##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935710.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335425
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35978832824029	33.0	31.0	34.0	30.0	34.0
2	32.56340761720206	34.0	31.0	34.0	31.0	34.0
3	32.4048923008124	34.0	31.0	34.0	30.0	34.0
4	36.03877767012	37.0	35.0	37.0	35.0	37.0
5	36.07383170604457	37.0	35.0	37.0	35.0	37.0
6	36.07524185734516	37.0	36.0	37.0	35.0	37.0
7	36.230549303122906	37.0	37.0	37.0	35.0	37.0
8	35.96727435343221	37.0	37.0	37.0	35.0	37.0
9	37.80139524483864	39.0	38.0	39.0	35.0	39.0
10	37.66171871506298	39.0	38.0	39.0	35.0	39.0
11	37.62740702094358	39.0	37.0	39.0	35.0	39.0
12	37.574673921144814	39.0	37.0	39.0	35.0	39.0
13	37.551598718044275	39.0	37.0	39.0	35.0	39.0
14	38.951482447641055	41.0	38.0	41.0	35.0	41.0
15	39.03469628083774	41.0	39.0	41.0	35.0	41.0
16	39.10597600059626	41.0	39.0	41.0	35.0	41.0
17	39.02400834761869	41.0	39.0	41.0	35.0	41.0
18	38.38061861817098	39.0	38.0	40.0	35.0	41.0
19	37.69561600954014	37.0	37.0	40.0	35.0	41.0
20	36.845554147723035	36.0	35.0	40.0	34.0	41.0
21	36.82244018782142	36.0	35.0	40.0	34.0	41.0
22	36.81644779011702	35.0	35.0	40.0	34.0	41.0
23	36.810252664530076	35.0	35.0	40.0	34.0	41.0
24	36.64594171573377	35.0	35.0	40.0	34.0	41.0
25	36.57498695684579	35.0	35.0	40.0	33.0	41.0
26	36.40214951181337	35.0	35.0	40.0	33.0	41.0
27	36.31105314153685	35.0	35.0	40.0	33.0	41.0
28	36.1892047402549	35.0	35.0	40.0	33.0	41.0
29	35.954228217932474	35.0	35.0	40.0	33.0	41.0
30	35.47911753745249	35.0	35.0	40.0	31.0	41.0
31	34.86656033390475	35.0	35.0	40.0	25.0	41.0
32	33.850270552284414	35.0	34.0	40.0	18.0	41.0
33	32.72028322277708	35.0	33.0	40.0	12.0	41.0
34	31.750656629648955	35.0	31.0	40.0	8.0	41.0
35	31.187171498844748	35.0	27.0	40.0	7.0	41.0
36	30.849310576134755	35.0	24.0	40.0	7.0	41.0
37	30.67901617351122	35.0	23.0	40.0	7.0	41.0
38	30.498248490720727	35.0	23.0	40.0	7.0	41.0
39	30.349479019154803	35.0	23.0	40.0	7.0	41.0
40	30.195185212789745	35.0	22.0	40.0	7.0	41.0
41	30.018814936274875	35.0	21.0	40.0	7.0	41.0
42	29.870594022508758	35.0	21.0	40.0	7.0	41.0
43	29.71098159051949	35.0	20.0	40.0	7.0	41.0
44	29.59407915331296	35.0	19.0	40.0	7.0	41.0
45	29.511717969739884	35.0	20.0	40.0	7.0	41.0
46	29.459098159051948	35.0	20.0	40.0	7.0	41.0
47	29.476028918536187	35.0	20.0	40.0	7.0	41.0
48	29.462949988820153	35.0	20.0	40.0	7.0	41.0
49	29.43796079600507	35.0	20.0	40.0	7.0	41.0
50	29.320986807781175	35.0	20.0	39.0	7.0	41.0
51	28.713617052992472	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	2.0
9	5.0
10	3.0
11	4.0
12	5.0
13	8.0
14	7.0
15	12.0
16	37.0
17	84.0
18	158.0
19	308.0
20	592.0
21	929.0
22	1605.0
23	2649.0
24	4868.0
25	9847.0
26	16742.0
27	20626.0
28	19189.0
29	15092.0
30	11405.0
31	9765.0
32	9338.0
33	10257.0
34	15856.0
35	24700.0
36	26019.0
37	22589.0
38	39857.0
39	72800.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.849519266602073	12.666020719982113	14.224044123127374	46.26041589028844
2	58.6682566892748	15.712603413579787	14.682268763508983	10.936871133636432
3	18.887381679958263	17.160020869046733	53.21517477826638	10.737422672728627
4	17.94976522322427	14.972944771558472	54.675411791011406	12.401878214205858
5	17.24796899455914	17.329656406052024	52.474919877767014	12.947454721621824
6	15.848848475814265	21.813222031750765	52.455243347991356	9.882686144443616
7	48.6159350078259	3.8333457553849595	44.01937840053664	3.5313408362525154
8	48.379518521278975	4.679138406499217	42.98964000894387	3.951703063277931
9	45.005888052470745	4.6257732727137215	44.14190951777596	6.2264291570395764
10	26.27263918908847	14.611015875381977	48.70030558247	10.416039353059551
11	18.79943355444585	15.373332339569204	52.11597227398077	13.711261832004173
12	16.931057613475442	12.992472236714617	55.97913095326824	14.0973391965417
13	15.439815159871802	14.438100916747409	56.895878363270484	13.226205560110307
