FastQCFastQC Report
Sat 14 Jan 2017
SRR2935721.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935721.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362891
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC145654.013601880454462No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG120343.3161472728725703No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC119473.2921731318770653No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC37131.0231722473139317No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCT25790.7106817198552734No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT22970.6329724352491519No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTACCAT22820.62883896266372No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTA20030.551956372574685No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTC19740.5439649922428498No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC13460.37091027333276383No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT9910.2730847554775401No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.2661956345018201No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT7230.19923337861782187No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATG6970.1920686928030731No Hit
AATGCCTTGGTTTTTTCTAATGTAGATGATATGGCTGTGATACATTAGGGC6770.1865573960224971No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTACCATCGT5540.1526629208219548No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTAT5360.14770275371943642No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCTACCA4710.12979103918256446No Hit
TCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC4070.1121548894847213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAG253.8855105E-545.0000041
CAACGTA253.8855105E-545.00000445
GCGGCTA351.208864E-745.00000424
CGATGTG253.8855105E-545.00000410
GCGAACC406.7921064E-945.033
GACAATA207.0268987E-445.021
TAGAACG207.0268987E-445.01
TAGCGCA207.0268987E-445.024
TGAACGT207.0268987E-445.038
CATCTCG207.0268987E-445.043
CGACAAT207.0268987E-445.020
CCCGTAC207.0268987E-445.031
ACGGCTA207.0268987E-445.030
AAGTTAG207.0268987E-445.01
AGCGCAT207.0268987E-445.029
CGTAAGG207.0268987E-445.02
ATGCGCG207.0268987E-445.01
GTCCCAA207.0268987E-445.044
ACTCCTA207.0268987E-445.030
CCAACGA207.0268987E-445.044
Produced by FastQC (version 0.11.5)