##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935718.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	612800
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69774640992167	31.0	31.0	34.0	30.0	34.0
2	31.705680483028722	31.0	31.0	34.0	30.0	34.0
3	31.7571295691906	33.0	31.0	34.0	30.0	34.0
4	35.46192558746736	37.0	35.0	37.0	33.0	37.0
5	34.52836488250653	37.0	35.0	37.0	32.0	37.0
6	34.99137891644909	37.0	35.0	37.0	32.0	37.0
7	35.4935182767624	37.0	35.0	37.0	33.0	37.0
8	35.447016971279375	37.0	35.0	37.0	33.0	37.0
9	37.18293570496083	39.0	37.0	39.0	34.0	39.0
10	36.94413348563969	39.0	37.0	39.0	33.0	39.0
11	36.95549445169713	39.0	37.0	39.0	33.0	39.0
12	36.88905515665796	39.0	35.0	39.0	33.0	39.0
13	36.71304014360313	39.0	35.0	39.0	33.0	39.0
14	37.740515665796345	40.0	36.0	41.0	33.0	41.0
15	37.77753100522193	40.0	36.0	41.0	33.0	41.0
16	37.90702676240209	40.0	36.0	41.0	33.0	41.0
17	37.78116677545692	39.0	36.0	41.0	33.0	41.0
18	37.7505319843342	39.0	36.0	41.0	33.0	41.0
19	37.74467362924282	39.0	36.0	41.0	33.0	41.0
20	37.60887565274152	39.0	36.0	41.0	32.0	41.0
21	37.59935704960836	39.0	36.0	41.0	33.0	41.0
22	37.61315437336815	39.0	36.0	41.0	33.0	41.0
23	37.638971932114885	39.0	36.0	41.0	33.0	41.0
24	37.643798955613576	39.0	36.0	41.0	33.0	41.0
25	37.43743635770235	39.0	35.0	41.0	32.0	41.0
26	37.502731723237595	39.0	36.0	41.0	33.0	41.0
27	37.44395234986945	39.0	36.0	41.0	33.0	41.0
28	37.074317885117495	39.0	35.0	41.0	31.0	41.0
29	36.8380091383812	39.0	35.0	41.0	31.0	41.0
30	36.80479112271541	39.0	35.0	41.0	31.0	41.0
31	36.877291122715405	39.0	35.0	41.0	31.0	41.0
32	36.90803687989556	39.0	35.0	41.0	31.0	41.0
33	36.74480907310705	39.0	35.0	41.0	31.0	41.0
34	36.6314703002611	39.0	35.0	41.0	30.0	41.0
35	36.60264849869451	39.0	35.0	41.0	30.0	41.0
36	36.50585998694517	39.0	35.0	41.0	30.0	41.0
37	36.41138381201044	39.0	35.0	41.0	30.0	41.0
38	36.32943701044386	39.0	35.0	41.0	30.0	41.0
39	36.31125815926893	39.0	35.0	41.0	30.0	41.0
40	36.17921181462141	39.0	35.0	40.0	30.0	41.0
41	35.96224706266319	38.0	35.0	40.0	29.0	41.0
42	35.96340404699739	38.0	35.0	40.0	29.0	41.0
43	35.83190274151436	38.0	35.0	40.0	28.0	41.0
44	35.7889703002611	38.0	35.0	40.0	28.0	41.0
45	35.89404862924282	38.0	35.0	40.0	29.0	41.0
46	35.75306951697128	38.0	35.0	40.0	28.0	41.0
47	35.65513381201044	38.0	35.0	40.0	28.0	41.0
48	35.647007180156656	38.0	35.0	40.0	28.0	41.0
49	35.48596442558747	38.0	35.0	40.0	27.0	41.0
50	35.25002774151436	38.0	35.0	40.0	26.0	41.0
51	34.0896295691906	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	12.0
11	4.0
12	9.0
13	5.0
14	15.0
15	20.0
16	36.0
17	72.0
18	155.0
19	410.0
20	938.0
21	1710.0
22	2544.0
23	3476.0
24	4288.0
25	5181.0
26	6041.0
27	6754.0
28	7767.0
29	9181.0
30	11558.0
31	14889.0
32	19497.0
33	25855.0
34	41019.0
35	54700.0
36	48010.0
37	67196.0
38	111941.0
39	169409.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.23253916449086	16.574086161879894	19.87353133159269	17.319843342036553
2	25.283126631853786	33.159758485639685	24.08583550913838	17.471279373368144
3	27.015013054830288	32.21932114882507	25.864066579634464	14.901599216710181
4	25.31837467362924	22.94990208877285	34.48253916449086	17.24918407310705
