##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935727.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660106
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10667226172766	33.0	31.0	34.0	30.0	34.0
2	32.18822280058051	33.0	31.0	34.0	30.0	34.0
3	32.15190742092937	33.0	31.0	34.0	30.0	34.0
4	35.82752315537201	37.0	35.0	37.0	35.0	37.0
5	34.87009662084574	37.0	35.0	37.0	33.0	37.0
6	35.34030443595422	37.0	35.0	37.0	33.0	37.0
7	35.88952683356916	37.0	35.0	37.0	35.0	37.0
8	35.86336739856932	37.0	35.0	37.0	35.0	37.0
9	37.730267563088354	39.0	38.0	39.0	35.0	39.0
10	37.43816144679794	39.0	37.0	39.0	35.0	39.0
11	37.374182328292726	39.0	37.0	39.0	34.0	39.0
12	37.30582209523925	39.0	37.0	39.0	34.0	39.0
13	37.252726077326976	39.0	37.0	39.0	34.0	39.0
14	38.40328674485613	40.0	38.0	41.0	34.0	41.0
15	38.456775123995236	40.0	38.0	41.0	34.0	41.0
16	38.4873883891375	40.0	38.0	41.0	34.0	41.0
17	38.44926420908157	40.0	38.0	41.0	34.0	41.0
18	38.33397969417033	40.0	38.0	41.0	34.0	41.0
19	38.14783080293165	40.0	37.0	41.0	34.0	41.0
20	37.9566281778987	40.0	36.0	41.0	34.0	41.0
21	37.924350028631764	40.0	35.0	41.0	34.0	41.0
22	37.98875786616089	40.0	35.0	41.0	34.0	41.0
23	37.97346486776366	40.0	35.0	41.0	34.0	41.0
24	37.93644657070228	40.0	35.0	41.0	34.0	41.0
25	37.796047907457286	40.0	35.0	41.0	34.0	41.0
26	37.786404001781534	40.0	35.0	41.0	34.0	41.0
27	37.78082610974601	40.0	35.0	41.0	34.0	41.0
28	37.661283187851645	40.0	35.0	41.0	33.0	41.0
29	37.59291538025711	40.0	35.0	41.0	33.0	41.0
30	37.42282148624615	40.0	35.0	41.0	33.0	41.0
31	37.26181098187261	40.0	35.0	41.0	33.0	41.0
32	37.01404319912257	40.0	35.0	41.0	32.0	41.0
33	36.64639012522231	40.0	35.0	41.0	31.0	41.0
34	36.36457478041405	40.0	35.0	41.0	30.0	41.0
35	36.19253574425925	39.0	35.0	41.0	29.0	41.0
36	36.041456978121694	39.0	35.0	41.0	28.0	41.0
37	35.93782362226673	39.0	35.0	41.0	28.0	41.0
38	35.79478295910051	39.0	35.0	41.0	27.0	41.0
39	35.71281885030586	39.0	35.0	41.0	26.0	41.0
40	35.56309441210957	39.0	35.0	41.0	25.0	41.0
41	35.45819762280603	39.0	35.0	41.0	24.0	41.0
42	35.455126903860894	39.0	35.0	41.0	25.0	41.0
43	35.36674867369786	39.0	35.0	41.0	24.0	41.0
44	35.27870675315783	39.0	35.0	41.0	24.0	41.0
45	35.24719666235423	38.0	35.0	41.0	24.0	41.0
46	35.131628859607396	38.0	35.0	41.0	23.0	41.0
47	35.01220713036997	38.0	35.0	40.0	23.0	41.0
48	34.95835062853542	38.0	35.0	40.0	23.0	41.0
49	34.931800347216964	38.0	34.0	40.0	24.0	41.0
50	34.810256231574925	38.0	34.0	40.0	23.0	41.0
51	33.6851429921861	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	4.0
10	15.0
11	13.0
12	11.0
13	11.0
14	21.0
15	34.0
16	53.0
17	104.0
18	221.0
19	450.0
20	846.0
21	1408.0
22	2174.0
23	3147.0
24	4605.0
25	7487.0
26	10703.0
27	11633.0
28	11255.0
29	10602.0
30	11149.0
31	13050.0
32	16399.0
33	22542.0
34	38779.0
35	47184.0
36	46827.0
37	67158.0
38	119090.0
39	212959.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.97221658339721	16.507045838092672	20.39475478180777	23.12598279670235
2	33.71897846709468	25.971889363223482	24.470312343775092	15.838819825906747
3	28.576319560797813	25.24382447667496	31.387383238449583	14.79247272407765
