##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935726.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481621
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8960365100359	33.0	31.0	34.0	30.0	34.0
2	31.87834832783454	33.0	31.0	34.0	30.0	34.0
3	31.884334362496652	33.0	31.0	34.0	30.0	34.0
4	35.579021678871975	37.0	35.0	37.0	33.0	37.0
5	34.65425303298652	37.0	35.0	37.0	33.0	37.0
6	35.15412118657617	37.0	35.0	37.0	32.0	37.0
7	35.66207661210786	37.0	35.0	37.0	33.0	37.0
8	35.727551331856375	37.0	35.0	37.0	35.0	37.0
9	37.44807016305352	39.0	37.0	39.0	35.0	39.0
10	37.099933350082324	39.0	37.0	39.0	33.0	39.0
11	37.142167804144755	39.0	37.0	39.0	34.0	39.0
12	36.96853127251511	39.0	35.0	39.0	33.0	39.0
13	36.84105136611568	39.0	35.0	39.0	33.0	39.0
14	37.89650575867746	40.0	36.0	41.0	33.0	41.0
15	38.04167177095683	40.0	37.0	41.0	33.0	41.0
16	38.083277099628134	40.0	36.0	41.0	34.0	41.0
17	38.010364996542926	40.0	36.0	41.0	33.0	41.0
18	37.94986929556643	40.0	36.0	41.0	33.0	41.0
19	37.973821739500565	40.0	36.0	41.0	34.0	41.0
20	37.9289773494096	40.0	36.0	41.0	34.0	41.0
21	37.8320982681403	40.0	36.0	41.0	33.0	41.0
22	37.79787841477012	40.0	36.0	41.0	33.0	41.0
23	37.87539995141408	40.0	36.0	41.0	34.0	41.0
24	37.89798409953055	40.0	36.0	41.0	34.0	41.0
25	37.61643699091194	39.0	35.0	41.0	33.0	41.0
26	37.74740304098036	40.0	35.0	41.0	33.0	41.0
27	37.67687663121002	40.0	36.0	41.0	33.0	41.0
28	37.17629007040806	39.0	35.0	41.0	32.0	41.0
29	36.80877702591872	39.0	35.0	41.0	31.0	41.0
30	36.77047512463119	39.0	35.0	41.0	31.0	41.0
31	36.79913251290953	39.0	35.0	41.0	31.0	41.0
32	36.91582800583861	39.0	35.0	41.0	31.0	41.0
33	36.740885468033994	39.0	35.0	41.0	31.0	41.0
34	36.621293506720015	39.0	35.0	41.0	31.0	41.0
35	36.62569946077932	39.0	35.0	41.0	31.0	41.0
36	36.507428039890286	39.0	35.0	41.0	30.0	41.0
37	36.464421194258556	39.0	35.0	41.0	30.0	41.0
38	36.279950417444425	39.0	35.0	41.0	30.0	41.0
39	36.23733599656161	39.0	35.0	41.0	30.0	41.0
40	36.182622850747784	39.0	35.0	41.0	30.0	41.0
41	36.0042232377741	38.0	35.0	41.0	29.0	41.0
42	36.00755365733637	38.0	35.0	40.0	29.0	41.0
43	35.68758422078771	38.0	35.0	40.0	28.0	41.0
44	35.57416308674248	38.0	35.0	40.0	27.0	41.0
45	35.615845239306424	38.0	35.0	40.0	28.0	41.0
46	35.406336102454006	38.0	34.0	40.0	27.0	41.0
47	35.45100608154545	38.0	34.0	40.0	27.0	41.0
48	35.377610195568714	38.0	34.0	40.0	28.0	41.0
49	35.026896667711746	37.0	34.0	40.0	26.0	41.0
50	34.72257438940578	37.0	34.0	40.0	24.0	41.0
51	33.509454529599	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	9.0
10	10.0
11	12.0
12	9.0
13	14.0
14	10.0
15	14.0
16	29.0
17	44.0
18	126.0
19	279.0
20	527.0
21	971.0
22	1512.0
23	2254.0
24	3059.0
25	4044.0
26	4877.0
27	5792.0
28	6800.0
29	7736.0
30	9333.0
31	11134.0
32	14169.0
33	19102.0
34	32941.0
35	45518.0
36	35666.0
37	52775.0
38	89352.0
39	133417.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25297692584003	18.927123194378982	23.12419931855131	16.695700561229682
2	27.778689052180034	25.77379308626493	27.45768976020564	18.989828101349403
