##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935703.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	773665
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38369966329096	33.0	31.0	34.0	30.0	34.0
2	32.5868780415296	34.0	31.0	34.0	31.0	34.0
3	32.44147660809265	34.0	31.0	34.0	30.0	34.0
4	36.061390912087276	37.0	35.0	37.0	35.0	37.0
5	36.09482140202801	37.0	35.0	37.0	35.0	37.0
6	36.109030394292105	37.0	36.0	37.0	35.0	37.0
7	36.25208714366037	37.0	37.0	37.0	35.0	37.0
8	36.0313507784377	37.0	37.0	37.0	35.0	37.0
9	37.86100185480796	39.0	38.0	39.0	35.0	39.0
10	37.628340431582146	39.0	38.0	39.0	35.0	39.0
11	37.62226932845611	39.0	37.0	39.0	35.0	39.0
12	37.556055915674094	39.0	37.0	39.0	35.0	39.0
13	37.55468064343094	39.0	37.0	39.0	35.0	39.0
14	38.9042893241907	41.0	38.0	41.0	35.0	41.0
15	39.01360924948136	41.0	39.0	41.0	35.0	41.0
16	39.09803597164147	41.0	39.0	41.0	35.0	41.0
17	39.0614451991495	41.0	39.0	41.0	35.0	41.0
18	38.40577898702927	39.0	38.0	40.0	35.0	41.0
19	37.71124453090162	37.0	37.0	40.0	35.0	41.0
20	36.88504197553205	36.0	35.0	40.0	34.0	41.0
21	36.870540867171194	36.0	35.0	40.0	34.0	41.0
22	36.87404109013591	36.0	35.0	40.0	34.0	41.0
23	36.873411618723864	36.0	35.0	40.0	34.0	41.0
24	36.722483245332285	36.0	35.0	40.0	34.0	41.0
25	36.65107637026362	35.0	35.0	40.0	33.0	41.0
26	36.49607000445929	35.0	35.0	40.0	33.0	41.0
27	36.40271823075879	35.0	35.0	40.0	33.0	41.0
28	36.26261754118384	35.0	35.0	40.0	33.0	41.0
29	36.01077468930351	35.0	35.0	40.0	33.0	41.0
30	35.472244446885924	35.0	35.0	40.0	31.0	41.0
31	34.78846141417797	35.0	35.0	40.0	23.0	41.0
32	33.687261282337964	35.0	33.0	40.0	17.0	41.0
33	32.47390925012764	35.0	32.0	40.0	12.0	41.0
34	31.43440377941357	35.0	27.0	40.0	7.0	41.0
35	30.82400651444747	35.0	23.0	40.0	7.0	41.0
36	30.470220314994215	35.0	22.0	40.0	7.0	41.0
37	30.30609501528439	35.0	21.0	40.0	7.0	41.0
38	30.174613043112974	35.0	21.0	40.0	7.0	41.0
39	30.042249552454873	35.0	20.0	40.0	7.0	41.0
40	29.932276889868355	35.0	20.0	40.0	7.0	41.0
41	29.76254709725786	35.0	18.0	40.0	7.0	41.0
42	29.676124679286254	35.0	18.0	40.0	7.0	41.0
43	29.58618265011342	35.0	18.0	40.0	7.0	41.0
44	29.480978201159417	35.0	18.0	40.0	7.0	41.0
45	29.420574796585086	35.0	18.0	40.0	7.0	41.0
46	29.371698344890877	35.0	18.0	40.0	7.0	41.0
47	29.348086057919126	35.0	18.0	40.0	7.0	41.0
48	29.29040476175089	35.0	18.0	40.0	7.0	41.0
49	29.272170771587184	35.0	18.0	40.0	7.0	41.0
50	29.157148119664196	35.0	18.0	40.0	7.0	41.0
51	28.52767670761893	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	15.0
10	16.0
11	16.0
12	15.0
13	18.0
14	24.0
15	51.0
16	70.0
17	193.0
18	422.0
19	794.0
20	1412.0
21	2216.0
22	3924.0
23	6618.0
24	13122.0
25	27184.0
26	43745.0
27	49827.0
28	43508.0
29	32894.0
30	25135.0
31	21147.0
32	20210.0
33	22803.0
34	36431.0
35	47962.0
36	46811.0
37	49323.0
38	93819.0
39	183775.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.30160986990493	13.225103888633969	14.879825247361584	44.593460994099516
2	58.257256047514105	15.599516586636335	14.944323447486962	11.198903918362598
