##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935717.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234145
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42760255397297	34.0	31.0	34.0	31.0	34.0
2	32.637690320100795	34.0	31.0	34.0	31.0	34.0
3	32.4715923893314	34.0	31.0	34.0	31.0	34.0
4	36.09515471182387	37.0	35.0	37.0	35.0	37.0
5	36.12534967648252	37.0	35.0	37.0	35.0	37.0
6	36.147058446689016	37.0	37.0	37.0	35.0	37.0
7	36.27980524888424	37.0	37.0	37.0	35.0	37.0
8	36.03682333596703	37.0	37.0	37.0	35.0	37.0
9	37.85039185120331	39.0	38.0	39.0	35.0	39.0
10	37.73100856306989	39.0	38.0	39.0	35.0	39.0
11	37.74019090734374	39.0	38.0	39.0	35.0	39.0
12	37.59585726793227	39.0	37.0	39.0	35.0	39.0
13	37.54277477631382	39.0	37.0	39.0	35.0	39.0
14	38.9620534284311	41.0	38.0	41.0	35.0	41.0
15	39.0804330649811	41.0	39.0	41.0	35.0	41.0
16	39.13499754425676	41.0	39.0	41.0	35.0	41.0
17	39.08832133934101	41.0	39.0	41.0	35.0	41.0
18	38.41453799995729	39.0	38.0	40.0	35.0	41.0
19	37.69567575647569	37.0	37.0	40.0	35.0	41.0
20	36.87018300625681	35.0	35.0	40.0	34.0	41.0
21	36.823903991116616	35.0	35.0	40.0	34.0	41.0
22	36.81469602169596	35.0	35.0	40.0	34.0	41.0
23	36.831442055136776	35.0	35.0	40.0	34.0	41.0
24	36.66096222426275	35.0	35.0	40.0	34.0	41.0
25	36.5611010271413	35.0	35.0	40.0	34.0	41.0
26	36.404087210916316	35.0	35.0	40.0	33.0	41.0
27	36.31828994853616	35.0	35.0	40.0	33.0	41.0
28	36.16574344957185	35.0	35.0	40.0	33.0	41.0
29	35.90677144504474	35.0	35.0	40.0	33.0	41.0
30	35.409891306668946	35.0	35.0	40.0	31.0	41.0
31	34.74640927630315	35.0	35.0	40.0	24.0	41.0
32	33.71132845031924	35.0	34.0	40.0	18.0	41.0
33	32.534544833329775	35.0	33.0	40.0	12.0	41.0
34	31.546554485468405	35.0	30.0	40.0	8.0	41.0
35	30.9887847274125	35.0	24.0	40.0	7.0	41.0
36	30.626240150334194	35.0	23.0	40.0	7.0	41.0
37	30.43713937944436	35.0	23.0	40.0	7.0	41.0
38	30.276439812936427	35.0	22.0	40.0	7.0	41.0
39	30.171517649319867	35.0	22.0	40.0	7.0	41.0
40	30.050161224882018	35.0	21.0	40.0	7.0	41.0
41	29.86689444574943	35.0	21.0	40.0	7.0	41.0
42	29.733566806893165	35.0	20.0	40.0	7.0	41.0
43	29.570253475410535	35.0	18.0	40.0	7.0	41.0
44	29.428055264899957	35.0	18.0	40.0	7.0	41.0
45	29.394170279100557	35.0	20.0	40.0	7.0	41.0
46	29.331448461423477	35.0	20.0	40.0	7.0	41.0
47	29.360631232783103	35.0	20.0	40.0	7.0	41.0
48	29.337483183497405	35.0	20.0	40.0	7.0	41.0
49	29.316718272865106	35.0	20.0	40.0	7.0	41.0
50	29.184466890174892	35.0	20.0	39.0	7.0	41.0
51	28.59391402763245	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	3.0
11	4.0
12	1.0
13	3.0
14	5.0
15	7.0
16	32.0
17	56.0
18	111.0
19	236.0
20	388.0
21	602.0
22	991.0
23	1636.0
24	3335.0
25	6869.0
26	12196.0
27	15191.0
28	13626.0
29	10518.0
30	8123.0
31	6838.0
32	6351.0
33	6924.0
34	11357.0
35	18426.0
36	17468.0
37	14935.0
38	26642.0
39	51207.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.87157530590019	12.679749727732814	14.126289265198915	47.32238570116808
2	59.741186017211554	15.12823250549873	14.166862414315915	10.963719062973798
