##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935711.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	213061
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30736737366294	33.0	31.0	34.0	30.0	34.0
2	32.49915751826941	34.0	31.0	34.0	30.0	34.0
3	32.37800911476056	34.0	31.0	34.0	30.0	34.0
4	36.02054341244995	37.0	35.0	37.0	35.0	37.0
5	36.03805013587658	37.0	35.0	37.0	35.0	37.0
6	36.04568644660449	37.0	35.0	37.0	35.0	37.0
7	36.169308320152446	37.0	36.0	37.0	35.0	37.0
8	35.73557338039341	37.0	35.0	37.0	35.0	37.0
9	37.642614087045494	39.0	38.0	39.0	35.0	39.0
10	37.58661603953797	39.0	37.0	39.0	35.0	39.0
11	37.57731353931503	39.0	37.0	39.0	35.0	39.0
12	37.44784826880565	39.0	37.0	39.0	35.0	39.0
13	37.36212164591361	39.0	37.0	39.0	34.0	39.0
14	38.7350664833076	40.0	38.0	41.0	35.0	41.0
15	38.76914592534532	40.0	38.0	41.0	35.0	41.0
16	38.920468785934546	41.0	38.0	41.0	35.0	41.0
17	38.797034652048005	41.0	38.0	41.0	35.0	41.0
18	38.24417420363182	39.0	38.0	40.0	35.0	41.0
19	37.62062977269421	37.0	37.0	40.0	35.0	41.0
20	36.70846846677712	36.0	35.0	40.0	34.0	41.0
21	36.818408812499705	36.0	35.0	40.0	33.0	41.0
22	36.75958997657948	36.0	35.0	40.0	33.0	41.0
23	36.685085491948314	36.0	35.0	40.0	33.0	41.0
24	36.587282515335986	36.0	35.0	40.0	33.0	41.0
25	36.61247717789741	36.0	35.0	40.0	33.0	41.0
26	36.415768254161954	35.0	35.0	40.0	33.0	41.0
27	36.233393253575265	35.0	35.0	40.0	33.0	41.0
28	36.111634696166824	35.0	35.0	40.0	33.0	41.0
29	35.890773064990775	35.0	35.0	40.0	32.0	41.0
30	35.44369922228845	35.0	35.0	40.0	30.0	41.0
31	34.85619611285031	35.0	35.0	40.0	25.0	41.0
32	33.78575619188871	35.0	34.0	40.0	18.0	41.0
33	32.72348294619851	35.0	33.0	40.0	12.0	41.0
34	31.767916230563078	35.0	30.0	40.0	8.0	41.0
35	31.295215924078082	35.0	27.0	40.0	7.0	41.0
36	30.79957852446013	35.0	24.0	40.0	7.0	41.0
37	30.627472883352656	35.0	23.0	40.0	7.0	41.0
38	30.491845058457436	35.0	23.0	40.0	7.0	41.0
39	30.384509600536937	35.0	23.0	40.0	7.0	41.0
40	30.3302387579144	35.0	23.0	40.0	7.0	41.0
41	30.097244451119632	35.0	22.0	40.0	7.0	41.0
42	29.984835328849485	35.0	21.0	40.0	7.0	41.0
43	29.740158921623387	35.0	21.0	40.0	7.0	41.0
44	29.79802497876195	35.0	21.0	40.0	7.0	41.0
45	29.777138941429918	35.0	20.0	40.0	7.0	41.0
46	29.778110494177724	35.0	20.0	40.0	7.0	41.0
47	29.706149881958687	35.0	20.0	40.0	7.0	41.0
48	29.72908697509164	35.0	21.0	40.0	7.0	41.0
49	29.743519461562652	35.0	20.0	40.0	7.0	41.0
50	29.54142710303622	35.0	20.0	40.0	7.0	41.0
51	28.958786450828637	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	1.0
11	5.0
12	5.0
13	1.0
14	7.0
15	9.0
16	22.0
17	45.0
18	98.0
19	207.0
20	416.0
21	805.0
22	1308.0
23	2103.0
24	3458.0
25	6148.0
26	10053.0
27	12697.0
28	11429.0
29	8878.0
30	7133.0
31	6446.0
32	6276.0
33	6830.0
34	10732.0
35	16489.0
36	14948.0
37	14755.0
38	26698.0
39	45013.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.141968731959395	12.230300242653511	13.138490854731744	42.48924017065535
2	53.72780565190251	21.67829870318829	13.32482246868268	11.269073176226527
3	18.807759280206138	22.11995625665889	49.39805971059931	9.674224752535658
4	17.740928654235173	14.627266369725103	56.49743500687596	11.134369969163762
