##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935729.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038902
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.002984882115925	33.0	31.0	34.0	30.0	34.0
2	32.05951668203546	33.0	31.0	34.0	30.0	34.0
3	32.04808923267065	33.0	31.0	34.0	30.0	34.0
4	35.74542930902049	37.0	35.0	37.0	35.0	37.0
5	34.81272535811848	37.0	35.0	37.0	33.0	37.0
6	35.26060975914957	37.0	35.0	37.0	33.0	37.0
7	35.825748723171195	37.0	35.0	37.0	35.0	37.0
8	35.77688848418811	37.0	35.0	37.0	35.0	37.0
9	37.65614466042033	39.0	38.0	39.0	35.0	39.0
10	37.33915518499339	39.0	37.0	39.0	34.0	39.0
11	37.26228171665855	39.0	37.0	39.0	34.0	39.0
12	37.27825049908461	39.0	37.0	39.0	34.0	39.0
13	37.24101888339805	39.0	37.0	39.0	34.0	39.0
14	38.39721359666263	40.0	38.0	41.0	34.0	41.0
15	38.438602486086275	40.0	38.0	41.0	34.0	41.0
16	38.47551357105868	40.0	38.0	41.0	34.0	41.0
17	38.41173180916005	40.0	38.0	41.0	34.0	41.0
18	38.30616843552135	40.0	38.0	41.0	34.0	41.0
19	38.15883211313483	40.0	37.0	41.0	34.0	41.0
20	38.01606888811457	40.0	36.0	41.0	34.0	41.0
21	38.00818364003535	40.0	36.0	41.0	34.0	41.0
22	38.05952149480894	40.0	36.0	41.0	34.0	41.0
23	38.04503600917122	40.0	36.0	41.0	34.0	41.0
24	37.99233421439173	40.0	36.0	41.0	34.0	41.0
25	37.86035160197978	40.0	36.0	41.0	33.0	41.0
26	37.84481981938624	40.0	36.0	41.0	33.0	41.0
27	37.85350398786411	40.0	36.0	41.0	34.0	41.0
28	37.705886599506016	40.0	36.0	41.0	33.0	41.0
29	37.65823340411319	40.0	36.0	41.0	33.0	41.0
30	37.5022437149991	40.0	36.0	41.0	33.0	41.0
31	37.38342692573506	40.0	35.0	41.0	33.0	41.0
32	37.170225873085236	40.0	35.0	41.0	32.0	41.0
33	36.83563127224704	40.0	35.0	41.0	31.0	41.0
34	36.647796423531766	40.0	35.0	41.0	30.0	41.0
35	36.50169217115763	40.0	35.0	41.0	30.0	41.0
36	36.41056326775769	40.0	35.0	41.0	30.0	41.0
37	36.397368567968876	40.0	35.0	41.0	30.0	41.0
38	36.25001106937902	39.0	35.0	41.0	29.0	41.0
39	36.16519267457373	39.0	35.0	41.0	29.0	41.0
40	35.994507662897945	39.0	35.0	41.0	28.0	41.0
41	35.917373342240175	39.0	35.0	41.0	27.0	41.0
42	35.96130626372844	39.0	35.0	41.0	28.0	41.0
43	35.91352986133437	39.0	35.0	41.0	28.0	41.0
44	35.83578720610799	39.0	35.0	41.0	27.0	41.0
45	35.78808877064439	39.0	35.0	41.0	27.0	41.0
46	35.653464908143405	39.0	35.0	41.0	26.0	41.0
47	35.48188472059925	39.0	35.0	41.0	26.0	41.0
48	35.45460880814552	39.0	35.0	41.0	26.0	41.0
49	35.45652525454759	39.0	35.0	40.0	26.0	41.0
50	35.360905070930656	38.0	35.0	40.0	26.0	41.0
51	34.25163104893436	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	19.0
10	18.0
11	16.0
12	20.0
13	14.0
14	15.0
15	26.0
16	54.0
17	126.0
18	337.0
19	665.0
20	1178.0
21	2143.0
22	3220.0
23	4692.0
24	6634.0
25	10311.0
26	14288.0
27	15559.0
28	15561.0
29	15616.0
30	17230.0
31	20638.0
32	26169.0
33	35278.0
34	55743.0
35	69708.0
36	74739.0
37	109109.0
38	194188.0
39	345318.0
40	266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38137957189417	16.373151654342756	19.587410554604766	21.658058219158306
2	31.905993058055525	27.800504763683197	23.991002038690848	16.30250013957043
3	28.58902957160541	27.202469530331065	29.517798598905383	14.69070229915815
4	24.798970451495904	23.922949421600883	34.00137837832634	17.276701748576865
