SRR2935714.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935714.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211857
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15229	7.18833930434208	No Hit
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	598	0.2822658680147458	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	467	0.22043170629245196	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	328	0.154821412556583	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	304	0.14349301651585739	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	281	0.1326366369768287	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	254	0.11989219143101243	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	219	0.10337161387162096	Illumina PCR Primer Index 7 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCT	25	3.8800026E-5	45.000004	39
CATATGC	25	3.8800026E-5	45.000004	33
GCTTGTC	25	3.8800026E-5	45.000004	38
TGCGGGT	25	3.8800026E-5	45.000004	4
ATAAGGG	25	3.8800026E-5	45.000004	3
CAGTGCC	25	3.8800026E-5	45.000004	19
CAGTAGC	25	3.8800026E-5	45.000004	27
ACTATGC	35	1.2056626E-7	45.0	8
CTCACTA	20	7.020251E-4	45.0	45
GCGCGCG	20	7.020251E-4	45.0	1
GAACTAG	20	7.020251E-4	45.0	1
AATGGGC	20	7.020251E-4	45.0	4
CCTCGTG	35	1.2056626E-7	45.0	15
ACGGGTA	35	1.2056626E-7	45.0	5
GATGCTT	20	7.020251E-4	45.0	43
TGGGATC	40	6.7702786E-9	45.0	6
GCGTAGG	20	7.020251E-4	45.0	2
TGCACCA	35	1.2056626E-7	45.0	28
TAGTAGG	45	3.8198777E-10	45.0	2
TGATCTT	20	7.020251E-4	45.0	37