##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935696.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	216208
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.408315140975354	33.0	31.0	34.0	30.0	34.0
2	32.595916894842006	34.0	31.0	34.0	31.0	34.0
3	32.4467133501073	34.0	31.0	34.0	30.0	34.0
4	36.069595019610745	37.0	35.0	37.0	35.0	37.0
5	36.113807074668834	37.0	35.0	37.0	35.0	37.0
6	36.13463424110116	37.0	36.0	37.0	35.0	37.0
7	36.25407477984163	37.0	37.0	37.0	35.0	37.0
8	36.01569322134241	37.0	37.0	37.0	35.0	37.0
9	37.79912399171169	39.0	38.0	39.0	35.0	39.0
10	37.69459039443499	39.0	38.0	39.0	35.0	39.0
11	37.69687060608303	39.0	38.0	39.0	35.0	39.0
12	37.627599348775256	39.0	37.0	39.0	35.0	39.0
13	37.57117220454377	39.0	37.0	39.0	35.0	39.0
14	38.97994523791904	41.0	38.0	41.0	35.0	41.0
15	39.07754107156072	41.0	39.0	41.0	35.0	41.0
16	39.13245578331976	41.0	39.0	41.0	35.0	41.0
17	39.101809368756015	41.0	39.0	41.0	35.0	41.0
18	38.44572818767113	39.0	38.0	40.0	35.0	41.0
19	37.756456745356324	37.0	37.0	40.0	35.0	41.0
20	36.94798064826463	36.0	35.0	40.0	34.0	41.0
21	36.88651668763413	36.0	35.0	40.0	34.0	41.0
22	36.89626193295345	36.0	35.0	40.0	34.0	41.0
23	36.914443498852954	36.0	35.0	40.0	34.0	41.0
24	36.74447291497077	35.0	35.0	40.0	34.0	41.0
25	36.648051875971284	35.0	35.0	40.0	34.0	41.0
26	36.504167283356765	35.0	35.0	40.0	33.0	41.0
27	36.43700048101828	35.0	35.0	40.0	33.0	41.0
28	36.288245578331974	35.0	35.0	40.0	33.0	41.0
29	36.02666413823725	35.0	35.0	40.0	33.0	41.0
30	35.54086342781026	35.0	35.0	40.0	31.0	41.0
31	34.90660382594539	35.0	35.0	40.0	25.0	41.0
32	33.915017020646786	35.0	34.0	40.0	18.0	41.0
33	32.76256197735514	35.0	33.0	40.0	12.0	41.0
34	31.809109746170353	35.0	30.0	40.0	8.0	41.0
35	31.251008288314956	35.0	25.0	40.0	7.0	41.0
36	30.921779027603048	35.0	23.0	40.0	7.0	41.0
37	30.753431880411455	35.0	23.0	40.0	7.0	41.0
38	30.60988955080293	35.0	23.0	40.0	7.0	41.0
39	30.502039702508696	35.0	23.0	40.0	7.0	41.0
40	30.354098830755568	35.0	22.0	40.0	7.0	41.0
41	30.18003496632872	35.0	22.0	40.0	7.0	41.0
42	30.050044401687263	35.0	21.0	40.0	7.0	41.0
43	29.898153629837935	35.0	20.0	40.0	7.0	41.0
44	29.79313901428254	35.0	20.0	40.0	7.0	41.0
45	29.73594871605121	35.0	20.0	40.0	7.0	41.0
46	29.654170983497373	35.0	20.0	40.0	7.0	41.0
47	29.692661696144455	35.0	20.0	40.0	7.0	41.0
48	29.654739880115443	35.0	20.0	40.0	7.0	41.0
49	29.608011729445717	35.0	20.0	40.0	7.0	41.0
50	29.502705727817656	35.0	20.0	40.0	7.0	41.0
51	28.87444035373344	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	4.0
10	2.0
11	1.0
12	3.0
13	5.0
14	4.0
15	16.0
16	20.0
17	42.0
18	105.0
19	196.0
20	334.0
21	523.0
22	842.0
23	1469.0
24	3006.0
25	6451.0
26	11272.0
27	13468.0
28	12245.0
29	9364.0
30	7148.0
31	6099.0
32	5869.0
33	6310.0
34	10155.0
35	16036.0
36	15340.0
37	13747.0
38	25137.0
39	50926.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.49115666395323	13.085084733219862	14.12667431362392	46.29708428920299
2	58.0829571523718	14.900003700140605	15.39767261155924	11.619366535928364