4	25.09157620139796	23.463958818735172	34.64746571005263	16.796999269814243
5	24.214292855995858	28.68493847957752	29.806728010349854	17.29404065407677
6	23.667865464031536	32.785795008680424	29.66493260173366	13.88140692555438
7	74.47425110512555	4.85725019921043	16.458417284496733	4.210081411167297
8	76.26017639591217	5.422765434642315	14.050773663623723	4.266284505821792
9	70.71652128597529	5.496389973731492	15.887599870323857	7.899488869969369
10	36.877865070155394	24.48500695342869	23.17218749715955	15.464940479256361
11	28.156689986153737	23.517132096966247	30.295588890269137	18.030589026610876
12	24.785564742632243	20.47428746292262	34.53187821349905	20.20826958094609
13	24.070679557525608	20.700766240573483	36.93346220152521	18.2950920003757
14	20.154641830251503	25.14353755305966	35.0964845040039	19.60533611268493
15	19.240849196947156	23.436387489282023	37.64107582721563	19.681687486555187
16	22.872235671240677	24.022808458035527	32.86078296516014	20.24417290556365
17	22.96100929244697	24.50712461331968	31.657188390955394	20.874677703277957
18	24.02326293049904	23.169157680736124	32.358590892977794	20.44898849578704
19	22.746043817205116	25.782222855117208	30.11486034061196	21.356872987065714
20	23.39851478398924	25.556501531572202	31.411773260658137	19.633210423780422
21	23.12219552617307	26.11065495541625	32.49856841173993	18.268581106670748
22	21.86845749016067	21.982378587681374	33.93909463025635	22.210069291901604
23	21.259009916589154	24.229744919755312	33.26405153111773	21.247193632537805
24	22.09205794220928	23.871772109327896	32.835938470488074	21.20023147797475
25	21.608347750209813	26.49119989819817	30.68461731903664	21.21583503255538
26	20.81938355355049	23.615904112369833	32.23982208917961	23.32489024490006
27	22.08418041950838	23.480471318242827	32.01182840331705	22.423519858931748
28	20.41066131803074	24.662705686662445	32.623548339206124	22.303084656100687
29	21.609408185958014	23.524858128845974	33.705041311546935	21.16069237364908
30	21.56714224685126	24.182782765192258	34.14300127555271	20.107073712403768
31	22.17658982042278	24.826467264348455	31.739296416030154	21.257646499198614
32	25.296088809979008	25.062338472911925	30.12364680823989	19.51792590886918
33	23.718160416660357	25.536807724819955	29.479053364156666	21.26597849436303
34	21.69075875692692	25.25867057714973	31.184840010543763	21.86573065537959
35	23.835717293889164	25.07127643136102	30.22317627774933	20.869829997000483
36	24.520758787225084	27.240776481352995	28.181988953289324	20.056475778132604
37	23.556671201291916	26.077629956400944	29.565403132224215	20.800295710082924
38	23.674531060163066	26.903860895068366	28.104274162028524	21.317333882740044
39	24.24959021732873	25.119450512493447	27.747361787349305	22.883597482828517
40	24.441983560216087	24.375327598900785	30.213026392730864	20.969662448152267
41	22.33126194883852	25.0162852632759	29.407852678206226	23.244600109679357
42	22.87344759781005	25.112633425540746	30.157732243003398	21.856186733645806
43	23.263081989862233	24.368056039484568	29.917013328162444	22.451848642490752
44	23.147191511666307	23.891617406901318	29.489203249175134	23.471987832257245
