##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935723.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	909566
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00393924135247	33.0	31.0	34.0	30.0	34.0
2	32.027608771655935	33.0	31.0	34.0	30.0	34.0
3	32.06097303549165	33.0	31.0	34.0	30.0	34.0
4	35.733244206577645	37.0	35.0	37.0	33.0	37.0
5	34.82426124107541	37.0	35.0	37.0	33.0	37.0
6	35.285211848288085	37.0	35.0	37.0	33.0	37.0
7	35.86500594789163	37.0	35.0	37.0	35.0	37.0
8	35.82138624354912	37.0	35.0	37.0	35.0	37.0
9	37.67102772091305	39.0	38.0	39.0	35.0	39.0
10	37.33462222642447	39.0	37.0	39.0	34.0	39.0
11	37.2686654954121	39.0	37.0	39.0	34.0	39.0
12	37.3404689709158	39.0	37.0	39.0	34.0	39.0
13	37.32349823981987	39.0	37.0	39.0	34.0	39.0
14	38.537256229894254	40.0	38.0	41.0	34.0	41.0
15	38.542692888696365	40.0	38.0	41.0	34.0	41.0
16	38.594081133199786	40.0	38.0	41.0	34.0	41.0
17	38.505490530648686	40.0	38.0	41.0	34.0	41.0
18	38.45521050698905	40.0	38.0	41.0	34.0	41.0
19	38.352780336995885	40.0	37.0	41.0	34.0	41.0
20	38.25703687253041	40.0	37.0	41.0	34.0	41.0
21	38.28464454476091	40.0	37.0	41.0	34.0	41.0
22	38.341823463058205	40.0	37.0	41.0	34.0	41.0
23	38.30905398838567	40.0	37.0	41.0	34.0	41.0
24	38.289604052921945	40.0	37.0	41.0	34.0	41.0
25	38.16815492223764	40.0	37.0	41.0	34.0	41.0
26	38.15210001253345	40.0	37.0	41.0	34.0	41.0
27	38.16549761094852	40.0	37.0	41.0	34.0	41.0
28	38.03124677043777	40.0	37.0	41.0	34.0	41.0
29	37.976174351284016	40.0	37.0	41.0	33.0	41.0
30	37.890662139965656	40.0	37.0	41.0	33.0	41.0
31	37.86791942530834	40.0	37.0	41.0	33.0	41.0
32	37.72282715053113	40.0	37.0	41.0	33.0	41.0
33	37.52804304470484	40.0	37.0	41.0	33.0	41.0
34	37.39744229665577	40.0	37.0	41.0	32.0	41.0
35	37.35028354182104	40.0	37.0	41.0	32.0	41.0
36	37.24711895563379	40.0	37.0	41.0	32.0	41.0
37	37.24094898006302	40.0	37.0	41.0	32.0	41.0
38	37.20573108493501	40.0	37.0	41.0	32.0	41.0
39	37.098551397039905	40.0	36.0	41.0	31.0	41.0
40	37.031711827399	40.0	36.0	41.0	31.0	41.0
41	36.88915262883617	40.0	36.0	41.0	31.0	41.0
42	36.93056358746919	40.0	36.0	41.0	31.0	41.0
43	36.84619807688502	40.0	36.0	41.0	31.0	41.0
44	36.76369829127298	40.0	36.0	41.0	31.0	41.0
45	36.780866918948156	40.0	36.0	41.0	31.0	41.0
46	36.67548918934965	40.0	35.0	41.0	30.0	41.0
47	36.54286989619225	39.0	35.0	41.0	30.0	41.0
48	36.53727052242498	39.0	35.0	41.0	30.0	41.0
49	36.54282262089832	39.0	35.0	41.0	30.0	41.0
50	36.423378842217055	39.0	35.0	41.0	30.0	41.0
51	35.337836946411805	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	15.0
10	18.0
11	13.0
12	8.0
13	7.0
14	12.0
15	14.0
16	35.0
17	91.0
18	181.0
19	433.0
20	835.0
21	1542.0
22	2586.0
23	3537.0
24	4713.0
25	6586.0
26	8149.0
27	9175.0
28	9596.0
29	10223.0
30	12097.0
31	15626.0
32	20483.0
33	27587.0
34	42704.0
35	57755.0
36	62140.0
37	91315.0
38	169086.0
39	352701.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5525646297245	17.16346037560771	21.797868433956413	19.486106560711374
2	28.96315385579496	29.80586345575802	25.22015994441305	16.010822744033966
3	28.004125044251875	28.86200671529059	28.203670761659954	14.93019747879758
4	24.902535934720515	24.104572950176237	33.76632371922433	17.226567395878913