5	28.887075718015666	27.705287206266316	24.38838120104439	19.01925587467363
6	22.109823759791123	37.23449738903394	25.12679503916449	15.528883812010443
7	70.06396866840731	5.745430809399478	18.35819190600522	5.83240861618799
8	68.57424934725849	11.60231723237598	11.825228459530027	7.998204960835508
9	61.65828981723238	8.154699738903394	14.742003916449086	15.445006527415146
10	37.36112924281984	26.149314621409918	20.593178851174933	15.896377284595301
11	28.2811684073107	25.518603133159267	27.00212140992167	19.198107049608353
12	23.863250652741513	17.72878590078329	35.34791122715405	23.060052219321147
13	25.49640992167102	22.22878590078329	36.12124673629243	16.153557441253263
14	17.578655352480418	33.41073759791123	28.53590078328982	20.474706266318538
15	13.535248041775457	23.691579634464752	43.86553524804177	18.907637075718014
16	15.344973890339425	27.281821148825063	30.068537859007833	27.30466710182768
17	16.728296344647518	24.52545691906005	29.111129242819842	29.635117493472585
18	20.985639686684074	22.359823759791123	31.382996083550914	25.271540469973893
19	21.849869451697128	25.0576044386423	26.929667101827675	26.1628590078329
20	21.010933420365536	24.224869451697128	31.03720626631854	23.7269908616188
21	21.777904699738905	30.242983028720626	28.660900783289815	19.318211488250654
22	16.864719321148826	25.464425587467364	28.449249347258487	29.221605744125327
23	16.42036553524804	28.1431135770235	27.854765013054834	27.58175587467363
24	19.901599216710185	22.332082245430808	27.911553524804177	29.85476501305483
25	16.3503590078329	31.18439947780679	25.57816579634465	26.88707571801567
26	15.716383812010443	23.368635770234985	29.114556135770236	31.800424281984334
27	20.383159268929504	25.617819843342037	26.74771540469974	27.25130548302872
28	17.74330939947781	23.260443864229767	34.23580287206266	24.760443864229764
29	17.91857049608355	20.617819843342037	32.206592689295036	29.257016971279377
30	19.008159268929504	24.18798955613577	31.942558746736292	24.861292428198432
31	20.07996083550914	24.593831592689295	30.010443864229764	25.315763707571804
32	23.108518276762403	25.2960182767624	29.321475195822455	22.27398825065274
33	20.909268929503916	20.335345953002612	29.861945169712794	28.89343994778068
34	21.468505221932116	19.045039164490863	32.92460835509139	26.56184725848564
35	21.056625326370757	20.11047650130548	31.6628590078329	27.170039164490863
36	23.805319843342037	18.819353785900784	32.5246409921671	24.85068537859008
37	19.48172323759791	20.952839425587467	39.27203002610966	20.29340731070496
38	20.66710182767624	21.393929503916446	29.853296344647518	28.08567232375979
39	24.65861618798956	18.944843342036556	32.89409268929504	23.50244778067885
40	25.597095300261095	17.50179503916449	30.404862924281982	26.496246736292427
41	21.963446475195823	20.16334856396867	28.9095953002611	28.963609660574413
42	25.827513054830288	19.363413838120106	31.377774151436032	23.431298955613578
43	26.104601827676237	19.75669060052219	28.932930809399476	25.20577676240209
44	20.548466057441253	18.72780678851175	32.20251305483029	28.521214099216714
45	18.786390339425587	18.574902088772845	29.941253263707573	32.697454308093995
46	25.222584856396868	22.3782637075718	28.574412532637076	23.824738903394255
