##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935719.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693573
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01985371402866	33.0	31.0	34.0	30.0	34.0
2	32.05667608168138	33.0	31.0	34.0	30.0	34.0
3	32.09635034812486	33.0	31.0	34.0	30.0	34.0
4	35.747307060684314	37.0	35.0	37.0	33.0	37.0
5	34.810987451933684	37.0	35.0	37.0	33.0	37.0
6	35.28683498348408	37.0	35.0	37.0	33.0	37.0
7	35.83597833248987	37.0	35.0	37.0	35.0	37.0
8	35.800002306894875	37.0	35.0	37.0	35.0	37.0
9	37.60134982186446	39.0	37.0	39.0	35.0	39.0
10	37.311365926874316	39.0	37.0	39.0	34.0	39.0
11	37.24656669160997	39.0	37.0	39.0	34.0	39.0
12	37.17525624555743	39.0	37.0	39.0	34.0	39.0
13	37.109769267258095	39.0	37.0	39.0	33.0	39.0
14	38.22181226777859	40.0	37.0	41.0	33.0	41.0
15	38.294208396232264	40.0	37.0	41.0	34.0	41.0
16	38.32632037290956	40.0	37.0	41.0	34.0	41.0
17	38.26109292028381	40.0	37.0	41.0	34.0	41.0
18	38.23569112407778	40.0	37.0	41.0	34.0	41.0
19	38.17070156998614	40.0	37.0	41.0	34.0	41.0
20	38.130156450726886	40.0	37.0	41.0	34.0	41.0
21	38.06649912842628	40.0	37.0	41.0	34.0	41.0
22	38.100740657436205	40.0	37.0	41.0	34.0	41.0
23	38.09343068429711	40.0	37.0	41.0	34.0	41.0
24	38.05762335038994	40.0	37.0	41.0	34.0	41.0
25	37.8953275286091	40.0	36.0	41.0	33.0	41.0
26	37.91585168396117	40.0	36.0	41.0	34.0	41.0
27	37.90110630027409	40.0	36.0	41.0	34.0	41.0
28	37.72962326964862	40.0	36.0	41.0	33.0	41.0
29	37.659470596462086	40.0	36.0	41.0	33.0	41.0
30	37.557866295256595	40.0	36.0	41.0	33.0	41.0
31	37.53022969463921	40.0	36.0	41.0	33.0	41.0
32	37.48010663621565	40.0	36.0	41.0	33.0	41.0
33	37.34916584123084	40.0	35.0	41.0	33.0	41.0
34	37.259294984089635	40.0	35.0	41.0	32.0	41.0
35	37.19742406350882	40.0	35.0	41.0	32.0	41.0
36	37.13359084047389	40.0	35.0	41.0	32.0	41.0
37	37.12254513944458	40.0	35.0	41.0	32.0	41.0
38	37.017311804236904	39.0	35.0	41.0	32.0	41.0
39	36.94414142419039	39.0	35.0	41.0	31.0	41.0
40	36.852182827186176	39.0	35.0	41.0	31.0	41.0
41	36.69736422842296	39.0	35.0	41.0	31.0	41.0
42	36.71435162556789	39.0	35.0	41.0	31.0	41.0
43	36.59853396830615	39.0	35.0	41.0	31.0	41.0
44	36.49971956809161	39.0	35.0	41.0	31.0	41.0
45	36.46048072805602	39.0	35.0	41.0	31.0	41.0
46	36.319764466033135	39.0	35.0	41.0	30.0	41.0
47	36.20559479679861	38.0	35.0	41.0	30.0	41.0
48	36.15928532396734	38.0	35.0	40.0	30.0	41.0
49	36.09686795766271	38.0	35.0	40.0	30.0	41.0
50	35.966278964146525	38.0	35.0	40.0	30.0	41.0
51	34.78104828186795	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	15.0
10	12.0
11	16.0
12	11.0
13	23.0
14	11.0
15	30.0
16	47.0
17	74.0
18	188.0
19	379.0
20	672.0
21	1160.0
22	1713.0
23	2324.0
24	3266.0
25	4175.0
26	5252.0
27	6261.0
28	7086.0
29	8348.0
30	10283.0
31	13273.0
32	17035.0
33	23777.0
34	42432.0
35	56712.0
36	51095.0
37	76821.0
38	132256.0
39	228703.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.585068911275386	17.53658230640466	20.88316586718341	16.995182915136546
2	28.770727810915364	27.052235309044615	25.84111549901741	18.33592138102262
3	31.34983628255425	26.43211313012473	25.787480193144773	16.430570394176243