3	19.633560400606463	15.562151658160541	54.248862884110274	10.555425057122724
4	19.058275854705418	15.956778919045892	53.398535095773994	11.586410130474706
5	16.162634265092144	18.168229088812488	52.77797945717397	12.891157188921396
6	15.630912468769353	21.587477844925154	53.13801276986483	9.643596916440666
7	48.63268487475709	4.022293877725342	43.85658459501591	3.488436652501655
8	48.0035875205535	3.924491234064362	44.221315851288736	3.850605394093404
9	44.2828162036345	5.3249055072711355	45.693907621345744	4.698370667748617
10	25.336436823335966	15.512609707659783	49.66665954857033	9.484293920433919
11	20.839223558051636	15.211941318413805	52.01306882487348	11.935766298661086
12	19.51184095325546	13.588161182173438	55.03341946229901	11.866578402272095
13	15.523713937944436	15.943539259860342	55.74793397253839	12.784812829656836
14	12.183476051164877	18.590616925409467	55.187597428943604	14.038309594482051
15	11.910995323410708	16.01400841358987	59.675414807064	12.399581455935426
16	13.172179632279143	15.237566465224539	58.18104166221786	13.409212240278459
17	12.611416002904182	15.117982446774434	55.6544021866792	16.61619936364219
18	13.90078797326443	16.535053065408185	55.61724572380362	13.946913237523756
19	15.881611821734396	16.87031540284866	53.820068760810614	13.428004014606335
20	17.410151828994856	15.284972986824405	54.64306305921545	12.6618121249653
21	14.672104892267612	16.85921117256401	55.015908945311665	13.452774989856714
22	13.55527557709966	16.067821221892416	54.20743556343292	16.169467637575007
23	14.663136090883855	16.075935851715816	54.87838732409405	14.382540733306284
24	14.898887441542632	15.000106771445045	55.55531828567768	14.545687501334642
25	13.582181981250935	16.702043605458154	54.46411411732046	15.251660295970446
26	12.698541502060689	18.32155288389673	54.57729184906789	14.402613764974696
27	14.954408592965898	17.251275918768286	54.45770783061777	13.33660765764804
28	12.370966708663435	17.51008990155673	55.944820517200874	14.174122872578957
29	13.606525870721134	16.80710670738218	55.40370283371415	14.182664588182536
30	15.135920049541951	16.443229622669715	53.56851523628521	14.852335091503127
31	15.641162527493647	18.119541309872087	51.24346024899101	14.995835913643255
32	16.15665506416964	18.787076384291783	49.72559738623502	15.33067116530355
33	16.267697367016165	20.161438424907644	47.32452113006897	16.246343078007218
34	15.537807768690342	21.571675671058532	46.05265967669606	16.837856883555062
35	16.54658438147302	22.105532896282217	42.54585833564671	18.80202438659805
36	17.402464284951634	24.662495462213585	41.651113626171814	16.283926626662964
37	18.20495846590788	24.082512972730573	42.376732366695855	15.335796194665699
38	16.236093019282922	24.0799504580495	41.27527813961434	18.408678383053235
39	17.860300241303463	23.15360140084136	41.262465566208974	17.723632791646203
40	17.78171645775054	23.052809156719125	41.6472698541502	17.51820453138013
