##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935728.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1200179
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.044105920866805	33.0	31.0	34.0	30.0	34.0
2	32.09977011762412	33.0	31.0	34.0	30.0	34.0
3	32.089027553389954	33.0	31.0	34.0	30.0	34.0
4	35.78145926565954	37.0	35.0	37.0	35.0	37.0
5	34.89381417271924	37.0	35.0	37.0	33.0	37.0
6	35.342728043066906	37.0	35.0	37.0	33.0	37.0
7	35.884879672115574	37.0	35.0	37.0	35.0	37.0
8	35.8650326326323	37.0	35.0	37.0	35.0	37.0
9	37.68844064093773	39.0	38.0	39.0	35.0	39.0
10	37.41861422337835	39.0	37.0	39.0	34.0	39.0
11	37.351154286152315	39.0	37.0	39.0	34.0	39.0
12	37.268349971129304	39.0	37.0	39.0	34.0	39.0
13	37.212917406486866	39.0	37.0	39.0	34.0	39.0
14	38.29496266806868	40.0	38.0	41.0	33.0	41.0
15	38.391738232380334	40.0	38.0	41.0	34.0	41.0
16	38.407281747139386	40.0	38.0	41.0	34.0	41.0
17	38.387224738976435	40.0	38.0	41.0	34.0	41.0
18	38.310237056305766	40.0	37.0	41.0	34.0	41.0
19	38.1818920344382	40.0	37.0	41.0	34.0	41.0
20	38.11018106465786	40.0	36.0	41.0	34.0	41.0
21	38.017419901531355	40.0	36.0	41.0	34.0	41.0
22	38.04495329446691	40.0	36.0	41.0	34.0	41.0
23	38.00213718120381	40.0	36.0	41.0	34.0	41.0
24	37.92198247094808	40.0	36.0	41.0	34.0	41.0
25	37.675353426447224	40.0	35.0	41.0	33.0	41.0
26	37.523389427743695	39.0	35.0	41.0	33.0	41.0
27	37.43845626360734	39.0	35.0	41.0	33.0	41.0
28	37.16986299543652	39.0	35.0	41.0	32.0	41.0
29	37.023786451854264	39.0	35.0	41.0	32.0	41.0
30	36.84145448303961	39.0	35.0	41.0	31.0	41.0
31	36.64798917494807	39.0	35.0	41.0	30.0	41.0
32	36.59737339180239	39.0	35.0	41.0	30.0	41.0
33	36.36137942756872	39.0	35.0	41.0	30.0	41.0
34	36.313713204447005	39.0	35.0	41.0	30.0	41.0
35	36.25345219338115	39.0	35.0	41.0	30.0	41.0
36	36.2660328167715	39.0	35.0	41.0	30.0	41.0
37	36.284473399384595	39.0	35.0	41.0	30.0	41.0
38	36.225693834003096	39.0	35.0	41.0	30.0	41.0
39	36.16748168398214	39.0	35.0	41.0	30.0	41.0
40	36.05758057756385	39.0	35.0	41.0	30.0	41.0
41	35.926878407304244	39.0	35.0	41.0	29.0	41.0
42	35.934602255163604	39.0	35.0	41.0	29.0	41.0
43	35.83239916712424	38.0	35.0	41.0	29.0	41.0
44	35.71330276567079	38.0	35.0	40.0	28.0	41.0
45	35.67526593949736	38.0	35.0	40.0	28.0	41.0
46	35.5609346605798	38.0	35.0	40.0	28.0	41.0
47	35.467094491738315	38.0	35.0	40.0	28.0	41.0
48	35.40451049385133	38.0	35.0	40.0	27.0	41.0
49	35.389858512771845	38.0	35.0	40.0	27.0	41.0
50	35.25130001441452	38.0	34.0	40.0	27.0	41.0
51	33.974817922993154	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	18.0
10	20.0
11	28.0
12	28.0
13	23.0
14	34.0
15	55.0
16	80.0
17	160.0
18	319.0
19	566.0
20	1086.0
21	1733.0
22	2708.0
23	4232.0
24	6324.0
25	9677.0
26	13407.0
27	16194.0
28	17455.0
29	18829.0
30	21862.0
31	26583.0
32	33663.0
33	46397.0
34	76839.0
35	91797.0
36	99497.0
37	147609.0
38	243052.0
39	319635.0
40	261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27800936360326	16.717589626214092	19.96410535428465	22.040295655897996
2	32.6326322990154	25.13291767311376	24.92203246349086	17.31241756437998
3	29.879209684555384	24.63949127588468	29.32704204956094	16.15425698999899