14	11.848252217336215	17.90713274204368	55.59275545949169	14.651859581128418
15	11.566221957218454	15.126779458895431	59.169411940076024	14.13758664381009
16	13.396139226354626	15.257956324066482	56.44898263397182	14.896921815607064
17	13.348438548110606	15.232615338749348	54.99888201535366	16.42006409778639
18	14.013863009614669	15.589774167101439	55.40582842662294	14.990534396660953
19	14.27740925691287	16.740553029738393	53.56577476335992	15.416262949988822
20	16.110009689200268	15.920995751658342	54.40500857121562	13.563985987925767
21	14.785123350972645	16.894685846314378	55.39658642021317	12.923604382499814
22	14.38741894611314	14.450622344786465	54.783334575538504	16.3786241335619
23	14.495341730640233	16.060520235522098	54.63426995602594	14.80986807781173
24	14.42647387642543	15.216814489081015	54.83043899530446	15.52627263918909
25	13.460535141984051	17.353506745174034	53.39733174331073	15.788626369531192
26	13.287620183349484	17.395542967876573	53.854960125214276	15.461876723559664
27	14.125959603488111	16.525005589923232	54.19005738987851	15.158977416710142
28	11.841097115599613	17.073265260490423	55.32026533502273	15.765372288887233
29	13.261682939554298	16.22121189535664	54.22225534769323	16.29484981739584
30	14.092867257956323	16.948050980099875	52.86606543936797	16.09301632257584
31	14.907952597450997	17.59916523813073	50.76335991652381	16.72952224789446
32	15.305955131549526	19.13244391443691	48.708653201162704	16.85294775285086
33	16.30051427293732	18.757695460982333	46.57673101289409	18.365059253186256
34	15.39241261086681	20.530968174703734	45.44533055079377	18.631288663635686
35	16.032197957814713	21.839457404784973	42.96847283297309	19.15987180442722
36	16.990683461280465	23.691436237609004	42.024595662219575	17.29328463889096
37	16.175598121785796	22.988149362748754	42.37728255198628	18.45896996347917
38	16.91406424685101	23.33874934784229	40.81478721025565	18.932399195051055
39	17.883282402921665	22.337631363195946	40.409629574420514	19.369456659461875
40	17.98375195647313	22.4783483640158	40.47611239472311	19.061787284787957
41	15.81992993962883	23.90966684057539	39.8172467764776	20.45315644331818
42	16.560184840128194	23.751956473131102	40.375940970410674	19.31191771633003
43	17.7127524781993	23.202206156368785	39.57576209286726	19.509279272564655
44	17.319221882686143	23.382872475218008	38.865320116270404	20.432585525825445
45	17.294179026608035	22.16471640456138	38.63844376537229	21.9026608034583
46	18.132518446746666	22.952970112543788	39.808004770067825	19.106506670641725
47	15.504807333979281	22.470298874562122	42.93150480733398	19.09338898412462
48	16.24059029589327	21.466199597525527	42.231497354103006	20.0617127524782
49	16.607885518372214	20.584631437728255	43.33129611686666	19.476186927032867
50	15.692926883804128	20.75039129462622	42.85727062681672	20.699411194752926
51	15.27584407840799	20.787359320265335	41.071178355817246	22.865618245509427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	247.0
2	362.0
3	22114.5
4	43867.0
5	28186.5
6	12506.0
7	12048.5
8	11591.0
9	11387.0
10	11183.0
11	10485.0
12	9787.0
13	9220.5
14	8654.0
15	7927.5
16	7201.0
17	6440.5
18	5680.0
19	5190.0
20	4700.0
21	4356.5
22	4013.0
23	3599.0
24	3185.0
25	3099.5
26	2830.5
27	2647.0
28	2784.5
29	2922.0
30	3100.0
31	3278.0
32	3489.5
33	3701.0
34	4189.0
35	4677.0
36	5061.5
37	5446.0
38	5954.0
39	6462.0
40	7440.0
41	8418.0
42	9542.0
43	10666.0
44	11784.5
45	12903.0
46	16979.5
47	21056.0
48	21467.0
49	21878.0
50	21904.0
51	21930.0
52	19072.0
53	16214.0
54	14719.0
55	13224.0
56	12216.5
57	11209.0
58	10725.5
59	10242.0
60	9559.0
61	8876.0
62	8102.0
63	7328.0
64	6356.5
65	5385.0
66	4573.5
67	3762.0
68	3218.5
69	2675.0
70	2229.5
71	1784.0
72	1617.5
73	1451.0
74	1051.5
75	534.0
76	416.0
77	267.5
78	119.0
79	108.0
80	97.0
81	82.0
82	67.0
83	61.5
84	56.0
85	30.5
86	5.0
