##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935731.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1033602
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.934792115340333	33.0	31.0	34.0	30.0	34.0
2	32.00234422921008	33.0	31.0	34.0	30.0	34.0
3	31.982747711401487	33.0	31.0	34.0	30.0	34.0
4	35.69730418478292	37.0	35.0	37.0	33.0	37.0
5	34.8066789731444	37.0	35.0	37.0	33.0	37.0
6	35.21367315465721	37.0	35.0	37.0	33.0	37.0
7	35.7614081629099	37.0	35.0	37.0	33.0	37.0
8	35.66972393629269	37.0	35.0	37.0	33.0	37.0
9	37.570709035005734	39.0	37.0	39.0	35.0	39.0
10	37.29542125498983	39.0	37.0	39.0	34.0	39.0
11	37.20958550776798	39.0	37.0	39.0	34.0	39.0
12	37.25034297534254	39.0	37.0	39.0	34.0	39.0
13	37.212522808585895	39.0	37.0	39.0	33.0	39.0
14	38.461882813694245	40.0	38.0	41.0	34.0	41.0
15	38.4125175841378	40.0	38.0	41.0	33.0	41.0
16	38.497523224606766	40.0	38.0	41.0	34.0	41.0
17	38.40050909344215	40.0	38.0	41.0	34.0	41.0
18	38.28796674155042	40.0	38.0	41.0	34.0	41.0
19	38.10388524790006	40.0	37.0	41.0	34.0	41.0
20	37.87935975356085	40.0	36.0	41.0	33.0	41.0
21	37.97760936995091	40.0	36.0	41.0	34.0	41.0
22	38.04825842055259	40.0	36.0	41.0	34.0	41.0
23	38.03357868889572	40.0	36.0	41.0	34.0	41.0
24	37.98947176959797	40.0	36.0	41.0	34.0	41.0
25	37.89528948279899	40.0	36.0	41.0	33.0	41.0
26	37.80691504079907	40.0	36.0	41.0	33.0	41.0
27	37.80346787254668	40.0	36.0	41.0	33.0	41.0
28	37.700518187851806	40.0	36.0	41.0	33.0	41.0
29	37.61090922811682	40.0	36.0	41.0	33.0	41.0
30	37.516340912653035	40.0	36.0	41.0	33.0	41.0
31	37.3541962960598	40.0	36.0	41.0	33.0	41.0
32	37.13612202762766	40.0	36.0	41.0	32.0	41.0
33	36.81642547131294	40.0	35.0	41.0	31.0	41.0
34	36.520695586889346	40.0	35.0	41.0	30.0	41.0
35	36.39330902997479	40.0	35.0	41.0	30.0	41.0
36	36.24447127617787	40.0	35.0	41.0	29.0	41.0
37	36.105142017914055	39.0	35.0	41.0	28.0	41.0
38	36.02402859127594	39.0	35.0	41.0	27.0	41.0
39	35.92914874390723	39.0	35.0	41.0	27.0	41.0
40	35.767134738516376	39.0	35.0	41.0	26.0	41.0
41	35.69930592239566	39.0	35.0	41.0	25.0	41.0
42	35.72608121888309	39.0	35.0	41.0	26.0	41.0
43	35.67308209542938	39.0	35.0	41.0	25.0	41.0
44	35.56359217571173	39.0	35.0	41.0	25.0	41.0
45	35.549399091720026	39.0	35.0	41.0	25.0	41.0
46	35.43534745482304	39.0	35.0	41.0	24.0	41.0
47	35.21102029601336	39.0	35.0	41.0	23.0	41.0
48	35.27811091696804	39.0	35.0	41.0	24.0	41.0
49	35.296590950868904	39.0	35.0	41.0	24.0	41.0
50	35.17690851991385	39.0	35.0	40.0	24.0	41.0
51	34.08224829286321	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	13.0
10	15.0
11	16.0
12	13.0
13	15.0
14	20.0
15	33.0
16	54.0
17	119.0
18	322.0
19	735.0
20	1523.0
21	2782.0
22	4034.0
23	5287.0
24	7476.0
25	11217.0
26	15606.0
27	17146.0
28	16641.0
29	15917.0
30	17190.0
31	20893.0
32	26775.0
33	34711.0
34	52148.0
35	68911.0
36	73400.0
37	105348.0
38	189668.0
39	345301.0
40	264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64881259904683	15.921312071764568	17.083364776770942	23.346510552417662
2	31.267257609795646	32.13712821763116	21.49125098442147	15.104363188151726
3	25.08218830845916	31.259807933808176	30.28631910541969	13.371684652312979
4	22.44384202042953	21.960870818748415	39.280883744419995	16.314403416402058
