##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935721.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	362891
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.780837772223617	31.0	31.0	34.0	30.0	34.0
2	31.82472422848734	33.0	31.0	34.0	30.0	34.0
3	31.91962324775208	33.0	31.0	34.0	30.0	34.0
4	35.606110374740624	37.0	35.0	37.0	33.0	37.0
5	34.64858318338013	37.0	35.0	37.0	32.0	37.0
6	35.07506937344822	37.0	35.0	37.0	32.0	37.0
7	35.576087585528434	37.0	35.0	37.0	33.0	37.0
8	35.40032957554748	37.0	35.0	37.0	33.0	37.0
9	37.294146176124514	39.0	37.0	39.0	34.0	39.0
10	37.088715344276935	39.0	37.0	39.0	33.0	39.0
11	36.97789143296472	39.0	37.0	39.0	33.0	39.0
12	36.791008870432165	39.0	35.0	39.0	33.0	39.0
13	36.4856444497108	39.0	35.0	39.0	32.0	39.0
14	37.574999104414275	40.0	36.0	41.0	32.0	41.0
15	37.501404002854855	40.0	36.0	41.0	32.0	41.0
16	37.828604181420864	40.0	36.0	41.0	33.0	41.0
17	37.551826857100345	39.0	36.0	41.0	32.0	41.0
18	37.64308290919312	39.0	36.0	41.0	33.0	41.0
19	37.56038865664897	39.0	36.0	41.0	32.0	41.0
20	37.27116957984629	39.0	35.0	41.0	32.0	41.0
21	37.55164773995497	39.0	35.0	41.0	33.0	41.0
22	37.573692927077275	39.0	35.0	41.0	33.0	41.0
23	37.4615821279668	39.0	35.0	41.0	33.0	41.0
24	37.427301861991616	39.0	35.0	41.0	32.0	41.0
25	37.44508130540576	39.0	35.0	41.0	33.0	41.0
26	37.300892003383936	39.0	35.0	41.0	32.0	41.0
27	37.253031902141416	39.0	35.0	41.0	32.0	41.0
28	37.15615432733245	39.0	35.0	41.0	32.0	41.0
29	37.0611175256482	39.0	35.0	41.0	32.0	41.0
30	37.04170122709023	39.0	35.0	41.0	32.0	41.0
31	37.081746309497895	39.0	35.0	41.0	32.0	41.0
32	36.83191922643439	39.0	35.0	41.0	31.0	41.0
33	36.63700119319575	39.0	35.0	41.0	31.0	41.0
34	36.55143279938053	39.0	35.0	41.0	31.0	41.0
35	36.51075116219471	39.0	35.0	41.0	31.0	41.0
36	36.50015844978244	39.0	35.0	41.0	30.0	41.0
37	36.40434731090052	39.0	35.0	41.0	30.0	41.0
38	36.39309599852297	39.0	35.0	41.0	30.0	41.0
39	36.27367446423306	39.0	35.0	41.0	30.0	41.0
40	36.07375217351767	39.0	35.0	40.0	30.0	41.0
41	36.02191291599957	39.0	35.0	40.0	29.0	41.0
42	36.14888217123048	39.0	35.0	40.0	30.0	41.0
43	35.980652592651786	38.0	35.0	40.0	30.0	41.0
44	35.93173156677901	38.0	35.0	40.0	30.0	41.0
45	35.996503082192724	38.0	35.0	40.0	30.0	41.0
46	35.82846088770457	38.0	35.0	40.0	29.0	41.0
47	35.48707738687374	38.0	35.0	40.0	28.0	41.0
48	35.566238898181545	38.0	35.0	40.0	28.0	41.0
49	35.6727088850371	38.0	35.0	40.0	29.0	41.0
50	35.49201550878914	38.0	35.0	40.0	28.0	41.0
51	34.27451769264049	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	3.0
11	4.0
12	2.0
13	3.0
14	4.0
15	1.0
16	14.0
17	42.0
18	125.0
19	256.0
20	556.0
21	1172.0
22	1841.0
23	2550.0
24	2886.0
25	3077.0
26	3198.0
27	3530.0
28	3939.0
29	4621.0
30	6110.0
31	8243.0
32	11151.0
33	15691.0
34	25865.0
35	36430.0
36	28213.0
37	38357.0
38	63456.0
39	101463.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.703905580463555	15.283652667054296	18.655188472571655	16.3572532799105
2	21.721122871606074	39.190831406675834	23.043282969266254	16.044762752451838
3	25.10423240036264	38.29000994789069	23.208621872683533	13.397135779063134
4	24.036694213965074	20.37333524391622	39.70227974791328	15.887690794205422
5	33.348856819265286	26.094336866993117	22.10278017366096	18.454026140080632
