##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935722.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920796
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03946693947411	33.0	31.0	34.0	30.0	34.0
2	32.07467017667322	33.0	31.0	34.0	30.0	34.0
3	32.12717040473677	33.0	31.0	34.0	30.0	34.0
4	35.785189118979666	37.0	35.0	37.0	35.0	37.0
5	34.817949904213314	37.0	35.0	37.0	33.0	37.0
6	35.29714942289063	37.0	35.0	37.0	33.0	37.0
7	35.85764925129996	37.0	35.0	37.0	35.0	37.0
8	35.82126985781867	37.0	35.0	37.0	35.0	37.0
9	37.644546674833514	39.0	37.0	39.0	35.0	39.0
10	37.335257755246545	39.0	37.0	39.0	34.0	39.0
11	37.30245895942207	39.0	37.0	39.0	34.0	39.0
12	37.251760433364176	39.0	37.0	39.0	34.0	39.0
13	37.18898648560593	39.0	37.0	39.0	34.0	39.0
14	38.35061946402895	40.0	38.0	41.0	34.0	41.0
15	38.41004304970917	40.0	38.0	41.0	34.0	41.0
16	38.43514632991455	40.0	38.0	41.0	34.0	41.0
17	38.37413933162177	40.0	38.0	41.0	34.0	41.0
18	38.35010251999357	40.0	37.0	41.0	34.0	41.0
19	38.2715900155952	40.0	37.0	41.0	34.0	41.0
20	38.226526831133064	40.0	37.0	41.0	34.0	41.0
21	38.17564259618852	40.0	37.0	41.0	34.0	41.0
22	38.21169292655485	40.0	37.0	41.0	34.0	41.0
23	38.183054661401655	40.0	37.0	41.0	34.0	41.0
24	38.129735576609804	40.0	37.0	41.0	34.0	41.0
25	37.96615645593595	40.0	37.0	41.0	33.0	41.0
26	37.97422013127772	40.0	37.0	41.0	34.0	41.0
27	37.96831654351235	40.0	37.0	41.0	34.0	41.0
28	37.827872840455434	40.0	36.0	41.0	33.0	41.0
29	37.77496970012902	40.0	36.0	41.0	33.0	41.0
30	37.66713039587487	40.0	36.0	41.0	33.0	41.0
31	37.65554911185539	40.0	36.0	41.0	33.0	41.0
32	37.5693486939561	40.0	36.0	41.0	33.0	41.0
33	37.452727857201815	40.0	36.0	41.0	33.0	41.0
34	37.384261008953125	40.0	36.0	41.0	33.0	41.0
35	37.303407052159216	40.0	36.0	41.0	32.0	41.0
36	37.253224384119825	40.0	35.0	41.0	32.0	41.0
37	37.19969895612057	40.0	35.0	41.0	32.0	41.0
38	37.13504511314124	40.0	35.0	41.0	32.0	41.0
39	37.04341569685359	40.0	35.0	41.0	32.0	41.0
40	36.91918731184757	39.0	35.0	41.0	31.0	41.0
41	36.80653152272599	39.0	35.0	41.0	31.0	41.0
42	36.816820446657026	39.0	35.0	41.0	31.0	41.0
43	36.73946563625385	39.0	35.0	41.0	31.0	41.0
44	36.627263802188544	39.0	35.0	41.0	31.0	41.0
45	36.59415766358672	39.0	35.0	41.0	31.0	41.0
46	36.453975690598135	39.0	35.0	41.0	31.0	41.0
47	36.326910629498826	39.0	35.0	41.0	30.0	41.0
48	36.28338958900777	39.0	35.0	41.0	30.0	41.0
49	36.270863470301784	39.0	35.0	40.0	30.0	41.0
50	36.15806758500254	38.0	35.0	40.0	30.0	41.0
51	34.993135287294905	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	18.0
10	25.0
11	19.0
12	26.0
13	21.0
14	21.0
15	37.0
16	65.0
17	107.0
18	225.0
19	420.0
20	811.0
21	1434.0
22	2185.0
23	3068.0
24	3988.0
25	5225.0
26	6469.0
27	7855.0
28	9134.0
29	10535.0
30	13192.0
31	17027.0
32	21883.0
33	31009.0
34	52761.0
35	71019.0
36	69564.0
37	101520.0
38	176907.0
39	314024.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.697626835911535	17.38039696089036	20.8993088588569	18.0226673443412
2	28.586027741215208	27.62685763187503	26.057346035386775	17.729768591522987
