##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935704.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	524029
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36341118525883	33.0	31.0	34.0	30.0	34.0
2	32.57174889175981	34.0	31.0	34.0	31.0	34.0
3	32.432474156964595	34.0	31.0	34.0	30.0	34.0
4	36.03939285802885	37.0	35.0	37.0	35.0	37.0
5	36.06817370794364	37.0	35.0	37.0	35.0	37.0
6	36.07646714208565	37.0	36.0	37.0	35.0	37.0
7	36.236326615511736	37.0	37.0	37.0	35.0	37.0
8	36.03097538495007	37.0	37.0	37.0	35.0	37.0
9	37.836946046879085	39.0	38.0	39.0	35.0	39.0
10	37.659034137423696	39.0	38.0	39.0	35.0	39.0
11	37.6379417932977	39.0	37.0	39.0	35.0	39.0
12	37.5539960574701	39.0	37.0	39.0	35.0	39.0
13	37.54143759219433	39.0	37.0	39.0	35.0	39.0
14	38.8147259025741	41.0	38.0	41.0	35.0	41.0
15	38.95094737123327	41.0	39.0	41.0	35.0	41.0
16	39.03770402019735	41.0	39.0	41.0	35.0	41.0
17	39.01069978951546	41.0	38.0	41.0	35.0	41.0
18	38.38062206480939	39.0	38.0	40.0	35.0	41.0
19	37.69981241496177	37.0	37.0	40.0	35.0	41.0
20	36.90001698379288	36.0	35.0	40.0	34.0	41.0
21	36.86407622478909	36.0	35.0	40.0	34.0	41.0
22	36.86906068175616	36.0	35.0	40.0	34.0	41.0
23	36.874663425115784	36.0	35.0	40.0	34.0	41.0
24	36.71815872785666	36.0	35.0	40.0	34.0	41.0
25	36.62015270147263	35.0	35.0	40.0	33.0	41.0
26	36.47642592299281	35.0	35.0	40.0	33.0	41.0
27	36.39092302143584	35.0	35.0	40.0	33.0	41.0
28	36.26857292249093	35.0	35.0	40.0	33.0	41.0
29	36.04257016310166	35.0	35.0	40.0	33.0	41.0
30	35.529329483673614	35.0	35.0	40.0	31.0	41.0
31	34.890853368802105	35.0	35.0	40.0	25.0	41.0
32	33.90676661024485	35.0	34.0	40.0	18.0	41.0
33	32.78410355152101	35.0	33.0	40.0	12.0	41.0
34	31.809439935576084	35.0	31.0	40.0	8.0	41.0
35	31.21703760669734	35.0	26.0	40.0	7.0	41.0
36	30.848172524803015	35.0	23.0	40.0	7.0	41.0
37	30.676382413950375	35.0	23.0	40.0	7.0	41.0
38	30.499552505681937	35.0	23.0	40.0	7.0	41.0
39	30.355669247312647	35.0	22.0	40.0	7.0	41.0
40	30.21583347486494	35.0	22.0	40.0	7.0	41.0
41	30.03470800280137	35.0	21.0	40.0	7.0	41.0
42	29.955828780468256	35.0	21.0	40.0	7.0	41.0
43	29.874152003038	35.0	21.0	40.0	7.0	41.0
44	29.774002965484733	35.0	20.0	40.0	7.0	41.0
45	29.717775161298324	35.0	20.0	40.0	7.0	41.0
46	29.70284278160178	35.0	20.0	40.0	7.0	41.0
47	29.71631722671837	35.0	20.0	40.0	7.0	41.0
48	29.682191252774178	35.0	20.0	40.0	7.0	41.0
49	29.66644784926025	35.0	20.0	40.0	7.0	41.0
50	29.55395407506073	35.0	20.0	40.0	7.0	41.0
51	28.912821618650877	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	7.0
11	14.0
12	11.0
13	13.0
14	13.0
15	29.0
16	72.0
17	143.0
18	277.0
19	541.0
20	925.0
21	1464.0
22	2367.0
23	4124.0
24	7882.0
25	16128.0
26	26876.0
27	31896.0
28	28843.0
29	22543.0
30	16932.0
31	14285.0
32	14305.0
33	16852.0
34	26771.0
35	34339.0
36	36544.0
37	37341.0
38	66034.0
39	116335.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.878665112045326	13.34449047667209	14.95337090122875	44.82347351005384