5	25.417989377246364	28.6081844100791	28.165120482971446	17.80870572970309
6	23.66671736121405	34.012447757343814	28.45619702339586	13.864637858046283
7	73.8655811616495	5.184512109900645	16.502904027521364	4.44700270092848
8	74.45119943940814	6.847806626611558	13.730072711381824	4.9709212225984745
9	68.27698858987662	6.705541042369732	15.213176988782386	9.80429337897126
10	34.53059095083078	29.27898877853734	21.91842926474297	14.271991005888909
11	24.967513778970492	23.45543660518509	32.76747951202327	18.809570103821148
12	22.303451143611237	20.74160989198211	35.87797501593028	21.076963948476372
13	22.140683144319677	22.309130216324544	38.078663820071576	17.471522819284203
14	18.993899328329334	27.128160307709486	34.383897614981976	19.494042748979208
15	17.578173879730716	25.0138126599044	38.4189269055214	18.989086554843478
16	20.70753545570227	26.215947221200846	32.35656491180111	20.719952411295772
17	20.97926464671355	25.76720422137988	31.855555191923777	21.39797593998279
18	22.11623425501154	24.404323025655934	32.210834130649474	21.26860858868305
19	20.682701544515268	26.642936484865753	30.329328464089972	22.345033506529006
20	21.89022641211587	26.67518206722097	32.30939973163975	19.12519178902341
21	21.837574670180633	27.564005074588366	32.5448406105677	18.05357964466331
22	20.931233167324734	22.79088884225846	33.27310949444702	23.00476849596978
23	19.77337612209814	26.199102514000362	33.24663924027483	20.780882123626675
24	21.128075602896136	24.515498093179144	32.76507312528034	21.591353178644376
25	20.19035481691247	28.894063155138788	30.086090892114942	20.829491135833795
26	19.981769214035587	25.139618558824605	31.081949981807718	23.79666224533209
27	21.783767862608794	24.510107786874986	30.233361760782056	23.47276258973416
28	19.670864046849463	25.261092961607545	31.73523585477745	23.332807136765545
29	21.319335221223945	23.172349268747197	29.76113242635013	25.74718308367873
30	21.044910877060587	24.63081214590019	30.391798263936348	23.932478713102874
31	24.431852089995015	23.455244094245657	28.587874505968802	23.52502930979053
32	25.289969602522667	25.494223709262283	28.03998837233926	21.175818315875798
33	22.541009642872957	26.66757788511332	27.85999064396834	22.93142182804538
34	21.512038671597512	26.055104331303625	30.874615700037154	21.558241297061706
35	20.562767229247804	28.285343564648063	28.885207651924823	22.266681554179314
36	21.384596429692117	30.22585383414412	27.512989675638316	20.87656006052544
37	21.289784792020807	27.408841257404454	30.078582965477015	21.222790985097728
38	21.659405795734344	28.6955843765822	28.028245205033777	21.616764622649683
39	23.006404838954975	26.265518788105137	29.36070967232713	21.36736670061276
40	21.55843380800114	27.466402028295256	30.589121976856337	20.386042186847266
41	19.324633122277174	28.05962448816154	29.86335573518965	22.752386654371634
42	20.85365125873278	26.910719201618633	30.872786846112533	21.36284269353606
43	22.34897998078741	25.87058259585601	29.02246795174136	22.757969471615226
44	22.112191525283425	24.8299647127448	29.194476476125757	23.863367285846017
45	20.839212938275217	24.114112784458978	29.129407778597017	25.917266498668788
46	23.284486890967578	25.297477529160595	28.45735208903246	22.96068349083937