5	20.889322776106372	17.25984577186815	48.48939974936755	13.361431702657924
6	15.269336011752502	25.64054425727843	49.70407535870009	9.386044372268975
7	46.49748194179132	3.8402147741726544	45.899531120195626	3.7627721638404026
8	45.02231755225029	9.339109456916095	41.070397679537784	4.568175311295826
9	41.66318566044466	4.939430491737109	41.486241029564304	11.911142818253927
10	28.40172532748837	14.903713021153568	46.28768287016394	10.406878781194118
11	23.00233266529304	14.900896926232393	49.32859603587705	12.768174372597517
12	18.79555620221439	12.957322081469627	52.29629073363966	15.950830982676322
13	17.461196558732006	15.941913348759277	55.05653310554255	11.540356986966174
14	13.149285885262907	22.13168998549711	50.818310249177465	13.900713880062519
15	10.798785324390668	15.962564711514544	59.293817263600566	13.944832700494224
16	12.173039645923	15.940505301298687	53.510966342972196	18.375488709806113
17	12.012991584569676	17.54896485044189	53.498763264980454	16.93928030000798
18	12.723116853858754	17.05145474770136	53.55086102102214	16.674567377417734
19	14.139143250055147	17.494520348632552	51.242132534813976	17.124203866498327
20	16.885305147352167	17.225113934507018	53.68931902131314	12.20026189682767
21	14.823454315900142	20.95690905421452	51.77672122068328	12.44291540920206
22	12.379553273475672	15.57582100900681	53.809941753770055	18.23468396374747
23	14.137265853441033	19.470949634142336	51.762640746077416	14.629143766339217
24	14.85959420072186	17.05239344600842	51.40593538939553	16.682076963874195
25	12.311966995367523	21.329572282116388	50.9670939308461	15.391366791669991
26	12.629247023152992	16.564739675491996	54.38583316514989	16.420180136205122
27	17.1237345173448	18.18399425516636	50.316106654901645	14.376164572587195
28	11.394389400218717	18.838266975185512	55.05465570892842	14.712687915667344
29	14.423099487940075	16.021702704859173	54.41915695505043	15.136040852150323
30	16.474624638014465	19.579838637761014	50.65028325221416	13.295253472010362
31	17.269702104092254	17.217604348050557	48.40210080681119	17.11059274104599
32	19.929034407986446	21.35679453302106	45.464444454874425	13.24972660411807
33	20.116774069397966	18.792270758139686	43.97332219411342	17.117632978348922
34	18.617672873026976	20.162770286443788	44.11365759101854	17.105899249510703
35	21.696133971022384	20.133670638925004	40.193653460745985	17.97654192930663
36	19.51131366134581	22.754046963076302	43.38241160982066	14.352227765757226
37	19.868488367181232	24.404747936037097	40.90518677749564	14.821576919286025
38	19.284618020191402	23.87109794847485	38.37868028405011	18.46560374728364
39	21.040922552696177	24.817775191142445	38.17216665649744	15.969135599663947
40	21.48633489939501	21.993231985206116	39.01652578369575	17.50390733170313
41	19.087022026555776	23.463233534058322	37.86051881855431	19.589225620831595
42	19.9266876622188	22.66158517983113	40.047216524845	17.364510633105073
43	19.787290963620745	22.5991617424118	39.061583302434514	18.55196399153294
44	19.012864860298222	22.655483640835254	39.20332674680021	19.12832475206631
45	17.77612984074983	21.665626276043014	38.84005050196892	21.718193381238237