2	58.01701814212572	14.851277314805097	15.575855534712774	11.555849008356407
3	20.719463999129818	15.45162576880287	52.57132715937477	11.257583072692542
4	18.63351074081778	16.12677924313349	52.524764850800246	12.714945165248487
5	16.5351535888281	18.78044917361444	51.840069919794516	12.84432731776295
6	16.455959498424704	21.35110843102195	51.98357342818814	10.209358642365213
7	51.245827998068805	3.8536035219424876	41.46163666514639	3.438931814842308
8	51.62214304933506	3.2221499191838623	41.39293817708562	3.762768854395463
9	47.8866627610304	4.47761478849453	42.80755454373708	4.828167906737986
10	26.46800081674869	15.593030156727966	47.23307297878552	10.705896047737816
11	18.669195788782684	16.174868184776034	51.41757421822075	13.738361808220537
12	17.494642472076926	14.469428218667288	54.299094134103264	13.736835175152521
13	16.61778260363453	14.773991515736725	55.75435710619069	12.853868774438057
14	13.527877273967661	16.609386121760437	56.05834791585962	13.804388688412283
15	12.736127199067226	16.379055357623336	57.33613979379004	13.548677649519398
16	15.44609172393131	16.082697713294493	54.40252352446143	14.068687038312767
17	16.06189733774276	15.740159418658129	53.08217674976004	15.11576649383908
18	16.495461129059652	15.219005055063747	54.28783521522664	13.997698600649963
19	15.957704630850584	16.674077198017667	53.02378303490838	14.344435136223376
20	15.86362586803402	17.635474372601518	52.96996921926077	13.530930540103697
21	15.728328012380993	16.849449171706148	54.41263746853705	13.009585347375813
22	15.267857313240299	14.769793274799678	54.53648557617995	15.425863835780081
23	14.308559259124973	16.17391403910852	54.73361207108767	14.783914630678838
24	15.346669745376687	16.121054369128426	54.05769528022304	14.474580605271845
25	15.364989342192894	16.455196181890695	52.88657688792032	15.293237587996083
26	14.257226222212893	16.697740010571934	52.59403582626152	16.45099794095365
27	13.993500359712915	16.02048741577279	54.27848458768503	15.70752763682926
28	12.69910634716781	16.283640790872262	54.04739050701393	16.969862354946006
29	14.310467550459993	16.58381501787115	52.7163573008364	16.389360130832454
30	15.20965442752214	15.751227508401252	52.78467413063017	16.25444393344643
31	15.487310816767774	16.988372780895713	51.34658578055795	16.177730621778565
32	16.553473185644304	18.04690198443216	48.64997929503902	16.749645534884518
33	16.71529629085413	18.652975312435	47.046060427953414	17.585667968757456
34	15.879273856981197	19.939545330506515	46.425865744071416	17.75531506844087
35	16.95974841087039	22.01862874001248	42.16312455990031	18.85849828921682
36	18.19861114556637	22.38444818893611	41.36202385745827	18.054916808039252
37	17.587385430958975	23.312831923424085	41.32214056855632	17.77764207706062
38	18.044612034830134	23.74296079033794	40.17373084314036	18.03869633169157
39	18.288491667445886	22.336168418160064	40.767781935732565	18.607557978661486
40	18.480084117482047	22.385974822004126	40.75881296645797	18.375128094055864