45	21.72408673758457	24.11749022126749	29.153651080281044	25.00477196086689
46	23.41366386610635	24.557874038412013	29.03776060208512	22.990701493396514
47	21.022078272277483	24.642708898267855	31.97956085840759	22.355651971047074
48	21.552599128018834	24.427591932204827	30.185606554098886	23.834202385677454
49	22.321415045462395	23.526524527878856	31.44267738817705	22.709383038481697
50	21.054800289650448	23.502437487312644	32.135141931750354	23.30762029128655
51	20.62199101356449	23.30459047486313	30.775208830096982	25.2982096814754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	425.0
2	678.0
3	8153.5
4	15629.0
5	10279.5
6	4930.0
7	4746.0
8	4562.0
9	4520.0
10	4478.0
11	4471.5
12	4465.0
13	4258.0
14	4051.0
15	3900.5
16	3750.0
17	3561.5
18	3373.0
19	3225.5
20	3078.0
21	3063.5
22	3049.0
23	3238.0
24	3427.0
25	3696.0
26	4408.0
27	4851.0
28	6045.0
29	7239.0
30	7959.0
31	8679.0
32	10416.5
33	12154.0
34	13777.5
35	15401.0
36	16751.0
37	18101.0
38	19921.5
39	21742.0
40	23604.0
41	25466.0
42	27740.0
43	30014.0
44	33210.0
45	36406.0
46	51624.0
47	66842.0
48	62895.5
49	58949.0
50	58184.5
51	57420.0
52	51391.0
53	45362.0
54	42102.5
55	38843.0
56	36395.0
57	33947.0
58	32579.0
59	31211.0
60	27935.5
61	24660.0
62	21920.5
63	19181.0
64	16989.5
65	14798.0
66	12546.0
67	10294.0
68	8463.0
69	6632.0
70	5819.5
71	5007.0
72	4074.5
73	3142.0
74	2567.5
75	1512.0
76	1031.0
77	812.0
78	593.0
79	439.0
80	285.0
81	213.5
82	142.0
83	102.0
84	62.0
85	44.0
86	26.0
87	20.0
88	14.0
89	10.0
90	6.0
91	5.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	660106.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.66617586125521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.67591711315035	28.827757090882283
2	8.637949418358783	6.852688414184933
3	3.95566873616678	4.707187552129819
4	2.6602197056672754	4.220829706982989
5	1.9803286543927596	3.9276032334113045
6	1.5940108834925946	3.7936989617623227
7	1.3671857965491607	3.7961722568650504
8	1.1311700620530936	3.5895352488307983
9	0.9606778106170922	3.429577348374888
>10	4.977254503204418	29.317980081174916
>50	0.036155017973471176	1.0008405624021526
>100	0.016538997214647477	1.1024502714154247
>500	0.0038462784220110297	1.0731573774060452
>1k	0.0023077670532066176	1.7423169249327606
>5k	7.692556844022059E-4	2.6182049692444047
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9639	1.460220025268669	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC	7522	1.1395139568493544	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCT	2519	0.38160537853011484	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG	2433	0.3685771679093964	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC	2368	0.35873026453327195	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	1553	0.23526524527878856	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTT	1339	0.20284620954816346	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTT	1208	0.18300091197474344	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTG	983	0.14891547721123577	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC	925	0.14012900958330934	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGACGGT	874	0.13240297770358095	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCT	713	0.10801295549502657	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCT	678	0.10271077675403648	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5149082117114524E-4	0.0	0.0	0.08256249753827416	0.0
