##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935720.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	672853
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.065711232616934	33.0	31.0	34.0	30.0	34.0
2	32.114530216852714	33.0	31.0	34.0	30.0	34.0
3	32.14926885961718	34.0	31.0	34.0	30.0	34.0
4	35.79762741638961	37.0	35.0	37.0	35.0	37.0
5	34.91458461209209	37.0	35.0	37.0	33.0	37.0
6	35.35848840682883	37.0	35.0	37.0	33.0	37.0
7	35.898435468074005	37.0	35.0	37.0	35.0	37.0
8	35.869610449830795	37.0	35.0	37.0	35.0	37.0
9	37.67550118673767	39.0	38.0	39.0	35.0	39.0
10	37.37064708041727	39.0	37.0	39.0	34.0	39.0
11	37.30308551793631	39.0	37.0	39.0	34.0	39.0
12	37.15451814883786	39.0	37.0	39.0	34.0	39.0
13	37.05429714960028	39.0	37.0	39.0	33.0	39.0
14	38.05992096341994	40.0	37.0	41.0	33.0	41.0
15	38.18413234391464	40.0	37.0	41.0	33.0	41.0
16	38.23541100359217	40.0	37.0	41.0	33.0	41.0
17	38.2125843237676	40.0	37.0	41.0	34.0	41.0
18	38.21159896738218	40.0	37.0	41.0	34.0	41.0
19	38.121478242647356	40.0	37.0	41.0	34.0	41.0
20	38.08157799697705	40.0	36.0	41.0	34.0	41.0
21	38.00496542335399	40.0	36.0	41.0	33.0	41.0
22	38.07113292204984	40.0	36.0	41.0	34.0	41.0
23	38.070773259538115	40.0	36.0	41.0	34.0	41.0
24	38.04195567233853	40.0	36.0	41.0	34.0	41.0
25	37.88679399512226	40.0	36.0	41.0	34.0	41.0
26	37.901259264653646	40.0	36.0	41.0	34.0	41.0
27	37.88202772373758	40.0	36.0	41.0	34.0	41.0
28	37.7335554719976	40.0	36.0	41.0	33.0	41.0
29	37.68202861546281	40.0	36.0	41.0	33.0	41.0
30	37.55067005720417	40.0	36.0	41.0	33.0	41.0
31	37.45541299511186	40.0	35.0	41.0	33.0	41.0
32	37.38897649263658	40.0	35.0	41.0	33.0	41.0
33	37.250470756614	40.0	35.0	41.0	32.0	41.0
34	37.13341101250942	40.0	35.0	41.0	32.0	41.0
35	37.07246456506845	39.0	35.0	41.0	32.0	41.0
36	36.963757314004695	39.0	35.0	41.0	31.0	41.0
37	36.93939092193986	39.0	35.0	41.0	31.0	41.0
38	36.79017705204554	39.0	35.0	41.0	31.0	41.0
39	36.7473846441942	39.0	35.0	41.0	31.0	41.0
40	36.6191798208524	39.0	35.0	41.0	31.0	41.0
41	36.504998863050325	39.0	35.0	41.0	31.0	41.0
42	36.519698953560436	39.0	35.0	41.0	31.0	41.0
43	36.41288067378759	39.0	35.0	41.0	31.0	41.0
44	36.30295027294223	39.0	35.0	41.0	30.0	41.0
45	36.262910323651674	38.0	35.0	41.0	30.0	41.0
46	36.14122698420012	38.0	35.0	40.0	30.0	41.0
47	35.99335367457677	38.0	35.0	40.0	30.0	41.0
48	35.92790995953054	38.0	35.0	40.0	30.0	41.0
49	35.90202614835633	38.0	35.0	40.0	30.0	41.0
50	35.769184353789015	38.0	35.0	40.0	29.0	41.0
51	34.549915063171305	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	18.0
10	20.0
11	12.0
12	12.0
13	19.0
14	21.0
15	39.0
16	77.0
17	108.0
18	171.0
19	337.0
20	626.0
21	1084.0
22	1537.0
23	2193.0
24	3143.0
25	4169.0
26	5209.0
27	6106.0
28	7239.0
29	8370.0
30	10281.0
31	13238.0
32	17488.0
33	24916.0
34	45292.0
35	54171.0
36	49919.0
37	75489.0
38	128273.0
39	213102.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98398015614109	18.233403135603172	21.25739203065157	17.52522467760417
2	29.490245269026072	26.965176643338147	25.85973459284569	17.684843494790094
