##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935705.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375950
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44132198430643	34.0	31.0	34.0	31.0	34.0
2	32.64629605000665	34.0	31.0	34.0	31.0	34.0
3	32.48145232078734	34.0	31.0	34.0	31.0	34.0
4	36.08608591568028	37.0	35.0	37.0	35.0	37.0
5	36.11273839606331	37.0	35.0	37.0	35.0	37.0
6	36.11935895730815	37.0	36.0	37.0	35.0	37.0
7	36.24497140577204	37.0	37.0	37.0	35.0	37.0
8	36.00073148025003	37.0	37.0	37.0	35.0	37.0
9	37.81785077802899	39.0	38.0	39.0	35.0	39.0
10	37.70704082989759	39.0	38.0	39.0	35.0	39.0
11	37.69186061976327	39.0	38.0	39.0	35.0	39.0
12	37.6021944407501	39.0	37.0	39.0	35.0	39.0
13	37.58178747173827	39.0	37.0	39.0	35.0	39.0
14	38.98428780422928	41.0	39.0	41.0	35.0	41.0
15	39.09156536773507	41.0	39.0	41.0	35.0	41.0
16	39.124043090836544	41.0	39.0	41.0	35.0	41.0
17	39.062053464556456	41.0	39.0	41.0	35.0	41.0
18	38.38398989227291	39.0	38.0	40.0	35.0	41.0
19	37.68272642638649	37.0	37.0	40.0	35.0	41.0
20	36.84658066232212	35.0	35.0	40.0	34.0	41.0
21	36.790833887485036	35.0	35.0	40.0	34.0	41.0
22	36.76946402447134	35.0	35.0	40.0	34.0	41.0
23	36.789652879372255	35.0	35.0	40.0	34.0	41.0
24	36.628144700093095	35.0	35.0	40.0	34.0	41.0
25	36.53082324777231	35.0	35.0	40.0	33.0	41.0
26	36.36200558584918	35.0	35.0	40.0	33.0	41.0
27	36.28093363479186	35.0	35.0	40.0	33.0	41.0
28	36.1106476925123	35.0	35.0	40.0	33.0	41.0
29	35.825793323580264	35.0	35.0	40.0	33.0	41.0
30	35.28788934698763	35.0	35.0	40.0	30.0	41.0
31	34.5926213592233	35.0	35.0	40.0	23.0	41.0
32	33.529527862747706	35.0	33.0	40.0	17.0	41.0
33	32.314318393403376	35.0	32.0	40.0	12.0	41.0
34	31.29082324777231	35.0	27.0	40.0	8.0	41.0
35	30.68533847586115	35.0	23.0	40.0	7.0	41.0
36	30.323383428647425	35.0	22.0	40.0	7.0	41.0
37	30.102830163585583	35.0	21.0	40.0	7.0	41.0
38	29.915039233940682	35.0	21.0	40.0	7.0	41.0
39	29.806016757547546	35.0	20.0	40.0	7.0	41.0
40	29.685998138050273	35.0	20.0	40.0	7.0	41.0
41	29.48426652480383	35.0	18.0	40.0	7.0	41.0
42	29.34132730416279	35.0	18.0	40.0	7.0	41.0
43	29.19949195371725	35.0	18.0	40.0	7.0	41.0
44	29.081170368400052	35.0	18.0	40.0	7.0	41.0
45	29.051791461630536	35.0	18.0	40.0	7.0	41.0
46	28.999521212927252	35.0	18.0	40.0	7.0	41.0
47	29.032025535310545	35.0	20.0	40.0	7.0	41.0
48	28.967458438622156	35.0	20.0	40.0	7.0	41.0
49	28.915696236201622	35.0	20.0	39.0	7.0	41.0
50	28.790969543822317	35.0	18.0	39.0	7.0	41.0
51	28.144420800638382	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	11.0
10	11.0
11	6.0
12	9.0
13	6.0
14	21.0
15	24.0
16	63.0
17	118.0
18	226.0
19	451.0
20	676.0
21	1141.0
22	1704.0
23	2973.0
24	5651.0
25	11704.0
26	20192.0
27	24893.0
28	22712.0
29	17771.0
30	13769.0
31	11557.0
32	10531.0
33	11332.0
34	18279.0
35	25925.0
36	25510.0
37	24812.0
38	44509.0
39	79277.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.30642372655938	12.494214656204282	13.85503391408432	47.34432770315202
2	60.325841202287535	14.555127011570686	14.187258944008512	10.931772842133263