3	19.791127062828387	15.00314511951454	53.7519425738178	11.453785243839267
4	19.200029601124843	16.439724709538964	52.130355953526234	12.22988973580996
5	16.294956708354917	19.048786353881447	51.49855694516392	13.157699992599719
6	16.454525271960335	21.823429290313033	51.374139717309255	10.347905720417376
7	49.03842596018649	4.6163879227410645	42.66909642566418	3.6760896914082735
8	48.24937097609709	4.241286168874417	42.99794642196403	4.511396433064457
9	43.60569451639162	6.0483423370088065	44.785114334344705	5.560848812254866
10	24.90610893213942	16.417061348331234	49.34970028861096	9.327129430918376
11	19.543680159846076	16.2246540368534	51.95691186265078	12.274753940649745
12	19.476152593798567	13.791811588840375	54.56366091911493	12.168374898246133
13	15.698771553319027	16.51048989861615	55.040516539628506	12.750222008436321
14	11.645267520165767	19.40723747502405	54.390679345815144	14.556815658995042
15	11.531025678975801	16.588655368904018	59.14952268186191	12.73079627025827
16	12.042107600088803	16.17932731443795	58.1375342263006	13.641030859172648
17	12.574465329682527	15.688133649078665	54.52388440760749	17.21351661363132
18	13.894953008214312	17.098812254865685	55.194072374750235	13.812162362169763
19	15.661307629689928	17.645970546880783	53.34538962480574	13.34733219862355
20	16.97300747428402	15.757973803004512	54.23758602826907	13.031432694442389
21	14.898153629837935	17.28520683785984	54.100680825871386	13.715958706430845
22	12.97870569081625	17.239880115444386	53.32180122844669	16.45961296529268
23	13.622067638570265	17.018334196699474	54.376803818545106	14.982794346185155
24	14.14101235846962	15.405072892769924	55.578886997705915	14.87502775105454
25	13.037445422926073	16.846740176126694	54.03731591800488	16.07849848294235
26	12.075408865536891	18.348072226744616	54.43785613853327	15.13866276918523
27	13.993931769407236	16.970232368830015	54.847646710575006	14.188189151187744
28	11.635092133501072	16.93184341004958	55.79349515281581	15.639569303633538
29	12.414434248501443	16.307444682897952	55.155683415969804	16.1224376526308
30	13.000444016872642	16.384222600458816	53.547047287796936	17.068286094871603
31	13.58876637312218	17.908680529860135	50.536057870199066	17.96649522681862
32	14.178938799674388	19.512691482276328	49.27662251165545	17.031747206393845
33	14.116498926959226	21.526030489158586	46.10143935469548	18.25603122918671
34	13.478224672537555	21.82204173758603	45.021923333086654	19.67781025678976
35	14.259879375416265	21.660160586102272	43.79810182786946	20.281858210612004
36	14.858377118330496	25.08972840967957	41.430474358025606	18.62142011396433
37	15.083623177680753	25.19795752238585	41.976707614889364	17.74171168504403
38	14.944867904980388	25.206282838747875	39.73534744320284	20.113501813068897
39	15.983219862354769	23.41726485606453	40.29638126248798	20.303134019092724
40	16.517427662251166	22.60785909864575	41.89484200399615	18.979871235106934
41	13.551764967068749	24.51250647524606	41.24176718715311	20.69396137053208
