##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935709.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	694943
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45731952116936	34.0	31.0	34.0	31.0	34.0
2	32.662673053761246	34.0	31.0	34.0	31.0	34.0
3	32.503703181412	34.0	31.0	34.0	31.0	34.0
4	36.10222708912817	37.0	35.0	37.0	35.0	37.0
5	36.13335482190626	37.0	35.0	37.0	35.0	37.0
6	36.153424093774596	37.0	37.0	37.0	35.0	37.0
7	36.30283203082843	37.0	37.0	37.0	35.0	37.0
8	36.10282857730778	37.0	37.0	37.0	35.0	37.0
9	37.914446220769186	39.0	39.0	39.0	35.0	39.0
10	37.771359665468964	39.0	38.0	39.0	35.0	39.0
11	37.741957254048174	39.0	38.0	39.0	35.0	39.0
12	37.71469314749555	39.0	38.0	39.0	35.0	39.0
13	37.75153501797989	39.0	38.0	39.0	35.0	39.0
14	39.17992266991681	41.0	39.0	41.0	35.0	41.0
15	39.27106683569732	41.0	39.0	41.0	35.0	41.0
16	39.30634167118742	41.0	39.0	41.0	35.0	41.0
17	39.25738945496249	41.0	39.0	41.0	35.0	41.0
18	38.609001025983424	39.0	39.0	41.0	35.0	41.0
19	37.93769273163411	38.0	37.0	41.0	35.0	41.0
20	37.15673515669631	37.0	35.0	41.0	34.0	41.0
21	37.11827013150719	37.0	35.0	41.0	34.0	41.0
22	37.10881036286429	37.0	35.0	41.0	34.0	41.0
23	37.10514819200999	37.0	35.0	41.0	34.0	41.0
24	36.95709000594293	37.0	35.0	41.0	34.0	41.0
25	36.878414488670295	37.0	35.0	41.0	34.0	41.0
26	36.710242422759855	37.0	35.0	41.0	33.0	41.0
27	36.63104168255526	36.0	35.0	40.0	33.0	41.0
28	36.483764855534915	36.0	35.0	40.0	33.0	41.0
29	36.220156185471325	36.0	35.0	40.0	33.0	41.0
30	35.720109419045876	36.0	35.0	40.0	31.0	41.0
31	35.05626648516497	36.0	35.0	40.0	25.0	41.0
32	34.032336465005045	36.0	34.0	40.0	18.0	41.0
33	32.866666762597795	36.0	33.0	40.0	12.0	41.0
34	31.879522493211674	36.0	30.0	40.0	8.0	41.0
35	31.28892297641677	36.0	24.0	40.0	7.0	41.0
36	30.907688832033706	36.0	23.0	40.0	7.0	41.0
37	30.723374435025608	36.0	22.0	40.0	7.0	41.0
38	30.558176713773648	36.0	22.0	40.0	7.0	41.0
39	30.423647694846917	35.0	21.0	40.0	7.0	41.0
40	30.30261042992015	35.0	21.0	40.0	7.0	41.0
41	30.12135959352062	35.0	20.0	40.0	7.0	41.0
42	30.00173539412585	35.0	20.0	40.0	7.0	41.0
43	29.892096186305928	35.0	18.0	40.0	7.0	41.0
44	29.784966536823884	35.0	18.0	40.0	7.0	41.0
45	29.724459416095996	35.0	20.0	40.0	7.0	41.0
46	29.66189601161534	35.0	20.0	40.0	7.0	41.0
47	29.659580713813938	35.0	20.0	40.0	7.0	41.0
48	29.58307947558289	35.0	20.0	40.0	7.0	41.0
49	29.5541145101109	35.0	20.0	40.0	7.0	41.0
50	29.44534443832084	35.0	20.0	40.0	7.0	41.0
51	28.822264559827207	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	6.0
9	18.0
10	13.0
11	12.0
12	10.0
13	13.0
14	20.0
15	41.0
16	92.0
17	170.0
18	333.0
19	681.0
20	1194.0
21	1891.0
22	2993.0
23	5412.0
24	10853.0
25	22758.0
26	38083.0
27	44204.0
28	38300.0
29	28535.0
30	21694.0
31	18450.0
32	16960.0
33	18367.0
34	27789.0
35	39105.0
36	43265.0
37	45195.0
38	86594.0
39	181689.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.55720397212433	13.663422755535345	15.47839175299269	45.30098151934763
2	58.08303702605825	15.588904413743284	15.561995732024064	10.766062828174396
3	20.37663520605287	15.530482356106903	53.227962581103775	10.864919856736451
4	18.148394904330285	16.62107539755059	53.326387919584775	11.904141778534354
5	16.737919512823353	18.88816780656831	51.886845395953344	12.487067284655
6	16.679353558493286	21.81531434952219	51.28765956344621	10.217672528538312
7	51.60480787632943	3.5638894125129688	41.34281516613593	3.488487545021678
8	52.59237088509418	3.2611307689983207	40.934436349456	3.2120619964515074
9	48.463830846558636	5.083438497833635	42.14115402270402	4.311576632903705
10	23.024622163256556	22.878423122471915	45.69957536085693	8.397379353414596
11	17.556835596588495	16.385660406680834	54.14429672649411	11.913207270236551
12	17.34804149405059	14.914748403825925	54.92579966990099	12.811410432222498
13	15.569046670014664	15.037204490152432	56.01394646755201	13.379802372280892
14	12.697588147517132	17.998742342897188	54.96940036808774	14.334269141497934