6	21.20113202035873	40.5157471527263	24.278364577793333	14.004756249121636
7	66.01596622677333	5.541884477708183	23.375889729974013	5.066259565544475
8	63.623236729486266	17.27460862903737	12.109145721442527	6.99300892003384
9	59.303206748031776	6.604186932164204	12.36790110529057	21.72470521451345
10	43.62880313923465	20.250708890548403	20.20248504371836	15.91800292649859
11	34.62086411622223	20.913166763573635	25.696972369113592	18.768996751090548
12	25.611822833853694	17.33440619910662	30.88337820447462	26.17039276256507
13	24.233998638709693	22.576751696790495	37.33352439162172	15.855725272878082
14	19.639781642421553	32.63018371907818	26.754590221306124	20.975444417194144
15	13.733876012356328	21.650027143136644	43.87433141080931	20.741765433697722
16	15.676332562670334	23.6685395890226	29.815564453238025	30.839563395069042
17	16.201008016181166	23.889818154762725	33.03140612470411	26.877767704351996
18	17.99989528536117	22.471210363442466	31.93300467633532	27.595889674861045
19	19.462317886087007	25.663353458752074	27.413190186584952	27.461138468575964
20	21.982358339005376	24.749029322854522	33.434833049042275	19.833779289097826
21	19.9368405388946	32.404771680752624	28.151152825504077	19.507234954848702
22	17.18064101892855	22.322129785527885	31.775657153249874	28.72157204229369
23	18.323959536059036	31.011515854623013	27.814137027371856	22.850387581946094
24	21.460989663562888	23.054581127666435	27.368824247501315	28.115604961269362
25	16.155815382580442	34.22019284027436	25.61016944481952	24.013822332325685
26	16.559517871757635	25.126277587484946	30.701780975554644	27.612423565202775
27	23.96918082840302	27.614628083915004	24.621442802384188	23.79474828529779
28	15.723729714983287	25.946358548434656	33.854793863722165	24.475117872859894
29	20.783100159552042	24.716512671849124	29.411310834382775	25.08907633421606
30	21.27498339721845	29.76320713382255	28.176780355533754	20.785029113425242
31	23.62004017735353	22.9250656533229	22.069712392977507	31.385181776346066
32	24.45059260218633	28.88966659410126	24.90775467013511	21.7519861335773
33	21.944330391219403	26.96594845284121	21.489648406821882	29.6000727491175
34	21.830246547861478	26.05934013243646	23.80384192498574	28.30657139471632
35	21.81178370364655	23.081862046730286	24.496336365465112	30.610017884158054
36	19.241314885185908	32.03579036129306	22.96116464723567	25.76173010628536
37	22.68174190046047	21.768520023919027	32.424336784323664	23.125401291296836
38	17.314014401018486	28.40715256096183	22.02782653744513	32.251006500574555
39	20.220121193416205	24.584792678793356	29.852214576828857	25.342871550961583
40	19.088376399524925	26.414267645105554	26.868674064664045	27.628681890705476
41	17.394203769175867	27.814412592210886	22.41113722853419	32.38024641007906
42	19.55352984780554	21.210501224885707	29.716361111187656	29.519607816121095
43	22.98651661242632	27.63667327103731	23.330421531534263	26.046388585002106
44	21.920631815062926	22.2543408351268	29.46118807024699	26.363839279563283
45	18.412140284548254	21.158143905470236	27.872005643567903	32.55771016641361
46	27.707217869828682	25.873333866092025	24.90279450303259	21.516653761046705
47	16.867048232113778	22.707920560168205	36.75731831321251	23.667712894505513