42	15.430511359431659	25.09712869089025	40.284818323096275	19.18754162658181
43	16.947569007622292	24.06663953230223	39.72008436320581	19.26570709686968
44	15.389347295197217	24.51713165100274	38.836213276104495	21.25730777769555
45	15.768611707244876	22.82524235920965	38.7899615185377	22.61618441500777
46	15.595167616369421	23.499130466957745	40.50451417153852	20.401187745134315
47	14.713146599570784	22.44459039443499	42.80600162806187	20.03626137793236
48	14.551727965662694	20.96592170502479	43.02893509953378	21.453415229778734
49	14.668282394731	21.16665433286465	43.33188411159624	20.83317916080811
50	14.157662991193664	20.47611559239251	43.36287278916599	22.003348627247835
51	13.81493746762377	20.486290979057202	41.130300451417156	24.568471101901874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	161.5
2	234.0
3	13721.5
4	27209.0
5	17865.5
6	8522.0
7	8031.5
8	7541.0
9	7347.0
10	7153.0
11	6776.5
12	6400.0
13	5935.0
14	5470.0
15	4938.5
16	4407.0
17	4074.0
18	3741.0
19	3470.0
20	3199.0
21	3013.5
22	2828.0
23	2549.0
24	2270.0
25	2088.5
26	1999.0
27	2091.0
28	2091.0
29	2091.0
30	2416.5
31	2742.0
32	2826.0
33	2910.0
34	3161.5
35	3413.0
36	3817.5
37	4222.0
38	4338.5
39	4455.0
40	5055.0
41	5655.0
42	6560.0
43	7465.0
44	8506.0
45	9547.0
46	11120.5
47	12694.0
48	14475.5
49	16257.0
50	15512.0
51	14767.0
52	12469.5
53	10172.0
54	9053.5
55	7935.0
56	6982.5
57	6030.0
58	5698.0
59	5366.0
60	5503.5
61	5641.0
62	4965.5
63	4290.0
64	3646.0
65	3002.0
66	2340.5
67	1679.0
68	1530.0
69	1381.0
70	991.5
71	602.0
72	480.0
73	358.0
74	311.5
75	179.5
76	94.0
77	70.5
78	47.0
79	39.0
80	31.0
81	20.5
82	10.0
83	11.5
84	13.0
85	7.0
86	1.0
87	6.5
88	12.0
89	6.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	216208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.53485532450233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68257998984257	36.73869607045068
2	7.92280345352971	7.215274180418856
3	2.8176739461655664	3.8490712647080585
4	1.3743016759776536	2.5031451195145418
5	0.9537836465210767	2.171520017760675
6	0.808532249873032	2.2089839413897727
7	0.6307770441848654	2.010563901428254
8	0.5942102590147282	2.1645822541256567
9	0.4966988318943626	2.0355398505143194
>10	3.6201117318435756	26.71594020572782
>50	0.07211782630777044	2.1511692444312884
>100	0.02336211274758761	1.9758750832531635
>500	0.002031488065007618	0.6341115962406572
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.001015744032503809	7.625527270036262
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16487	7.625527270036262	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	713	0.32977503145119513	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	658	0.304336564789462	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	366	0.1692814326944424	TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	299	0.13829275512469474	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	270	0.12487974543032634	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	253	0.11701694664397247	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	225	0.10406645452527197	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	221	0.10221638422260046	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.250351513357507E-4	0.0	0.0	0.1785317842077999	0.0