5	26.114307054359415	26.390041814934566	29.489494021876894	18.006157108829125
6	21.96686925915391	34.00303017989516	30.06428006137759	13.965820499573336
7	70.88318327557415	4.112221145082923	20.852707328352693	4.151888250990226
8	70.27705054750281	8.371887825294454	15.971428073862088	5.379633553340647
9	64.41879949922698	5.871602415630001	16.708172004311137	13.001426080831887
10	32.01580492297809	29.4547611169483	23.641498371713677	14.88793558835993
11	23.130663446858655	23.086449136127833	34.88489766854166	18.89798974847185
12	19.343615821176815	20.569135895634876	38.300719232354425	21.78652905083388
13	20.137731931633258	22.671589257760726	40.9592860694929	16.23139274111312
14	17.24416167925372	29.302671627957377	33.93327412292159	19.519892569867316
15	14.954885923208353	25.276557127404942	41.21034982517449	18.55820712421222
16	17.85571235349777	27.216375355310845	32.4530138293076	22.47489846188378
17	17.93117660376044	26.53197265485168	33.59755495829149	21.939295783096394
18	19.408534426210476	25.194610691542778	33.75815836269667	21.638696519550077
19	17.768734967618098	27.366723361603402	31.454757246986752	23.40978442379175
20	19.896730075986696	26.82860520780726	34.94410808028622	18.330556635919823
21	18.65379517454494	29.631424861794	33.902217681467334	17.812562282193724
22	18.025990661782775	22.60821863734784	35.503414273579196	23.862376427290194
23	17.771637438782044	27.236692653458487	35.1273507597702	19.864319147989264
24	19.676432514642965	24.901170856867537	33.311081054409726	22.11131557407977
25	17.558789553425786	32.13857945321313	30.455629923316714	19.847001070044367
26	18.57987890890304	25.491339993537167	32.54144245076925	23.387338646790546
27	21.21919268732065	26.46608655943003	29.818247255713516	22.496473497535803
28	16.133676211926833	27.821637342033007	33.81030609460895	22.234380351431206
29	18.341876273459224	28.70263408933032	31.77557705964191	21.17991257756854
30	17.65428085471971	31.895932863906996	32.27141588348319	18.1783703978901
31	19.28034194980273	29.710081830337014	29.552574395173387	21.457001824686873
32	21.423720155340256	28.656678295901127	32.34920211067703	17.570399438081584
33	22.4796391647849	26.07589768595649	28.805188070456524	22.639275078802093
34	20.90224283621742	26.91093863982461	29.36700973875824	22.81980878519972
35	23.225671002958588	26.544163033740258	28.88684425920228	21.34332170409887
36	24.019013121104642	29.83150187402888	27.30180475656974	18.847680248296733
37	19.828522003633893	27.297934795017813	32.762997749617355	20.11054545173094
38	22.34747997778642	27.672160077089636	28.33701947171155	21.643340473412398
39	23.020466291667393	24.49695337276824	30.48949208689612	21.99308824866825
40	24.721314393741498	24.41790940807003	31.69730708725409	19.163469110934383
41	21.071747152192046	27.363337145245463	28.427479822987962	23.137435879574536
42	22.691519559753175	25.630174864212723	31.956401013155933	19.721904562878166
43	24.57725507497083	24.8976878914708	28.87213840530494	21.652918628253428
44	21.534110808609118	24.468799402477938	29.8930342627046	24.10405552620835
45	20.131830240266563	23.611989914880198	29.489300523799294	26.766879321053942
46	24.653783564660284	25.321158434290957	28.53380701662729	21.49125098442147
47	19.211069638023144	24.607731022192294	34.78660064512259	21.39459869466197