47	18.27186684073107	18.315763707571804	37.13446475195823	26.2779046997389
48	21.178198433420363	19.995920365535248	29.8519908616188	28.97389033942559
49	22.411063968668408	15.761749347258485	37.37956919060052	24.447617493472585
50	22.43325718015666	16.39947780678851	34.40926892950392	26.75799608355091
51	21.404536553524807	17.22943864229765	28.27888381201044	33.0871409921671
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	306.0
1	484.5
2	663.0
3	1430.5
4	2198.0
5	1515.0
6	832.0
7	862.5
8	893.0
9	936.5
10	980.0
11	1047.0
12	1114.0
13	1120.5
14	1127.0
15	1126.5
16	1126.0
17	1192.0
18	1258.0
19	1248.0
20	1238.0
21	1239.0
22	1240.0
23	1322.5
24	1405.0
25	1853.5
26	2940.0
27	3578.0
28	3725.5
29	3873.0
30	5585.5
31	7298.0
32	7530.5
33	7763.0
34	8946.0
35	10129.0
36	11213.0
37	12297.0
38	13473.5
39	14650.0
40	17308.5
41	19967.0
42	24818.0
43	29669.0
44	38193.0
45	46717.0
46	80889.5
47	115062.0
48	96616.5
49	78171.0
50	75423.5
51	72676.0
52	57718.0
53	42760.0
54	36321.0
55	29882.0
56	27190.5
57	24499.0
58	23296.5
59	22094.0
60	19827.0
61	17560.0
62	15298.5
63	13037.0
64	10545.5
65	8054.0
66	6826.0
67	5598.0
68	4512.5
69	3427.0
70	3012.5
71	2598.0
72	2189.5
73	1781.0
74	1675.5
75	1128.0
76	686.0
77	484.0
78	282.0
79	250.5
80	219.0
81	155.0
82	91.0
83	81.0
84	71.0
85	57.5
86	44.0
87	26.0
88	8.0
89	6.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	612800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.15673133214809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54283831442983	25.461397010417343
2	11.2259941463812	7.668865319884195
3	4.39652803380034	4.505130805343265
4	2.2418405484179202	3.062957812073057
5	1.2513462332540624	2.137094854637726
6	0.7868823989636324	1.6126398414838174
7	0.5774529477761486	1.3806733635902795
8	0.4405999732425603	1.2039563928798216
9	0.35179173036775246	1.0814450057138523
>10	3.7891210254743597	29.78165011750187
>50	0.3370321150734078	7.191339405261496
>100	0.04897047825833661	3.1451143240611765
>500	0.0028806163681374475	0.6108530610108912
>1k	0.004801027280229079	4.021626971337207
>5k	4.8010272802290796E-4	1.017213835557197
>10k+	0.0014403081840687238	6.1180418792468005
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	14084	2.298302872062663	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGC	12437	2.0295365535248044	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG	10787	1.7602806788511751	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	6203	1.012238903394256	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTT	3998	0.6524151436031331	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	3749	0.6117819843342037	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	3209	0.5236618798955613	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCT	2720	0.44386422976501305	No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	2287	0.37320496083550914	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTC	2226	0.36325065274151436	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTA	1965	0.3206592689295039	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTCTGGT	1836	0.2996083550913838	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1285	0.20969321148825065	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	1249	0.20381853785900783	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	801	0.13071148825065274	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	708	0.11553524804177545	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATG	674	0.10998694516971279	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.527415143603133E-4	0.0	0.0	0.39033942558746737	0.0
