##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935695.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	600369
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48948230171778	34.0	31.0	34.0	31.0	34.0
2	32.6829299980512	34.0	31.0	34.0	31.0	34.0
3	32.52926783361566	34.0	31.0	34.0	31.0	34.0
4	36.12298436461576	37.0	37.0	37.0	35.0	37.0
5	36.154973024923	37.0	35.0	37.0	35.0	37.0
6	36.15949524375842	37.0	37.0	37.0	35.0	37.0
7	36.28390373253782	37.0	37.0	37.0	35.0	37.0
8	36.02873399525958	37.0	37.0	37.0	35.0	37.0
9	37.849615819604274	39.0	38.0	39.0	35.0	39.0
10	37.732121411998286	39.0	38.0	39.0	35.0	39.0
11	37.72265223554181	39.0	38.0	39.0	35.0	39.0
12	37.6719300963241	39.0	38.0	39.0	35.0	39.0
13	37.685198602859245	39.0	38.0	39.0	35.0	39.0
14	39.13329469043205	41.0	39.0	41.0	35.0	41.0
15	39.224525250304396	41.0	39.0	41.0	35.0	41.0
16	39.265701593519985	41.0	39.0	41.0	35.0	41.0
17	39.22724357853254	41.0	39.0	41.0	35.0	41.0
18	38.543122646239226	39.0	39.0	41.0	35.0	41.0
19	37.83130374819486	37.0	37.0	41.0	35.0	41.0
20	37.01402304249553	36.0	35.0	41.0	34.0	41.0
21	36.961375420782886	36.0	35.0	40.0	34.0	41.0
22	36.94418099535452	36.0	35.0	40.0	34.0	41.0
23	36.96890578960606	36.0	35.0	40.0	34.0	41.0
24	36.80204507561183	36.0	35.0	40.0	34.0	41.0
25	36.7096652225548	35.0	35.0	40.0	34.0	41.0
26	36.54247471138583	35.0	35.0	40.0	33.0	41.0
27	36.46978275027525	35.0	35.0	40.0	33.0	41.0
28	36.32755188892165	35.0	35.0	40.0	33.0	41.0
29	36.05431826093619	35.0	35.0	40.0	33.0	41.0
30	35.52937276908035	35.0	35.0	40.0	31.0	41.0
31	34.877167208833235	35.0	35.0	40.0	24.0	41.0
32	33.83725342247851	35.0	34.0	40.0	18.0	41.0
33	32.68444739818345	35.0	33.0	40.0	12.0	41.0
34	31.70619735529316	35.0	30.0	40.0	8.0	41.0
35	31.110332145730375	35.0	24.0	40.0	7.0	41.0
36	30.741334079541083	35.0	23.0	40.0	7.0	41.0
37	30.552385283050924	35.0	23.0	40.0	7.0	41.0
38	30.37738290951065	35.0	22.0	40.0	7.0	41.0
39	30.244547936352475	35.0	21.0	40.0	7.0	41.0
40	30.113821666341867	35.0	21.0	40.0	7.0	41.0
41	29.965109790811983	35.0	21.0	40.0	7.0	41.0
42	29.841765647460146	35.0	20.0	40.0	7.0	41.0
43	29.718628043753093	35.0	18.0	40.0	7.0	41.0
44	29.61001650651516	35.0	18.0	40.0	7.0	41.0
45	29.58422903247836	35.0	20.0	40.0	7.0	41.0
46	29.569065025009618	35.0	20.0	40.0	7.0	41.0
47	29.59990938905906	35.0	20.0	40.0	7.0	41.0
48	29.564334600887122	35.0	20.0	40.0	7.0	41.0
49	29.5324342196216	35.0	20.0	40.0	7.0	41.0
50	29.425699861251996	35.0	20.0	40.0	7.0	41.0
51	28.856624842388598	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	7.0
10	13.0
11	10.0
12	15.0
13	12.0
14	19.0
15	26.0
16	61.0
17	136.0
18	313.0
19	549.0
20	988.0
21	1621.0
22	2507.0
23	4162.0
24	8098.0
25	16951.0
26	30440.0
27	39602.0
28	36260.0
29	27352.0
30	19936.0
31	17046.0
32	15756.0
33	16664.0
34	26160.0
35	39512.0
36	42793.0
37	39366.0
38	71635.0
39	142197.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.15874570472493	12.822780656562879	14.265726578154435	46.75274706055776
2	59.92547916364769	14.49591834355205	14.567374398078513	11.011228094721746
3	21.040726619795493	14.665147600892118	53.357018766791754	10.937107012520633
4	19.05344879565734	15.963682335363751	52.944772298369834	12.038096570609074
5	16.297310487383594	18.4871304147949	52.32998372667477	12.885575371146746
6	16.502850746790724	21.609376899873244	52.37445637599543	9.513315977340604
7	49.19224676823754	4.262045508678829	43.19826639949764	3.3474413235859943
8	48.914584197385274	4.450762780889753	43.010881641124044	3.6237713806009304
9	44.98833217571194	5.8337455798017555	44.53161305796935	4.646309186516959
10	24.62202412183174	16.722882094178747	49.08264750511769	9.572446278871826
11	19.033127959638154	16.18687840311542	52.4044379373352	12.37555569991122
12	18.35437872375156	14.219588286537114	54.67270961691892	12.7533233727924
13	15.809110730234238	15.187992717811879	55.52235375244224	13.480542799511634
