##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935715.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665223
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.437472847451154	34.0	31.0	34.0	31.0	34.0
2	32.64675605022676	34.0	31.0	34.0	31.0	34.0
3	32.47707911482315	34.0	31.0	34.0	31.0	34.0
4	36.08445739248342	37.0	35.0	37.0	35.0	37.0
5	36.11615202721494	37.0	35.0	37.0	35.0	37.0
6	36.114683346787466	37.0	36.0	37.0	35.0	37.0
7	36.262158704674974	37.0	37.0	37.0	35.0	37.0
8	36.048057568664944	37.0	37.0	37.0	35.0	37.0
9	37.86117136659436	39.0	38.0	39.0	35.0	39.0
10	37.7161327855471	39.0	38.0	39.0	35.0	39.0
11	37.66251317227456	39.0	38.0	39.0	35.0	39.0
12	37.5028945180789	39.0	37.0	39.0	35.0	39.0
13	37.46521392074538	39.0	37.0	39.0	35.0	39.0
14	38.72781909224425	41.0	38.0	41.0	35.0	41.0
15	38.86006196418344	41.0	38.0	41.0	35.0	41.0
16	38.94044403155032	41.0	38.0	41.0	35.0	41.0
17	38.91196035013822	41.0	38.0	41.0	35.0	41.0
18	38.2299168850145	39.0	38.0	40.0	35.0	41.0
19	37.517152894593245	37.0	37.0	40.0	35.0	41.0
20	36.67120198790481	35.0	35.0	40.0	34.0	41.0
21	36.63136722572731	35.0	35.0	40.0	33.0	41.0
22	36.636914237781916	35.0	35.0	40.0	34.0	41.0
23	36.65387697057979	35.0	35.0	40.0	34.0	41.0
24	36.490195017309986	35.0	35.0	40.0	33.0	41.0
25	36.41026242327761	35.0	35.0	40.0	33.0	41.0
26	36.26056976382356	35.0	35.0	40.0	33.0	41.0
27	36.1766595562691	35.0	35.0	40.0	33.0	41.0
28	36.00154384319244	35.0	35.0	40.0	33.0	41.0
29	35.733439763808526	35.0	35.0	40.0	33.0	41.0
30	35.16661029459294	35.0	35.0	40.0	30.0	41.0
31	34.42444714028228	35.0	35.0	40.0	23.0	41.0
32	33.32197172978084	35.0	33.0	40.0	16.0	41.0
33	32.08771043695122	35.0	31.0	40.0	10.0	41.0
34	31.02465639341995	35.0	25.0	40.0	7.0	41.0
35	30.406036772631133	35.0	23.0	40.0	7.0	41.0
36	30.039541627394122	35.0	21.0	40.0	7.0	41.0
37	29.84197780293225	35.0	21.0	40.0	7.0	41.0
38	29.682649277009364	35.0	20.0	40.0	7.0	41.0
39	29.529240570455322	35.0	18.0	40.0	7.0	41.0
40	29.383626242628413	35.0	18.0	40.0	7.0	41.0
41	29.194044703806092	35.0	18.0	40.0	7.0	41.0
42	29.107350467437236	35.0	18.0	40.0	7.0	41.0
43	29.00910070758227	35.0	18.0	40.0	7.0	41.0
44	28.892117380186793	35.0	18.0	40.0	7.0	41.0
45	28.857339568836316	35.0	18.0	40.0	7.0	41.0
46	28.860167192054394	35.0	18.0	40.0	7.0	41.0
47	28.894199388776396	35.0	19.0	39.0	7.0	41.0
48	28.855982129301	35.0	20.0	39.0	7.0	41.0
49	28.861508095781414	35.0	20.0	39.0	7.0	41.0
50	28.731592263045624	35.0	19.0	39.0	7.0	41.0
51	28.09223974516816	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	8.0
10	10.0
11	19.0
12	3.0
13	22.0
14	30.0
15	50.0
16	103.0
17	199.0
18	455.0
19	767.0
20	1311.0
21	2070.0
22	3214.0
23	5653.0
24	10788.0
25	22246.0
26	37575.0
27	43914.0
28	39752.0
29	30521.0
30	23718.0
31	20265.0
32	19164.0
33	22161.0
34	38670.0
35	46095.0
36	43944.0
37	43632.0
38	76041.0
39	132692.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.71303156986454	12.940773244460878	14.105796101457708	47.240399084216875