4	27.236020626923153	23.8109482002268	30.737248360452902	18.21578281239715
5	23.996753817555547	29.13790359604692	27.71536579126947	19.149976795128058
6	24.841627790521247	32.18278273490871	27.796270389666876	15.179319084903167
7	75.11996127244352	5.505845378064439	13.886261965923415	5.487931383568618
8	75.56097882065926	5.4785161213452325	13.062051577306386	5.89845348068913
9	68.45320573014526	6.6600065490231035	15.89062964774421	8.99615807308743
10	37.77953121992636	23.829445441055043	22.050544127167697	16.3404792118509
11	28.593984730611016	23.471748797471044	28.852196214064733	19.082070257853204
12	25.20332383752757	20.483277911044937	33.1783842243532	21.135014027074295
13	24.31745597948306	20.61192538779632	35.73600271292865	19.334615919791965
14	19.97685345269331	24.503428238621073	34.160821010865874	21.35889729781974
15	18.73870480986586	23.154212829919537	37.63255314415599	20.474529216058603
16	22.32783609778208	24.528257868201326	32.254938638319786	20.88896739569681
17	22.12020040343982	24.478515288136187	30.740831159352062	22.66045314907193
18	23.85294193616119	23.53907208841348	30.919387857977853	21.68859811744748
19	22.476730554358976	26.17859502624192	28.73538030577106	22.60929411362805
20	23.000485760874003	26.04594814606821	28.675555896245474	22.27801019681231
21	22.61745956228196	26.965394328679302	30.6768407045949	19.740305404443838
22	21.909981761053977	23.21911981462765	30.827901504692218	24.042996919626155
23	20.375127376832953	25.474533382103836	30.79340665017468	23.356932590888526
24	21.777584843594163	23.253114743717397	31.083863323720877	23.885437088967564
25	22.948993441811595	25.55043872622334	28.500165391995697	23.000402439969374
26	21.020531104110304	23.98475560728858	29.641828427259597	25.35288486134152
27	22.701946959578528	23.04947845279746	29.480852439511107	24.767722148112906
28	21.139013430496618	23.933763213653965	30.372719402689096	24.55450395316032
29	21.595778629687736	23.267695902027945	30.29598084952328	24.840544618761033
30	22.459899731623366	21.92372971031821	31.073781494260437	24.542589063797983
31	22.234600005499182	22.02513125125502	30.42004567651992	25.320223066725877
32	22.571383102020615	21.93947736129361	30.73099929260552	24.758140244080256
33	21.275076467760226	21.280825610179814	30.221325318973253	27.22277260308671
34	19.797046940498042	21.697513454243076	33.1925487781406	25.312890827118288
35	19.432101378211083	22.066208457238464	31.71393600454599	26.787754160004468
36	20.303054794326513	22.527972910707486	31.87666173129175	25.292310563674253
37	19.799213284018467	22.629791056167456	30.47170463739159	27.099291022422488
38	19.91711236407236	22.313671543994687	32.183615943955026	25.585600147977928
39	20.97103848675906	21.52103977823308	29.27604965592632	28.231872079081537
40	22.662202888069196	20.899715792394304	32.70037219448099	23.737709125055513
41	20.116832572474603	22.63545687768241	30.103342918014732	27.144367631828253
42	21.23724877705742	22.759605025583685	31.59528703635041	24.40785916100848
43	21.94780945175678	21.499126380314937	31.064532873846318	25.488531294081966
44	22.510725483448716	21.995135725587602	30.13092213744783	25.363216653515853
45	21.073773162169974	21.26032866764041	30.08334590090311	27.5825522692865
