236525314	26.1359616512988	23.486355327067564
39	25.880607258377132	26.284556513708218	25.750652948914293	22.084183279000356
40	25.92212485357634	25.05502140486222	27.998110525769505	21.02474321579194
41	22.237438279646423	26.2374255701785	27.68238616023673	23.84274998993834
42	24.3041036753663	26.881160120231566	27.253441617999997	21.56129458640214
43	21.837195952458117	28.174030744202895	27.413369089303075	22.575404214035913
44	21.610967423515483	27.00348874894353	26.55897510850708	24.82656871903391
45	21.45707694947351	25.812505692782505	26.87194575599093	25.85847160175306
46	22.52265992217569	27.79867779168758	25.878912662654713	23.799749623482015
47	21.513316345011216	25.56520062954231	27.92884392561572	24.99263909983075
48	21.488003321407618	25.29533626074744	27.901518569591747	25.31514184825319
49	23.72073909792433	24.86914543655963	28.42355996432876	22.986555501187276
50	21.273531050289247	24.727540781505184	30.41672227058881	23.582205897616763
51	22.763822075922125	24.18505832586652	27.78215548339402	25.268964114817333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	346.0
1	702.5
2	1059.0
3	4233.0
4	7407.0
5	5034.0
6	2661.0
7	2562.0
8	2463.0
9	2470.5
10	2478.0
11	2456.0
12	2434.0
13	2404.0
14	2374.0
15	2321.5
16	2269.0
17	2328.5
18	2388.0
19	2578.0
20	2768.0
21	2952.5
22	3137.0
23	3295.5
24	3454.0
25	4268.0
26	6164.5
27	7247.0
28	9099.0
29	10951.0
30	13698.0
31	16445.0
32	18237.0
33	20029.0
34	23122.0
35	26215.0
36	28185.0
37	30155.0
38	32717.0
39	35279.0
40	38774.5
41	42270.0
42	46375.5
43	50481.0
44	57581.0
45	64681.0
46	75373.0
47	86065.0
48	88002.5
49	89940.0
50	87425.5
51	84911.0
52	74770.0
53	64629.0
54	58353.0
55	52077.0
56	50700.5
57	49324.0
58	47364.5
59	45405.0
60	43529.0
61	41653.0
62	36291.5
63	30930.0
64	25639.5
65	20349.0
66	16323.0
67	12297.0
68	10126.5
69	7956.0
70	6640.0
71	5324.0
72	4563.0
73	3802.0
74	3252.5
75	1974.0
76	1245.0
77	982.5
78	720.0
79	602.0
80	484.0
81	346.5
82	209.0
83	129.5
84	50.0
85	32.0
86	14.0
87	8.5
88	3.0
89	6.5
90	10.0
91	6.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	944178.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.24164351430783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51935050916218	27.369453795851168
2	8.577770246135481	6.217449828161573
3	3.218507706152176	3.4993202680325934
4	1.8474212670640224	2.678143319267407
5	1.289521568592412	2.3367190496468617
6	0.9743566827548299	2.1187372531310444
7	0.8077046007523536	2.0490779543733195
8	0.7206657889270335	2.089449009220077
9	0.6252790086646637	2.0395025036104077
>10	6.310585031178875	44.31564059211043
>50	0.07928774018589954	1.8975798746595482
>100	0.025453831558717197	1.5531740600555055
>500	0.0023405822122011207	0.5882720975538771
>1k	0.0017554366591508402	1.247480394326129
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4086	0.43275738261217694	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	2267	0.24010303141992292	No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	1715	0.18163947899654515	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	1445	0.15304317618076252	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1189	0.12592964462209455	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	1063	0.11258470330806267	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0591223265104673E-4	0.0	0.0	0.12232862871195897	0.0