3	31.58639405635406	25.565911127690594	26.38213696007895	16.465557855876394
4	28.181044002181753	25.32053806700721	27.854821186797118	18.643596744013923
5	26.040903436560438	30.26991036675173	24.437730083688415	19.25145611299942
6	25.283828711471894	34.913272289786924	24.479195307147325	15.323703691593854
7	77.98850566171214	6.310888113748471	10.807709856387651	4.892896368151736
8	78.74320245283887	6.5818239645212255	9.044769065457091	5.630204517182802
9	72.18173954786559	7.443676404801643	11.76423379252229	8.610350254810486
10	40.19154258062311	25.135059217986694	18.505973815974663	16.167424385415536
11	32.070749480198494	23.687046056122213	25.495613454944838	18.74659100873445
12	27.181865875607304	21.68571738552106	29.988719675768706	21.143697063102938
13	25.975064389993058	22.78803839768865	32.74221858266218	18.494678629656107
14	21.86019828996824	26.780738140425917	31.109321055267642	20.249742514338198
15	20.003329107546524	23.31222421539326	36.09317339745829	20.591273279601936
16	24.05250478187658	23.679615012491585	30.146555042483275	22.121325163148565
17	24.433717320127872	23.694625720625456	28.091574236868976	23.780082722377696
18	25.777398629418315	22.834705351689003	29.639460625129114	21.74843539376357
19	25.09359399452778	25.49546483407223	27.172948623250548	22.237992548149446
20	25.038901513406348	26.466256373977675	27.727453098968123	20.767389013647854
21	24.427921106095983	27.163734129148565	29.098926511437117	19.309418253318334
22	23.123921569793104	23.261098635214527	29.13608172959027	24.478898065402102
23	21.484336103130996	25.766995168335434	29.29956468946412	23.449104039069454
24	22.646848568706687	24.234862592572227	28.833489632951032	24.284799205770057
25	22.786254947217298	26.970675615624813	26.89889173415293	23.344177703004966
26	22.338014395417723	24.843910928538627	27.87800604292468	24.940068633118972
27	23.552098303789982	23.641865310847987	28.241829939080304	24.564206446281727
28	21.189323670995	25.396037470294402	29.469289725987696	23.9453491327229
29	23.853501433448315	24.693060742836845	27.607516054769764	23.845921768945075
30	25.49784276803403	23.763288563772473	28.859498285658237	21.879370382535264
31	26.492710889302717	23.515537569127286	27.426198590182405	22.5655529513876
32	27.164774475256852	24.472804609624983	25.90283464590334	22.45958626921482
33	25.57988148971618	23.95471224769749	26.94392385855454	23.521482404031786
34	24.977372472144733	24.029617167494237	29.204893193609898	21.788117166751135
35	24.145690069004672	25.459944445517817	27.392610272971957	23.001755212505554
36	25.29556976040829	26.910484162216708	27.26925494870351	20.524691128671492
37	24.465819428612193	26.408888717149214	27.32498777593323	21.800304078305366
38	22.748951108191537	27.280995997639902	27.53751562376923	22.43253727039933
39	23.734158872740405	25.088243643113728	26.766470536655106	24.41112694749076
40	24.995801460348694	24.35554274113365	28.80807546373428	21.840580334783375
41	22.904705782689533	25.662514694888777	26.59005756086396	24.842721961557725
