FastQCFastQC Report
Sat 14 Jan 2017
SRR2935728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200179
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120891.0072664160929328No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC93740.7810501600177974TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT43170.35969634529516015No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC37780.31478637769865997No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG36880.30728749628180463TruSeq Adapter, Index 27 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT28500.23746457820041844TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC27700.23079890582988036TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG26440.22030047184628293No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT26160.21796748651659462No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC25820.21513457575911593No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC24690.20571931353573092TruSeq Adapter, Index 16 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT17280.14397852320362212No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT16930.14106229154151173TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT16300.135813074549713TruSeq Adapter, Index 27 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG253.8909096E-545.01
TATAGCG302.1655833E-644.9999961
CGTTTTT55200.042.758151
CGGTCTA900.042.531
ATTAGCG403.45839E-739.3751
TAATGCG403.45839E-739.3751
TCACGAC1000.038.2525
CGTTAGG1250.037.8000032
TTACGCG301.139952E-437.4999961
ATACGAT301.139952E-437.49999611
ACGGGTA1150.037.1739165
CGTTCTG5100.037.0588231
CTATCGG250.002107145736.02
ATAGCGG1650.035.4545482
TTCGCAA702.1827873E-1135.35714315
GTTTTTT67400.035.3523752
CGTTTTC4350.035.1724171
TAACGGG2650.034.811323
CGTTTCT6450.034.534881
GGGCGAT20750.034.265067
Produced by FastQC (version 0.11.5)