4	27.741420153322	24.917925005731192	27.985662648344157	19.35499219260265
5	26.117366160447425	30.4015583074889	24.040151505321	19.440924026742678
6	25.81732564560616	35.32937412500198	23.754529083456248	15.098771145935611
7	76.89889889023938	6.458728929759376	11.453156336823954	5.189215843177286
8	76.98266801043293	7.43036421544668	9.575488088492486	6.011479685627901
9	70.39979930014577	7.7061823340874	12.304833088946657	9.589185276820176
10	38.999499692173714	26.711679952939342	18.209618886548352	16.07920146833859
11	30.967612637746853	25.455864054684945	25.177306498378687	18.399216809189518
12	28.09149145079177	21.029076968105738	29.520324464764343	21.35910711633815
13	25.556358162731247	22.852965729634807	31.83918635817715	19.7514897494568
14	20.745617260187462	27.86109032502707	29.245227250772448	22.148065164013016
15	18.80638375484628	24.785134369417495	35.93969200069784	20.468789875038386
16	21.880321177439143	24.27430133525959	31.307014546414003	22.53836294088726
17	21.531547508337262	24.042602581127003	29.42300233717287	25.002847573362864
18	23.52340705304272	23.52052343444742	29.552909354891266	23.403160157618593
19	24.650901923806146	26.33623281183091	26.49987816711435	22.512987097248597
20	26.01701623333088	25.27160082644509	27.681585067469467	21.029797872754564
21	25.027214150493172	27.858639249221063	26.688034280457863	20.426112319827904
22	22.262400641316777	25.330282464859504	27.22092699686983	25.186389896953887
23	21.51655269164169	27.14234839014783	27.734643649623038	23.606455268587446
24	22.90833120666462	24.08960556423044	28.231202771734193	24.770860457370745
25	21.908869001532643	27.224819881973488	26.45979586863964	24.406515247854227
26	21.53284513670515	25.569334446410114	27.32617907559839	25.571641341286355
27	22.69609687805033	26.02898325050139	26.141444375718205	25.133475495730085
28	20.91762510939728	26.108426942801984	29.534598376811093	23.43934957098964
29	24.078215270778998	24.282519648256205	27.877526951020297	23.761738129944504
30	23.32313974159894	24.597122437003748	27.760452036051	24.319285785346313
31	23.965033240913357	24.550696177619372	27.001916164556576	24.48235441691069
32	25.92747987594673	25.155390997054383	27.388321056327165	21.528808070671722
33	23.749194389054935	23.118979545051495	27.022389856583228	26.10943620931034
34	23.247444753472237	23.573293654741462	30.27813943160994	22.901122160176364
35	22.28388359985178	23.11624010738596	29.39459869400914	25.205277598753124
36	23.150987711459354	24.908553245296456	29.211488913207408	22.728970130036778
37	22.03877601925104	24.4914378154859	31.15548038923084	22.314305776032228
38	22.40153523854014	24.897883856493838	28.63836971739096	24.062211187575063
39	22.91366590106593	22.690329640859723	30.32889111888727	24.06711333918708
40	24.987276032948227	21.261640807816914	30.111898819590728	23.63918433964413
41	20.91286713871503	23.53090446139051	30.455914518010363	25.1003138818841
42	23.482748030848953	23.59419988955741	29.206586761595393	23.71646531799825
43	23.15502477749278	22.7839030642773	29.07091250668639	24.99015965154353
44	22.24567565346402	23.235333555371966	29.22590700618392	25.293083784980098
