FastQCFastQC Report
Sat 14 Jan 2017
SRR2935730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131359
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC147281.3017972190966793No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64510.5701992029055322No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG57820.5110667789799701No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC54590.4825170436616494No Hit
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT47520.42002582734569666No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC40640.3592140072249392TruSeq Adapter, Index 23 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT24220.21407882025068964No Hit
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC23960.21178069914147502TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT17170.15176438248159957No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG17170.15176438248159957TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT16090.14221834095101554TruSeq Adapter, Index 20 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT15820.13983183056836956No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC14910.13178840668611821No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC14750.13037417831121687No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT12110.10703941012534482No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.033238E-445.00000439
CGTTTTT32000.041.343751
GCTACGA1400.038.5714310
AGTAACG301.1399108E-437.4999961
CGAATAT1450.037.24138314
CTCGTAA1650.036.8181833
GCGATAT1100.036.818189
GCGTAAG1350.036.6666641
CGTAAGG2150.036.6279072
GACACGA2950.036.6101725
TTACGGG2100.036.428573
ATCTCGT250.002107094736.014
ACACGAC3000.036.026
TACGAAT1500.036.012
CATCGAA250.002107094736.012
TCGCGAT250.002107094736.043
ACGACCA3000.036.028
TGCACGG2700.035.8333322
AGTACGG1450.035.689662
TACGGCT8000.035.43757
Produced by FastQC (version 0.11.5)