FastQCFastQC Report
Sat 14 Jan 2017
SRR2935731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033602
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC321613.1115458367921116No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCG144901.4018935721873604No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGC142761.3811892778845243No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140821.362419964357654No Hit
GAACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT105861.0241853247187989No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC58420.5652078846596659No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTT42430.41050617162118497No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTT38440.37190330514066344No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCT33880.3277857434486388No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC29450.28492591926099214No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTC29280.28328118560142107No Hit
CGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG26470.25609470569909887No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCT22580.21845932960656037No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATGCGGT21320.2062689507179746No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC19950.19301433240260757No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTA19870.19224034009222118No Hit
CGTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT15070.1458008014690374No Hit
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT12160.11764683117873224Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG406.8139343E-945.0000041
TAGCACG207.032955E-445.0000041
CCACGCA406.8139343E-945.00000440
CGTTTTT80750.043.885451
CCGATGA10000.040.50000418
CGATGAA10050.040.29850819
GTAAGCG451.9279469E-840.01
CGGCGAA2500.039.60000231
TACGGCT17200.039.3757
CGTTATT2200.038.8636361
ACGATAG356.2476793E-638.5714261
CTACGCG356.2476793E-638.5714261
TTGAACG356.2476793E-638.5714261
ACGGCTG17700.038.262718
TACGCGG659.094947E-1238.076922
CGTTTCT4850.038.0412371
ACGGGCG1550.037.7419365
CGTTTTC3100.037.7419361
GAATCTG38100.037.6771661
CGCATCG301.1398426E-437.49999621
Produced by FastQC (version 0.11.5)