46	21.471785075635616	23.829325873810784	38.2857491516514	16.413139898902195
47	16.652038618048355	21.754333266059955	44.25117689300247	17.342451222889217
48	17.86342878330619	21.62995574037482	40.17628754206542	20.33032793425357
49	17.364510633105073	19.095470311319293	45.371982671629254	18.168036383946383
50	17.891589732517918	19.284618020191402	42.61408704549402	20.20970520179667
51	17.245765297262285	19.596735207288056	39.56613364247797	23.591365852971684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	163.5
2	217.0
3	12204.0
4	24191.0
5	15925.5
6	7660.0
7	7206.5
8	6753.0
9	6552.5
10	6352.0
11	6145.0
12	5938.0
13	5582.5
14	5227.0
15	4783.5
16	4340.0
17	3918.5
18	3497.0
19	3232.5
20	2968.0
21	2617.0
22	2266.0
23	2136.5
24	2007.0
25	1989.5
26	1888.5
27	1805.0
28	1961.0
29	2117.0
30	2225.0
31	2333.0
32	2334.5
33	2336.0
34	2479.0
35	2622.0
36	2965.0
37	3308.0
38	3545.0
39	3782.0
40	4669.5
41	5557.0
42	6474.0
43	7391.0
44	8272.0
45	9153.0
46	15858.0
47	22563.0
48	19297.0
49	16031.0
50	15469.5
51	14908.0
52	12451.5
53	9995.0
54	9049.5
55	8104.0
56	7247.5
57	6391.0
58	5813.5
59	5236.0
60	4948.5
61	4661.0
62	4270.5
63	3880.0
64	3315.0
65	2750.0
66	2139.5
67	1529.0
68	1331.0
69	1133.0
70	884.0
71	635.0
72	618.5
73	602.0
74	474.0
75	295.5
76	245.0
77	158.5
78	72.0
79	65.0
80	58.0
81	37.0
82	16.0
83	9.0
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	213061.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.10427060794796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.09482914578211	35.7662828955088
2	7.808958273473167	6.888168177188693
3	2.630654790409603	3.4806933225695924
4	1.3706647937085636	2.4180868389803862
5	0.9971373538081708	2.1989007842824355
6	0.8013280975640903	2.1205194756431256
7	0.7108727346252487	2.1946766419006765
8	0.5374112739307644	1.8961705802563584
9	0.4927156828315721	1.9557779227545165
>10	3.4617799487064884	24.331529468086604
>50	0.058529940725132755	1.8281149529946823
>100	0.02660451851142398	2.446717137345643
>500	0.0031925422213708774	0.9668592562693314
>1k	0.004256722961827837	4.485100511121228
>5k	0.0	0.0
>10k+	0.0010641807404569593	7.0224020350979295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14962	7.0224020350979295	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	2997	1.4066394131258184	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	2861	1.3428079282459013	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	2541	1.192616199116685	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	1157	0.5430369706328234	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTAGGGTT	736	0.34544097699719795	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	713	0.3346459464660355	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT	611	0.28677233280609776	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	477	0.22387954623323839	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTA	449	0.2107377699344319	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC	405	0.19008640717916467	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	381	0.17882202749447343	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	292	0.13704995283041005	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	263	0.12343882737807482	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCGATCGAATGC	240	0.11264379684691238	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	235	0.11029705107926838	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	233	0.10935835277221077	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2304504343826416	0.0
