##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935701.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535001
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47037669088469	34.0	31.0	34.0	31.0	34.0
2	32.668346414305766	34.0	31.0	34.0	31.0	34.0
3	32.51567193332349	34.0	31.0	34.0	31.0	34.0
4	36.110962409416054	37.0	35.0	37.0	35.0	37.0
5	36.14460720634167	37.0	35.0	37.0	35.0	37.0
6	36.16788940581419	37.0	37.0	37.0	35.0	37.0
7	36.30345737671519	37.0	37.0	37.0	35.0	37.0
8	36.10966895388981	37.0	37.0	37.0	35.0	37.0
9	37.90725624811916	39.0	38.0	39.0	35.0	39.0
10	37.74765467728098	39.0	38.0	39.0	35.0	39.0
11	37.735361242315435	39.0	38.0	39.0	35.0	39.0
12	37.700514578477424	39.0	38.0	39.0	35.0	39.0
13	37.72378556301764	39.0	38.0	39.0	35.0	39.0
14	39.170850147943646	41.0	39.0	41.0	35.0	41.0
15	39.25227616396979	41.0	39.0	41.0	35.0	41.0
16	39.295889166562304	41.0	39.0	41.0	35.0	41.0
17	39.26112100725045	41.0	39.0	41.0	35.0	41.0
18	38.60517830807793	39.0	39.0	41.0	35.0	41.0
19	37.92728985553298	37.0	37.0	41.0	35.0	41.0
20	37.12243154685692	37.0	35.0	41.0	34.0	41.0
21	37.09163347358229	37.0	35.0	41.0	34.0	41.0
22	37.074558739142546	37.0	35.0	41.0	34.0	41.0
23	37.07341855435784	37.0	35.0	41.0	34.0	41.0
24	36.92574780234056	37.0	35.0	41.0	34.0	41.0
25	36.8414059039142	36.0	35.0	41.0	34.0	41.0
26	36.69341178801535	36.0	35.0	40.0	33.0	41.0
27	36.61867921742202	36.0	35.0	40.0	33.0	41.0
28	36.472969209403345	36.0	35.0	40.0	33.0	41.0
29	36.209285590120395	36.0	35.0	40.0	33.0	41.0
30	35.69622673602479	36.0	35.0	40.0	31.0	41.0
31	35.01575511073811	36.0	35.0	40.0	24.0	41.0
32	33.962431845921785	36.0	34.0	40.0	18.0	41.0
33	32.75149392244127	36.0	33.0	40.0	12.0	41.0
34	31.761699510842035	36.0	30.0	40.0	8.0	41.0
35	31.151459529982187	36.0	24.0	40.0	7.0	41.0
36	30.7721387436659	36.0	23.0	40.0	7.0	41.0
37	30.584180216485578	35.0	22.0	40.0	7.0	41.0
38	30.39934878626395	35.0	21.0	40.0	7.0	41.0
39	30.23869488094415	35.0	21.0	40.0	7.0	41.0
40	30.096098885796476	35.0	20.0	40.0	7.0	41.0
41	29.925463690722072	35.0	19.0	40.0	7.0	41.0
42	29.80552372799303	35.0	18.0	40.0	7.0	41.0
43	29.693008050452242	35.0	18.0	40.0	7.0	41.0
44	29.575868082489567	35.0	18.0	40.0	7.0	41.0
45	29.493754217281836	35.0	18.0	40.0	7.0	41.0
46	29.429202936069277	35.0	18.0	40.0	7.0	41.0
47	29.427288920955288	35.0	18.0	40.0	7.0	41.0
48	29.382027323313416	35.0	18.0	40.0	7.0	41.0
49	29.356451670183795	35.0	18.0	40.0	7.0	41.0
50	29.254715411746893	35.0	18.0	40.0	7.0	41.0
51	28.67757069612954	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	7.0
10	7.0
11	8.0
12	12.0
13	6.0
14	20.0
15	25.0
16	45.0
17	101.0
18	260.0
19	519.0
20	856.0
21	1385.0
22	2400.0
23	4169.0
24	8662.0
25	17753.0
26	29853.0
27	35295.0
28	30296.0
29	22489.0
30	16822.0
31	13980.0
32	12838.0
33	14140.0
34	21380.0
35	30934.0
36	32840.0
37	34761.0
38	66160.0
39	136835.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.363317077912008	13.786703202423919	15.860531101811024	44.98944861785305
2	57.78437797312529	15.492494406552511	15.357728303311582	11.365399317010622
3	20.50781213493059	15.937540303662983	52.37934134702552	11.175306214380907
4	18.456414100160558	16.40258616339035	52.57560266242493	12.56539707402416
5	16.37903480554242	19.442767396696457	51.94756645314682	12.23063134461431
6	17.030996203745413	22.005005598120377	51.0442036556941	9.919794542440108
7	51.941958986992546	3.749899532898069	41.19151179156674	3.116629688542638
8	52.7913031938258	3.452143080106392	40.757306995687856	2.999246730379943
9	48.41224595841877	5.617559593346554	42.13543526086867	3.8347591873660045
10	23.53715226700511	21.41902538499928	46.20178280040598	8.84203954758963
11	18.185573484909373	17.14482776667707	52.682705265971464	11.986893482442088
12	17.16127633406293	15.369503982235546	54.7632621247437	12.705957558957834
13	15.598662432406668	16.375296494772908	55.08139237122921	12.944648701591213