2	1.0591223265104673E-4	0.0	0.0	0.5523322932752087	0.0
3	1.0591223265104673E-4	0.0	0.0	0.8829902836117766	0.0
4	1.0591223265104673E-4	0.0	0.0	1.2606733052454093	0.0
5	1.0591223265104673E-4	0.0	0.0	2.2199203963659393	0.0
6	1.0591223265104673E-4	0.0	0.0	3.2988483103821524	0.0
7	1.0591223265104673E-4	0.0	0.0	4.051354723367839	0.0
8	1.0591223265104673E-4	0.0	0.0	5.3687969853142095	0.0
9	1.0591223265104673E-4	0.0	0.0	6.0132729209958296	0.0
10	1.0591223265104673E-4	0.0	0.0	7.093154045105901	0.0
11	1.0591223265104673E-4	0.0	0.0	7.990336567892919	0.0
12	1.0591223265104673E-4	0.0	0.0	9.023722221869182	0.0
13	1.0591223265104673E-4	0.0	0.0	9.699865915113463	0.0
14	1.0591223265104673E-4	0.0	0.0	10.072994710743101	0.0
15	1.0591223265104673E-4	0.0	0.0	10.349637462427635	0.0
16	1.0591223265104673E-4	0.0	0.0	10.78313623066837	0.0
17	2.1182446530209346E-4	0.0	0.0	11.299352452609572	0.0
18	2.1182446530209346E-4	0.0	0.0	11.94774714089928	0.0
19	2.1182446530209346E-4	0.0	0.0	12.319816814202406	0.0
20	3.177366979531402E-4	0.0	0.0	12.738170133174041	0.0
21	3.177366979531402E-4	0.0	0.0	13.174634443929005	0.0
22	3.177366979531402E-4	0.0	0.0	13.622855012508234	0.0
23	3.177366979531402E-4	0.0	0.0	14.064297198197798	0.0
24	3.177366979531402E-4	0.0	0.0	14.409994725570813	0.0
25	4.236489306041869E-4	0.0	0.0	14.745312854144029	0.0
26	5.295611632552337E-4	0.0	0.0	15.096517817614899	0.0
27	5.295611632552337E-4	0.0	0.0	15.487969429493168	0.0
28	6.354733959062804E-4	0.0	0.0	15.835361552588601	0.0
29	6.354733959062804E-4	0.0	0.0	16.22162346506697	0.0
30	6.354733959062804E-4	0.0	0.0	16.737098301379614	0.0
31	6.354733959062804E-4	0.0	0.0	17.138823399825032	0.0
32	6.354733959062804E-4	0.0	0.0	17.57751186746567	0.0
33	6.354733959062804E-4	0.0	0.0	18.14435413661407	0.0
34	7.413856285573271E-4	0.0	0.0	18.772837325165384	0.0
35	7.413856285573271E-4	0.0	0.0	19.250607406654254	0.0
36	7.413856285573271E-4	0.0	0.0	19.691202294482608	0.0
37	7.413856285573271E-4	0.0	0.0	20.093351041858632	0.0
38	7.413856285573271E-4	0.0	0.0	20.50386685561409	0.0
39	7.413856285573271E-4	0.0	0.0	21.009597766522838	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGGT	20	7.032646E-4	45.0	40
CAGTACG	25	3.890275E-5	45.0	1
GCCGATT	30	2.1650885E-6	44.999996	9
CTCGACG	30	2.1650885E-6	44.999996	1
CGTTTTT	2410	0.0	40.79875	1
AGTACGG	200	0.0	39.375	2
GGCGATA	350	0.0	39.214287	8
TATGCGG	190	0.0	39.078945	2
GCGATAA	100	0.0	38.25	9
TACGCGG	65	9.094947E-12	38.076927	2
CGCGAGG	90	0.0	37.500004	2
TAGCGCG	30	1.13976756E-4	37.499996	1
GTTAACG	30	1.13976756E-4	37.499996	1
ACGTAGT	30	1.13976756E-4	37.499996	12
AGTAACG	30	1.13976756E-4	37.499996	1
ACACGAC	115	0.0	37.173912	26
CACGACC	115	0.0	37.173912	27
CGGAAGG	195	0.0	36.92308	2
TTATGCG	55	2.748493E-9	36.818184	1
CTCGATG	55	2.748493E-9	36.818184	1
>>END_MODULE