##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935697.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398435
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38385182024672	33.0	31.0	34.0	30.0	34.0
2	32.58330969919811	34.0	31.0	34.0	31.0	34.0
3	32.43325009098096	34.0	31.0	34.0	30.0	34.0
4	36.04973960620929	37.0	35.0	37.0	35.0	37.0
5	36.094908830800506	37.0	35.0	37.0	35.0	37.0
6	36.112296861470504	37.0	36.0	37.0	35.0	37.0
7	36.25057035651989	37.0	37.0	37.0	35.0	37.0
8	36.000559689786286	37.0	37.0	37.0	35.0	37.0
9	37.82301253654925	39.0	38.0	39.0	35.0	39.0
10	37.68891287161018	39.0	38.0	39.0	35.0	39.0
11	37.655429367399954	39.0	37.0	39.0	35.0	39.0
12	37.61819870242323	39.0	37.0	39.0	35.0	39.0
13	37.59786665328096	39.0	37.0	39.0	35.0	39.0
14	39.01636653406453	41.0	39.0	41.0	35.0	41.0
15	39.12100593572352	41.0	39.0	41.0	35.0	41.0
16	39.15758906722552	41.0	39.0	41.0	35.0	41.0
17	39.11392824425565	41.0	39.0	41.0	35.0	41.0
18	38.47121864294051	39.0	38.0	41.0	35.0	41.0
19	37.778455707957384	37.0	37.0	41.0	35.0	41.0
20	36.979090692333756	36.0	35.0	40.0	34.0	41.0
21	36.95369131727885	36.0	35.0	40.0	34.0	41.0
22	36.93628069823183	36.0	35.0	40.0	34.0	41.0
23	36.95421837940944	36.0	35.0	40.0	34.0	41.0
24	36.79153437825492	36.0	35.0	40.0	34.0	41.0
25	36.71005810232535	36.0	35.0	40.0	34.0	41.0
26	36.552117660346106	35.0	35.0	40.0	33.0	41.0
27	36.48661889643229	35.0	35.0	40.0	33.0	41.0
28	36.343177682683496	35.0	35.0	40.0	33.0	41.0
29	36.11775822907124	35.0	35.0	40.0	33.0	41.0
30	35.59384843199016	35.0	35.0	40.0	31.0	41.0
31	34.93573104772422	35.0	35.0	40.0	25.0	41.0
32	33.91671163426908	35.0	34.0	40.0	18.0	41.0
33	32.7954145594639	35.0	33.0	40.0	12.0	41.0
34	31.86888702046758	35.0	31.0	40.0	8.0	41.0
35	31.304792500658827	35.0	26.0	40.0	7.0	41.0
36	30.9616499554507	35.0	23.0	40.0	7.0	41.0
37	30.79976407695107	35.0	23.0	40.0	7.0	41.0
38	30.66195238872087	35.0	23.0	40.0	7.0	41.0
39	30.54598115125428	35.0	23.0	40.0	7.0	41.0
40	30.40910060612145	35.0	22.0	40.0	7.0	41.0
41	30.219707104044573	35.0	22.0	40.0	7.0	41.0
42	30.10796491272102	35.0	21.0	40.0	7.0	41.0
43	29.9937630981214	35.0	21.0	40.0	7.0	41.0
44	29.887657961775446	35.0	20.0	40.0	7.0	41.0
45	29.835862813256867	35.0	20.0	40.0	7.0	41.0
46	29.783490406214312	35.0	20.0	40.0	7.0	41.0
47	29.805792663797106	35.0	20.0	40.0	7.0	41.0
48	29.753249589017027	35.0	20.0	40.0	7.0	41.0
49	29.74484169312435	35.0	20.0	40.0	7.0	41.0
50	29.633069383964763	35.0	20.0	40.0	7.0	41.0
51	29.047335198965953	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	9.0
11	6.0
12	6.0
13	8.0
14	13.0
15	16.0
16	47.0
17	89.0
18	237.0
19	386.0
20	659.0
21	977.0
22	1539.0
23	2812.0
24	5727.0
25	11726.0
26	20062.0
27	25028.0
28	22484.0
29	17315.0
30	12777.0
31	11187.0
32	10396.0
33	11484.0
34	18264.0
35	27715.0
36	28069.0
37	25837.0
38	48493.0
39	94964.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.716528417433206	12.935359594413143	14.876203144804045	45.47190884334961
2	58.47478258687113	15.41807321143976	14.961537013565577	11.145607188123535