2	0.0	0.0	0.0	1.322625914644163	0.0
3	0.0	0.0	0.0	1.7497336443553724	0.0
4	4.6934915352880163E-4	0.0	0.0	2.358010147328699	0.0
5	4.6934915352880163E-4	0.0	0.0	4.739487752333838	0.0
6	4.6934915352880163E-4	0.0	0.0	5.346356207846579	0.0
7	4.6934915352880163E-4	0.0	0.0	6.211366697800161	0.0
8	4.6934915352880163E-4	0.0	0.0	7.3068276221363835	0.0
9	4.6934915352880163E-4	0.0	0.0	7.633025283838901	0.0
10	4.6934915352880163E-4	0.0	0.0	9.76809458324142	0.0
11	4.6934915352880163E-4	0.0	0.0	10.841965446515317	0.0
12	4.6934915352880163E-4	0.0	0.0	13.241278319354551	0.0
13	4.6934915352880163E-4	0.0	0.0	13.713443567804525	0.0
14	9.386983070576033E-4	0.0	0.0	13.904468673290747	0.0
15	9.386983070576033E-4	0.0	0.0	14.406202918413037	0.0
16	9.386983070576033E-4	0.0	0.0	15.040293624830447	0.0
17	9.386983070576033E-4	0.0	0.0	15.70536137538076	0.0
18	9.386983070576033E-4	0.0	0.0	16.396712678528683	0.0
19	0.001408047460586405	0.0	0.0	17.186157954764127	0.0
20	0.0018773966141152065	0.0	0.0	17.66301669474939	0.0
21	0.0018773966141152065	0.0	0.0	18.115938627904686	0.0
22	0.0018773966141152065	0.0	0.0	18.662261042612208	0.0
23	0.002346745767644008	0.0	0.0	19.16305658942744	0.0
24	0.002346745767644008	0.0	0.0	19.529148929179907	0.0
25	0.002346745767644008	0.0	0.0	19.85957073326418	0.0
26	0.00281609492117281	0.0	0.0	20.186237744120227	0.0
27	0.00281609492117281	0.0	0.0	20.563125114403856	0.0
28	0.00281609492117281	0.0	0.0	20.878058396421682	0.0
29	0.00281609492117281	0.0	0.0	21.20801085135243	0.0
30	0.00281609492117281	0.0	0.0	21.577388635179595	0.0
31	0.00281609492117281	0.0	0.0	21.90968783587799	0.0
32	0.0032854440747016114	0.0	0.0	22.2537207654146	0.0
33	0.0032854440747016114	0.0	0.0	22.571939491507127	0.0
34	0.0032854440747016114	0.0	0.0	22.90329999389846	0.0
35	0.0032854440747016114	0.0	0.0	23.25484250989153	0.0
36	0.0032854440747016114	0.0	0.0	23.57869342582641	0.0
37	0.0032854440747016114	0.0	0.0	23.893157358690704	0.0
38	0.0032854440747016114	0.0	0.0	24.213253481397345	0.0
39	0.0032854440747016114	0.0	0.0	24.51739173288401	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	45	3.8198777E-10	45.000004	31
CGGGCTC	45	3.8198777E-10	45.000004	6
GTACTGG	30	2.1571523E-6	45.000004	2
ACCCGTC	30	2.1571523E-6	45.000004	39
TGATCCG	35	1.205699E-7	45.0	4
TCGTTCA	25	3.8800776E-5	45.0	16
AGATACA	20	7.0203404E-4	45.0	21
TCATTAC	20	7.0203404E-4	45.0	29
CGCGACC	20	7.0203404E-4	45.0	10
CCGTCTA	20	7.0203404E-4	45.0	41
ATCCGGC	35	1.205699E-7	45.0	6
AACGGGA	20	7.0203404E-4	45.0	4
CCCGTCT	25	3.8800776E-5	45.0	40
CCCGTAG	20	7.0203404E-4	45.0	41
TAATTGC	20	7.0203404E-4	45.0	30
TGCGAGA	20	7.0203404E-4	45.0	12
CGAGCAC	55	1.8189894E-12	45.0	34
CGTAAGC	25	3.8800776E-5	45.0	43
TAGATAC	25	3.8800776E-5	45.0	20
CCGGTCC	20	7.0203404E-4	45.0	36
>>END_MODULE