5	26.756167227007165	29.602579691853038	26.157749959870973	17.483503121268825
6	23.755285487804073	35.66799990325056	26.15104346468535	14.425671144260011
7	76.6002686995776	4.5114922941270885	14.449308791225707	4.438930215069605
8	77.61767700199876	7.130983348102282	10.240598263347575	5.010741386551388
9	70.77463317670185	6.768722665534992	12.09752783195502	10.359116325808133
10	32.593456659549716	35.58345408689419	18.340505251955328	13.482584001600765
11	22.697198444093118	25.03842491913726	33.97092679365763	18.29344984311199
12	20.773753636349642	21.945411328039967	35.999806501122514	21.281028534487877
13	21.476286492678927	23.340912039368224	38.70956038374346	16.47324108420939
14	18.759826114872368	29.500003298276322	31.90301748306335	19.837153103787962
15	16.35824118315768	26.336296651369995	39.06511457112513	18.240347594347195
16	19.33163728635415	27.444957265333137	31.58440399047458	21.639001457838134
17	19.412774883845703	26.801903325322186	32.906682967481196	20.87863882335092
18	20.055389053680546	25.58802769672569	32.99199838164576	21.36458486794801
19	19.187062840959314	28.31163433989397	30.61844879865782	21.882854020488892
20	20.54375383424622	27.633728613426623	32.79080352607727	19.031714026249883
21	20.152578262599967	29.65502228535367	32.43513939615157	17.75726005589479
22	19.205863015987845	24.816340980203748	32.53782573227231	23.439970271536094
23	17.803216039297862	28.879047809614693	32.158304070292864	21.159432080794577
24	19.41915155139924	26.106186906722545	31.623653478692038	22.85100806318618
25	18.01991279357408	31.41828080645055	29.5223216347137	21.039484765261673
26	18.340615194499353	27.76467018336217	31.001158794414042	22.893555827724434
27	21.613604730168014	27.319842650230992	29.074085882717693	21.992466736883305
28	17.654903547406125	27.5103730790289	32.93087032716702	21.903853046397952
29	19.87310431568462	26.254499398614286	31.02138822251491	22.85100806318618
30	21.007821312582045	27.446936231125612	30.801723019549982	20.743519436742357
31	20.914590035247578	25.789222552294174	28.74052020414132	24.555667208316933
32	22.098561291868872	27.267619941818406	30.062249468427797	20.571569297884924
33	20.29154563824945	24.69595389449474	29.501432551348667	25.511067915907148
34	19.784600567743297	25.785814333429354	31.95106237480293	22.478522724024426
35	21.906381724910563	24.51377909904284	30.970264939542595	22.609574236504002
36	19.48401765237487	26.45052695461352	31.25402664567497	22.81142874733664
37	20.280111613670694	24.65923308478989	35.05386085231858	20.006794449220838
38	19.82846764280987	24.961025368142607	30.315227262232757	24.895279726814767
39	20.345307542278405	24.47057167924043	34.10032916797682	21.083791610504353
40	21.47430752688645	24.182632156435048	32.71736190666758	21.62569841001093
41	18.724974328415968	25.825393649278887	33.269273477680564	22.18035854462458
42	21.797648548868363	25.163539534239405	32.42007726761994	20.61873464927229
43	21.253982668657358	24.783468159539822	31.577917380377013	22.384631791425804
44	20.738901849893246	24.563802956574893	31.172009507831206	23.525285685700652
45	19.41024620533309	23.714166976338166	31.231268539061485	25.644318279267253