3	19.89750085631378	16.209211997440754	52.63453820452004	11.258748941725424
4	17.397969405362787	16.151952072279347	53.889732636218525	12.560345886139348
5	16.72687791227469	18.560100301810216	52.41092721009739	12.302094575817698
6	16.359406202943134	22.187768607859994	52.03401989233066	9.418805296866214
7	50.46383124478942	3.7940193753110196	42.4986266665805	3.243522713319072
8	51.07378516541397	3.8665313798607923	41.68432073313385	3.3753627215913866
9	47.53762933569439	4.484499104909748	42.95153587146891	5.026335687926945
10	25.727026555421272	16.06470500798149	48.25680365532886	9.951464781268378
11	19.559887031208596	16.353331222169803	51.820490780893536	12.26629096572806
12	17.549456159965874	14.72200500216502	54.572586326123066	13.155952511746042
13	16.35462377127051	15.085857574014593	56.26362831458061	12.295890340134296
14	13.580554891328934	17.72847421041407	55.48861587379551	13.202355024461493
15	13.025405052574435	16.5896091977794	57.52825835471425	12.856727394931916
16	15.326013196926318	16.582758687545642	54.933853799771214	13.15737431575682
17	15.68818545494497	16.341827535173493	54.00722534947297	13.962761660408574
18	16.13540744379027	16.01261527922292	54.80020422275791	13.051773054228896
19	15.428383085702468	17.466862272430575	53.88029702778334	13.224457614083615
20	15.828685542192034	17.791292096708524	54.25823838483065	12.1217839762688
21	15.32911531476802	17.918737438038427	54.891587444178036	11.860559803015517
22	14.553456599432572	15.41571610451552	55.42372990893991	14.607097387111992
23	13.907052794168017	17.096029935437173	55.17711154052465	13.819805729870163
24	14.279306935172201	16.443551149399287	55.059101807629915	14.218040107798597
25	14.443460670962239	17.625845811817776	53.3369093858453	14.59378413137469
26	13.484259983326115	17.240278415076293	54.08503680533564	15.190424796261947
27	14.028423154724589	16.839846703676656	54.80847653700245	14.323253604596303
28	12.471418508010574	17.459882507286746	54.543891736087325	15.524807248615357
29	13.74419160747869	17.28487135905075	53.32980036579139	15.641136667679165
30	14.574783659594267	17.246224140939553	53.293996755701755	14.88499544376442
31	14.570906012292143	18.634938894741264	50.657196590255474	16.13695850271112
32	15.315802059030718	20.472426696309125	48.68993685897643	15.521834385683725
33	15.93493307827031	21.179968074037212	45.97222311982577	16.912875727866712
34	15.34346260978589	22.750673741218744	44.1356401026284	17.770223546366967
35	16.24928101956273	24.701518098918783	41.419218912578444	17.629981968940044
36	16.926447493424156	25.823321463424094	39.50973612610109	17.74049491705066
37	16.652039319343643	26.517678840324947	39.79642351663834	17.03385832369307
38	17.553333807268004	26.114015756173536	38.06518325114875	18.267467185409707
39	17.713351385935773	25.80264067781275	38.011671718379404	18.472336217872076
40	18.922014050008727	24.309875721403966	38.87380196855228	17.89430826003503
41	17.3841391299852	25.48144222628657	37.991895717138554	19.142522926589674