48	21.248804737510714	24.872482370739423	24.80607124453348	29.07264164721638
49	19.553254282966513	18.528979776296463	35.844372001510095	26.07339393922693
50	22.27280367934173	20.391798088131146	29.920830221747025	27.414568010780094
51	19.75744782868685	21.324033938565577	25.066755582254725	33.85176265049285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	131.0
1	402.0
2	673.0
3	1186.0
4	1699.0
5	1173.5
6	648.0
7	659.5
8	671.0
9	685.0
10	699.0
11	749.0
12	799.0
13	796.5
14	794.0
15	785.0
16	776.0
17	774.0
18	772.0
19	759.5
20	747.0
21	872.5
22	998.0
23	1055.5
24	1113.0
25	1289.5
26	1691.0
27	1916.0
28	2181.5
29	2447.0
30	3177.0
31	3907.0
32	4351.5
33	4796.0
34	5688.5
35	6581.0
36	7356.5
37	8132.0
38	8438.0
39	8744.0
40	10653.5
41	12563.0
42	15563.5
43	18564.0
44	21571.5
45	24579.0
46	51971.5
47	79364.0
48	59532.0
49	39700.0
50	37720.0
51	35740.0
52	30243.0
53	24746.0
54	21316.5
55	17887.0
56	15862.5
57	13838.0
58	12574.5
59	11311.0
60	10523.5
61	9736.0
62	8759.5
63	7783.0
64	6976.5
65	6170.0
66	5183.0
67	4196.0
68	3532.5
69	2869.0
70	2347.5
71	1826.0
72	1561.0
73	1296.0
74	1183.0
75	770.5
76	471.0
77	389.0
78	307.0
79	259.5
80	212.0
81	129.0
82	46.0
83	54.0
84	62.0
85	36.5
86	11.0
87	21.0
88	31.0
89	16.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	362891.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.611317442985573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74188195201562	20.81241075929302
2	11.406554926369052	6.527131278583917
3	4.030551700248732	3.4595818249854506
4	1.9441703971490163	2.2250110558434337
5	1.2760664221853286	1.8254970741739645
6	0.8410278518678727	1.443774890891037
7	0.6664329788641418	1.334726785892952
8	0.5247656176580079	1.2011388535982126
9	0.4459509538014209	1.1483319872893183
>10	5.819310651304707	36.622170267114114
>50	0.2454226675653977	4.241949121471529
>100	0.04090377792756628	2.06488526645078
>500	0.0069835718412918045	1.468312110951453
>1k	0.0069835718412918045	4.6224429091655965
>5k	0.0	0.0
>10k+	0.0029929593605536307	11.002635814295237
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC	14565	4.013601880454462	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG	12034	3.3161472728725703	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	11947	3.2921731318770653	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	3713	1.0231722473139317	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCT	2579	0.7106817198552734	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT	2297	0.6329724352491519	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTACCAT	2282	0.62883896266372	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTA	2003	0.551956372574685	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTC	1974	0.5439649922428498	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	1346	0.37091027333276383	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	991	0.2730847554775401	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	966	0.2661956345018201	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT	723	0.19923337861782187	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATG	697	0.1920686928030731	No Hit