3	31.365253541501048	26.357846906372313	26.24739898956989	16.029500562556745
4	28.120126499246307	24.841441535367224	28.649994135508845	18.388437829877628
5	26.214058271321772	30.210491791884415	24.018240739534054	19.557209197259763
6	25.270961211821074	35.91544706970925	24.068197516062188	14.745394202407484
7	77.33080943010178	5.976459498086438	11.597357069318285	5.095374002493495
8	77.7363281334845	7.342560132754704	9.244175691466948	5.676936042293841
9	71.07643821215557	7.308567804377951	12.014061746575788	9.60093223689069
10	38.094865746593165	26.270639750824287	18.494433077467757	17.14006142511479
11	30.225587426530957	24.875108058679665	25.61750919856298	19.281795316226397
12	27.156286517317625	21.12932723426253	29.989704559967677	21.724681688452165
13	24.653560614946198	22.40735189987793	32.34429775976438	20.59478972541149
14	20.67591518642566	27.58091911780677	29.05279779668895	22.690367899078623
15	19.40799047780399	24.656710063901233	35.108427925403674	20.826871532891108
16	22.08263285244506	23.891828374580253	30.854499802344925	23.17103897062976
17	21.58197907028267	24.029643916785044	29.893374862618867	24.495002150313425
18	22.351204827127834	24.644872479897828	29.94876172355223	23.055160969422108
19	24.193741067511155	26.94940030147829	26.255218311113428	22.601640319897133
20	25.119896263667524	25.094809273715352	27.92507786741037	21.860216595206754
21	23.804512617344123	28.061264384293587	27.114366265709233	21.019856732653054
22	22.04679429537053	24.56602765433386	27.780529020543092	25.60664902975252
23	21.873574602843625	27.034978431704744	27.70027237303377	23.391174592417865
24	22.99108597344037	24.31819860207907	28.137828574407358	24.552886850073197
25	22.40919812857571	27.033458008071275	26.237624837640478	24.319719025712537
26	20.683843109657296	25.844595328389786	27.864803930512295	25.606757631440622
27	22.57296947423751	25.47306895338381	27.353507182915653	24.60045438946303
28	20.823830685624177	26.05159014591723	28.923018779403908	24.201560389054688
29	22.136173484680647	24.819395392681983	27.004678560723548	26.039752561913822
30	21.7138215196417	24.619133879817028	29.16682956919882	24.50021503134245
31	22.74792679377408	24.818309375800936	25.839491049048867	26.594272781376116
32	23.05635558799126	27.504137724316784	24.6488907423577	24.790615945334256
33	21.72620211208563	25.075478173232725	25.96785824438855	27.230461470293093
34	21.304067350422894	24.886076829178233	27.305831041837713	26.50402477856116
35	20.3024339810338	25.894552104917917	27.50402912262868	26.2989847914196
36	20.344897241082716	26.19179492526032	26.03247624881081	27.430831584846153
37	20.400609907080398	27.105786732348967	28.99263246147898	23.500970899091655
38	20.197416148636616	27.378811376244034	25.524437551857304	26.899334923262046
39	21.97946124874565	25.18331964952063	26.994252798665503	25.842966303068216
40	21.94199366634955	24.52758265674482	29.169218806337128	24.36120487056851
41	19.278754468959466	25.338077055069746	28.69169718374102	26.691471292229767
42	21.252590150261298	25.79040308602557	27.956789560336926	25.000217203376206