3	20.776036241796028	15.201726755932587	53.073148694266315	10.94908830800507
4	18.398735050886593	16.064602758291816	53.058340758216524	12.478321432605068
5	16.409200998908226	19.310050572866338	51.438754125516084	12.84199430270935
6	16.783666093591176	21.962177017581286	51.49020542873994	9.763951460087593
7	50.084204449910274	4.238081493844667	42.05253052568173	3.625183530563329
8	50.0217099401408	4.207963657811186	41.92578463237416	3.844541769673849
9	46.1104069672594	5.453085195828679	43.60334809943905	4.8331597374728625
10	25.215907237065018	17.60513007140437	47.89388482437537	9.285077867155245
11	19.001593735490104	16.556778395472286	51.962553490531704	12.479074378505905
12	18.331471883745152	14.847089236638347	54.368215643705994	12.4532232359105
13	15.189428639552249	16.173277949979294	55.475548081870315	13.16174532859814
14	12.596784921003426	18.594250003137276	54.64329187947846	14.16567319638084
15	12.227088483692446	17.235182652126444	57.515278527237825	13.022450336943288
16	13.611505013364788	16.719665692020026	56.35398496618018	13.314844328435003
17	13.119580358151268	16.746269780516272	54.176967384893395	15.957182476439069
18	14.306474079837365	16.903133509857316	55.01298831678943	13.77740409351588
19	15.244644672280296	17.577020091106455	53.378342766072265	13.799992470540992
20	16.364777190758844	17.03414609660296	53.91142846386487	12.689648248773326
21	15.431375255687879	17.319512593020193	54.136559288215146	13.112552863076788
22	14.056747022726418	16.345702561270972	53.61501876090203	15.982531655100582
23	13.717921367349756	17.013063611379522	54.33006638473026	14.938948636540465
24	14.565738451692244	15.921041073198891	54.56247568612195	14.950744788986912
25	13.682532910010417	16.988467378618846	53.50308080364425	15.82591890772648
26	12.538808086638975	18.091784105312033	54.05473916699085	15.314668641058141
27	14.367211715838216	17.24296309310176	54.455306386236145	13.934518804823872
28	11.573782423732855	18.191674928156413	55.260958500131764	14.973584147978968
29	13.664462208390326	16.211678190921983	54.3973295518717	15.726530048815993
30	14.136559288215143	16.645876993738	54.12275528003313	15.094808438013729
31	15.356331647571123	18.504398458970723	50.99602193582391	15.143247957634243
32	15.488097180217602	19.597675906986083	49.462020153851945	15.45220675894437
33	16.06610865009349	20.315484332450715	47.14269579730696	16.47571122014883
34	15.607062632549852	21.365090918217525	45.608694015335	17.419152433897626
35	16.09095586482111	22.563529810383123	42.94778320177695	18.39773112301881
36	16.986710504850226	24.42431011331836	40.96678253667474	17.622196845156676
37	17.18071956529923	24.567871798411282	42.242523874659604	16.008884761629876
38	16.47972693161996	25.843864118363097	40.21860529320968	17.457803656807258
39	18.676070124361566	23.609120684678807	39.915419077139305	17.79939011382032
40	18.74785096690803	23.566202768331095	40.267797758731035	17.418148506029844
41	15.949653017430698	25.14613425025412	39.11779838618595	19.78641434612923