AATGCCTTGGTTTTTTCTAATGTAGATGATATGGCTGTGATACATTAGGGC	677	0.1865573960224971	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTACCATCGT	554	0.1526629208219548	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTAT	536	0.14770275371943642	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCTACCA	471	0.12979103918256446	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	407	0.1121548894847213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.266945170863979E-4	0.0	0.0	0.28934308098023925	0.0
2	8.266945170863979E-4	0.0	0.0	1.9705641638949436	0.0
3	8.266945170863979E-4	0.0	0.0	2.402374266653072	0.0
4	8.266945170863979E-4	0.0	0.0	3.537701403451725	0.0
5	8.266945170863979E-4	0.0	0.0	8.063302754821695	0.0
6	8.266945170863979E-4	0.0	0.0	9.177411399015131	0.0
7	8.266945170863979E-4	0.0	0.0	10.81399097800717	0.0
8	8.266945170863979E-4	0.0	0.0	12.926195469162916	0.0
9	8.266945170863979E-4	0.0	0.0	13.6302636328815	0.0
10	8.266945170863979E-4	0.0	0.0	18.294749663121983	0.0
11	8.266945170863979E-4	0.0	0.0	19.6105717694845	0.0
12	8.266945170863979E-4	0.0	0.0	25.21473390081319	0.0
13	8.266945170863979E-4	0.0	0.0	26.04997092790948	0.0
14	8.266945170863979E-4	0.0	0.0	26.440446304813292	0.0
15	8.266945170863979E-4	0.0	0.0	27.38838935107236	0.0
16	8.266945170863979E-4	0.0	0.0	28.255867464335022	0.0
17	8.266945170863979E-4	0.0	0.0	29.158617876993368	0.0
18	8.266945170863979E-4	0.0	0.0	30.366143001617566	0.0
19	8.266945170863979E-4	0.0	0.0	31.672595903453104	0.0
20	8.266945170863979E-4	0.0	0.0	32.442799628538594	0.0
21	8.266945170863979E-4	0.0	0.0	33.06640285926077	0.0
22	8.266945170863979E-4	0.0	0.0	33.77047102297935	0.0
23	8.266945170863979E-4	0.0	0.0	34.34915718493983	0.0
24	8.266945170863979E-4	0.0	0.0	34.828364440010915	0.0
25	8.266945170863979E-4	0.0	0.0	35.21746199271958	0.0
26	8.266945170863979E-4	0.0	0.0	35.638525066755584	0.0
27	8.266945170863979E-4	0.0	0.0	36.14308428701732	0.0
28	8.266945170863979E-4	0.0	0.0	36.545133387160334	0.0
29	8.266945170863979E-4	0.0	0.0	36.986863824123496	0.0
30	0.0011022593561151972	0.0	0.0	37.590350821596566	0.0
31	0.0013778241951439964	0.0	0.0	38.006453728530055	0.0
32	0.0013778241951439964	0.0	0.0	38.418698727717135	0.0
33	0.0013778241951439964	0.0	0.0	38.82047226302113	0.0
34	0.0013778241951439964	0.0	0.0	39.24346429093033	0.0
35	0.0013778241951439964	0.0	0.0	39.66535405948343	0.0
36	0.0013778241951439964	0.0	0.0	40.07374115092411	0.0
37	0.0013778241951439964	0.0	0.0	40.45429619362288	0.0
38	0.0013778241951439964	0.0	0.0	40.86984797087831	0.0
39	0.0016533890341727958	0.0	0.0	41.22918452097186	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAG	25	3.8855105E-5	45.000004	1
CAACGTA	25	3.8855105E-5	45.000004	45
GCGGCTA	35	1.208864E-7	45.000004	24
CGATGTG	25	3.8855105E-5	45.000004	10
GCGAACC	40	6.7921064E-9	45.0	33
GACAATA	20	7.0268987E-4	45.0	21
TAGAACG	20	7.0268987E-4	45.0	1
TAGCGCA	20	7.0268987E-4	45.0	24
TGAACGT	20	7.0268987E-4	45.0	38
CATCTCG	20	7.0268987E-4	45.0	43
CGACAAT	20	7.0268987E-4	45.0	20
CCCGTAC	20	7.0268987E-4	45.0	31
ACGGCTA	20	7.0268987E-4	45.0	30
AAGTTAG	20	7.0268987E-4	45.0	1
AGCGCAT	20	7.0268987E-4	45.0	29
CGTAAGG	20	7.0268987E-4	45.0	2
ATGCGCG	20	7.0268987E-4	45.0	1
GTCCCAA	20	7.0268987E-4	45.0	44
ACTCCTA	20	7.0268987E-4	45.0	30
CCAACGA	20	7.0268987E-4	45.0	44
>>END_MODULE