46	22.8867527260517	21.817578877817393	29.256135959719344	26.039532436411566
47	20.386209057148974	22.11728417177771	32.15237060471813	25.344136166355185
48	20.63400542752373	22.160694363090837	31.377236228929185	25.82806398045625
49	21.60111116758417	21.018531402399145	31.58745487131503	25.79290255870166
50	20.074172269303162	20.94654214079733	33.394518650967896	25.584766938931608
51	20.29072330044102	20.580263444036266	30.95738219048992	28.1716310650328
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	411.0
1	860.5
2	1310.0
3	10147.5
4	18985.0
5	12138.5
6	5292.0
7	5216.5
8	5141.0
9	5061.5
10	4982.0
11	4875.0
12	4768.0
13	4631.5
14	4495.0
15	4373.0
16	4251.0
17	4064.5
18	3878.0
19	3907.0
20	3936.0
21	3927.0
22	3918.0
23	4213.0
24	4508.0
25	5315.5
26	6832.5
27	7542.0
28	9184.0
29	10826.0
30	13010.5
31	15195.0
32	17814.5
33	20434.0
34	22372.0
35	24310.0
36	27495.5
37	30681.0
38	33560.5
39	36440.0
40	39089.5
41	41739.0
42	46531.0
43	51323.0
44	59603.0
45	67883.0
46	93928.0
47	119973.0
48	121162.0
49	122351.0
50	121183.5
51	120016.0
52	104481.0
53	88946.0
54	79182.0
55	69418.0
56	64272.5
57	59127.0
58	55652.0
59	52177.0
60	49745.0
61	47313.0
62	43862.5
63	40412.0
64	36313.5
65	32215.0
66	27339.0
67	22463.0
68	19221.0
69	15979.0
70	14186.5
71	12394.0
72	10293.0
73	8192.0
74	6656.0
75	4066.5
76	3013.0
77	2250.5
78	1488.0
79	1099.0
80	710.0
81	480.0
82	250.0
83	180.0
84	110.0
85	78.0
86	46.0
87	50.0
88	54.0
89	36.5
90	19.0
91	10.5
92	2.0
93	9.5
94	17.0
95	8.5
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1200179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.42446860687948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65416342038962	24.284268044039045
2	9.127832588588781	6.101859076122744
3	3.6119437472298004	3.621819011672913
4	2.1544047502927715	2.880393357706911
5	1.5169692015543885	2.5351944727478846
6	1.178172115948688	2.362786614181662
7	0.9714517267874877	2.272918042157507
8	0.8345005000872853	2.2314188614074157
9	0.7285249253652999	2.1915502647481517
>10	7.105376837744831	42.549926792780504
>50	0.07701309799248052	1.757086929069972
>100	0.03354753608311647	1.965517990958617
>500	0.0022873319757672848	0.5487616095348247
>1k	0.003303923964997189	2.8732682168585
>5k	2.541479973074761E-4	0.7962989671485211
>10k+	2.541479973074761E-4	1.0269317488647824
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12089	1.0072664160929328	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	9374	0.7810501600177974	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT	4317	0.35969634529516015	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	3778	0.31478637769865997	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	3688	0.30728749628180463	TruSeq Adapter, Index 27 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	2850	0.23746457820041844	TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2770	0.23079890582988036	TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	2644	0.22030047184628293	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT	2616	0.21796748651659462	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	2582	0.21513457575911593	No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2469	0.20571931353573092	TruSeq Adapter, Index 16 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT	1728	0.14397852320362212	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	1693	0.14106229154151173	TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	1630	0.135813074549713	TruSeq Adapter, Index 27 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6664180926345153E-4	0.0	0.0	0.14781128481668152	0.0