3	20.49049075674957	14.921133129405506	53.5940949594361	10.99428115440883
4	18.310413618832293	15.858757813539034	53.59010506716319	12.240723500465487
5	16.413352839473333	18.084851709003857	52.63253092166511	12.869264529857693
6	16.281952387285543	21.36986301369863	52.90650352440485	9.441681074610987
7	48.374251895198825	4.2117302832823516	43.88695305226759	3.52706476925123
8	48.249235270647695	4.333023008378774	43.799175422263595	3.6185662987099345
9	44.50565234738662	5.562175821252826	45.21292725096423	4.719244580396329
10	25.59861683734539	15.803431307354701	49.368799042425856	9.229152812874053
11	20.194972735736137	15.675222768985236	52.500066498204546	11.629737997074079
12	19.5153610852507	13.465886421066632	54.90730150285942	12.111450990823249
13	16.565500731480252	14.419736667109989	55.42119962761005	13.593562973799708
14	12.730682271578667	17.108126080595824	55.72788934698764	14.433302300837877
15	12.972203750498737	16.011703684000533	58.48703285011305	12.529059715387683
16	14.65327836148424	15.39992020215454	57.14456709668839	12.80223433967283
17	13.901848650086448	15.3012368666046	55.52706476925123	15.26984971405772
18	14.748769783215854	16.20747439819125	55.233940683601546	13.809815134991354
19	16.21226226891874	16.703816996941082	54.154275834552465	12.92964489958771
20	17.907700492086715	15.546482244979384	54.423194573746514	12.122622689187391
21	16.485436893203882	16.438622157201756	54.474265194839745	12.601675754754622
22	14.69690118366804	16.091767522276896	54.68759143503126	14.523739859023808
23	15.349115573879505	16.30828567628674	54.829897592765	13.51270115706876
24	15.832158531719642	14.631998936028728	55.11184998005054	14.42399255220109
25	14.674025801303364	16.020747439819125	54.06064636254821	15.2445803963293
26	13.999202021545418	16.977789599680808	54.55539300438888	14.467615374384893
27	14.839207341401783	16.596621891208937	54.499002526931775	14.065168240457506
28	13.605266657800238	17.752892671897857	54.47320122356696	14.168639446734938
29	15.097220375049874	16.818991887219045	54.30668971937757	13.777098018353504
30	15.660859156802767	16.990291262135923	52.91341933767788	14.43543024338343
31	15.992286208272377	18.910493416677753	50.471871259475996	14.62534911557388
32	16.821651815400983	20.080063838276367	49.08950658332225	14.0087777630004
33	17.465354435430243	21.490623753158662	46.130602473733205	14.913419337677883
34	17.104136188322915	22.69397526266791	44.50059848384094	15.70129006516824
35	17.615108392073413	23.439021146429045	42.559116903843595	16.386753557653943
36	18.466019417475728	26.290730150285942	39.56031387152547	15.682936560712859
37	19.312142572150552	25.97872057454449	39.69464024471339	15.014496608591568
38	17.56909163452587	26.331959037106	39.501263465886424	16.59768586248171
39	18.984173427317465	24.742119962761006	39.34219976060646	16.931506849315067
40	19.087644633594895	24.256151083920734	39.998138050272644	16.65806623221173