2	6.527415143603133E-4	0.0	0.0	2.452676240208877	0.0
3	6.527415143603133E-4	0.0	0.0	3.1533942558746735	0.0
4	6.527415143603133E-4	0.0	0.0	4.462140992167102	0.0
5	6.527415143603133E-4	0.0	0.0	8.693864229765014	0.0
6	6.527415143603133E-4	0.0	0.0	10.760280678851174	0.0
7	6.527415143603133E-4	0.0	0.0	12.549934725848564	0.0
8	6.527415143603133E-4	0.0	0.0	15.158126631853786	0.0
9	6.527415143603133E-4	0.0	0.0	16.087304177545693	0.0
10	6.527415143603133E-4	0.0	0.0	19.72911227154047	0.0
11	6.527415143603133E-4	0.0	0.0	21.72584856396867	0.0
12	6.527415143603133E-4	0.0	0.0	25.89947780678851	0.0
13	6.527415143603133E-4	0.0	0.0	27.010280678851174	0.0
14	6.527415143603133E-4	0.0	0.0	27.52023498694517	0.0
15	6.527415143603133E-4	0.0	0.0	28.37385770234987	0.0
16	6.527415143603133E-4	0.0	0.0	29.49804177545692	0.0
17	6.527415143603133E-4	0.0	0.0	30.818048302872064	0.0
18	6.527415143603133E-4	0.0	0.0	32.69810704960835	0.0
19	6.527415143603133E-4	0.0	0.0	33.878263707571804	0.0
20	6.527415143603133E-4	0.0	0.0	34.86798302872063	0.0
21	6.527415143603133E-4	0.0	0.0	35.7894908616188	0.0
22	6.527415143603133E-4	0.0	0.0	36.670691906005224	0.0
23	6.527415143603133E-4	0.0	0.0	37.44125326370757	0.0
24	6.527415143603133E-4	0.0	0.0	38.0509138381201	0.0
25	6.527415143603133E-4	0.0	0.0	38.585998694516974	0.0
26	6.527415143603133E-4	0.0	0.0	39.162206266318535	0.0
27	6.527415143603133E-4	0.0	0.0	39.775456919060055	0.0
28	6.527415143603133E-4	0.0	0.0	40.27447780678851	0.0
29	8.159268929503916E-4	0.0	0.0	40.81168407310705	0.0
30	9.791122715404699E-4	0.0	0.0	41.53296344647519	0.0
31	9.791122715404699E-4	0.0	0.0	42.115372062663184	0.0
32	9.791122715404699E-4	0.0	0.0	42.630711488250654	0.0
33	9.791122715404699E-4	0.0	0.0	43.23368146214099	0.0
34	9.791122715404699E-4	0.0	0.0	43.89817232375979	0.0
35	9.791122715404699E-4	0.0	0.0	44.496899477806785	0.0
36	9.791122715404699E-4	0.0	0.0	45.00179503916449	0.0
37	9.791122715404699E-4	0.0	0.0	45.491840731070496	0.0
38	9.791122715404699E-4	0.0	0.0	45.972095300261095	0.0
39	9.791122715404699E-4	0.0	0.0	46.599706266318535	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	25	3.888666E-5	45.000004	1
ACCGAAC	25	3.888666E-5	45.000004	24
ATTCGGG	25	3.888666E-5	45.000004	3
CACGAAG	25	3.888666E-5	45.000004	1
TTATCGG	25	3.888666E-5	45.000004	2
TCAGGCG	35	1.210683E-7	45.000004	1
CTAACGG	25	3.888666E-5	45.000004	2
TGCGACG	25	3.888666E-5	45.000004	1
CGGTCGA	25	3.888666E-5	45.000004	41
CTCTATA	25	3.888666E-5	45.000004	32
ATGTTAG	35	1.210683E-7	45.000004	1
CGAAGTA	25	3.888666E-5	45.000004	23
AAGTCCG	50	2.1827873E-11	45.000004	11
TCGTTTA	20	7.0307066E-4	45.0	38
TTGGCCG	20	7.0307066E-4	45.0	1
GTCGACG	20	7.0307066E-4	45.0	1
CTGAGCG	45	3.8380676E-10	45.0	1
CGCATCG	20	7.0307066E-4	45.0	21
CCGCTAT	20	7.0307066E-4	45.0	29
GCCGCAT	20	7.0307066E-4	45.0	19
>>END_MODULE