2	1.5149082117114524E-4	0.0	0.0	0.47765055915262095	0.0
3	1.5149082117114524E-4	0.0	0.0	0.8130512372255365	0.0
4	3.029816423422905E-4	0.0	0.0	1.5099090146128047	0.0
5	3.029816423422905E-4	0.0	0.0	3.385819853175096	0.0
6	4.5447246351343573E-4	0.0	0.0	4.717272680448292	0.0
7	4.5447246351343573E-4	0.0	0.0	5.610159580431022	0.0
8	4.5447246351343573E-4	0.0	0.0	6.843143373942973	0.0
9	4.5447246351343573E-4	0.0	0.0	7.57166273295501	0.0
10	4.5447246351343573E-4	0.0	0.0	8.888269459753433	0.0
11	4.5447246351343573E-4	0.0	0.0	9.647389964642043	0.0
12	4.5447246351343573E-4	0.0	0.0	10.700251171781503	0.0
13	4.5447246351343573E-4	0.0	0.0	11.15639003432782	0.0
14	4.5447246351343573E-4	0.0	0.0	11.442253213877772	0.0
15	4.5447246351343573E-4	0.0	0.0	11.685395981857459	0.0
16	4.5447246351343573E-4	0.0	0.0	11.988832096663263	0.0
17	4.5447246351343573E-4	0.0	0.0	12.317718669425819	0.0
18	4.5447246351343573E-4	0.0	0.0	12.71613952910593	0.0
19	4.5447246351343573E-4	0.0	0.0	13.145010043841443	0.0
20	4.5447246351343573E-4	0.0	0.0	13.440114163482834	0.0
21	4.5447246351343573E-4	0.0	0.0	13.724159453178732	0.0
22	4.5447246351343573E-4	0.0	0.0	14.073497286799393	0.0
23	4.5447246351343573E-4	0.0	0.0	14.396324226715103	0.0
24	4.5447246351343573E-4	0.0	0.0	14.660221237195238	0.0
25	4.5447246351343573E-4	0.0	0.0	14.909575128842944	0.0
26	4.5447246351343573E-4	0.0	0.0	15.161352873629387	0.0
27	4.5447246351343573E-4	0.0	0.0	15.482361923691043	0.0
28	4.5447246351343573E-4	0.0	0.0	15.783980148642794	0.0
29	4.5447246351343573E-4	0.0	0.0	16.101807891459856	0.0
30	4.5447246351343573E-4	0.0	0.0	16.56794514820347	0.0
31	6.05963284684581E-4	0.0	0.0	16.912435275546656	0.0
32	6.05963284684581E-4	0.0	0.0	17.245866572944344	0.0
33	6.05963284684581E-4	0.0	0.0	17.588538810433477	0.0
34	6.05963284684581E-4	0.0	0.0	17.917728364838375	0.0
35	6.05963284684581E-4	0.0	0.0	18.27691310183516	0.0
36	6.05963284684581E-4	0.0	0.0	18.62503900888645	0.0
37	6.05963284684581E-4	0.0	0.0	18.97407386086477	0.0
38	6.05963284684581E-4	0.0	0.0	19.395066852899383	0.0
39	7.574541058557262E-4	0.0	0.0	19.97421626223667	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	20	7.031102E-4	45.0	1
GATCACG	20	7.031102E-4	45.0	1
CGTTCAT	20	7.031102E-4	45.0	17
TCGTAAG	40	6.8066583E-9	45.0	1
TATAGCG	20	7.031102E-4	45.0	1
AATTGCG	30	2.1640935E-6	44.999996	1
CGTTTTT	5150	0.0	43.907764	1
CGTAAGG	115	0.0	43.04348	2
CTCACGA	65	0.0	41.538464	24
CGGTCTA	65	0.0	41.538464	31
GTACGGT	55	6.002665E-11	40.909092	32
GCGTAAG	50	1.0804797E-9	40.500004	1
ACGGGCT	195	0.0	40.384617	5
CTAGTTG	40	3.455243E-7	39.375	1
CGACGAA	35	6.2442487E-6	38.571426	19
TACGGTT	60	1.546141E-10	37.499996	33
AACGGGC	120	0.0	37.499996	4
TAACACG	30	1.13939575E-4	37.499996	1
TTGCACG	30	1.13939575E-4	37.499996	1
CGCCCCG	30	1.13939575E-4	37.499996	25
>>END_MODULE