14	13.02390088990488	18.399404860925493	54.229805177934246	14.346889071235381
15	12.364649785701335	18.165012775677056	56.63484741150017	12.835490027121443
16	14.587075538176563	17.27361257268678	55.2417659032413	12.897545985895354
17	14.410066523240145	16.933426292661135	53.609619421272114	15.04688776282661
18	14.939411328203125	17.293051788688246	54.385692737022914	13.381844146085708
19	15.488756095783	17.76763034087787	53.14943336554511	13.594180197794023
20	16.446885146009073	17.230061252221958	53.53018031741996	12.792873284349001
21	15.745391130110036	17.623144629636204	53.78345087205445	12.848013368199313
22	15.25567241930389	16.350997474771077	53.44662907172136	14.946701034203674
23	14.472309397552527	17.526135465167357	53.892235715447256	14.109319421832856
24	14.390253476161726	16.928566488660767	54.11952501023363	14.561655024943878
25	14.224272477995367	18.176975370139495	53.343264778944345	14.255487372920797
26	13.938291704127655	18.225760325681634	53.16532118631554	14.670626783875171
27	14.182777228453777	17.63099508225218	53.60120822204071	14.585019467253332
28	12.900162803434013	18.162022127061444	54.014665393148796	14.923149676355745
29	13.614553991487865	17.34800495700008	53.84756290175159	15.18987814976047
30	14.21642202537939	18.07398490843942	52.05167840807774	15.657914658103442
31	14.845579727888358	19.231365922680517	50.12214930439383	15.8009050450373
32	15.44034497131781	21.03042798050845	48.224395842250765	15.30483120592298
33	15.15342961975772	22.432855265691092	45.75524157898771	16.658473535563484
34	15.419597346547015	23.5793951787006	43.766273334068536	17.234734140683848
35	16.560529793402257	23.890609550262525	41.3859039515814	18.16295670475382
36	16.690062261565867	25.88350302148968	39.49487944882346	17.931555268120995
37	17.36987407500173	26.034717692116466	39.157123070798	17.438285162083808
38	17.493238330395645	26.581071811080726	37.962919695477204	17.962770163046425
39	18.166134268907907	25.703690273476127	38.01189156655782	18.118283891058148
40	18.871553511114932	25.32593397021688	38.82048818600339	16.9820243326648
41	17.54987373855376	25.396214212683716	38.039368150713734	19.01454389804879
42	18.14594739075254	25.257335967596322	37.441986089745626	19.154730551905512
43	18.166134268907907	25.021448558040078	37.523481264521	19.288935908531013
44	17.672864162870724	25.421634725916398	37.17656602511023	19.72893508610264
45	17.736976192567862	24.919018842955435	37.331332090968054	20.012672873508645
46	18.02875134812832	25.11079418543143	37.62871471268278	19.23173975375747
47	17.279406954379525	24.2919172113697	39.36142175435186	19.067254079898916
48	17.13211751006073	23.44780663961376	39.75431821622764	19.66575763409788
49	17.346509632692275	23.226498642058612	39.61543997114024	19.811551754108873
50	16.32482929938449	23.415470251457474	39.949831869473144	20.3098685796849
51	16.439782355547	23.26425558083069	38.617497911218855	21.678464152403453
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	260.5
2	419.0
3	27733.0
4	55047.0
5	37550.0
6	20053.0
7	19341.0
8	18629.0
9	17952.0
10	17275.0
11	16877.0
12	16479.0
13	15572.0
14	14665.0
15	13762.5
16	12860.0
17	11864.0
18	10868.0
19	9961.0
20	9054.0
21	8221.0
22	7388.0
23	6820.5
24	6253.0
25	5884.0
26	5449.5
27	5384.0
28	5788.0
29	6192.0
30	6848.5
31	7505.0
32	8009.0
33	8513.0
34	9529.0
35	10545.0
36	11574.5
37	12604.0
38	13298.0
39	13992.0
40	15609.0
41	17226.0
42	18845.5
43	20465.0
44	22571.0
45	24677.0
46	27415.5
47	30154.0
48	31893.5
49	33633.0
50	32962.0
51	32291.0
52	28632.0
53	24973.0
54	22521.5
55	20070.0
56	18088.5
57	16107.0
58	15080.5
59	14054.0
60	13226.0
61	12398.0
62	11044.0
63	9690.0
64	8565.0
65	7440.0
66	5978.0
67	4516.0
68	3825.5
69	3135.0
70	2560.0
71	1985.0
72	1632.5
73	1280.0
74	1061.0
75	613.5
76	385.0
77	276.5
78	168.0
79	146.5
80	125.0
81	76.0
82	27.0
83	18.5
84	10.0
85	7.0