47	19.98764079768833	24.751420249455673	32.58632671801575	22.674612234840243
48	20.568927579309694	25.589227857873027	30.006295107719495	23.835549455097784
49	20.776069350140823	23.833528090233727	32.493440189738784	22.89696236988667
50	20.399902974486526	24.310858964560662	31.939586216986783	23.349651843966033
51	19.719569314526296	23.801089996939076	29.990509210685897	26.488831477848727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	316.0
1	650.5
2	985.0
3	9782.5
4	18580.0
5	12282.5
6	5985.0
7	5794.0
8	5603.0
9	5532.5
10	5462.0
11	5509.0
12	5556.0
13	5424.5
14	5293.0
15	5130.5
16	4968.0
17	4747.0
18	4526.0
19	4510.5
20	4495.0
21	4579.5
22	4664.0
23	5275.0
24	5886.0
25	6838.0
26	9029.5
27	10269.0
28	11494.0
29	12719.0
30	15482.5
31	18246.0
32	21018.5
33	23791.0
34	26256.5
35	28722.0
36	31980.5
37	35239.0
38	37603.0
39	39967.0
40	42996.0
41	46025.0
42	50382.0
43	54739.0
44	60296.5
45	65854.0
46	97653.5
47	129453.0
48	112271.0
49	95089.0
50	93661.0
51	92233.0
52	79965.0
53	67697.0
54	60763.0
55	53829.0
56	49018.0
57	44207.0
58	39862.0
59	35517.0
60	32225.5
61	28934.0
62	25086.0
63	21238.0
64	19314.0
65	17390.0
66	15288.0
67	13186.0
68	10925.0
69	8664.0
70	7188.0
71	5712.0
72	4992.5
73	4273.0
74	3486.0
75	2058.0
76	1417.0
77	1164.5
78	912.0
79	659.5
80	407.0
81	311.5
82	216.0
83	145.5
84	75.0
85	59.0
86	43.0
87	28.5
88	14.0
89	12.0
90	10.0
91	7.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1038902.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.51725490097809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.46307291804149	26.447239415401985
2	8.608962976054888	6.115334649072488
3	3.5222448086607736	3.753014000785545
4	2.044397331463948	2.9044552456193773
5	1.4446877629381338	2.565567176429875
6	1.1305754771100927	2.409296244318845
7	0.9645119205236533	2.397977101538936
8	0.843172600222226	2.3957740934090634
9	0.7113379668542182	2.2738294700554293
>10	6.174281708323588	38.2814219846805
>50	0.06282549377212072	1.4813297434924908
>100	0.02426678597732606	1.6175752912026535
>500	0.0010785238204784526	0.2806887204254221
>1k	0.0032355714614353583	2.263705210991514
>5k	8.088928653588396E-4	1.969705084172665
>10k+	5.392619102392263E-4	2.8430865684032516
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC	18362	1.7674429349447782	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11326	1.0901894500154972	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG	7238	0.6966970898121286	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	6976	0.6714781567462571	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT	6354	0.6116072545822416	No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	3663	0.3525837855736152	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTT	2908	0.2799109059372299	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTT	2722	0.2620073885698555	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC	2181	0.2099331794529224	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCT	1931	0.18586931202365575	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCT	1750	0.16844707200486667	No Hit
CGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTG	1703	0.16392306492816452	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTC	1663	0.16007284613948186	No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	1663	0.16007284613948186	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT	1196	0.11512154178161174	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGGATAAT	1162	0.11184885581123148	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	1096	0.10549599480990507	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8127734858533335E-4	0.0	0.0	0.11069379017462667	0.0
2	4.8127734858533335E-4	0.0	0.0	0.7046862937986451	0.0
3	4.8127734858533335E-4	0.0	0.0	1.1252264409925095	0.0
4	4.8127734858533335E-4	0.0	0.0	2.1321549097027437	0.0
5	4.8127734858533335E-4	0.0	0.0	4.8452115791479855	0.0
6	4.8127734858533335E-4	0.0	0.0	6.460955893818666	0.0
7	4.8127734858533335E-4	0.0	0.0	7.562792255669929	0.0
8	4.8127734858533335E-4	0.0	0.0	8.991897214559218	0.0
9	4.8127734858533335E-4	0.0	0.0	9.70226258107117	0.0
10	4.8127734858533335E-4	0.0	0.0	11.378551586193886	0.0
11	4.8127734858533335E-4	0.0	0.0	12.218380559475293	0.0
12	4.8127734858533335E-4	0.0	0.0	13.764243403131383	0.0
13	4.8127734858533335E-4	0.0	0.0	14.274878669980422	0.0
14	4.8127734858533335E-4	0.0	0.0	14.55613715249369	0.0
15	4.8127734858533335E-4	0.0	0.0	14.894667639488613	0.0
16	4.8127734858533335E-4	0.0	0.0	15.319250516410595	0.0
17	4.8127734858533335E-4	0.0	0.0	15.802164207981118	0.0
18	4.8127734858533335E-4	0.0	0.0	16.388648784967206	0.0
19	5.775328183024001E-4	0.0	0.0	16.870118644491974	0.0
20	5.775328183024001E-4	0.0	0.0	17.248402640480045	0.0
21	5.775328183024001E-4	0.0	0.0	17.63217319824199	0.0
22	6.737882880194667E-4	0.0	0.0	18.09843469355146	0.0
23	6.737882880194667E-4	0.0	0.0	18.50963806018277	0.0
24	6.737882880194667E-4	0.0	0.0	18.844222072919294	0.0
25	6.737882880194667E-4	0.0	0.0	19.158496181545516	0.0
26	7.700437577365333E-4	0.0	0.0	19.481529537915993	0.0
27	7.700437577365333E-4	0.0	0.0	19.843931381400747	0.0
28	7.700437577365333E-4	0.0	0.0	20.17370262065142	0.0
29	8.662992274536001E-4	0.0	0.0	20.514061961570967	0.0
30	8.662992274536001E-4	0.0	0.0	21.04288951219653	0.0
31	8.662992274536001E-4	0.0	0.0	21.443601032628678	0.0
32	9.625546971706667E-4	0.0	0.0	21.841136122560165	0.0
33	0.0010588101668877333	0.0	0.0	22.216147432577856	0.0
34	0.0010588101668877333	0.0	0.0	22.583073283139314	0.0
35	0.0010588101668877333	0.0	0.0	22.96848018388645	0.0
36	0.0011550656366048002	0.0	0.0	23.321256480399498	0.0
37	0.0012513211063218668	0.0	0.0	23.690781228643317	0.0
38	0.0012513211063218668	0.0	0.0	24.093225347530375	0.0
39	0.0012513211063218668	0.0	0.0	24.60193550498507	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	20	7.032975E-4	45.0	23
GCGAACG	45	3.8562575E-10	45.0	1
CCCTACG	20	7.032975E-4	45.0	31
CGTTTTT	6110	0.0	43.674305	1
CGTTCGA	40	3.4577897E-7	39.375	14
CGTTAGG	115	0.0	39.130436	2
ACAACGA	305	0.0	39.09836	13
TAACGGG	220	0.0	38.863632	3
TCGATAG	35	6.24771E-6	38.571426	1
CAATCGA	35	6.24771E-6	38.571426	41
TAGTACG	35	6.24771E-6	38.571426	1
GCGTTAG	60	1.546141E-10	37.500004	1
TAGCCGT	30	1.1398463E-4	37.500004	44
AGCGACT	315	0.0	37.142853	19
TACGGCT	945	0.0	36.666668	7
TTGCGAG	105	0.0	36.42857	1
AACGGGA	445	0.0	36.40449	4
TAGGGCG	310	0.0	36.29032	5
TTACACG	610	0.0	36.147537	34
AACGAGC	330	0.0	36.136364	15
>>END_MODULE