42	23.22394341706138	25.747674454895797	27.9927413565816	23.035640771461225
43	22.91362303504629	24.76320979470999	28.45822192960424	23.864945240639486
44	23.18039750138589	23.810995863881114	28.55958136472602	24.44902527000697
45	22.255232569372506	23.47169441170657	27.691040985178038	26.58203203374288
46	24.45571320927454	24.2321874168652	27.67617889791678	23.635920475943482
47	21.818584445636713	24.618601685657936	30.31360490329983	23.24920896540552
48	22.42733553985789	25.497991388906645	28.2889427556985	23.785730315536973
49	23.585538000127816	23.350122537909467	29.986044500061677	23.07829496190104
50	22.21584803813017	23.51301101429287	29.73026797829541	24.54087296928155
51	22.01298054701398	23.51078170120368	27.224371445174505	27.25186630660783
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	811.5
2	1321.0
3	2204.0
4	3087.0
5	2192.0
6	1297.0
7	1280.0
8	1263.0
9	1345.5
10	1428.0
11	1382.5
12	1337.0
13	1429.5
14	1522.0
15	1466.0
16	1410.0
17	1378.5
18	1347.0
19	1495.0
20	1643.0
21	1845.5
22	2048.0
23	2388.0
24	2728.0
25	2987.5
26	4029.5
27	4812.0
28	5797.5
29	6783.0
30	7751.5
31	8720.0
32	10346.0
33	11972.0
34	14037.5
35	16103.0
36	17066.5
37	18030.0
38	19250.0
39	20470.0
40	22855.0
41	25240.0
42	28676.0
43	32112.0
44	36751.0
45	41390.0
46	53420.5
47	65451.0
48	65686.5
49	65922.0
50	65989.0
51	66056.0
52	58212.5
53	50369.0
54	46050.5
55	41732.0
56	40013.0
57	38294.0
58	35244.0
59	32194.0
60	28978.5
61	25763.0
62	23542.0
63	21321.0
64	18969.0
65	16617.0
66	14781.0
67	12945.0
68	11007.5
69	9070.0
70	7658.0
71	6246.0
72	5496.0
73	4746.0
74	3858.5
75	2351.5
76	1732.0
77	1332.5
78	933.0
79	719.5
80	506.0
81	353.0
82	200.0
83	147.0
84	94.0
85	65.5
86	37.0
87	31.0
88	25.0
89	16.5
90	8.0
91	6.0
92	4.0
93	3.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	672853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.380247448441594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25143758810935	24.785313600936075
2	9.64567930329939	6.439503239048908
3	3.532791897534873	3.5377640317069083
4	1.8482967571329667	2.4678641244500232
5	1.2362227972747835	2.0632711437218463
6	0.9037363659499538	1.8100166114138894
7	0.7069359788618257	1.6518388533229813
8	0.6143665963581545	1.6406167208393627
9	0.5257025064730813	1.5793271775303679
>10	6.558988177866768	46.386490038196335
>50	0.13433617100482434	2.7313241745143118
>100	0.036150265278082475	2.079107678162369
>500	0.0026777974280061097	0.6043952314635089
>1k	0.0026777974280061097	2.223167374693109
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	3367	0.5004064780865954	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	3118	0.46339988080606015	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	2889	0.4293657009777767	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	2142	0.3183459091361709	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	1857	0.27598896044158233	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1550	0.23036235254951676	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT	968	0.14386500468898852	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT	776	0.11532979714737099	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4586261783777437E-4	0.0	0.0	0.15248501530051883	0.0