2	60.37584388994367	14.448237658649807	14.361048851287464	10.814869600119058
3	20.334233783257645	15.045330663551923	53.99392384208003	10.626511711110409
4	17.916397959781907	15.695789231580989	54.60529777232597	11.782515036311132
5	16.211556124788228	17.91053526411444	53.63359354682565	12.24431506427168
6	16.48710282115922	20.749282571408383	53.273413577101216	9.490201030331182
7	48.9613257509136	3.75753694625712	43.99577284609823	3.285364456731051
8	49.53166081148728	3.4299776165285927	43.66114821646275	3.3772133555213815
9	46.084997061135894	4.3170485686754665	44.96432023546991	4.633634134718734
10	27.97888828257592	13.965241730968412	48.87819573285951	9.177674253596162
11	21.0064895531273	14.850809427815935	52.35236905518901	11.790331963867756
12	19.003101215682562	13.453984603659224	55.073411472543796	12.469502708114423
13	17.47624480813201	13.243679187280055	56.62251605852473	12.657559946063198
14	13.760799010256711	15.106813805295365	57.40631337160621	13.726073812841708
15	13.58010772327475	14.58939333125884	58.81290935520871	13.017589590257703
16	16.96994842331068	14.634040013649557	55.84909120700878	12.546920356030986
17	16.42847586448454	15.370484784801489	54.95495495495496	13.246084395759016
18	16.896890215762234	14.673725953552418	55.86231985364306	12.567063977042285
19	16.7068787459243	16.24823555409239	54.289163182872514	12.755722517110804
20	16.561664284007016	16.890426217975023	54.62889888052578	11.919010617492178
21	16.39375066706954	15.944577983623537	56.03970397896645	11.621967370340473
22	15.097192971379522	14.620059739365596	56.68745668745668	13.595290601798194
23	14.764672899163136	14.971220177293931	56.61965987345597	13.644447050086963
24	15.632652509008258	15.255485754401155	55.5512963322074	13.560565404383192
25	15.6276917665204	15.449856664607205	54.38822770709972	14.534223861772668
26	14.243644612408172	15.257289660760376	54.934210031823916	15.564855695007541
27	14.551811948775073	14.936194328819058	55.92064615925787	14.591347563147997
28	13.214666360002585	16.628859795887998	54.650545756836436	15.505928087272991
29	15.555084535561758	16.731081156243846	53.823454691133655	13.890379617060745
30	17.074274341085623	15.670534542551895	53.70785435861358	13.547336757748903
31	16.765054726009172	17.219939779592707	52.015339217074576	13.999666277323545
32	18.1773630797492	18.362714458159143	49.00612275883425	14.453799703257403
33	18.35820469226109	20.3764752571694	45.943841388526856	15.321478662042654
34	16.94469373428159	22.37400089894667	45.298042911925776	15.383262454845969
35	17.852960586149305	24.350180315473157	41.737282084353666	16.05957701402387
36	19.638226579658248	26.073512190648852	38.96918777612921	15.319073453563693
37	19.09344685917354	26.1213157091682	38.93145606811551	15.853781363542751
38	19.19070747704153	25.94934330292248	37.92472599414031	16.935223225895676
39	19.239713599800368	24.676537040962206	38.28761182340358	17.796137535833846
40	19.975256417772684	23.45439048259005	39.138454322836104	17.431898776801162