41	17.602872722436494	25.4714722702487	39.31560047878707	17.61005452852773
42	18.06942412554861	25.793589573081526	38.43809017156537	17.698896129804496
43	18.565766724298445	25.23793057587445	38.37611384492619	17.820188854900916
44	17.448596887884026	25.72549541162389	38.30668971937758	18.51921798111451
45	18.25269317728421	24.096821385822583	38.65567229684799	18.99481314004522
46	17.736933102806223	25.057587445138978	39.463492485702886	17.74198696635191
47	17.097220375049872	24.126346588642107	41.422795584519214	17.3536374517888
48	17.257614044420798	22.845059183402046	41.83082856762867	18.066498204548477
49	17.17861417741721	22.668971937757682	42.17183136055326	17.980582524271846
50	15.913552334086981	22.507248304295786	42.60513366139114	18.974065700226095
51	16.03457906636521	22.56151083920734	41.39832424524538	20.005585849182072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	264.0
2	442.0
3	24320.0
4	48198.0
5	32189.0
6	16180.0
7	15306.0
8	14432.0
9	13742.5
10	13053.0
11	12408.0
12	11763.0
13	10965.0
14	10167.0
15	9265.0
16	8363.0
17	7684.5
18	7006.0
19	6369.0
20	5732.0
21	5137.5
22	4543.0
23	4169.0
24	3795.0
25	3527.0
26	3278.0
27	3297.0
28	3364.0
29	3431.0
30	3567.0
31	3703.0
32	4271.0
33	4839.0
34	5600.0
35	6361.0
36	6586.5
37	6812.0
38	7205.0
39	7598.0
40	8545.5
41	9493.0
42	10653.0
43	11813.0
44	13182.0
45	14551.0
46	16622.5
47	18694.0
48	19852.0
49	21010.0
50	20998.0
51	20986.0
52	18666.5
53	16347.0
54	14695.5
55	13044.0
56	12584.0
57	12124.0
58	12018.0
59	11912.0
60	11979.5
61	12047.0
62	11587.0
63	11127.0
64	9238.0
65	7349.0
66	5966.5
67	4584.0
68	3762.0
69	2940.0
70	2416.0
71	1892.0
72	1572.5
73	1253.0
74	1004.5
75	620.0
76	484.0
77	375.0
78	266.0
79	188.0
80	110.0
81	86.0
82	62.0
83	47.5
84	33.0
85	19.0
86	5.0
87	4.0
88	3.0
89	4.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	375950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.367775195291614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22897164150416	36.39809949581187
2	7.192642734196017	6.52628397250105
3	2.647605192071895	3.603478714794138
4	1.632924789865241	2.963286591097002
5	1.247000379111281	2.8286816433982
6	1.0478711069097975	2.8523748487155043
7	0.9202755477728508	2.922559791835778
8	0.7473529828835567	2.7124593695193453
9	0.6746462629024383	2.7546479992531285
>10	3.5443975692869625	23.738432765873156
>50	0.08383895774824532	2.717689688556903
>100	0.030111174965919092	2.5438496917233304
>500	0.0017712455862305349	0.4580375879590286
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.904151954101782E-4	6.980117838961594
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26059	6.931506849315068	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	605	0.1609256550073148	No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	566	0.15055193509775236	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	539	0.14337012900651683	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	496	0.13193243782417874	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	465	0.12368666046016757	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	462	0.12288868200558585	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	386	0.10267322782284878	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.979784545817263E-4	0.0	0.0	0.08139380236733608	0.0