42	16.60898264459699	24.66675869338788	39.393125603925355	19.331133058089776
43	17.541380651800168	24.086488385809478	39.617754464341736	18.754376498048615
44	16.950318119643104	23.94543652038601	39.62628785121789	19.477957508752997
45	17.773789953191862	23.32977775546827	39.10299045013616	19.79344184120371
46	17.67264422051276	23.683913310828615	39.99196857705774	18.65147389160089
47	16.55351562990199	22.6925345413932	42.40741902694292	18.346530801761894
48	16.177293661450427	21.909470804522694	42.21893156976671	19.69430396426017
49	16.29299634821238	21.518440900021332	43.16613751301969	19.022425238746596
50	15.571674175210513	21.001167066146298	43.11845093930001	20.30870781934318
51	15.555611329325988	21.180368190545508	41.3578124411761	21.9062080389524
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	112.0
1	261.0
2	410.0
3	24890.0
4	49370.0
5	32486.0
6	15602.0
7	14752.0
8	13902.0
9	13384.0
10	12866.0
11	12196.0
12	11526.0
13	10759.5
14	9993.0
15	9164.5
16	8336.0
17	7602.0
18	6868.0
19	6157.5
20	5447.0
21	5003.5
22	4560.0
23	4240.0
24	3920.0
25	3876.0
26	3986.0
27	4140.0
28	4066.0
29	3992.0
30	4262.5
31	4533.0
32	5137.5
33	5742.0
34	6416.0
35	7090.0
36	7660.5
37	8231.0
38	9044.0
39	9857.0
40	10944.0
41	12031.0
42	12981.5
43	13932.0
44	16035.0
45	18138.0
46	20445.5
47	22753.0
48	25006.5
49	27260.0
50	26755.0
51	26250.0
52	22760.5
53	19271.0
54	16796.5
55	14322.0
56	13416.5
57	12511.0
58	11683.5
59	10856.0
60	10210.0
61	9564.0
62	8675.5
63	7787.0
64	6539.0
65	5291.0
66	4137.5
67	2984.0
68	2497.5
69	2011.0
70	1662.0
71	1313.0
72	1058.0
73	803.0
74	678.0
75	421.5
76	290.0
77	187.5
78	85.0
79	66.5
80	48.0
81	39.5
82	31.0
83	25.0
84	19.0
85	10.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398435.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.97686544559466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1327007674704	34.43852298675676
2	8.264873915533212	7.10396748385352
3	2.76749250904321	3.5681445954852364
4	1.4168140547738108	2.435609079737658
5	0.9614486630495318	2.0660024912363304
6	0.703043136142448	1.8128754158665723
7	0.6053645739018818	1.8211670286677646
8	0.5296164942254394	1.8209005448075422
9	0.46807484333149174	1.810475060429279
>10	4.011058839300713	29.147827086658534
>50	0.10242668085081723	3.000934439747137
>100	0.03355356786492288	2.6113341162594796
>500	0.0029432954267476215	0.898863603475095
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.886590853495243E-4	7.463376067019077
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29501	7.404219006864357	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	941	0.23617403089588015	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	895	0.22462886041637908	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	604	0.15159310803518766	No Hit
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	579	0.1453185588615458	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	534	0.13402437034899042	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	499	0.1252400015058918	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	415	0.1041575162824551	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.019639338913499E-4	0.0	0.0	0.14958525229962227	0.0