45	21.934244845171307	22.102503990207232	28.85190167437314	27.111349490248323
46	23.898854194151156	23.76159394901474	27.739257439375525	24.600294417458578
47	21.231074450706704	23.28478761428141	31.317539754286862	24.16659818072503
48	22.119949882708813	23.031317539754287	29.26598930465863	25.58274327287827
49	22.012102547244485	21.526212813935953	32.01652890178828	24.445155737031286
50	21.23453479302107	21.726480125379734	31.26001156331057	25.778973518288627
51	20.48767757683762	22.11317337900985	28.725599179898868	28.673549864253655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	241.0
1	734.5
2	1228.0
3	1962.5
4	2697.0
5	1966.0
6	1235.0
7	1212.5
8	1190.0
9	1220.5
10	1251.0
11	1312.0
12	1373.0
13	1454.5
14	1536.0
15	1526.5
16	1517.0
17	1479.0
18	1441.0
19	1477.0
20	1513.0
21	1721.0
22	1929.0
23	2169.5
24	2410.0
25	3432.5
26	5075.0
27	5695.0
28	6272.5
29	6850.0
30	7824.5
31	8799.0
32	10369.5
33	11940.0
34	14007.5
35	16075.0
36	16931.0
37	17787.0
38	19720.0
39	21653.0
40	23943.5
41	26234.0
42	29944.5
43	33655.0
44	39010.0
45	44365.0
46	58368.5
47	72372.0
48	70239.5
49	68107.0
50	67679.5
51	67252.0
52	59245.0
53	51238.0
54	45672.5
55	40107.0
56	38101.5
57	36096.0
58	34734.0
59	33372.0
60	31916.5
61	30461.0
62	27672.0
63	24883.0
64	21588.5
65	18294.0
66	14655.0
67	11016.0
68	9209.5
69	7403.0
70	6383.5
71	5364.0
72	4688.0
73	4012.0
74	3668.0
75	2403.0
76	1482.0
77	1160.5
78	839.0
79	659.0
80	479.0
81	350.0
82	221.0
83	172.0
84	123.0
85	81.0
86	39.0
87	24.0
88	9.0
89	7.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	693573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.233260820147166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.40688080376701	23.983763957614233
2	9.81566195924407	6.327815841094217
3	3.4835896201483063	3.3686235844979326
4	1.7812947135724282	2.2966774840051767
5	1.116214817346679	1.7989621669424218
6	0.8222664476534577	1.5902597322521872
7	0.6760786939888566	1.525455461480109
8	0.5707967549462221	1.471891254198016
9	0.5075030230533605	1.4722629578182913
>10	6.560541514722448	46.015612101361384
>50	0.21172399169367973	4.209870094286021
>100	0.039838129507998685	2.1547124538445668
>500	0.004028574894067283	0.8239964392598313
>1k	0.003580955461393141	2.960096471345596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	4738	0.6831292452272508	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	4154	0.5989275822444068	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	3463	0.4992985597766926	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	2703	0.38972105315518335	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1749	0.25217244615923634	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1326	0.19118391286858052	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	1258	0.18137960964455074	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	1125	0.16220354598578665	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT	829	0.11952599077530411	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTT	717	0.10337772664160802	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC	709	0.10222427920348687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.441809297651437E-4	0.0	0.0	0.17186366828005126	0.0