41	17.971717754798014	24.6186617119372	38.18734469493689	19.2222758383279
42	18.601281074166106	24.585439769821548	38.536851552035934	18.276427603976412
43	18.899226274497423	24.081999570068984	38.65034732713691	18.368426828296677
44	18.62503250789585	23.626513214365712	38.72370618574523	19.02474809199321
45	18.706358619590723	22.66052135900292	38.88034538793758	19.752774633468775
46	18.53619011970422	22.92795047675742	39.876853325877185	18.659006077661175
47	16.814962801947615	23.2373204173638	41.91301262884777	18.03470415184081
48	17.002118086716784	23.164412535345292	41.61416547533684	18.219303902601084
49	18.239898500202187	21.83628647836891	42.01778952321252	17.906025498216387
50	16.924850764330156	21.935801979185925	42.08303080320434	19.056316453279575
51	16.85870753115872	21.528570118591812	41.425506935268324	20.18721541498114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	392.0
2	658.0
3	42159.5
4	83661.0
5	54803.5
6	25946.0
7	24672.5
8	23399.0
9	22762.0
10	22125.0
11	21449.5
12	20774.0
13	19675.5
14	18577.0
15	16989.5
16	15402.0
17	14099.5
18	12797.0
19	11547.5
20	10298.0
21	9225.0
22	8152.0
23	7586.5
24	7021.0
25	6652.5
26	6180.5
27	6077.0
28	6263.5
29	6450.0
30	6849.0
31	7248.0
32	7779.0
33	8310.0
34	9171.0
35	10032.0
36	10890.5
37	11749.0
38	12551.5
39	13354.0
40	14875.0
41	16396.0
42	17680.0
43	18964.0
44	21138.5
45	23313.0
46	27639.0
47	31965.0
48	34349.0
49	36733.0
50	37387.0
51	38041.0
52	34542.0
53	31043.0
54	29433.0
55	27823.0
56	26427.0
57	25031.0
58	24033.0
59	23035.0
60	21235.5
61	19436.0
62	17903.0
63	16370.0
64	14373.0
65	12376.0
66	10503.0
67	8630.0
68	7463.5
69	6297.0
70	5429.0
71	4561.0
72	3801.0
73	3041.0
74	2382.0
75	1340.5
76	958.0
77	707.0
78	456.0
79	368.5
80	281.0
81	244.0
82	207.0
83	138.0
84	69.0
85	47.0
86	25.0
87	14.5
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	665223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.59412489187585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.47408466385615	35.886713820593926
2	7.22304618463918	6.442108473151739
3	2.752844799010053	3.6828211432501554
4	1.6041198465278006	2.861372831103899
5	1.1251229626356463	2.508693695724569
6	0.9150756578139627	2.448419890204278
7	0.7787527661250101	2.430945867873074
8	0.6696660382243321	2.3890536755538863
9	0.6087747297177808	2.443299869524741
>10	3.7390457084914805	25.40593405323098
>50	0.06100308375552756	1.9104617976021212
>100	0.04473559311048302	3.5951320280282437
>500	0.0033890600841274834	1.0742474548185923
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.3890600841274835E-4	6.920795399339871
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45793	6.883856992316863	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	979	0.14716869380643785	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC	869	0.13063288551357966	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	847	0.127325723855008	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC	746	0.1121428453315655	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	733	0.11018861344240954	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.006510598701488E-4	0.0	0.0	0.07501243943760212	0.0