48	20.084519960294195	25.31167702848872	30.36342808934193	24.24037492187515
49	20.08935741223411	22.446841240632274	35.3490028076571	22.11479853947651
50	20.097677829570763	22.786333617775504	34.01376932320178	23.102219229451958
51	19.4412356013243	22.26263107076031	30.580629681444115	27.715503646471273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	318.0
1	701.5
2	1085.0
3	11865.5
4	22646.0
5	15024.5
6	7403.0
7	7113.5
8	6824.0
9	6780.5
10	6737.0
11	6633.0
12	6529.0
13	6461.5
14	6394.0
15	6121.0
16	5848.0
17	5717.5
18	5587.0
19	5308.0
20	5029.0
21	5122.0
22	5215.0
23	5762.0
24	6309.0
25	7098.0
26	9028.0
27	10169.0
28	12387.5
29	14606.0
30	16893.0
31	19180.0
32	21662.0
33	24144.0
34	27854.0
35	31564.0
36	33928.5
37	36293.0
38	39010.0
39	41727.0
40	44541.0
41	47355.0
42	51594.5
43	55834.0
44	62253.5
45	68673.0
46	120233.5
47	171794.0
48	134940.0
49	98086.0
50	94811.5
51	91537.0
52	77408.5
53	63280.0
54	54908.0
55	46536.0
56	40442.5
57	34349.0
58	29944.0
59	25539.0
60	22420.0
61	19301.0
62	16480.0
63	13659.0
64	11647.0
65	9635.0
66	7735.5
67	5836.0
68	4938.0
69	4040.0
70	3208.5
71	2377.0
72	2022.5
73	1668.0
74	1424.0
75	970.5
76	761.0
77	534.0
78	307.0
79	261.0
80	215.0
81	157.5
82	100.0
83	60.5
84	21.0
85	14.5
86	8.0
87	7.0
88	6.0
89	6.0
90	6.0
91	3.5
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1033602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.10124003088258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16749430083695	27.497409943053952
2	8.712980750191951	6.290988188942779
3	3.2568075635252223	3.5272437475565375
4	1.87636263968407	2.709560721608602
5	1.3640063028194989	2.4621159470861715
6	1.070408994233809	2.318585521923021
7	0.8593555605778428	2.1716660955006053
8	0.7352022527051163	2.123337039692239
9	0.659756000868771	2.14362087742608
>10	5.200771074977005	32.99747457026579
>50	0.06234042418002679	1.4906824729225263
>100	0.027290246596825583	1.8031306680440693
>500	0.002140411497162337	0.5302757320491391
>1k	0.003478168682888798	3.102064737794199
>5k	2.675514371452921E-4	0.5642751961076632
>10k+	0.0013377571857264609	8.267568540026605
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC	32161	3.1115458367921116	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCG	14490	1.4018935721873604	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGC	14276	1.3811892778845243	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14082	1.362419964357654	No Hit
GAACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT	10586	1.0241853247187989	No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	5842	0.5652078846596659	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTT	4243	0.41050617162118497	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTT	3844	0.37190330514066344	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCT	3388	0.3277857434486388	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC	2945	0.28492591926099214	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTC	2928	0.28328118560142107	No Hit
CGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	2647	0.25609470569909887	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCT	2258	0.21845932960656037	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATGCGGT	2132	0.2062689507179746	No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	1995	0.19301433240260757	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTA	1987	0.19224034009222118	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT	1507	0.1458008014690374	No Hit
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT	1216	0.11764683117873224	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.902471163948986E-4	0.0	0.0	0.1283859744853435	0.0
2	2.902471163948986E-4	0.0	0.0	0.9996110688640308	0.0
3	2.902471163948986E-4	0.0	0.0	1.5210883879868653	0.0
4	2.902471163948986E-4	0.0	0.0	3.0094756008599055	0.0
5	2.902471163948986E-4	0.0	0.0	7.215446564538381	0.0
6	2.902471163948986E-4	0.0	0.0	9.122466868291664	0.0
7	2.902471163948986E-4	0.0	0.0	10.437383054599353	0.0
8	2.902471163948986E-4	0.0	0.0	12.031903963034127	0.0
9	3.8699615519319816E-4	0.0	0.0	12.766809661746011	0.0
10	3.8699615519319816E-4	0.0	0.0	15.2936043080412	0.0
11	3.8699615519319816E-4	0.0	0.0	16.293699122099223	0.0
12	3.8699615519319816E-4	0.0	0.0	18.727517942109245	0.0
13	3.8699615519319816E-4	0.0	0.0	19.366351845294417	0.0
14	4.8374519399149767E-4	0.0	0.0	19.722291559033362	0.0
15	4.8374519399149767E-4	0.0	0.0	20.2207426069222	0.0
16	4.8374519399149767E-4	0.0	0.0	20.73622148563954	0.0
17	4.8374519399149767E-4	0.0	0.0	21.31526448284736	0.0
18	4.8374519399149767E-4	0.0	0.0	22.013308797777093	0.0
19	4.8374519399149767E-4	0.0	0.0	22.658624886561753	0.0
20	4.8374519399149767E-4	0.0	0.0	23.120021052590843	0.0
21	4.8374519399149767E-4	0.0	0.0	23.553843742562417	0.0
22	4.8374519399149767E-4	0.0	0.0	24.073386080909287	0.0
23	5.804942327897972E-4	0.0	0.0	24.533234262317603	0.0
24	5.804942327897972E-4	0.0	0.0	24.936581005067715	0.0
25	5.804942327897972E-4	0.0	0.0	25.28797351398314	0.0
26	5.804942327897972E-4	0.0	0.0	25.63888227770457	0.0
27	5.804942327897972E-4	0.0	0.0	26.04909820220936	0.0
28	6.772432715880967E-4	0.0	0.0	26.433482133355007	0.0
29	6.772432715880967E-4	0.0	0.0	26.8376996174543	0.0
30	6.772432715880967E-4	0.0	0.0	27.409292938674653	0.0
31	6.772432715880967E-4	0.0	0.0	27.82879677090408	0.0
32	6.772432715880967E-4	0.0	0.0	28.26513493588441	0.0
33	6.772432715880967E-4	0.0	0.0	28.649712365107654	0.0
34	6.772432715880967E-4	0.0	0.0	29.02713036545982	0.0
35	6.772432715880967E-4	0.0	0.0	29.42554290723122	0.0
36	6.772432715880967E-4	0.0	0.0	29.794350243130335	0.0
37	6.772432715880967E-4	0.0	0.0	30.160351856904303	0.0
38	6.772432715880967E-4	0.0	0.0	30.54560652939913	0.0
39	6.772432715880967E-4	0.0	0.0	30.973721026081606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACG	40	6.8139343E-9	45.000004	1
TAGCACG	20	7.032955E-4	45.000004	1
CCACGCA	40	6.8139343E-9	45.000004	40
CGTTTTT	8075	0.0	43.88545	1
CCGATGA	1000	0.0	40.500004	18
CGATGAA	1005	0.0	40.298508	19
GTAAGCG	45	1.9279469E-8	40.0	1
CGGCGAA	250	0.0	39.600002	31
TACGGCT	1720	0.0	39.375	7
CGTTATT	220	0.0	38.863636	1
ACGATAG	35	6.2476793E-6	38.571426	1
CTACGCG	35	6.2476793E-6	38.571426	1
TTGAACG	35	6.2476793E-6	38.571426	1
ACGGCTG	1770	0.0	38.26271	8
TACGCGG	65	9.094947E-12	38.07692	2
CGTTTCT	485	0.0	38.041237	1
ACGGGCG	155	0.0	37.741936	5
CGTTTTC	310	0.0	37.741936	1
GAATCTG	3810	0.0	37.677166	1
CGCATCG	30	1.1398426E-4	37.499996	21
>>END_MODULE