FastQCFastQC Report
Sat 14 Jan 2017
SRR2935729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038902
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC183621.7674429349447782No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113261.0901894500154972No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG72380.6966970898121286No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC69760.6714781567462571No Hit
GAACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT63540.6116072545822416No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC36630.3525837855736152No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTT29080.2799109059372299No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTT27220.2620073885698555No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC21810.2099331794529224No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCT19310.18586931202365575No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCT17500.16844707200486667No Hit
CGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTG17030.16392306492816452No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTC16630.16007284613948186No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC16630.16007284613948186No Hit
CGTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT11960.11512154178161174No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGGATAAT11620.11184885581123148No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT10960.10549599480990507No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT207.032975E-445.023
GCGAACG453.8562575E-1045.01
CCCTACG207.032975E-445.031
CGTTTTT61100.043.6743051
CGTTCGA403.4577897E-739.37514
CGTTAGG1150.039.1304362
ACAACGA3050.039.0983613
TAACGGG2200.038.8636323
TCGATAG356.24771E-638.5714261
CAATCGA356.24771E-638.57142641
TAGTACG356.24771E-638.5714261
GCGTTAG601.546141E-1037.5000041
TAGCCGT301.1398463E-437.50000444
AGCGACT3150.037.14285319
TACGGCT9450.036.6666687
TTGCGAG1050.036.428571
AACGGGA4450.036.404494
TAGGGCG3100.036.290325
TTACACG6100.036.14753734
AACGAGC3300.036.13636415
Produced by FastQC (version 0.11.5)