2	3.006510598701488E-4	0.0	0.0	0.39385288842989497	0.0
3	3.006510598701488E-4	0.0	0.0	0.5858185901569849	0.0
4	3.006510598701488E-4	0.0	0.0	0.8243852061639481	0.0
5	3.006510598701488E-4	0.0	0.0	1.5283596628499014	0.0
6	3.006510598701488E-4	0.0	0.0	1.9507744019674604	0.0
7	3.006510598701488E-4	0.0	0.0	2.351842915834239	0.0
8	3.006510598701488E-4	0.0	0.0	2.9537463376942767	0.0
9	3.006510598701488E-4	0.0	0.0	3.226887825586307	0.0
10	3.006510598701488E-4	0.0	0.0	3.773922429020043	0.0
11	3.006510598701488E-4	0.0	0.0	4.48541316220275	0.0
12	4.5097658980522323E-4	0.0	0.0	5.103702066825711	0.0
13	4.5097658980522323E-4	0.0	0.0	5.3349027318658555	0.0
14	4.5097658980522323E-4	0.0	0.0	5.463280734430409	0.0
15	4.5097658980522323E-4	0.0	0.0	5.651337972379187	0.0
16	4.5097658980522323E-4	0.0	0.0	5.999341574178884	0.0
17	4.5097658980522323E-4	0.0	0.0	6.381168420213974	0.0
18	4.5097658980522323E-4	0.0	0.0	6.787348002098544	0.0
19	6.013021197402976E-4	0.0	0.0	7.094914036345707	0.0
20	6.013021197402976E-4	0.0	0.0	7.37466984755488	0.0
21	6.013021197402976E-4	0.0	0.0	7.712601638848927	0.0
22	6.013021197402976E-4	0.0	0.0	8.10134345926103	0.0
23	6.013021197402976E-4	0.0	0.0	8.429053114519492	0.0
24	6.013021197402976E-4	0.0	0.0	8.706704368309575	0.0
25	6.013021197402976E-4	0.0	0.0	8.964061675558423	0.0
26	7.51627649675372E-4	0.0	0.0	9.20428187239467	0.0
27	7.51627649675372E-4	0.0	0.0	9.49516177281904	0.0
28	7.51627649675372E-4	0.0	0.0	9.748911267349445	0.0
29	7.51627649675372E-4	0.0	0.0	10.029268380678358	0.0
30	7.51627649675372E-4	0.0	0.0	10.352468270038768	0.0
31	7.51627649675372E-4	0.0	0.0	10.604113207150084	0.0
32	7.51627649675372E-4	0.0	0.0	10.861320188868996	0.0
33	7.51627649675372E-4	0.0	0.0	11.136115257590312	0.0
34	7.51627649675372E-4	0.0	0.0	11.413766511380395	0.0
35	7.51627649675372E-4	0.0	0.0	11.70750259687353	0.0
36	7.51627649675372E-4	0.0	0.0	11.963055997763156	0.0
37	7.51627649675372E-4	0.0	0.0	12.255138502427005	0.0
38	9.019531796104465E-4	0.0	0.0	12.599383965978326	0.0
39	9.019531796104465E-4	0.0	0.0	13.143261733283426	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	20	7.03114E-4	45.000004	1
GGTATCG	20	7.03114E-4	45.000004	3
GCGATAC	25	3.8890274E-5	45.0	9
CGGTCTA	25	3.8890274E-5	45.0	31
CGTTTTT	25980	0.0	44.428406	1
CCATACG	60	3.6379788E-12	41.249996	2
CTAACGG	50	1.0804797E-9	40.5	2
GCTAACG	50	1.0804797E-9	40.5	1
GTTTTTT	28920	0.0	40.16857	2
CTAACAC	85	0.0	39.705883	28
AGGGCGA	240	0.0	39.374996	6
TTTAGCG	35	6.2443214E-6	38.571426	1
CGAACCC	60	1.546141E-10	37.499996	19
TTCACGG	30	1.1394051E-4	37.499996	2
TACGGGT	30	1.1394051E-4	37.499996	4
TAACGGG	120	0.0	37.499996	3
TATGCCA	30	1.1394051E-4	37.499996	10
CGTTTAC	30	1.1394051E-4	37.499996	33
GCATATC	60	1.546141E-10	37.499996	28
GACCGAT	165	0.0	36.818184	9
>>END_MODULE