14	12.64672226580653	17.490076936017683	55.07446253887193	14.78873825930386
15	12.171014825882082	17.070001948801487	57.439508035891265	13.31947518942517
16	13.882462285694297	15.87307139442576	56.77175203916258	13.47271428071736
17	13.848649747072217	16.05312732669408	54.980520313340634	15.117702612893073
18	14.358169725618744	16.94074810658112	54.9883488321349	13.712733335665234
19	15.64820968437744	16.97306156713621	54.070579926678434	13.308148821807922
20	16.605287748034957	16.14490421723973	54.51763831910043	12.732169715624892
21	15.928370718674682	16.528668202388864	54.37922344424846	13.163737634688
22	14.35800316138908	16.092936177584118	54.20116628273611	15.347894378290686
23	15.011268070136866	16.14490421723973	54.99784299322583	13.845984719397569
24	14.852865487725051	15.86074564143052	55.076128181168585	14.210260689675849
25	14.689799106882601	16.79067373565257	53.87386757144357	14.645659586021264
26	13.625620243550216	17.88999765144436	53.83539123439085	14.648990870614572
27	14.511741945370263	17.287867961203858	54.47333223400942	13.727057859416458
28	13.529679247262932	17.479916518008093	54.788971449225386	14.201432785503581
29	14.28521459302529	17.58834983152028	53.79458299812282	14.331852577331608
30	14.445949074652424	17.5858513680753	53.249917967116886	14.718281590155389
31	15.656204767401382	18.746804048843295	50.593884760872065	15.003106422883262
32	16.276489958675413	20.24688150121009	49.01951966207449	14.457108878040007
33	16.505848902924704	21.5840591369641	46.54604085154297	15.36405110856823
34	16.425731508455634	22.764666396832613	44.05890377417888	16.750698320532873
35	17.0323584328971	23.029336957770973	42.53134322391729	17.406961385414636
36	17.362988428782966	25.865592660513787	40.384663431989324	16.386755478713923
37	18.281590155387768	25.697529352781373	40.31437332707052	15.706507164760339
38	18.196642398258405	25.54845436723082	39.415759308025564	16.83914392648521
39	18.656859364823966	24.89202473811939	39.046153282397995	17.40496261465865
40	17.694950938506153	25.32825645561313	40.42946920976932	16.54732339611139
41	16.34478129283824	26.004174099595417	39.74472366161477	17.906320945951574
42	17.394135939730397	26.384440235921574	38.7373432005983	17.484080623749726
43	18.276926356957137	25.254635066101017	38.90790497177569	17.560533605166157
44	17.44443833708936	25.740169795575724	38.72235241992841	18.09303944740651
45	17.400465380457685	24.34369529406082	38.932723041995835	19.323116283485657
46	16.94907631806439	24.926003840971138	39.95725961866785	18.16766022229662
47	17.171273000438063	23.7527254072079	41.8973997658107	17.178601826543343
48	16.76718817926975	23.05049061493848	42.076622876930685	18.105698328861084
49	16.67474503180544	22.725357238631574	42.54233646307521	18.057561266487777
50	15.660035744683686	22.211173461654415	43.19393572952634	18.934855064135554
51	15.883065248205686	21.935509661558143	41.89490130236571	20.286523787870458
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	154.0
1	372.5
2	591.0
3	39283.0
4	77975.0
5	51941.5
6	25908.0
7	24484.0
8	23060.0
9	21802.0
10	20544.0
11	19306.5
12	18069.0
13	16866.0
14	15663.0
15	14212.5
16	12762.0
17	11671.5
18	10581.0
19	9758.5
20	8936.0
21	8089.0
22	7242.0
23	6561.5
24	5881.0
25	5668.5
26	5482.5
27	5509.0
28	5878.5
29	6248.0
30	6662.5
31	7077.0
32	7571.0
33	8065.0
34	9046.0
35	10027.0
36	10678.0
37	11329.0
38	12256.0
39	13183.0
40	15181.0
41	17179.0
42	18455.0
43	19731.0
44	21626.0
45	23521.0
46	26505.5
47	29490.0
48	32140.5
49	34791.0
50	34703.5
51	34616.0
52	30471.5
53	26327.0
54	24261.0
55	22195.0
56	20357.0
57	18519.0
58	18346.0
59	18173.0
60	17919.5
61	17666.0
62	16453.5
63	15241.0
64	12787.0
65	10333.0
66	8322.5
67	6312.0
68	5405.5
69	4499.0
70	3854.0
71	3209.0
72	2592.5
73	1976.0
74	1534.5
75	860.0
76	627.0
77	457.0
78	287.0
79	226.0
80	165.0
81	127.5
82	90.0
83	63.5
84	37.0
85	30.0
