##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935730.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1131359
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07048249052688	33.0	31.0	34.0	30.0	34.0
2	32.11401332379908	33.0	31.0	34.0	30.0	34.0
3	32.1302963957506	33.0	31.0	34.0	30.0	34.0
4	35.80298738066343	37.0	35.0	37.0	35.0	37.0
5	34.870487617104736	37.0	35.0	37.0	33.0	37.0
6	35.32403772807747	37.0	35.0	37.0	33.0	37.0
7	35.86524790097573	37.0	35.0	37.0	35.0	37.0
8	35.81635802605539	37.0	35.0	37.0	35.0	37.0
9	37.63974388324131	39.0	37.0	39.0	35.0	39.0
10	37.36910653470738	39.0	37.0	39.0	34.0	39.0
11	37.337828222518226	39.0	37.0	39.0	34.0	39.0
12	37.306929984204835	39.0	37.0	39.0	34.0	39.0
13	37.26338500864889	39.0	37.0	39.0	34.0	39.0
14	38.44888138955009	40.0	38.0	41.0	34.0	41.0
15	38.49027497019072	40.0	38.0	41.0	34.0	41.0
16	38.495559764849176	40.0	38.0	41.0	34.0	41.0
17	38.43714329403841	40.0	38.0	41.0	34.0	41.0
18	38.38793433384098	40.0	38.0	41.0	34.0	41.0
19	38.28270336825004	40.0	37.0	41.0	34.0	41.0
20	38.22604672787329	40.0	37.0	41.0	34.0	41.0
21	38.17017144867368	40.0	37.0	41.0	34.0	41.0
22	38.213465398693074	40.0	37.0	41.0	34.0	41.0
23	38.19787795032346	40.0	37.0	41.0	34.0	41.0
24	38.15829369810997	40.0	37.0	41.0	34.0	41.0
25	38.01219064859165	40.0	36.0	41.0	34.0	41.0
26	37.998935793147886	40.0	37.0	41.0	34.0	41.0
27	38.00876909981712	40.0	37.0	41.0	34.0	41.0
28	37.84750198654892	40.0	36.0	41.0	33.0	41.0
29	37.80707626845236	40.0	36.0	41.0	33.0	41.0
30	37.67160379684963	40.0	36.0	41.0	33.0	41.0
31	37.600442476702796	40.0	36.0	41.0	33.0	41.0
32	37.473734685453515	40.0	36.0	41.0	33.0	41.0
33	37.333680113916095	40.0	36.0	41.0	33.0	41.0
34	37.222974316728816	40.0	36.0	41.0	32.0	41.0
35	37.13613539115347	40.0	35.0	41.0	32.0	41.0
36	37.05831217146812	40.0	35.0	41.0	32.0	41.0
37	37.0310246349744	40.0	35.0	41.0	32.0	41.0
38	36.94913374092574	40.0	35.0	41.0	31.0	41.0
39	36.864611498207026	39.0	35.0	41.0	31.0	41.0
40	36.74720225852271	39.0	35.0	41.0	31.0	41.0
41	36.624286367103636	39.0	35.0	41.0	31.0	41.0
42	36.64686982646534	39.0	35.0	41.0	31.0	41.0
43	36.56251463947341	39.0	35.0	41.0	31.0	41.0
44	36.4376762813572	39.0	35.0	41.0	30.0	41.0
45	36.402193291430926	39.0	35.0	41.0	30.0	41.0
46	36.256804427241924	39.0	35.0	41.0	30.0	41.0
47	36.13391947206855	39.0	35.0	41.0	30.0	41.0
48	36.09263195855604	38.0	35.0	40.0	30.0	41.0
49	36.06103544498254	38.0	35.0	40.0	30.0	41.0
50	35.96247698564293	38.0	35.0	40.0	30.0	41.0
51	34.77391084527546	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	22.0
10	24.0
11	16.0
12	18.0
13	18.0
14	37.0
15	40.0
16	104.0
17	139.0
18	299.0
19	528.0
20	1012.0
21	1703.0
22	2649.0
23	3736.0
24	5321.0
25	7247.0
26	9575.0
27	11096.0
28	12351.0
29	13711.0
30	16072.0
31	20476.0
32	27026.0
33	37393.0
34	64437.0
35	83832.0
36	86054.0
37	127012.0
38	218821.0
39	380297.0
40	279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17227422948861	16.617360183637555	19.755179390449893	19.455186196423945
2	30.47591436493633	27.21267077912493	24.442639339060367	17.868775516878372
3	30.544062494751888	26.794059180154132	26.82552576149569	15.836352563598291