43	21.60967250074935	23.973496844034944	27.913674690159386	26.50315596505632
44	21.904851889017763	24.240113988331835	27.932136977137173	25.92289714551323
45	20.926567882571167	23.44232598751515	28.215478781402176	27.415627348511507
46	23.12542626162581	24.727409762857352	27.23274210574329	24.91442186977354
47	20.474024648239133	24.29593525601762	30.36861584976477	24.86142424597848
48	20.61868209679451	24.292785807062586	28.207007849730015	26.881524246412887
49	20.6464841289493	23.102076898683315	30.209622978379574	26.04181599398781
50	20.532778161503742	22.54071477287043	29.91411778504685	27.01238928057898
51	19.62008957467235	22.99228059200952	28.1645445896811	29.223085243637026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	345.0
1	1016.5
2	1688.0
3	3111.5
4	4535.0
5	3169.5
6	1804.0
7	1740.5
8	1677.0
9	1691.5
10	1706.0
11	1803.5
12	1901.0
13	1893.5
14	1886.0
15	1912.0
16	1938.0
17	1856.5
18	1775.0
19	1954.0
20	2133.0
21	2469.5
22	2806.0
23	3107.0
24	3408.0
25	4207.0
26	5845.5
27	6685.0
28	8567.0
29	10449.0
30	12199.0
31	13949.0
32	15394.0
33	16839.0
34	19320.5
35	21802.0
36	23459.5
37	25117.0
38	26953.0
39	28789.0
40	32743.5
41	36698.0
42	39908.5
43	43119.0
44	49739.0
45	56359.0
46	76620.5
47	96882.0
48	93719.0
49	90556.0
50	89272.5
51	87989.0
52	76547.5
53	65106.0
54	57666.5
55	50227.0
56	47655.5
57	45084.0
58	43505.5
59	41927.0
60	40864.0
61	39801.0
62	36794.0
63	33787.0
64	29199.5
65	24612.0
66	20709.0
67	16806.0
68	14285.5
69	11765.0
70	10229.5
71	8694.0
72	7316.0
73	5938.0
74	5098.0
75	3292.0
76	2326.0
77	1846.5
78	1367.0
79	1019.5
80	672.0
81	511.5
82	351.0
83	231.0
84	111.0
85	96.0
86	81.0
87	52.0
88	23.0
89	15.5
90	8.0
91	8.0
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	920796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.707037496089256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11380329176704	22.45108299161985
2	9.767326227750978	5.998513054241707
3	3.580684346283965	3.298566254490047
4	1.9100457504846422	2.3460738591751142
5	1.2696519705271097	1.9493625332979783
6	0.9232634507789778	1.70104112411033
7	0.7462955480377448	1.6041567763731657
8	0.640320848003945	1.5729885031347848
9	0.5661671217318653	1.5646783532465989
>10	7.2495427061288265	47.365779645077446
>50	0.17939980461409924	3.4539198945330014
>100	0.04672240226468015	2.5545443107751904
>500	0.003566595592723525	0.7677322880400272
>1k	0.002139957355634115	1.3594666036292238
>5k	0.0010699786778170575	2.012093808255546
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	7385	0.8020234666527657	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	5915	0.642378985138945	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	5072	0.5508277620667336	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	3246	0.3525210795876611	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2408	0.26151286495597287	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	2302	0.25000108601688104	TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	1933	0.20992706310626894	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	1342	0.14574346543642674	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	1182	0.12836719533968435	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC	943	0.10241139188267541	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.430084405231995E-4	0.0	0.0	0.1546488038610072	0.0