41	16.03792521727989	24.711183241153986	39.73520681628905	19.515684725277072
42	16.914305238207096	24.057314911700015	40.10933395972581	18.91904589036708
43	17.694590958594034	23.63663541822375	40.037156462875565	18.63161716030665
44	16.89209677763779	23.946272608853487	39.79884259753572	19.36278801597301
45	17.226077857737724	23.199726665100687	39.35723590083068	20.216959576330908
46	17.113754297550663	23.612291528753552	39.97437485318927	19.299579320506524
47	16.381729270323945	22.65220269491127	42.560379252172794	18.405688782591984
48	15.933289201136047	21.099318798180615	43.13822631275492	19.82916568792842
49	15.90766405432531	20.91396356958295	43.9317516923274	19.246620683764334
50	14.892908240620129	20.96393260586389	43.78696961284674	20.35618954066924
51	15.056909180208844	20.793525379572486	42.073074376988615	22.07649106323005
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	97.0
1	181.0
2	265.0
3	15797.0
4	31329.0
5	20419.0
6	9509.0
7	9050.0
8	8591.0
9	8292.0
10	7993.0
11	7535.0
12	7077.0
13	6544.5
14	6012.0
15	5423.5
16	4835.0
17	4472.5
18	4110.0
19	3774.0
20	3438.0
21	3097.5
22	2757.0
23	2610.0
24	2463.0
25	2336.0
26	2260.0
27	2311.0
28	2324.0
29	2337.0
30	2387.5
31	2438.0
32	2657.5
33	2877.0
34	3311.0
35	3745.0
36	3729.5
37	3714.0
38	4155.0
39	4596.0
40	5122.5
41	5649.0
42	6813.5
43	7978.0
44	9063.0
45	10148.0
46	11467.0
47	12786.0
48	13731.0
49	14676.0
50	14900.5
51	15125.0
52	12975.0
53	10825.0
54	9527.5
55	8230.0
56	7606.0
57	6982.0
58	6823.0
59	6664.0
60	6279.0
61	5894.0
62	5505.5
63	5117.0
64	4547.0
65	3977.0
66	3242.5
67	2508.0
68	2191.5
69	1875.0
70	1486.0
71	1097.0
72	969.0
73	841.0
74	693.5
75	393.5
76	241.0
77	207.5
78	174.0
79	130.0
80	86.0
81	47.0
82	8.0
83	9.5
84	11.0
85	6.5
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	234145.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.76538170591896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.034911848603	35.02758466853687
2	7.878078214524185	6.895742003354711
3	2.740801354446596	3.598566523723649
4	1.4765762155391635	2.584916867638111
5	0.9937017732004805	2.1744868702983773
6	0.8417467029400735	2.210361945232266
7	0.7077869792200956	2.168359711843278
8	0.636808339568161	2.229612804377042
9	0.6018188702510929	2.37049493169276
>10	3.9748037011463935	27.322819857425234
>50	0.08797352256057488	2.5183774135587282
>100	0.02199338064014372	1.859469077071681
>500	0.0019993982400130657	0.5285267400241389
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	9.996991200065329E-4	8.510680585223138
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19452	8.307672596040915	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	634	0.27077238463345366	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	574	0.24514723782271666	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	383	0.16357385380853745	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	347	0.14819876572209528	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	311	0.13282367763565311	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	299	0.12769864827350574	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	265	0.11317773174742148	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	236	0.10079224412223196	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA	235	0.10036515834205299	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17168848363193748	0.0