4	27.42515859245385	24.04029136640094	29.482507320841574	19.052042720303636
5	25.600362042463974	29.602893511255047	25.1087409036389	19.68800354264208
6	24.963340548844354	34.32031742355875	25.581181570129374	15.135160457467522
7	75.88068862315144	5.915452124391992	12.995167758421507	5.208691494035049
8	75.67739329425937	6.967284478224861	11.280592632400502	6.074729595115255
9	68.4900195251905	7.603245300563305	14.079792532697402	9.826942641548793
10	37.11315329616859	25.713677091002946	20.42914760036381	16.744022012464654
11	29.275853199559116	24.62030177865735	26.50564498094769	19.598200040835845
12	25.86261301673474	20.862431818724207	31.46260382425031	21.812351340290746
13	23.969138001288716	22.060725198632795	33.874481928371104	20.09565487170739
14	20.38601363492932	26.52279249999337	30.70316318692829	22.388030678149022
15	19.001660834447776	24.268070524033487	35.58525631563456	21.14501232588418
16	22.01529311208909	24.833319927626864	31.08765652635459	22.06373043392946
17	21.82057154271986	25.429593966194634	28.9892067858213	23.760627705264202
18	23.724564881704215	24.448473031106836	28.77459762993002	23.052364457258925
19	23.314350263709397	27.209488765281403	26.20370722290626	23.272453748102944
20	24.93593987408064	25.472197595988543	28.00790907218664	21.583953457744183
21	24.449268534567718	27.840146231213964	27.493483500816275	20.21710173340204
22	23.100183054185276	24.290521399485044	27.811684885169075	24.797610661160604
23	22.21823488388743	26.828972943159513	28.2261421882886	22.72664998466446
24	23.31709033118577	23.232766964332278	29.343470993734083	24.10667171074787
25	22.935955784149858	27.2035666839615	26.301023812954156	23.559453718934485
26	22.151589371720206	24.893071076466445	28.491928733496618	24.46341081831673
27	25.074092308453817	24.243498306019575	26.266286828495637	24.41612255703097
28	21.71627220007089	25.67284124667767	29.519719204956164	23.09116734829528
29	23.59675399232251	24.46579732869938	28.63724069901773	23.300207979960383
30	24.696758500175452	24.040468144947802	28.775304744117474	22.487468610759272
31	24.42080718852283	24.334450868380415	27.867988852344833	23.37675309075192
32	24.05646660343887	26.053091900979265	27.82458971909005	22.06585177649181
33	23.13836722030761	24.883348256389	26.15138077303491	25.82690375026848
34	21.736424954413234	24.075028350859455	30.924755095420643	23.263791599306675
35	22.129845610456098	24.359376643488055	28.101071366383263	25.409706379672585
36	21.01119096590914	25.53901988670263	27.643568487102677	25.80622066028555
37	22.151500982446773	25.53990377943694	28.770885280445906	23.537709957670376
38	21.42140558390396	24.84516409026666	27.21205205421091	26.521378271618467
39	22.930122092103392	24.165892523946862	27.255627966012558	25.64835741793719
40	23.318681338107535	22.768723278817777	29.236078026515017	24.67651735655968
41	20.000901570589	24.89148006954468	28.6695911730936	26.43802718677272
42	22.33482033554336	23.97072900821048	28.89940328401506	24.795047372231096
43	23.361196578628004	23.062264055883233	28.2924341433621	25.284105222126662
44	23.345905234324384	23.44967424133277	28.126439087858053	25.07798143648479
45	22.392096584726865	22.33084281823895	28.187516075799106	27.08954452123508