42	17.977677677030755	24.744947748702604	38.914905029954824	18.362469544311814
43	17.732222602806125	24.601991818164194	38.82248776925413	18.84329780977555
44	17.23006727718069	24.338182546709493	38.281297460787286	20.150452715322523
45	17.338253636910032	23.781869413764355	38.19030200409738	20.68957494522823
46	17.88590669088042	24.12155131743067	38.55945402725986	19.43308796442905
47	15.981594100805903	24.36855745057615	40.78819644161232	18.861652007005617
48	16.452081973463965	24.189539400127963	40.02727278602496	19.33110584038311
49	17.03631416698442	23.096430625658392	40.799700128608634	19.067555078748555
50	16.33433075038938	22.8348186876749	40.57699391855648	20.25385664337924
51	15.95225323621981	22.869200493753755	39.16553030058229	22.01301596944414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	370.0
2	594.0
3	42925.5
4	85257.0
5	56626.0
6	27995.0
7	27036.0
8	26077.0
9	25747.5
10	25418.0
11	24333.5
12	23249.0
13	22141.0
14	21033.0
15	19529.0
16	18025.0
17	16441.5
18	14858.0
19	13758.0
20	12658.0
21	11550.0
22	10442.0
23	9805.0
24	9168.0
25	8621.5
26	7856.5
27	7638.0
28	8088.5
29	8539.0
30	8825.5
31	9112.0
32	10188.5
33	11265.0
34	12741.0
35	14217.0
36	15562.5
37	16908.0
38	18451.0
39	19994.0
40	21135.0
41	22276.0
42	24744.5
43	27213.0
44	31309.5
45	35406.0
46	41591.0
47	47776.0
48	49103.0
49	50430.0
50	50208.5
51	49987.0
52	44709.5
53	39432.0
54	35306.5
55	31181.0
56	28316.5
57	25452.0
58	23001.5
59	20551.0
60	18726.5
61	16902.0
62	14826.5
63	12751.0
64	10662.5
65	8574.0
66	7172.5
67	5771.0
68	4883.0
69	3995.0
70	3101.5
71	2208.0
72	1733.0
73	1258.0
74	1102.0
75	694.5
76	443.0
77	310.5
78	178.0
79	164.0
80	150.0
81	106.5
82	63.0
83	47.0
84	31.0
85	23.0
86	15.0
87	9.0
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	773665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.48858754479546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75942080062461	35.92872562355563
2	7.863410053372963	6.996640131202156
3	2.8182541585078944	3.761404405627869
4	1.4556624785653836	2.5904147045332007
5	0.9755220521549374	2.1699799109586726
6	0.7361772433603595	1.9650891443834126
7	0.5799612488562361	1.8061159754630696
8	0.5082657207631325	1.8089619211351347
9	0.44110591583428665	1.7661761237808757
>10	3.7473650571350485	27.879436241670764
>50	0.06416660811283051	2.0003626011551474
>100	0.04629369195703555	3.3541840615929117
>500	0.0026369824532447993	0.7951392342490572
>1k	0.0014649902518026662	0.8561433591061981
>5k	0.0	0.0
>10k+	2.929980503605333E-4	6.321226561585866
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48494	6.268087608978046	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	1588	0.20525679719258336	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	1452	0.18767812942294146	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	1245	0.1609223630382659	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1173	0.1516160095131614	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1110	0.1434729501786949	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	926	0.11969004672564998	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	823	0.10637679098834767	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.170196402835852E-4	0.0	0.0	0.0950023589021088	0.0