2	9.250351513357507E-4	0.0	0.0	1.0143010434396507	0.0
3	9.250351513357507E-4	0.0	0.0	1.4546177754754681	0.0
4	9.250351513357507E-4	0.0	0.0	1.907422482054318	0.0
5	9.250351513357507E-4	0.0	0.0	3.151132243025235	0.0
6	9.250351513357507E-4	0.0	0.0	3.8268704210760007	0.0
7	9.250351513357507E-4	0.0	0.0	4.499370976097092	0.0
8	9.250351513357507E-4	0.0	0.0	5.620513579516022	0.0
9	9.250351513357507E-4	0.0	0.0	6.063605417005847	0.0
10	9.250351513357507E-4	0.0	0.0	6.889661807148672	0.0
11	9.250351513357507E-4	0.0	0.0	8.099607785095834	0.0
12	9.250351513357507E-4	0.0	0.0	9.085695256419744	0.0
13	9.250351513357507E-4	0.0	0.0	9.526011988455561	0.0
14	9.250351513357507E-4	0.0	0.0	9.679105306001627	0.0
15	0.0013875527270036261	0.0	0.0	10.022755864722859	0.0
16	0.0013875527270036261	0.0	0.0	10.706356841559979	0.0
17	0.0013875527270036261	0.0	0.0	11.430659365055872	0.0
18	0.0013875527270036261	0.0	0.0	12.259490860652704	0.0
19	0.0013875527270036261	0.0	0.0	12.663731221786428	0.0
20	0.0013875527270036261	0.0	0.0	13.134574113816326	0.0
21	0.0013875527270036261	0.0	0.0	13.710871013098497	0.0
22	0.0013875527270036261	0.0	0.0	14.307981203285724	0.0
23	0.0013875527270036261	0.0	0.0	14.890753348627248	0.0
24	0.0013875527270036261	0.0	0.0	15.32783245763339	0.0
25	0.0013875527270036261	0.0	0.0	15.705246799378376	0.0
26	0.0013875527270036261	0.0	0.0	16.103474432028417	0.0
27	0.0013875527270036261	0.0	0.0	16.49060164286243	0.0
28	0.0013875527270036261	0.0	0.0	16.856915562791386	0.0
29	0.0013875527270036261	0.0	0.0	17.238030045141716	0.0
30	0.0013875527270036261	0.0	0.0	17.656145933545474	0.0
31	0.0013875527270036261	0.0	0.0	18.05761118922519	0.0
32	0.0013875527270036261	0.0	0.0	18.448901058240214	0.0
33	0.0013875527270036261	0.0	0.0	18.809664767261157	0.0
34	0.0013875527270036261	0.0	0.0	19.16580330052542	0.0
35	0.0013875527270036261	0.0	0.0	19.532579738030044	0.0
36	0.0013875527270036261	0.0	0.0	19.951158144009472	0.0
37	0.0013875527270036261	0.0	0.0	20.33504773181381	0.0
38	0.0013875527270036261	0.0	0.0	20.685636054170057	0.0
39	0.0013875527270036261	0.0	0.0	21.042237105009992	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATTC	70	0.0	45.0	43
CGGGTAG	20	7.0205715E-4	45.0	6
TCGTTCC	20	7.0205715E-4	45.0	13
CAACGAG	20	7.0205715E-4	45.0	14
CTATCCA	20	7.0205715E-4	45.0	28
GAGTCCA	20	7.0205715E-4	45.0	36
ATAATAC	20	7.0205715E-4	45.0	23
GCTATCC	20	7.0205715E-4	45.0	27
ATGCTTT	70	0.0	45.0	38
CGACAGG	20	7.0205715E-4	45.0	2
CCGCTAT	20	7.0205715E-4	45.0	25
CGTTCCT	20	7.0205715E-4	45.0	14
GGCCCGC	20	7.0205715E-4	45.0	22
TATGAGG	20	7.0205715E-4	45.0	2
TATTGCG	20	7.0205715E-4	45.0	1
CAGGGCG	20	7.0205715E-4	45.0	5
AACGGGA	20	7.0205715E-4	45.0	4
CGCTATC	20	7.0205715E-4	45.0	26
CTACGAA	70	0.0	45.0	11
CGCTAGG	20	7.0205715E-4	45.0	2
>>END_MODULE