3	30.50676776967782	24.569941925289804	28.519520535857033	16.403769769175348
4	27.75294266653655	25.538545869054712	27.48509720298741	19.223414261421325
5	24.165474512116376	31.18261039281925	25.861413850309685	18.790501244754694
6	24.8788985530116	34.68681805818268	24.95011637781575	15.48416701098997
7	78.85266630815518	6.371815182477508	9.80023711590649	4.9752813934608335
8	80.03056345134453	5.54668504903233	8.518731533716345	5.9040199659068024
9	73.4399039908974	8.332070237801092	11.406894632916755	6.821131138384747
10	39.255555717047216	29.655268354162295	17.52685202680116	13.562323901989323
11	29.046906177263864	28.4225563254094	24.731272099846144	17.79926539748059
12	26.63754279817533	20.17561526594563	33.740846017926955	19.445995917952082
13	27.460596610197648	24.56142900745607	30.33983152727975	17.638142855066537
14	18.396623070837858	32.15910435799103	29.711951929006418	19.732320642164687
15	16.54122224736878	24.30438041530581	40.110169614697035	19.044227722628374
16	19.64054723527421	26.495937677136173	31.2943164853692	22.569198602220418
17	19.780076034890506	22.384821259870314	27.524339677879496	30.310763027359688
18	25.66354041871098	21.498024380166147	29.481895515353358	23.356539685769516
19	26.819428554817996	24.21800544411477	26.51711615564936	22.44544984541787
20	24.760340599766206	23.74128204542576	26.15043779237201	25.34793956243602
21	25.739326150645425	26.744888615737274	27.49381775296343	20.021967480653874
22	20.49578402935088	26.91722329383478	26.449220445121785	26.13777223169256
23	19.342595111093576	25.04209741684852	26.63006804105303	28.985239431004878
24	20.832770996281308	22.625466912779967	27.80671939138866	28.73504269955006
25	19.250406439918525	26.15334464236402	26.74654967287556	27.849699244841897
26	19.053778801173536	25.232496091324926	26.683221869478285	29.030503238023257
27	21.601217554882364	25.539376397623858	25.500341554874062	27.359064492619716
28	22.3115271136433	25.923703492995532	29.330739315769037	22.434030077592134
29	21.701088615322007	24.85294453522583	30.23393913471381	23.212027714738355
30	26.199854242236114	24.61769731801562	26.917430925977065	22.265017513771202
31	23.80606327381904	30.180162409861694	23.742112573994905	22.27166174232436
32	24.499139364770222	31.630472923730483	23.111949022156423	20.758438689342864
33	25.024448684754198	25.45403958714425	26.23141432786361	23.290097400237947
34	24.682270914266613	25.737665093507132	26.499052159270462	23.081011832955788
35	22.27747544230837	28.371271186264718	26.50943376638477	22.841819605042136
36	25.42268713365904	25.423517662228186	27.87108535549737	21.282709848615404
37	22.986746840357874	29.916884853442856	26.861577879702093	20.234790426497185
38	21.7716835436993	29.179583116184716	23.102813207895835	25.945920132220145
39	26.42908012732003	23.859839998671152	25.97644205713621	23.734637816872603
40	25.88902892523374	23.203930061189194	27.57645534559332	23.330585667983748
41	22.28079755658495	23.466584721181178	25.56263119755991	28.689986524673966
42	26.55905784839116	24.50744465046167	26.021913496296882	22.911584004850287