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	535001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.23229096171148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51609543255236	36.419274557061364
2	7.279988897941199	6.585811520594112
3	2.7167067708760246	3.686486133537479
4	1.6012358092682806	2.897102560925376
5	1.157989056259804	2.6189248961610567
6	0.8923616312957214	2.421813656990134
7	0.7351136817955951	2.327561316143934
8	0.6776883409423913	2.4522716975092615
9	0.5615182368023507	2.285888064061602
>10	3.7459079393529535	26.590744022971492
>50	0.08384758629207575	2.6022619452640594
>100	0.028225920134818384	2.196339122158409
>500	0.002905609425643069	0.8351270657685592
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.150870608061527E-4	6.080393440853145
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32385	6.0532597135332455	No Hit
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	733	0.1370090897026361	RNA PCR Primer, Index 13 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	720	0.1345791877024529	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	673	0.12579415739409833	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	622	0.11626146493184125	No Hit
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	606	0.11327081631623119	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	553	0.10336429277702285	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	541	0.1011213063153153	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09476617800714392	0.0
2	0.0	0.0	0.0	0.43513937357126437	0.0
3	0.0	0.0	0.0	0.6622417528191537	0.0
4	0.0	0.0	0.0	0.9246711688389367	0.0
5	0.0	0.0	0.0	1.6295296644305337	0.0
6	0.0	0.0	0.0	2.0820521830800316	0.0
7	0.0	0.0	0.0	2.471210334186291	0.0
8	0.0	0.0	0.0	3.0947605705409895	0.0
9	0.0	0.0	0.0	3.336068530713027	0.0
10	0.0	0.0	0.0	3.8895254401393644	0.0
11	0.0	0.0	0.0	4.589337216192119	0.0
12	0.0	0.0	0.0	5.259616337165725	0.0
13	0.0	0.0	0.0	5.543914871187157	0.0
14	0.0	0.0	0.0	5.656438025349486	0.0
15	0.0	0.0	0.0	5.854194665056701	0.0
16	0.0	0.0	0.0	6.219614542776555	0.0
17	0.0	0.0	0.0	6.689894037581238	0.0
18	0.0	0.0	0.0	7.171762295771409	0.0
19	0.0	0.0	0.0	7.488957964564552	0.0
20	0.0	0.0	0.0	7.800546167203426	0.0
21	0.0	0.0	0.0	8.184096852155417	0.0
22	0.0	0.0	0.0	8.61026427987985	0.0
23	0.0	0.0	0.0	9.03231956575782	0.0
24	1.8691553847562903E-4	0.0	0.0	9.384281524707431	0.0
25	1.8691553847562903E-4	0.0	0.0	9.683159470729962	0.0
26	1.8691553847562903E-4	0.0	0.0	9.970448653367002	0.0
27	1.8691553847562903E-4	0.0	0.0	10.278111629697888	0.0
28	1.8691553847562903E-4	0.0	0.0	10.570634447412248	0.0
29	1.8691553847562903E-4	0.0	0.0	10.86390492728051	0.0
30	1.8691553847562903E-4	0.0	0.0	11.227642565154083	0.0
31	1.8691553847562903E-4	0.0	0.0	11.567081183025826	0.0
32	3.7383107695125806E-4	0.0	0.0	11.886893669357628	0.0
33	3.7383107695125806E-4	0.0	0.0	12.188762263995768	0.0
34	3.7383107695125806E-4	0.0	0.0	12.493434591711043	0.0
35	3.7383107695125806E-4	0.0	0.0	12.827639574505469	0.0
36	3.7383107695125806E-4	0.0	0.0	13.165209036992454	0.0
37	3.7383107695125806E-4	0.0	0.0	13.486329932093584	0.0
38	3.7383107695125806E-4	0.0	0.0	13.812123715656606	0.0
39	3.7383107695125806E-4	0.0	0.0	14.133244610757737	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	20	7.029902E-4	45.0	1
GACGTAG	20	7.029902E-4	45.0	1
TAACGTG	20	7.029902E-4	45.0	1
ACGGGTC	40	6.8030204E-9	45.0	5
TAGTTCG	20	7.029902E-4	45.0	1
AACGGGC	35	1.210301E-7	45.0	4
AAATGCG	20	7.029902E-4	45.0	1
CGTTTTT	19580	0.0	44.52886	1
TACGAAT	85	0.0	42.352943	12
CGAATAT	85	0.0	42.352943	14
CGTGCGG	70	0.0	41.785713	2
CGACCAG	45	1.9250365E-8	40.0	12
GCTACGA	90	0.0	40.0	10
CCTATGC	90	0.0	40.0	35
AGCTACG	90	0.0	40.0	9
GTTTTTT	21890	0.0	39.963455	2
ACGGGAG	170	0.0	39.705883	5
TACGGGT	40	3.453606E-7	39.375	4
CCTCGTC	75	0.0	39.0	36
CGAACGG	35	6.2420295E-6	38.571426	2
>>END_MODULE