15	12.366194061959037	18.037738346886005	56.593130659636834	13.002936931518125
16	14.785816966283566	17.65943393918638	54.91659028150511	12.638158813024953
17	14.310094496958744	17.614825964143822	53.71936979004034	14.355709748857102
18	14.704803127738533	17.95960244221469	53.77879912453252	13.556795305514266
19	15.245135212528222	18.586560336603146	52.778429310029736	13.389875140838889
20	16.362061348916384	17.807503636988933	52.92232600371541	12.908109010379269
21	15.577680471635801	17.922764888631153	53.71059209172551	12.788962548007534
22	15.202829584584634	16.383501956275552	53.8071467731886	14.60652168595122
23	14.649834590750608	17.634683707872444	53.80642728972016	13.909054411656783
24	14.638754545336813	16.483797951774463	54.55785582414673	14.319591678741997
25	14.588390702546828	18.35013806887759	52.456676302948594	14.604794925626994
26	14.14705954301288	19.084011206674504	52.397822555231144	14.371106695081467
27	14.752289036654807	18.122493499466863	53.14479029215345	13.980427171724873
28	14.070362605278419	18.591165030801086	53.331855993944835	14.006616369975667
29	14.713724722747045	18.033997032850177	52.67151406662129	14.580764177781488
30	15.847486772296431	17.815274058447958	51.60567125649153	14.731567912764069
31	15.560125075006152	19.15452058658048	50.212751261614265	15.072603076799105
32	16.88555752054485	20.297348127832066	48.21172383922135	14.605370512401736
33	17.15924903193499	21.564214619040698	45.61093499754656	15.665601351477749
34	16.471710629504866	22.806043085548023	44.49990862559951	16.222337659347602
35	17.827793070798613	23.525382657282684	41.61147029324707	17.035353978671633
36	17.78462406269291	26.06141798679892	39.44539336319669	16.70856458731148
37	18.55648592762284	25.628288938805056	40.13365124909525	15.681573884476856
38	18.3761833704347	25.244804250132745	39.15256359154636	17.226448787886202
39	18.87046851324497	24.767067227096323	38.73655825010109	17.625906009557617
40	19.12660462800546	24.2963811420505	39.793911155303384	16.783103074640653
41	17.77901209163917	25.560513596079105	39.12824505031348	17.53222926196825
42	18.564688039162924	25.930328098851273	37.85389593103319	17.65108793095261
43	18.718513604712903	25.14968853560652	38.28011793773015	17.851679921950435
44	18.39791177118123	25.097454035798624	37.5525762544554	18.952057938564746
45	18.44784392389016	23.789145296808513	37.798927394045265	19.964083385256057
46	18.2817871393769	24.66749071506584	37.91274967875063	19.13797246680663
47	17.666197083789605	23.909155139342364	39.97766723313998	18.44698054372805
48	17.718143790210135	23.078439526694993	40.098396559142266	19.105020123952613
49	17.762607868559005	22.877271948922427	39.96126301005982	19.398857172458747
50	16.795046500216564	22.520408148581968	40.78809341197767	19.89645193922379
51	16.743819277264464	22.18009246801536	39.82312218412157	21.252966070598596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	194.0
1	390.0
2	586.0
3	37828.0
4	75070.0
5	50237.5
6	25405.0
7	24443.5
8	23482.0
9	22801.5
10	22121.0
11	21320.0
12	20519.0
13	19483.0
14	18447.0
15	17257.0
16	16067.0
17	15006.5
18	13946.0
19	12701.5
20	11457.0
21	10554.5
22	9652.0
23	8932.0
24	8212.0
25	7684.5
26	7223.5
27	7290.0
28	7670.0
29	8050.0
30	8740.0
31	9430.0
32	10504.0
33	11578.0
34	12560.5
35	13543.0
36	14874.0
37	16205.0
38	17659.5
39	19114.0
40	20537.0
41	21960.0
42	23592.0
43	25224.0
44	28000.0
45	30776.0
46	36032.5
47	41289.0
48	43573.0
49	45857.0
50	44141.5
51	42426.0
52	37295.0
53	32164.0
54	28282.5
55	24401.0
56	22173.0
57	19945.0
58	19067.5
59	18190.0
60	17495.0
61	16800.0
62	15287.0
63	13774.0
64	11603.0
65	9432.0
66	7548.5
67	5665.0
68	4686.5
69	3708.0
70	2944.5
71	2181.0
72	1915.5
73	1650.0
74	1321.0
75	762.0
76	532.0
77	391.5
78	251.0
79	169.5
80	88.0
81	68.0
82	48.0
83	38.5
84	29.0
85	29.5
86	30.0
87	18.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	694943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.046216107414736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1019940135009	35.63245516859588