46	23.34179157972044	25.350881629260545	29.565858882148188	21.741467908870824
47	18.7196970863027	24.3393002816728	34.55659072568676	22.384411906337746
48	20.171818207804602	24.771814249873017	30.826130264323865	24.23023727799852
49	19.89762150300253	22.528986351732584	34.534272389249374	23.039119756015506
50	20.124982684049318	22.26457453334887	33.89462666810325	23.715816114498566
51	19.703902740427854	22.541739686839655	31.074380528735684	26.679977043996807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	550.5
2	927.0
3	5114.5
4	9302.0
5	6238.5
6	3175.0
7	3114.5
8	3054.0
9	3056.5
10	3059.0
11	2980.0
12	2901.0
13	2856.0
14	2811.0
15	2804.5
16	2798.0
17	2691.5
18	2585.0
19	2771.0
20	2957.0
21	3524.0
22	4091.0
23	4433.5
24	4776.0
25	6021.5
26	8794.5
27	10322.0
28	12583.0
29	14844.0
30	17568.5
31	20293.0
32	23570.0
33	26847.0
34	30127.0
35	33407.0
36	34870.5
37	36334.0
38	40236.5
39	44139.0
40	46085.5
41	48032.0
42	52289.5
43	56547.0
44	62100.0
45	67653.0
46	98266.0
47	128879.0
48	112375.5
49	95872.0
50	90806.5
51	85741.0
52	71123.5
53	56506.0
54	48584.5
55	40663.0
56	34590.5
57	28518.0
58	24868.0
59	21218.0
60	18145.5
61	15073.0
62	12942.5
63	10812.0
64	9084.0
65	7356.0
66	5910.0
67	4464.0
68	3422.5
69	2381.0
70	1909.0
71	1437.0
72	1255.0
73	1073.0
74	853.0
75	485.5
76	338.0
77	266.5
78	195.0
79	127.5
80	60.0
81	42.0
82	24.0
83	17.5
84	11.0
85	7.0
86	3.0
87	6.0
88	9.0
89	6.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	909566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.41916873156322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50901151939932	25.645382395081928
2	9.294066630992255	6.397880951490273
3	3.4629670769719736	3.575773444024398
4	1.9562881313758649	2.693352451255216
5	1.3767219539259925	2.369281261431307
6	1.0073340274740652	2.0802959916404995
7	0.8641532750403814	2.0820406168483485
8	0.7150616470906558	1.968946198774623
9	0.6340555772777837	1.9641299309561482
>10	6.058357469294032	40.0467355814161
>50	0.08763496557426811	1.927573407548807
>100	0.027927589960389257	1.6914587351591486
>500	0.0012840271246119125	0.32759652163684205
>1k	0.0038520813738357374	2.7277244270389063
>5k	6.420135623059562E-4	1.7049382547649612
>10k+	6.420135623059562E-4	2.796889830932553
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	14854	1.6330865489695086	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	10460	1.1499990105171038	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	9813	1.0788661845319636	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5618	0.6176572123408307	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	4317	0.4746219625623649	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	4052	0.4454871883953446	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	2251	0.24748066660363294	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	2224	0.24451221791491765	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC	1790	0.19679715380742027	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	1676	0.18426370378840018	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	1587	0.17447881737004242	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT	1541	0.16942146034482378	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTA	1353	0.14875226206784334	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	1315	0.14457444539483666	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	1295	0.1423755945143068	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1287	0.14149605416209488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.298276320794753E-4	0.0	0.0	0.15183065330058512	0.0