2	5.430084405231995E-4	0.0	0.0	0.9034574433424993	0.0
3	5.430084405231995E-4	0.0	0.0	1.2693365305670312	0.0
4	5.430084405231995E-4	0.0	0.0	1.8640393746280393	0.0
5	5.430084405231995E-4	0.0	0.0	3.6406543903318433	0.0
6	5.430084405231995E-4	0.0	0.0	4.9098823192107695	0.0
7	5.430084405231995E-4	0.0	0.0	5.943010178150209	0.0
8	5.430084405231995E-4	0.0	0.0	7.663043714351496	0.0
9	5.430084405231995E-4	0.0	0.0	8.449537139605297	0.0
10	5.430084405231995E-4	0.0	0.0	10.3009787184132	0.0
11	5.430084405231995E-4	0.0	0.0	11.439558816502243	0.0
12	5.430084405231995E-4	0.0	0.0	13.190109427060934	0.0
13	5.430084405231995E-4	0.0	0.0	13.740394180687145	0.0
14	5.430084405231995E-4	0.0	0.0	14.009183358746128	0.0
15	5.430084405231995E-4	0.0	0.0	14.41296443511918	0.0
16	5.430084405231995E-4	0.0	0.0	14.975086772748796	0.0
17	5.430084405231995E-4	0.0	0.0	15.641901137711285	0.0
18	6.516101286278393E-4	0.0	0.0	16.435670876068098	0.0
19	7.602118167324793E-4	0.0	0.0	16.929591353568	0.0
20	9.77415192941759E-4	0.0	0.0	17.423511831067902	0.0
21	9.77415192941759E-4	0.0	0.0	17.91526027480571	0.0
22	9.77415192941759E-4	0.0	0.0	18.46684824868918	0.0
23	9.77415192941759E-4	0.0	0.0	18.9917202072989	0.0
24	9.77415192941759E-4	0.0	0.0	19.422977510762426	0.0
25	9.77415192941759E-4	0.0	0.0	19.805472656266968	0.0
26	9.77415192941759E-4	0.0	0.0	20.15582170209254	0.0
27	0.001086016881046399	0.0	0.0	20.55091464341722	0.0
28	0.001194618569151039	0.0	0.0	20.933626992297967	0.0
29	0.001194618569151039	0.0	0.0	21.347616627352856	0.0
30	0.0013032202572556787	0.0	0.0	21.877049856862975	0.0
31	0.0013032202572556787	0.0	0.0	22.295166356065838	0.0
32	0.0013032202572556787	0.0	0.0	22.697752813869737	0.0
33	0.0013032202572556787	0.0	0.0	23.100990881802268	0.0
34	0.0013032202572556787	0.0	0.0	23.48402903574733	0.0
35	0.0013032202572556787	0.0	0.0	23.897041255609277	0.0
36	0.0013032202572556787	0.0	0.0	24.29995351847749	0.0
37	0.0013032202572556787	0.0	0.0	24.7144861619729	0.0
38	0.0013032202572556787	0.0	0.0	25.11631240796007	0.0
39	0.0013032202572556787	0.0	0.0	25.522591323159528	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	7.0325565E-4	45.0	1
CGACGGT	35	1.2115743E-7	45.0	28
CGGGTCA	65	0.0	41.538464	6
CTAGACG	45	1.927583E-8	40.000004	1
TCGATAG	40	3.4572258E-7	39.375	1
CACGGGC	175	0.0	38.571426	4
ACCGCTT	35	6.246937E-6	38.571426	45
ACGGGCT	285	0.0	37.894737	5
ACGATTC	30	1.1397454E-4	37.500004	11
GCCGATA	30	1.1397454E-4	37.500004	9
GTCGTAG	30	1.1397454E-4	37.500004	1
TTCGAAC	30	1.1397454E-4	37.500004	25
GCGTATG	60	1.546141E-10	37.500004	1
ATGGGCG	350	0.0	37.285713	5
AAGGGCG	225	0.0	37.0	5
CGTTTTT	1190	0.0	36.113445	1
CCCGTAC	25	0.002106891	36.000004	33
AACGACT	25	0.002106891	36.000004	33
TAGTGCG	50	4.8770744E-8	36.000004	1
CCAAACG	25	0.002106891	36.000004	1
>>END_MODULE