2	5.170196402835852E-4	0.0	0.0	0.4361060665792042	0.0
3	5.170196402835852E-4	0.0	0.0	0.6478256092753324	0.0
4	5.170196402835852E-4	0.0	0.0	0.9519624126721514	0.0
5	5.170196402835852E-4	0.0	0.0	1.8290862324132537	0.0
6	5.170196402835852E-4	0.0	0.0	2.308880458596421	0.0
7	5.170196402835852E-4	0.0	0.0	2.7645040165963306	0.0
8	5.170196402835852E-4	0.0	0.0	3.511597396806111	0.0
9	5.170196402835852E-4	0.0	0.0	3.7766992173615197	0.0
10	5.170196402835852E-4	0.0	0.0	4.530126088164774	0.0
11	5.170196402835852E-4	0.0	0.0	5.393936652168574	0.0
12	5.170196402835852E-4	0.0	0.0	6.306605572179173	0.0
13	5.170196402835852E-4	0.0	0.0	6.598721668939399	0.0
14	5.170196402835852E-4	0.0	0.0	6.734180814693699	0.0
15	5.170196402835852E-4	0.0	0.0	6.969166241202588	0.0
16	5.170196402835852E-4	0.0	0.0	7.411605798375266	0.0
17	5.170196402835852E-4	0.0	0.0	7.939224341284665	0.0
18	5.170196402835852E-4	0.0	0.0	8.52125920133391	0.0
19	5.170196402835852E-4	0.0	0.0	8.923888246204752	0.0
20	5.170196402835852E-4	0.0	0.0	9.311265211687228	0.0
21	5.170196402835852E-4	0.0	0.0	9.784208927636639	0.0
22	6.462745503544816E-4	0.0	0.0	10.278350448837676	0.0
23	6.462745503544816E-4	0.0	0.0	10.758532439751056	0.0
24	6.462745503544816E-4	0.0	0.0	11.135698267337931	0.0
25	6.462745503544816E-4	0.0	0.0	11.471373268792048	0.0
26	6.462745503544816E-4	0.0	0.0	11.795156818519644	0.0
27	7.755294604253779E-4	0.0	0.0	12.14233550697007	0.0
28	7.755294604253779E-4	0.0	0.0	12.464826507596957	0.0
29	7.755294604253779E-4	0.0	0.0	12.82557696160483	0.0
30	7.755294604253779E-4	0.0	0.0	13.234539497069145	0.0
31	9.047843704962742E-4	0.0	0.0	13.595936225627371	0.0
32	9.047843704962742E-4	0.0	0.0	13.932516011451986	0.0
33	0.0010340392805671705	0.0	0.0	14.279177680262128	0.0
34	0.0010340392805671705	0.0	0.0	14.626614878532699	0.0
35	0.0010340392805671705	0.0	0.0	14.991242979842697	0.0
36	0.0010340392805671705	0.0	0.0	15.322652569264475	0.0
37	0.0010340392805671705	0.0	0.0	15.677586552319156	0.0
38	0.0010340392805671705	0.0	0.0	16.05875928211823	0.0
39	0.0010340392805671705	0.0	0.0	16.575908177311884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATGCG	70	0.0	45.000004	1
TACTTCG	25	3.88962E-5	45.0	28
TTAGACG	20	7.0318556E-4	45.0	1
CGATTCG	25	3.88962E-5	45.0	10
ACGATAG	20	7.0318556E-4	45.0	1
CGTAGCG	20	7.0318556E-4	45.0	1
ACTACGG	20	7.0318556E-4	45.0	2
GCGATTC	40	6.8102963E-9	45.0	9
CACCGGT	20	7.0318556E-4	45.0	40
TACGTAC	20	7.0318556E-4	45.0	13
TAATCGG	20	7.0318556E-4	45.0	2
ACGCACT	30	2.1645792E-6	44.999996	40
TACGCAC	30	2.1645792E-6	44.999996	39
GCTAGCG	60	0.0	44.999996	1
CGTTTTT	28735	0.0	44.639812	1
CGTAAGG	110	0.0	40.909092	2
GTTTTTT	32145	0.0	40.04433	2
GGCGTAG	45	1.9268555E-8	40.0	1
TACGAGG	45	1.9268555E-8	40.0	2
TCGTAAG	45	1.9268555E-8	40.0	1
>>END_MODULE