87	3.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.131325158276184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18750902453418	35.829123595522
2	7.728128536446517	6.821051066137486
3	2.592181146801722	3.4318916717598023
4	1.3225453927134034	2.3346272304966114
5	0.8969474686836706	1.9791740195185905
6	0.6674316839080996	1.7672786798090492
7	0.594014364193565	1.835024873843904
8	0.5194070566523143	1.833769736530117
9	0.4811610980144154	1.911084918298863
>10	3.8977177720669465	28.10881572681996
>50	0.08301619054331326	2.376844979819192
>100	0.024496580816059647	2.1561272211120404
>500	0.0027218423128955167	0.7921815611829475
>1k	0.0020413817346716374	1.0083948758348513
>5k	0.0	0.0
>10k+	6.804605782238792E-4	7.814609843314573
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26023	7.75821718715063	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGC	1285	0.3830960721472758	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC	1041	0.3103525378251472	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCG	1032	0.30766937467392114	No Hit
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	835	0.248937914585973	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	760	0.2265782216590892	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	539	0.16069165983453826	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	504	0.15025713646865915	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	475	0.14161138853693078	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	461	0.1374375791905791	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	403	0.1201460833271223	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14369829321010658	0.0
2	0.0	0.0	0.0	0.7873593202653351	0.0
3	0.0	0.0	0.0	1.1030781843929343	0.0
4	0.0	0.0	0.0	1.5037638816426921	0.0
5	0.0	0.0	0.0	2.7815458001043454	0.0
6	0.0	0.0	0.0	3.2931355742714468	0.0
7	0.0	0.0	0.0	3.864649325482597	0.0
8	0.0	0.0	0.0	4.6901691883431464	0.0
9	0.0	0.0	0.0	4.946560333904748	0.0
10	0.0	0.0	0.0	5.861817097711858	0.0
11	0.0	0.0	0.0	6.884102258328985	0.0
12	0.0	0.0	0.0	8.03726615487814	0.0
13	0.0	0.0	0.0	8.372065290303347	0.0
14	0.0	0.0	0.0	8.526198106879333	0.0
15	0.0	0.0	0.0	8.820153536558099	0.0
16	0.0	0.0	0.0	9.331445181486174	0.0
17	0.0	0.0	0.0	9.907132742043675	0.0
18	0.0	0.0	0.0	10.574345978981889	0.0
19	0.0	0.0	0.0	11.050756502944026	0.0
20	0.0	0.0	0.0	11.459193560408437	0.0
21	0.0	0.0	0.0	11.929045241112021	0.0
22	0.0	0.0	0.0	12.410523962137587	0.0
23	0.0	0.0	0.0	12.854140269806962	0.0
24	0.0	0.0	0.0	13.249757769993293	0.0
25	0.0	0.0	0.0	13.565178504881866	0.0
26	0.0	0.0	0.0	13.861817097711858	0.0
27	0.0	0.0	0.0	14.18707609748826	0.0
28	0.0	0.0	0.0	14.491167921293881	0.0
29	0.0	0.0	0.0	14.802712976075128	0.0
30	0.0	0.0	0.0	15.1750763956175	0.0
31	0.0	0.0	0.0	15.506894238652455	0.0
32	2.981292390251174E-4	0.0	0.0	15.84616531266304	0.0
33	2.981292390251174E-4	0.0	0.0	16.13922635462473	0.0
34	2.981292390251174E-4	0.0	0.0	16.45792651114258	0.0
35	2.981292390251174E-4	0.0	0.0	16.800775136021464	0.0
36	2.981292390251174E-4	0.0	0.0	17.129313557427146	0.0
37	2.981292390251174E-4	0.0	0.0	17.449206230901094	0.0
38	2.981292390251174E-4	0.0	0.0	17.749720503838414	0.0
39	2.981292390251174E-4	0.0	0.0	18.080942088395318	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	35	1.208482E-7	45.000004	27
ACCGGTA	20	7.026135E-4	45.0	41
TTAGCGG	20	7.026135E-4	45.0	2
TGGCGTA	20	7.026135E-4	45.0	28
CGCGCGC	20	7.026135E-4	45.0	40
CGATTGT	25	3.8848797E-5	45.0	10
GTTGTAA	20	7.026135E-4	45.0	32
TCAGCCA	25	3.8848797E-5	45.0	41
AGACCTA	25	3.8848797E-5	45.0	32
AAATTCG	20	7.026135E-4	45.0	30
GCGATAT	20	7.026135E-4	45.0	9
CGTACCA	20	7.026135E-4	45.0	31
CGTACAT	20	7.026135E-4	45.0	35
TCGTACA	20	7.026135E-4	45.0	34
CCGGTAT	20	7.026135E-4	45.0	42
CGGTATT	20	7.026135E-4	45.0	43
TGGGACG	25	3.8848797E-5	45.0	6
CGCCCCG	20	7.026135E-4	45.0	25
ATATTAC	20	7.026135E-4	45.0	45
CTTACGG	25	3.8848797E-5	45.0	2
>>END_MODULE