2	0.0	0.0	0.0	0.853744474577719	0.0
3	0.0	0.0	0.0	1.2167673877298255	0.0
4	0.0	0.0	0.0	1.614384249076427	0.0
5	0.0	0.0	0.0	2.668004868777894	0.0
6	0.0	0.0	0.0	3.218945525208738	0.0
7	0.0	0.0	0.0	3.763052809156719	0.0
8	0.0	0.0	0.0	4.626193170898375	0.0
9	0.0	0.0	0.0	4.939247047769545	0.0
10	0.0	0.0	0.0	5.64393858506481	0.0
11	4.2708578017894896E-4	0.0	0.0	6.674923658416793	0.0
12	4.2708578017894896E-4	0.0	0.0	7.520126417390933	0.0
13	4.2708578017894896E-4	0.0	0.0	7.876315958060176	0.0
14	4.2708578017894896E-4	0.0	0.0	8.02109803754084	0.0
15	4.2708578017894896E-4	0.0	0.0	8.295714194195904	0.0
16	4.2708578017894896E-4	0.0	0.0	8.82145678959619	0.0
17	4.2708578017894896E-4	0.0	0.0	9.407418480001708	0.0
18	4.2708578017894896E-4	0.0	0.0	10.143287279250037	0.0
19	4.2708578017894896E-4	0.0	0.0	10.510581050203934	0.0
20	4.2708578017894896E-4	0.0	0.0	10.889406137222661	0.0
21	4.2708578017894896E-4	0.0	0.0	11.336137863289842	0.0
22	4.2708578017894896E-4	0.0	0.0	11.823869824254201	0.0
23	4.2708578017894896E-4	0.0	0.0	12.28341412372675	0.0
24	4.2708578017894896E-4	0.0	0.0	12.6536974951419	0.0
25	4.2708578017894896E-4	0.0	0.0	12.964615943112173	0.0
26	4.2708578017894896E-4	0.0	0.0	13.269555190159943	0.0
27	4.2708578017894896E-4	0.0	0.0	13.548015118836618	0.0
28	4.2708578017894896E-4	0.0	0.0	13.870037797091546	0.0
29	4.2708578017894896E-4	0.0	0.0	14.195050075807726	0.0
30	4.2708578017894896E-4	0.0	0.0	14.540989557752674	0.0
31	4.2708578017894896E-4	0.0	0.0	14.86429349334814	0.0
32	4.2708578017894896E-4	0.0	0.0	15.214076747314698	0.0
33	8.541715603578979E-4	0.0	0.0	15.537380682910163	0.0
34	8.541715603578979E-4	0.0	0.0	15.838049072156142	0.0
35	8.541715603578979E-4	0.0	0.0	16.14896752012642	0.0
36	8.541715603578979E-4	0.0	0.0	16.453479681394008	0.0
37	8.541715603578979E-4	0.0	0.0	16.779773217450725	0.0
38	8.541715603578979E-4	0.0	0.0	17.096670866343505	0.0
39	8.541715603578979E-4	0.0	0.0	17.397339255589486	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAT	30	2.1580727E-6	45.000004	20
TAGCATA	30	2.1580727E-6	45.000004	30
GTAGCAT	30	2.1580727E-6	45.000004	29
TCGACAA	30	2.1580727E-6	45.000004	19
CTCAGAT	60	0.0	45.000004	16
AACAAGC	25	3.881261E-5	45.0	13
CGGAAGG	20	7.0217694E-4	45.0	2
GGTACGG	20	7.0217694E-4	45.0	2
AGTTTAG	25	3.881261E-5	45.0	1
ACGTTAG	25	3.881261E-5	45.0	1
GAATATC	20	7.0217694E-4	45.0	22
TACGGGA	25	3.881261E-5	45.0	4
TGTGCGG	25	3.881261E-5	45.0	2
CCGCTAT	20	7.0217694E-4	45.0	25
TTGAGCG	20	7.0217694E-4	45.0	1
CGCTATC	20	7.0217694E-4	45.0	26
CTACGGG	20	7.0217694E-4	45.0	3
ATTGGGA	50	2.1827873E-11	45.0	4
ATAGTAG	20	7.0217694E-4	45.0	1
AATAGGG	35	1.2063902E-7	45.0	3
>>END_MODULE