2	4.4586261783777437E-4	0.0	0.0	0.904358009847619	0.0
3	4.4586261783777437E-4	0.0	0.0	1.2449970498756786	0.0
4	4.4586261783777437E-4	0.0	0.0	1.7367835173507438	0.0
5	4.4586261783777437E-4	0.0	0.0	3.1760280477310796	0.0
6	4.4586261783777437E-4	0.0	0.0	4.246098330541738	0.0
7	4.4586261783777437E-4	0.0	0.0	5.173195333899083	0.0
8	4.4586261783777437E-4	0.0	0.0	6.719892755178323	0.0
9	4.4586261783777437E-4	0.0	0.0	7.4637402226043426	0.0
10	4.4586261783777437E-4	0.0	0.0	9.075830827833123	0.0
11	4.4586261783777437E-4	0.0	0.0	10.238046051663588	0.0
12	4.4586261783777437E-4	0.0	0.0	11.830072839089667	0.0
13	4.4586261783777437E-4	0.0	0.0	12.34296347047572	0.0
14	5.944834904503658E-4	0.0	0.0	12.60215827231208	0.0
15	5.944834904503658E-4	0.0	0.0	12.976237008677973	0.0
16	5.944834904503658E-4	0.0	0.0	13.56106014240852	0.0
17	5.944834904503658E-4	0.0	0.0	14.249323403477431	0.0
18	5.944834904503658E-4	0.0	0.0	15.09631375649659	0.0
19	7.431043630629573E-4	0.0	0.0	15.625255442124804	0.0
20	7.431043630629573E-4	0.0	0.0	16.121500535778246	0.0
21	7.431043630629573E-4	0.0	0.0	16.63632323850826	0.0
22	7.431043630629573E-4	0.0	0.0	17.207770493703677	0.0
23	7.431043630629573E-4	0.0	0.0	17.769706013051884	0.0
24	7.431043630629573E-4	0.0	0.0	18.211258625583895	0.0
25	7.431043630629573E-4	0.0	0.0	18.600199449211047	0.0
26	7.431043630629573E-4	0.0	0.0	18.99776028344973	0.0
27	7.431043630629573E-4	0.0	0.0	19.476022251517048	0.0
28	7.431043630629573E-4	0.0	0.0	19.90895485343753	0.0
29	7.431043630629573E-4	0.0	0.0	20.372354734243586	0.0
30	7.431043630629573E-4	0.0	0.0	21.026137952866378	0.0
31	7.431043630629573E-4	0.0	0.0	21.469176774124513	0.0
32	7.431043630629573E-4	0.0	0.0	21.90329834302589	0.0
33	8.917252356755487E-4	0.0	0.0	22.359415801073933	0.0
34	8.917252356755487E-4	0.0	0.0	22.806913248510448	0.0
35	8.917252356755487E-4	0.0	0.0	23.286661425303894	0.0
36	8.917252356755487E-4	0.0	0.0	23.74129267462581	0.0
37	8.917252356755487E-4	0.0	0.0	24.175117001781963	0.0
38	0.0010403461082881401	0.0	0.0	24.6819141773909	0.0
39	0.0010403461082881401	0.0	0.0	25.40629231050467	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGTCG	25	3.889076E-5	45.0	28
CGTCTTA	20	7.0312E-4	45.0	10
TATTGCG	35	1.2109194E-7	45.0	1
ACGATTG	25	3.889076E-5	45.0	1
TTACGCG	20	7.0312E-4	45.0	1
GCGTAAG	45	3.8380676E-10	45.0	1
GCCGATT	30	2.1641554E-6	44.999996	9
CGTAAGG	115	0.0	41.086956	2
ACAACGG	75	0.0	39.0	2
CTTACGG	35	6.2444287E-6	38.571426	2
ACGGGTA	90	0.0	37.5	5
CTCACGA	30	1.13941904E-4	37.499996	24
CAACGTT	30	1.13941904E-4	37.499996	8
TACGCAG	30	1.13941904E-4	37.499996	44
CGGTCTA	30	1.13941904E-4	37.499996	31
ACGTAAG	30	1.13941904E-4	37.499996	1
TAATGCG	60	1.546141E-10	37.499996	1
GCGATGC	225	0.0	37.0	9
TAGCCGT	55	2.744855E-9	36.81818	44
TTGATCG	25	0.0021064894	36.0	1
>>END_MODULE