2	1.441809297651437E-4	0.0	0.0	1.0026341855868093	0.0
3	1.441809297651437E-4	0.0	0.0	1.3459289793576164	0.0
4	1.441809297651437E-4	0.0	0.0	1.9323128207124556	0.0
5	1.441809297651437E-4	0.0	0.0	3.648786789566491	0.0
6	2.883618595302874E-4	0.0	0.0	4.815066330436738	0.0
7	2.883618595302874E-4	0.0	0.0	5.805877679782806	0.0
8	2.883618595302874E-4	0.0	0.0	7.434689643339634	0.0
9	2.883618595302874E-4	0.0	0.0	8.167417128406095	0.0
10	2.883618595302874E-4	0.0	0.0	9.928298823627793	0.0
11	2.883618595302874E-4	0.0	0.0	11.127163254624964	0.0
12	2.883618595302874E-4	0.0	0.0	12.888477492635959	0.0
13	4.3254278929543105E-4	0.0	0.0	13.441988082004345	0.0
14	4.3254278929543105E-4	0.0	0.0	13.700071946283952	0.0
15	5.767237190605748E-4	0.0	0.0	14.113294490990855	0.0
16	5.767237190605748E-4	0.0	0.0	14.76744336933531	0.0
17	5.767237190605748E-4	0.0	0.0	15.498008140455294	0.0
18	5.767237190605748E-4	0.0	0.0	16.413701225393723	0.0
19	5.767237190605748E-4	0.0	0.0	16.99244347747101	0.0
20	7.209046488257184E-4	0.0	0.0	17.531968516652178	0.0
21	7.209046488257184E-4	0.0	0.0	18.104366807819797	0.0
22	7.209046488257184E-4	0.0	0.0	18.72088446349555	0.0
23	7.209046488257184E-4	1.441809297651437E-4	0.0	19.309142656937336	0.0
24	7.209046488257184E-4	1.441809297651437E-4	0.0	19.771530898694152	0.0
25	7.209046488257184E-4	1.441809297651437E-4	0.0	20.180283834578336	0.0
26	7.209046488257184E-4	1.441809297651437E-4	0.0	20.5750512202753	0.0
27	7.209046488257184E-4	1.441809297651437E-4	0.0	20.991445745437034	0.0
28	7.209046488257184E-4	1.441809297651437E-4	0.0	21.39270127297343	0.0
29	7.209046488257184E-4	1.441809297651437E-4	0.0	21.830146213880873	0.0
30	8.650855785908621E-4	1.441809297651437E-4	0.0	22.38106154651349	0.0
31	0.0010092665083560057	1.441809297651437E-4	0.0	22.83177113295933	0.0
32	0.0010092665083560057	1.441809297651437E-4	0.0	23.284931795211175	0.0
33	0.0010092665083560057	1.441809297651437E-4	0.0	23.74140861884762	0.0
34	0.0010092665083560057	1.441809297651437E-4	0.0	24.17395140814305	0.0
35	0.0010092665083560057	1.441809297651437E-4	0.0	24.622786642501943	0.0
36	0.0011534474381211495	1.441809297651437E-4	0.0	25.04768784251982	0.0
37	0.0011534474381211495	1.441809297651437E-4	0.0	25.479509727166427	0.0
38	0.0011534474381211495	1.441809297651437E-4	0.0	25.920559191318002	0.0
39	0.0011534474381211495	1.441809297651437E-4	0.0	26.374729120078204	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCA	30	2.1642518E-6	45.000004	10
TTATCGC	20	7.031349E-4	45.0	31
CGTACGT	25	3.8892E-5	45.0	11
ACTCGAT	25	3.8892E-5	45.0	16
TATAGCG	35	1.2109922E-7	45.0	1
TTAAACG	20	7.031349E-4	45.0	1
CAATGCG	20	7.031349E-4	45.0	1
ACGTACG	25	3.8892E-5	45.0	10
CGCAATT	25	3.8892E-5	45.0	11
AAATGCG	45	3.8380676E-10	45.0	1
GCTACGA	65	0.0	41.538464	10
CACAACG	205	0.0	40.609756	12
ATACGAG	50	1.0804797E-9	40.5	2
TCGGATT	45	1.9264917E-8	40.0	17
TTGCACG	45	1.9264917E-8	40.0	1
ACAACGA	210	0.0	39.642857	13
GTATCGC	40	3.4555705E-7	39.375	5
AATCCGT	35	6.244707E-6	38.571426	11
TACGAAT	70	0.0	38.571426	12
CCGACGG	35	6.244707E-6	38.571426	2
>>END_MODULE