43	25.279213323339306	26.326717481172956	25.50117208344321	22.892897112044533
44	21.775420922260448	24.868932210181864	26.234944074282474	27.120702793275207
45	20.15630547671302	24.595688310933287	26.24138067069335	29.006625541660352
46	22.980102611804718	26.035824849830053	25.98910761781567	24.994964920549563
47	21.238068938023883	23.068346272276333	29.124560598478887	26.569024191220898
48	21.662884301141354	23.19666293620918	28.026394197927416	27.114058564722054
49	24.293375911764645	21.760471408015846	30.259270256072725	23.686882424146788
50	22.011706300182095	21.675342229678524	31.521881313314825	24.791070156824556
51	22.881269712076506	22.517290566648878	26.735960433618967	27.86547928765565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	221.0
1	464.0
2	707.0
3	1319.0
4	1931.0
5	1339.0
6	747.0
7	761.0
8	775.0
9	825.0
10	875.0
11	910.5
12	946.0
13	962.5
14	979.0
15	913.0
16	847.0
17	1020.0
18	1193.0
19	1070.5
20	948.0
21	923.0
22	898.0
23	1226.5
24	1555.0
25	1837.0
26	2454.0
27	2789.0
28	3156.5
29	3524.0
30	4006.5
31	4489.0
32	5433.0
33	6377.0
34	7082.5
35	7788.0
36	9099.0
37	10410.0
38	11736.0
39	13062.0
40	15536.0
41	18010.0
42	21901.5
43	25793.0
44	31612.5
45	37432.0
46	43628.5
47	49825.0
48	52676.0
49	55527.0
50	54034.0
51	52541.0
52	44394.0
53	36247.0
54	31805.0
55	27363.0
56	26709.5
57	26056.0
58	25340.5
59	24625.0
60	22413.5
61	20202.0
62	17841.0
63	15480.0
64	12851.0
65	10222.0
66	8531.5
67	6841.0
68	5452.5
69	4064.0
70	3574.5
71	3085.0
72	2629.5
73	2174.0
74	1785.0
75	968.5
76	541.0
77	572.0
78	603.0
79	412.0
80	221.0
81	148.5
82	76.0
83	73.0
84	70.0
85	56.0
86	42.0
87	21.0
88	0.0
89	1.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	481621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.8515352515539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83769151219637	27.94735362158174
2	10.644925893397366	7.845637236214237
3	3.8019195427043684	4.2031971615462504
4	1.827424436341	2.693735841414855
5	1.0627671119646858	1.9582299845379372
6	0.6767111571378724	1.4962707037431682
7	0.5021099532640377	1.2952465850006039
8	0.41514364317871744	1.2238944480847214
9	0.3304328918250167	1.0959263425226247
>10	4.659299888358757	41.12393616058473
>50	0.19904334117553796	4.514087562886606
>100	0.03742695304155411	2.356620778976539
>500	0.002268300184336613	0.63507764009131
>1k	0.0028353752304207665	1.6107859328146814
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	2129	0.44204883092722286	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	2010	0.4173406059951704	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	1342	0.27864233494801927	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	1202	0.24957383502795766	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1025	0.21282294584330833	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	985	0.20451766015186215	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	807	0.16755913882492665	No Hit
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	725	0.150533303157462	No Hit
ACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	522	0.10838397827337262	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1526428457230896E-4	0.0	0.0	0.29712159561148704	0.0
2	4.1526428457230896E-4	0.0	0.0	1.487891931622583	0.0
3	4.1526428457230896E-4	0.0	0.0	2.0194302158751385	0.0
4	4.1526428457230896E-4	0.0	0.0	2.6946499425897126	0.0
5	4.1526428457230896E-4	0.0	0.0	4.435437823516832	0.0
6	4.1526428457230896E-4	0.0	0.0	5.724418162829279	0.0
7	4.1526428457230896E-4	0.0	0.0	6.789571052757251	0.0
8	4.1526428457230896E-4	0.0	0.0	8.607182826330247	0.0
9	6.228964268584634E-4	0.0	0.0	9.31437790295689	0.0
10	6.228964268584634E-4	0.0	0.0	10.905878273580264	0.0
11	6.228964268584634E-4	0.0	0.0	12.497793908488209	0.0
12	6.228964268584634E-4	0.0	0.0	14.327240714171516	0.0
13	6.228964268584634E-4	0.0	0.0	15.397169143372071	0.0
14	6.228964268584634E-4	0.0	0.0	15.91874108479489	0.0
15	6.228964268584634E-4	0.0	0.0	16.391104208495893	0.0
16	6.228964268584634E-4	0.0	0.0	17.2577607703983	0.0
17	6.228964268584634E-4	0.0	0.0	18.323951821037703	0.0
18	6.228964268584634E-4	0.0	0.0	19.91046902024621	0.0
19	6.228964268584634E-4	0.0	0.0	20.62036331472257	0.0
20	0.0010381607114307724	0.0	0.0	21.42929814106943	0.0
21	0.0010381607114307724	0.0	0.0	22.194630217536197	0.0
22	0.0010381607114307724	0.0	0.0	22.952072272596087	0.0
23	0.0010381607114307724	0.0	0.0	23.679822931309058	0.0
24	0.0010381607114307724	0.0	0.0	24.21322990484219	0.0
25	0.0010381607114307724	0.0	0.0	24.740200281964448	0.0
26	0.0010381607114307724	0.0	0.0	25.192215455721406	0.0
27	0.0010381607114307724	0.0	0.0	25.719808729270525	0.0
28	0.0010381607114307724	0.0	0.0	26.199438977951544	0.0
29	0.0010381607114307724	0.0	0.0	26.73616806576125	0.0
30	0.0010381607114307724	0.0	0.0	27.387094831828346	0.0
31	0.0010381607114307724	0.0	0.0	27.929637619622067	0.0
32	0.0010381607114307724	0.0	0.0	28.442904275353442	0.0
33	0.0010381607114307724	0.0	0.0	29.027596388031252	0.0
34	0.0010381607114307724	0.0	0.0	29.677484993386916	0.0
35	0.0010381607114307724	0.0	0.0	30.266537381052736	0.0
36	0.0010381607114307724	0.0	0.0	30.732048644058295	0.0
37	0.0012457928537169267	0.0	0.0	31.207110985609017	0.0
38	0.0012457928537169267	0.0	0.0	31.731589777023842	0.0
39	0.0012457928537169267	0.0	0.0	32.40826292873442	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGC	20	7.0291985E-4	45.000004	13
CGAACCT	20	7.0291985E-4	45.000004	38
CGGCAAA	20	7.0291985E-4	45.000004	10
GTAGCTA	20	7.0291985E-4	45.000004	18
CTAACGG	45	3.8380676E-10	45.000004	2
ACCTAGT	20	7.0291985E-4	45.000004	38
TGCGAAG	35	1.2099554E-7	45.000004	1
AACGCGC	20	7.0291985E-4	45.000004	36
TTGCGTG	20	7.0291985E-4	45.000004	1
CTTTGCG	45	3.8380676E-10	45.000004	1
CATCGGC	20	7.0291985E-4	45.000004	19
ACGTAAG	20	7.0291985E-4	45.000004	1
GCTAACG	20	7.0291985E-4	45.000004	1
GCTCGAA	20	7.0291985E-4	45.000004	30
GTAACGG	20	7.0291985E-4	45.000004	2
GCGTAAG	40	6.8012014E-9	45.000004	1
ACGCGCC	20	7.0291985E-4	45.000004	37
ACGGGCA	50	2.1827873E-11	45.0	5
CGACGGT	30	2.1628657E-6	44.999996	28
TTACTAG	30	2.1628657E-6	44.999996	1
>>END_MODULE