46	23.668702860895614	23.87208657906111	27.48844531223069	24.970765247812587
47	21.763030125716064	23.264233545673832	30.777233398063746	24.19550293054636
48	21.583511511377026	22.916952090362123	29.280007495410388	26.219528902850463
49	21.67914870522973	22.295133551772693	30.50039819367681	25.525319549320773
50	20.43029666091842	22.268174823376135	31.711331239686075	25.59019727601937
51	20.516741370334262	21.53268767915401	29.32331823939174	28.627252711119986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	340.0
1	860.0
2	1380.0
3	6146.0
4	10912.0
5	7145.5
6	3379.0
7	3267.5
8	3156.0
9	3036.0
10	2916.0
11	2948.5
12	2981.0
13	2956.0
14	2931.0
15	2893.0
16	2855.0
17	2874.0
18	2893.0
19	2843.5
20	2794.0
21	3045.0
22	3296.0
23	3789.0
24	4282.0
25	5158.0
26	6584.0
27	7134.0
28	9409.5
29	11685.0
30	13141.0
31	14597.0
32	16271.0
33	17945.0
34	21657.0
35	25369.0
36	27243.5
37	29118.0
38	31171.0
39	33224.0
40	36799.0
41	40374.0
42	45311.0
43	50248.0
44	57489.0
45	64730.0
46	95095.0
47	125460.0
48	116750.0
49	108040.0
50	107495.0
51	106950.0
52	94256.0
53	81562.0
54	73954.5
55	66347.0
56	62502.5
57	58658.0
58	56364.0
59	54070.0
60	52153.0
61	50236.0
62	45777.0
63	41318.0
64	35803.0
65	30288.0
66	25617.0
67	20946.0
68	17781.5
69	14617.0
70	12618.5
71	10620.0
72	9115.0
73	7610.0
74	6076.0
75	3749.0
76	2956.0
77	2128.0
78	1300.0
79	979.0
80	658.0
81	508.5
82	359.0
83	253.0
84	147.0
85	94.0
86	41.0
87	33.0
88	25.0
89	24.5
90	24.0
91	16.0
92	8.0
93	4.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1131359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.18261230408172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.67526563718485	23.710625104017545
2	8.973303767100022	5.775687124466721
3	3.3282757260259666	3.213378219953394
4	1.9114855763073395	2.4606639690857333
5	1.343247353635321	2.1614604405264646
6	1.081131025991622	2.087617239564148
7	0.8736542492587698	1.9681533194195993
8	0.7372897974110229	1.8982329364667125
9	0.681047096485921	1.9726087200324236
>10	7.250892547952474	44.93028142531353
>50	0.09599727640564741	2.0554878690972456
>100	0.04208364645357753	2.229208505200035
>500	0.0019253955875352066	0.4144523771934629
>1k	0.0033006781500603544	2.252312320655803
>5k	8.251695375150886E-4	1.5661024043799112
>10k+	2.7505651250502955E-4	1.3037280246273646
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	14728	1.3017972190966793	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6451	0.5701992029055322	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	5782	0.5110667789799701	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	5459	0.4825170436616494	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	4752	0.42002582734569666	No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	4064	0.3592140072249392	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT	2422	0.21407882025068964	No Hit