2	7.979784545817263E-4	0.0	0.0	0.4689453384758612	0.0
3	7.979784545817263E-4	0.0	0.0	0.6772177151216917	0.0
4	7.979784545817263E-4	0.0	0.0	0.903577603404708	0.0
5	7.979784545817263E-4	0.0	0.0	1.6156403777098018	0.0
6	7.979784545817263E-4	0.0	0.0	2.0130336480915014	0.0
7	7.979784545817263E-4	0.0	0.0	2.3803697300172897	0.0
8	7.979784545817263E-4	0.0	0.0	2.938156669769916	0.0
9	7.979784545817263E-4	0.0	0.0	3.143503125415614	0.0
10	7.979784545817263E-4	0.0	0.0	3.6435696236201625	0.0
11	7.979784545817263E-4	0.0	0.0	4.32796914483309	0.0
12	7.979784545817263E-4	0.0	0.0	4.961830030589174	0.0
13	7.979784545817263E-4	0.0	0.0	5.230216784146828	0.0
14	7.979784545817263E-4	0.0	0.0	5.373852905971539	0.0
15	7.979784545817263E-4	0.0	0.0	5.59170102407235	0.0
16	7.979784545817263E-4	0.0	0.0	5.980848517090038	0.0
17	7.979784545817263E-4	0.0	0.0	6.408564968745844	0.0
18	7.979784545817263E-4	0.0	0.0	6.903045617768321	0.0
19	7.979784545817263E-4	0.0	0.0	7.209735337145897	0.0
20	7.979784545817263E-4	0.0	0.0	7.5052533581593295	0.0
21	7.979784545817263E-4	0.0	0.0	7.8768453251762205	0.0
22	7.979784545817263E-4	0.0	0.0	8.262268918739194	0.0
23	7.979784545817263E-4	0.0	0.0	8.626679079664848	0.0
24	7.979784545817263E-4	0.0	0.0	8.936028727224365	0.0
25	7.979784545817263E-4	0.0	0.0	9.21744912887352	0.0
26	7.979784545817263E-4	0.0	0.0	9.465886421066632	0.0
27	7.979784545817263E-4	0.0	0.0	9.720441548078202	0.0
28	7.979784545817263E-4	0.0	0.0	9.991488229817795	0.0
29	7.979784545817263E-4	0.0	0.0	10.24976725628408	0.0
30	7.979784545817263E-4	0.0	0.0	10.54235935629738	0.0
31	0.0010639712727756351	0.0	0.0	10.819789865673627	0.0
32	0.0010639712727756351	0.0	0.0	11.113445936959701	0.0
33	0.0010639712727756351	0.0	0.0	11.402846123154674	0.0
34	0.0010639712727756351	0.0	0.0	11.67602074743982	0.0
35	0.0010639712727756351	0.0	0.0	11.962229019816466	0.0
36	0.0010639712727756351	0.0	0.0	12.257747040829898	0.0
37	0.0010639712727756351	0.0	0.0	12.545019284479318	0.0
38	0.0010639712727756351	0.0	0.0	12.842665248038303	0.0
39	0.0010639712727756351	0.0	0.0	13.15148290996143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	25	3.8857816E-5	45.0	21
AAGTACG	25	3.8857816E-5	45.0	1
ACGGGCC	25	3.8857816E-5	45.0	5
CGATGGT	20	7.0272246E-4	45.0	10
CCACGAG	25	3.8857816E-5	45.0	40
AATACGG	35	1.2090095E-7	45.0	2
CTCGATC	30	2.1615906E-6	44.999996	16
AGTACGG	30	2.1615906E-6	44.999996	2
CGTTTTT	14520	0.0	44.58161	1
TACGAAT	95	0.0	42.63158	12
GTTTTTT	16045	0.0	40.51262	2
GCTACGA	100	0.0	40.5	10
CGCGGGA	75	0.0	39.0	4
CGAATAT	110	0.0	38.863636	14
AGCTACG	105	0.0	38.57143	9
CTAGGGA	105	0.0	38.57143	4
CGTCTTT	35	6.237078E-6	38.571426	1
TCGATCA	35	6.237078E-6	38.571426	17
CACGAGC	30	1.1384613E-4	37.499996	41
GTAGACG	30	1.1384613E-4	37.499996	1
>>END_MODULE