2	3.298276320794753E-4	0.0	0.0	1.0012467484492604	0.0
3	3.298276320794753E-4	0.0	0.0	1.4897214715589633	0.0
4	3.298276320794753E-4	0.0	0.0	2.4142283242777323	0.0
5	3.298276320794753E-4	0.0	0.0	5.102213583181429	0.0
6	3.298276320794753E-4	0.0	0.0	6.537953265623385	0.0
7	3.298276320794753E-4	0.0	0.0	7.767220850383589	0.0
8	3.298276320794753E-4	0.0	0.0	9.581932482084863	0.0
9	3.298276320794753E-4	0.0	0.0	10.391879203928028	0.0
10	3.298276320794753E-4	0.0	0.0	12.754764360145389	0.0
11	3.298276320794753E-4	0.0	0.0	13.852100892073802	0.0
12	3.298276320794753E-4	0.0	0.0	16.183872308331665	0.0
13	3.298276320794753E-4	0.0	0.0	16.821099293509214	0.0
14	4.39770176105967E-4	0.0	0.0	17.150267270324527	0.0
15	4.39770176105967E-4	0.0	0.0	17.604220034609913	0.0
16	4.39770176105967E-4	0.0	0.0	18.18966408155098	0.0
17	4.39770176105967E-4	0.0	0.0	18.91924280371078	0.0
18	4.39770176105967E-4	0.0	0.0	19.811866318661867	0.0
19	4.39770176105967E-4	0.0	0.0	20.494499574522354	0.0
20	4.39770176105967E-4	0.0	0.0	21.02958993629929	0.0
21	4.39770176105967E-4	0.0	0.0	21.5683084020291	0.0
22	4.39770176105967E-4	0.0	0.0	22.164306933196713	0.0
23	4.39770176105967E-4	0.0	0.0	22.729741437124957	0.0
24	4.39770176105967E-4	0.0	0.0	23.18501351193866	0.0
25	4.39770176105967E-4	0.0	0.0	23.577838221745317	0.0
26	4.39770176105967E-4	0.0	0.0	23.97879867980993	0.0
27	4.39770176105967E-4	0.0	0.0	24.44143690507341	0.0
28	4.39770176105967E-4	0.0	0.0	24.865925067559694	0.0
29	4.39770176105967E-4	0.0	0.0	25.32009771693313	0.0
30	4.39770176105967E-4	0.0	0.0	25.930168893736134	0.0
31	5.497127201324588E-4	0.0	0.0	26.397424705848724	0.0
32	5.497127201324588E-4	0.0	0.0	26.843901377140305	0.0
33	5.497127201324588E-4	0.0	0.0	27.269269079978802	0.0
34	5.497127201324588E-4	0.0	0.0	27.679794539373724	0.0
35	5.497127201324588E-4	0.0	0.0	28.11450735845447	0.0
36	6.596552641589506E-4	0.0	0.0	28.530200117418637	0.0
37	6.596552641589506E-4	0.0	0.0	28.942044887341876	0.0
38	6.596552641589506E-4	0.0	0.0	29.362355233155153	0.0
39	6.596552641589506E-4	0.0	0.0	29.790031729418207	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAGCG	25	3.8901606E-5	45.000004	1
CCACGCG	25	3.8901606E-5	45.000004	1
CCGTTAG	25	3.8901606E-5	45.000004	1
TAGTACG	25	3.8901606E-5	45.000004	1
CCTAGCG	20	7.03251E-4	45.0	1
TCGTACC	20	7.03251E-4	45.0	45
TTGCACG	20	7.03251E-4	45.0	1
GCGTAAG	40	6.8121153E-9	45.0	1
CGGCTAG	30	2.1650012E-6	44.999996	26
CGTTTTT	3150	0.0	43.214287	1
CTGTACG	100	0.0	42.750004	1
TAGGGCG	255	0.0	40.588234	5
CGTATGG	95	0.0	40.263157	2
CTACGAA	45	1.927583E-8	40.0	11
ACGTAAG	40	3.457153E-7	39.375	1
CGTAAGG	120	0.0	39.374996	2
TGCACGG	110	0.0	38.863636	2
ACGGGCG	145	0.0	38.793102	5
GCGAACG	35	6.2468534E-6	38.571426	1
ACGTCGG	35	6.2468534E-6	38.571426	2
>>END_MODULE