41	16.896965625948184	23.124865226924467	39.12359812147801	20.854571025649342
42	17.352856425884827	23.413208047646215	39.58559545368672	19.64834007278223
43	17.928587921660824	22.73652794024758	39.99396979938133	19.340914338710263
44	17.812372979358013	22.842247280207776	39.68062836217079	19.664751378263418
45	18.033543945087008	21.94477786534715	39.497241564875225	20.52443662469062
46	18.14021743071471	21.78734383020787	40.36436914750901	19.708069591568407
47	16.187462907587175	21.778947348333773	42.484480820717934	19.54910892336111
48	16.289365664877327	21.993630123523698	41.69711981588805	20.019884395710925
49	17.649404899347175	20.858960095719894	42.297277440752325	19.19435756418061
50	16.244902476771323	20.85228107604732	42.742100150945845	20.160716296235513
51	16.140518940745647	20.824420022556005	41.44503453053171	21.590026506166645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	92.0
1	284.5
2	477.0
3	31105.5
4	61734.0
5	40382.5
6	19031.0
7	18253.5
8	17476.0
9	16950.0
10	16424.0
11	16013.0
12	15602.0
13	14460.0
14	13318.0
15	12416.0
16	11514.0
17	10392.0
18	9270.0
19	8427.5
20	7585.0
21	6982.0
22	6379.0
23	5779.0
24	5179.0
25	4925.5
26	4496.0
27	4320.0
28	4684.0
29	5048.0
30	5224.5
31	5401.0
32	6019.5
33	6638.0
34	7495.0
35	8352.0
36	8892.5
37	9433.0
38	10324.0
39	11215.0
40	12859.5
41	14504.0
42	16000.0
43	17496.0
44	19046.5
45	20597.0
46	24207.0
47	27817.0
48	31133.5
49	34450.0
50	34710.0
51	34970.0
52	30938.5
53	26907.0
54	24257.5
55	21608.0
56	20210.0
57	18812.0
58	17571.5
59	16331.0
60	14588.0
61	12845.0
62	11652.0
63	10459.0
64	9534.5
65	8610.0
66	7737.0
67	6864.0
68	6036.5
69	5209.0
70	4172.0
71	3135.0
72	2503.5
73	1872.0
74	1516.0
75	875.0
76	590.0
77	439.5
78	289.0
79	242.5
80	196.0
81	146.5
82	97.0
83	59.0
84	21.0
85	17.5
86	14.0
87	12.0
88	10.0
89	5.0
90	0.0
91	0.0
92	0.0
93	2.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	524029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.551359736667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.39526477547932	36.62113626912451
2	7.673433300905412	6.990706414097252
3	2.8186615736160525	3.8518160194711415
4	1.5461781210168137	2.817220632296029
5	1.0264231940630721	2.337748607741288
6	0.8283251671027694	2.2638802599379835
7	0.6631360573424996	2.1144724371654267
8	0.6341384013685702	2.310869315485974
9	0.5695759501425977	2.335046310206944
>10	3.7373846614469692	25.683495567817726
>50	0.07755332840491008	2.5005085935392324
>100	0.026975070748005633	2.1537703778515582
>500	0.0025289128826255277	0.801952118763234
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.21485480437588E-4	7.217377076501674
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37592	7.173648786612954	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	912	0.174036169753964	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	812	0.15495325640374863	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	693	0.1322445895169924	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	678	0.12938215251446006	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	579	0.1104900682977469	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.63316534008614E-4	0.0	0.0	0.10094861162263921	0.0