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	2396	0.21178069914147502	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT	1717	0.15176438248159957	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	1717	0.15176438248159957	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	1609	0.14221834095101554	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	1582	0.13983183056836956	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	1491	0.13178840668611821	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC	1475	0.13037417831121687	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	1211	0.10703941012534482	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.838927343133347E-5	0.0	0.0	0.14849397936464023	0.0
2	8.838927343133347E-5	0.0	0.0	0.8267048744032619	0.0
3	8.838927343133347E-5	0.0	0.0	1.2946376879487413	0.0
4	8.838927343133347E-5	0.0	0.0	2.1601454533883584	0.0
5	8.838927343133347E-5	0.0	0.0	4.507145830810556	0.0
6	8.838927343133347E-5	0.0	0.0	6.301271302919763	0.0
7	8.838927343133347E-5	0.0	0.0	7.455016489018958	0.0
8	8.838927343133347E-5	0.0	0.0	9.19973235728005	0.0
9	8.838927343133347E-5	0.0	0.0	10.102982342474847	0.0
10	8.838927343133347E-5	0.0	0.0	11.870502643281222	0.0
11	8.838927343133347E-5	0.0	0.0	12.894757543803514	0.0
12	8.838927343133347E-5	0.0	0.0	14.365820221521197	0.0
13	8.838927343133347E-5	0.0	0.0	14.940085331004571	0.0
14	8.838927343133347E-5	0.0	0.0	15.228145973117286	0.0
15	8.838927343133347E-5	0.0	0.0	15.581791456116052	0.0
16	8.838927343133347E-5	0.0	0.0	16.078627562073578	0.0
17	8.838927343133347E-5	0.0	0.0	16.60639991373207	0.0
18	8.838927343133347E-5	0.0	0.0	17.26790523609217	0.0
19	8.838927343133347E-5	0.0	0.0	17.71612724166246	0.0
20	1.7677854686266693E-4	0.0	0.0	18.124750852735517	0.0
21	1.7677854686266693E-4	0.0	0.0	18.548754197385623	0.0
22	1.7677854686266693E-4	0.0	0.0	19.032243523055016	0.0
23	1.7677854686266693E-4	0.0	0.0	19.461638613384434	0.0
24	1.7677854686266693E-4	0.0	0.0	19.82916121231192	0.0
25	1.7677854686266693E-4	0.0	0.0	20.164244947890104	0.0
26	1.7677854686266693E-4	0.0	0.0	20.509758617733187	0.0
27	1.7677854686266693E-4	0.0	0.0	20.881523901785375	0.0
28	1.7677854686266693E-4	0.0	0.0	21.222087772316304	0.0
29	1.7677854686266693E-4	0.0	0.0	21.58253922936928	0.0
30	1.7677854686266693E-4	0.0	0.0	22.0988209754817	0.0
31	1.7677854686266693E-4	0.0	0.0	22.482518811447118	0.0
32	1.7677854686266693E-4	0.0	0.0	22.93074081701741	0.0
33	1.7677854686266693E-4	0.0	0.0	23.31585288135773	0.0
34	2.651678202940004E-4	0.0	0.0	23.667465411067575	0.0
35	2.651678202940004E-4	0.0	0.0	24.034104117260746	0.0
36	2.651678202940004E-4	0.0	0.0	24.39694208469637	0.0
37	2.651678202940004E-4	0.0	0.0	24.749173339320233	0.0
38	2.651678202940004E-4	0.0	0.0	25.12809815452036	0.0
39	3.5355709372533387E-4	0.0	0.0	25.51789485035254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.033238E-4	45.000004	39
CGTTTTT	3200	0.0	41.34375	1
GCTACGA	140	0.0	38.57143	10
AGTAACG	30	1.1399108E-4	37.499996	1
CGAATAT	145	0.0	37.241383	14
CTCGTAA	165	0.0	36.81818	33
GCGATAT	110	0.0	36.81818	9
GCGTAAG	135	0.0	36.666664	1
CGTAAGG	215	0.0	36.627907	2
GACACGA	295	0.0	36.61017	25
TTACGGG	210	0.0	36.42857	3
ATCTCGT	25	0.0021070947	36.0	14
ACACGAC	300	0.0	36.0	26
TACGAAT	150	0.0	36.0	12
CATCGAA	25	0.0021070947	36.0	12
TCGCGAT	25	0.0021070947	36.0	43
ACGACCA	300	0.0	36.0	28
TGCACGG	270	0.0	35.833332	2
AGTACGG	145	0.0	35.68966	2
TACGGCT	800	0.0	35.4375	7
>>END_MODULE