2	1.6664180926345153E-4	0.0	0.0	0.690313694873848	0.0
3	1.6664180926345153E-4	0.0	0.0	1.3364673102928812	0.0
4	1.6664180926345153E-4	0.0	0.0	2.110185230703087	0.0
5	1.6664180926345153E-4	0.0	0.0	4.261114383771088	0.0
6	1.6664180926345153E-4	0.0	0.0	6.488282164577117	0.0
7	1.6664180926345153E-4	0.0	0.0	7.957729638662233	0.0
8	1.6664180926345153E-4	0.0	0.0	10.050917404820447	0.0
9	1.6664180926345153E-4	0.0	0.0	11.27781772552261	0.0
10	2.499627138951773E-4	0.0	0.0	13.10087911886477	0.0
11	2.499627138951773E-4	0.0	0.0	14.374105862542171	0.0
12	2.499627138951773E-4	0.0	0.0	15.75439996867134	0.0
13	2.499627138951773E-4	0.0	0.0	16.44096422283676	0.0
14	2.499627138951773E-4	0.0	0.0	16.879148860295007	0.0
15	2.499627138951773E-4	0.0	0.0	17.256175953753566	0.0
16	3.3328361852690306E-4	0.0	0.0	17.761600561249615	0.0
17	3.3328361852690306E-4	0.0	0.0	18.279856588058948	0.0
18	3.3328361852690306E-4	0.0	0.0	18.942674384404327	0.0
19	3.3328361852690306E-4	0.0	0.0	19.366444505361283	0.0
20	3.3328361852690306E-4	0.0	0.0	19.805962277293638	0.0
21	3.3328361852690306E-4	0.0	0.0	20.240480794948088	0.0
22	3.3328361852690306E-4	0.0	0.0	20.717826257583244	0.0
23	3.3328361852690306E-4	0.0	0.0	21.1675925007853	0.0
24	4.999254277903546E-4	0.0	0.0	21.56028392431462	0.0
25	4.999254277903546E-4	0.0	0.0	21.91989694870515	0.0
26	4.999254277903546E-4	0.0	0.0	22.271927770774194	0.0
27	4.999254277903546E-4	0.0	0.0	22.67820050175849	0.0
28	4.999254277903546E-4	0.0	0.0	23.031647779206267	0.0
29	4.999254277903546E-4	0.0	0.0	23.4275053971116	0.0
30	4.999254277903546E-4	0.0	0.0	24.0095019159642	0.0
31	6.665672370538061E-4	0.0	0.0	24.414858116997546	0.0
32	8.332090463172577E-4	0.0	0.0	24.87837230946384	0.0
33	8.332090463172577E-4	0.0	0.0	25.306558438366277	0.0
34	8.332090463172577E-4	0.0	0.0	25.743326620445785	0.0
35	8.332090463172577E-4	0.0	0.0	26.161180957173887	0.0
36	9.165299509489834E-4	0.0	0.0	26.564954061019233	0.0
37	9.998508555807092E-4	0.0	0.0	26.972226642859106	0.0
38	0.001083171760212435	0.0	0.0	27.450072030922055	0.0
39	0.001083171760212435	0.0	0.0	28.10080829609583	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTAG	25	3.8909096E-5	45.0	1
TATAGCG	30	2.1655833E-6	44.999996	1
CGTTTTT	5520	0.0	42.75815	1
CGGTCTA	90	0.0	42.5	31
ATTAGCG	40	3.45839E-7	39.375	1
TAATGCG	40	3.45839E-7	39.375	1
TCACGAC	100	0.0	38.25	25
CGTTAGG	125	0.0	37.800003	2
TTACGCG	30	1.139952E-4	37.499996	1
ATACGAT	30	1.139952E-4	37.499996	11
ACGGGTA	115	0.0	37.173916	5
CGTTCTG	510	0.0	37.058823	1
CTATCGG	25	0.0021071457	36.0	2
ATAGCGG	165	0.0	35.454548	2
TTCGCAA	70	2.1827873E-11	35.357143	15
GTTTTTT	6740	0.0	35.352375	2
CGTTTTC	435	0.0	35.172417	1
TAACGGG	265	0.0	34.81132	3
CGTTTCT	645	0.0	34.53488	1
GGGCGAT	2075	0.0	34.26506	7
>>END_MODULE