2	7.63316534008614E-4	0.0	0.0	0.527070066732948	0.0
3	7.63316534008614E-4	0.0	0.0	0.7749571111522454	0.0
4	7.63316534008614E-4	0.0	0.0	1.0900160105643009	0.0
5	7.63316534008614E-4	0.0	0.0	1.9481746239234852	0.0
6	7.63316534008614E-4	0.0	0.0	2.5031057441477476	0.0
7	9.541456675107676E-4	0.0	0.0	3.0225426455406095	0.0
8	9.541456675107676E-4	0.0	0.0	3.843680407000376	0.0
9	9.541456675107676E-4	0.0	0.0	4.180875485898681	0.0
10	9.541456675107676E-4	0.0	0.0	4.825687128002458	0.0
11	9.541456675107676E-4	0.0	0.0	5.748727646752374	0.0
12	9.541456675107676E-4	0.0	0.0	6.5143341303630145	0.0
13	9.541456675107676E-4	0.0	0.0	6.813554211694391	0.0
14	9.541456675107676E-4	0.0	0.0	6.94904289648092	0.0
15	9.541456675107676E-4	0.0	0.0	7.170213862209916	0.0
16	9.541456675107676E-4	0.0	0.0	7.619998129874491	0.0
17	9.541456675107676E-4	0.0	0.0	8.17187598396272	0.0
18	9.541456675107676E-4	0.0	0.0	8.747416650605215	0.0
19	9.541456675107676E-4	0.0	0.0	9.125067505805976	0.0
20	9.541456675107676E-4	0.0	0.0	9.484398764190532	0.0
21	9.541456675107676E-4	0.0	0.0	9.926931524782026	0.0
22	9.541456675107676E-4	0.0	0.0	10.386638907388713	0.0
23	9.541456675107676E-4	0.0	0.0	10.812378704232017	0.0
24	9.541456675107676E-4	0.0	0.0	11.146138858727284	0.0
25	9.541456675107676E-4	0.0	0.0	11.457953662869803	0.0
26	9.541456675107676E-4	0.0	0.0	11.759654522936708	0.0
27	9.541456675107676E-4	0.0	0.0	12.10810852071164	0.0
28	9.541456675107676E-4	0.0	0.0	12.423739907524203	0.0
29	9.541456675107676E-4	0.0	0.0	12.764560739959048	0.0
30	9.541456675107676E-4	0.0	0.0	13.16263031244454	0.0
31	9.541456675107676E-4	0.0	0.0	13.479406674058115	0.0
32	0.0011449748010129211	0.0	0.0	13.800381276608737	0.0
33	0.0013358039345150745	0.0	0.0	14.128607386232442	0.0
34	0.0013358039345150745	0.0	0.0	14.453207742319604	0.0
35	0.0013358039345150745	0.0	0.0	14.819027191243233	0.0
36	0.0013358039345150745	0.0	0.0	15.130460337118746	0.0
37	0.0013358039345150745	0.0	0.0	15.442847628661772	0.0
38	0.0013358039345150745	0.0	0.0	15.812674489388947	0.0
39	0.0013358039345150745	0.0	0.0	16.379055357623336	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCACGG	20	7.029768E-4	45.000004	2
CCCGGTA	20	7.029768E-4	45.000004	25
CGTTTGG	40	6.8030204E-9	45.000004	2
CCGCACG	20	7.029768E-4	45.000004	38
ATAGCGG	35	1.2102282E-7	45.0	2
ACGGGTA	35	1.2102282E-7	45.0	5
CGTTTTT	18960	0.0	44.584656	1
ACGGGAT	90	0.0	42.5	5
TACGGGA	105	0.0	40.714287	4
ATGGGCG	50	1.0786607E-9	40.5	5
GTTTTTT	20915	0.0	40.48171	2
AACGGGA	85	0.0	39.705883	4
ACACGCG	120	0.0	39.374996	36
ACGGGCC	75	0.0	39.0	5
GCGAGAT	175	0.0	38.571426	18
CGACGGA	35	6.241784E-6	38.571426	29
CGGCCTA	35	6.241784E-6	38.571426	45
TCGGCGT	35	6.241784E-6	38.571426	4
ATGACGG	35	6.241784E-6	38.571426	2
AGTACGG	35	6.241784E-6	38.571426	2
>>END_MODULE