2	7.6347298157727215	6.878313784060415
3	3.128181266751761	4.227381880957987
4	1.9097144809220221	3.4410164484429107
5	1.3729825095646602	3.0923833418775155
6	1.1444473480086128	3.093181353717219
7	0.9013715120582194	2.8422363147669216
8	0.7660665929875885	2.7606721040311872
9	0.6408856992974521	2.5982528139634185
>10	3.284233345358471	22.108310916852844
>50	0.07167892327281264	2.281199745757828
>100	0.038571611033594536	2.8146135123992706
>500	0.0035357310114121623	1.1516763145028597
>1k	0.0012857203677862409	0.8234326377392209
>5k	0.0	0.0
>10k+	3.214300919465602E-4	6.254873662334553
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43199	6.216193270527223	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	1598	0.22994691650969934	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	1559	0.22433494545595825	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	1447	0.20821851576316333	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	1083	0.15584011926157973	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	922	0.13267275157818698	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	893	0.12849974746130258	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	855	0.12303167310124714	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	783	0.11267111115587897	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	755	0.10864200373268022	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	748	0.10763472687688054	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09972040872416875	0.0
2	0.0	0.0	0.0	0.4427701264708041	0.0
3	0.0	0.0	0.0	0.8301400258726255	0.0
4	0.0	0.0	0.0	1.1950620410594825	0.0
5	0.0	0.0	0.0	2.0768609799652635	0.0
6	0.0	0.0	0.0	2.911317906648459	0.0
7	0.0	0.0	0.0	3.5217276812630676	0.0
8	0.0	0.0	0.0	4.349709256730408	0.0
9	0.0	0.0	0.0	4.7727655361662755	0.0
10	0.0	0.0	0.0	5.412674132986447	0.0
11	0.0	0.0	0.0	6.155468865791871	0.0
12	0.0	0.0	0.0	6.803291780764753	0.0
13	0.0	0.0	0.0	7.098711692901432	0.0
14	0.0	0.0	0.0	7.254551812163012	0.0
15	0.0	0.0	0.0	7.45097079904395	0.0
16	0.0	0.0	0.0	7.80020807461907	0.0
17	0.0	0.0	0.0	8.220674213568595	0.0
18	0.0	0.0	0.0	8.667329550768912	0.0
19	0.0	0.0	0.0	8.967210260409846	0.0
20	0.0	0.0	0.0	9.258888858510698	0.0
21	0.0	0.0	0.0	9.623810873697556	0.0
22	0.0	0.0	0.0	10.033196967233284	0.0
23	1.4389669368566917E-4	0.0	0.0	10.420998556716162	0.0
24	1.4389669368566917E-4	0.0	0.0	10.715698985384412	0.0
25	1.4389669368566917E-4	0.0	0.0	10.990973360405098	0.0
26	1.4389669368566917E-4	0.0	0.0	11.264952665182612	0.0
27	1.4389669368566917E-4	0.0	0.0	11.545839011257039	0.0
28	1.4389669368566917E-4	0.0	0.0	11.827732634187264	0.0
29	2.8779338737133835E-4	0.0	0.0	12.127757240521884	0.0
30	2.8779338737133835E-4	0.0	0.0	12.472389821899062	0.0
31	2.8779338737133835E-4	0.0	0.0	12.789250341394906	0.0
32	2.8779338737133835E-4	0.0	0.0	13.091001708053755	0.0
33	2.8779338737133835E-4	0.0	0.0	13.379946268974578	0.0
34	4.3169008105700755E-4	0.0	0.0	13.669322519976458	0.0
35	4.3169008105700755E-4	0.0	0.0	13.975822477526934	0.0
36	4.3169008105700755E-4	0.0	0.0	14.28462478217638	0.0
37	4.3169008105700755E-4	0.0	0.0	14.600478024816423	0.0
38	4.3169008105700755E-4	0.0	0.0	14.927986899645006	0.0
39	4.3169008105700755E-4	0.0	0.0	15.277368071913811	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.0313603E-4	45.0	1
CGAACGG	20	7.0313603E-4	45.0	2
CATTACG	20	7.0313603E-4	45.0	36
TGCGTCC	20	7.0313603E-4	45.0	42
GCACGAG	25	3.889209E-5	44.999996	1
CGCCTCT	25	3.889209E-5	44.999996	38
CGTTTTT	25200	0.0	44.580357	1
GTGCTAG	55	6.002665E-11	40.909092	1
GCGTAAG	50	1.0804797E-9	40.499996	1
GTTTTTT	27865	0.0	40.429752	2
ATACCGG	45	1.9264917E-8	40.0	2
ACCTGGC	40	3.4555887E-7	39.375	45
TCGCCTC	35	6.2447234E-6	38.57143	37
ATAGCGG	70	0.0	38.57143	2
TTCGTGG	35	6.2447234E-6	38.57143	2
CGTTTCT	210	0.0	38.57143	1
AACACGG	35	6.2447234E-6	38.57143	2
CGGACGG	35	6.2447234E-6	38.57143	2
GGCGATA	135	0.0	38.333332	8
ACAGCGG	65	9.094947E-12	38.07692	2
>>END_MODULE