2	5.019639338913499E-4	0.0	0.0	0.7923500696474959	0.0
3	5.019639338913499E-4	0.0	0.0	1.0947833398170341	0.0
4	5.019639338913499E-4	0.0	0.0	1.4742680738388947	0.0
5	5.019639338913499E-4	0.0	0.0	2.521113857969305	0.0
6	5.019639338913499E-4	0.0	0.0	3.0255876115301117	0.0
7	5.019639338913499E-4	0.0	0.0	3.561936074893019	0.0
8	5.019639338913499E-4	0.0	0.0	4.365078369119178	0.0
9	5.019639338913499E-4	0.0	0.0	4.645676208164444	0.0
10	5.019639338913499E-4	0.0	0.0	5.372018020505227	0.0
11	5.019639338913499E-4	0.0	0.0	6.381216509593786	0.0
12	5.019639338913499E-4	0.0	0.0	7.223261008696525	0.0
13	5.019639338913499E-4	0.0	0.0	7.575388708321307	0.0
14	5.019639338913499E-4	0.0	0.0	7.72146021308369	0.0
15	5.019639338913499E-4	0.0	0.0	8.016364024244858	0.0
16	5.019639338913499E-4	0.0	0.0	8.569779261360072	0.0
17	7.529459008370249E-4	0.0	0.0	9.203508727897901	0.0
18	7.529459008370249E-4	0.0	0.0	9.916046532056672	0.0
19	7.529459008370249E-4	0.0	0.0	10.337696236525405	0.0
20	0.0010039278677826998	0.0	0.0	10.741024257407105	0.0
21	0.0010039278677826998	0.0	0.0	11.234454804422302	0.0
22	0.0010039278677826998	0.0	0.0	11.75398747599985	0.0
23	0.0010039278677826998	0.0	0.0	12.269504436106265	0.0
24	0.0012549098347283748	0.0	0.0	12.708722878261197	0.0
25	0.0015058918016740497	0.0	0.0	13.086952702448329	0.0
26	0.0015058918016740497	0.0	0.0	13.432554870932524	0.0
27	0.0017568737686197247	0.0	0.0	13.745278401746834	0.0
28	0.0017568737686197247	0.0	0.0	14.085358966958225	0.0
29	0.0017568737686197247	0.0	0.0	14.416153199392623	0.0
30	0.0017568737686197247	0.0	0.0	14.837802903861357	0.0
31	0.0017568737686197247	0.0	0.0	15.20724835920539	0.0
32	0.0017568737686197247	0.0	0.0	15.567909445706325	0.0
33	0.0017568737686197247	0.0	0.0	15.91526848795914	0.0
34	0.0017568737686197247	0.0	0.0	16.252086287600235	0.0
35	0.0017568737686197247	0.0	0.0	16.62730432818402	0.0
36	0.0017568737686197247	0.0	0.0	16.991981126156087	0.0
37	0.0017568737686197247	0.0	0.0	17.329300889731073	0.0
38	0.0017568737686197247	0.0	0.0	17.67766385985167	0.0
39	0.0017568737686197247	0.0	0.0	18.016238533261387	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGT	20	7.027731E-4	45.000004	28
GGTACAC	20	7.027731E-4	45.000004	8
TACCAAG	20	7.027731E-4	45.000004	29
TTTCGCG	20	7.027731E-4	45.000004	1
CGCGACC	20	7.027731E-4	45.000004	10
GCTGACG	20	7.027731E-4	45.000004	1
GACCCAC	30	2.161918E-6	45.000004	12
TGCGAGG	60	0.0	45.000004	2
TCAGACG	20	7.027731E-4	45.000004	22
GGTGCGT	20	7.027731E-4	45.000004	9
CAAGCGC	20	7.027731E-4	45.000004	27
TAGGCGG	20	7.027731E-4	45.000004	2
CAGTCCC	20	7.027731E-4	45.000004	38
GCGTAAG	20	7.027731E-4	45.000004	1
GTACCGG	25	3.8862017E-5	45.0	2
TCGATGG	25	3.8862017E-5	45.0	2
GCGGGTA	45	3.8380676E-10	45.0	5
GGTATTA	25	3.8862017E-5	45.0	8
CGAATGG	25	3.8862017E-5	45.0	2
CGTTTTT	15240	0.0	44.542324	1
>>END_MODULE