86	23.0
87	12.0
88	1.0
89	3.5
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	600369.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.3739476347147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96613909493993	32.285487123694175
2	7.625206241504257	6.157193549967852
3	2.620962241114232	3.1745577682593145
4	1.418382468660819	2.290627980628371
5	1.0080950385772816	2.035038814916743
6	0.7665523594537407	1.8569246891911397
7	0.6929652023249666	1.958441855394338
8	0.5844294260699742	1.8876578435468403
9	0.5267695226149806	1.91409886094588
>10	4.635103036931252	31.150048593630974
>50	0.09722597879477995	2.8148783996848854
>100	0.05401443265567154	3.600628722538182
>500	0.003739460722315722	1.078142042478238
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.15495635812858E-4	7.796273755123091
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46475	7.741072573700508	No Hit
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	985	0.16406576622044108	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	947	0.15773632549315506	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	816	0.1359164114069847	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	801	0.13341794796200335	No Hit
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	686	0.11426306155047979	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	612	0.10193730855523854	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3312845933084487E-4	0.0	0.0	0.09477504667962536	0.0
2	3.3312845933084487E-4	0.0	0.0	0.4707105130344838	0.0
3	3.3312845933084487E-4	0.0	0.0	0.6755845155229534	0.0
4	3.3312845933084487E-4	0.0	0.0	0.9345918926526853	0.0
5	3.3312845933084487E-4	0.0	0.0	1.6376595060704333	0.0
6	3.3312845933084487E-4	0.0	0.0	2.030584523851165	0.0
7	3.3312845933084487E-4	0.0	0.0	2.3951936225887747	0.0
8	3.3312845933084487E-4	0.0	0.0	2.9771690410397604	0.0
9	3.3312845933084487E-4	0.0	0.0	3.194202232293806	0.0
10	3.3312845933084487E-4	0.0	0.0	3.6620811534239777	0.0
11	3.3312845933084487E-4	0.0	0.0	4.358153069195778	0.0
12	3.3312845933084487E-4	0.0	0.0	4.95262080487167	0.0
13	3.3312845933084487E-4	0.0	0.0	5.182812570269284	0.0
14	4.996926889962673E-4	0.0	0.0	5.283250800757534	0.0
15	4.996926889962673E-4	0.0	0.0	5.470635559131134	0.0
16	4.996926889962673E-4	0.0	0.0	5.87905105027075	0.0
17	4.996926889962673E-4	0.0	0.0	6.290797826003675	0.0
18	6.662569186616897E-4	0.0	0.0	6.756677976377861	0.0
19	6.662569186616897E-4	0.0	0.0	7.044667529469376	0.0
20	6.662569186616897E-4	0.0	0.0	7.335655238694869	0.0
21	6.662569186616897E-4	0.0	0.0	7.69077017634155	0.0
22	6.662569186616897E-4	0.0	0.0	8.08952494216057	0.0
23	6.662569186616897E-4	0.0	0.0	8.455799683194835	0.0
24	6.662569186616897E-4	0.0	0.0	8.758613452726573	0.0
25	6.662569186616897E-4	0.0	0.0	9.028780633243889	0.0
26	6.662569186616897E-4	0.0	0.0	9.278293849282692	0.0
27	6.662569186616897E-4	0.0	0.0	9.523642959579858	0.0
28	8.328211483271122E-4	0.0	0.0	9.78814695628855	0.0
29	8.328211483271122E-4	0.0	0.0	10.043989613054638	0.0
30	8.328211483271122E-4	0.0	0.0	10.327648496174852	0.0
31	8.328211483271122E-4	0.0	0.0	10.615804613496033	0.0
32	8.328211483271122E-4	0.0	0.0	10.89979662507558	0.0
33	8.328211483271122E-4	0.0	0.0	11.168964420214902	0.0
34	9.993853779925346E-4	0.0	0.0	11.446127298378164	0.0
35	9.993853779925346E-4	0.0	0.0	11.728786796120385	0.0
36	9.993853779925346E-4	0.0	0.0	12.020274198034874	0.0
37	0.001165949607657957	0.0	0.0	12.302933695777098	0.0
38	0.001165949607657957	0.0	0.0	12.56044199483984	0.0
39	0.001165949607657957	0.0	0.0	12.811620853175297	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAG	70	0.0	45.0	1
CTCACGT	20	7.030592E-4	45.0	45
TACGGGT	35	1.2106284E-7	45.0	4
ACGATAG	20	7.030592E-4	45.0	1
TTGCACG	25	3.8885715E-5	45.0	1
TATACGG	20	7.030592E-4	45.0	2
CGATAGG	55	1.8189894E-12	45.0	2
GCGATTA	30	2.1637625E-6	44.999996	9
CGTTTTT	23220	0.0	44.389538	1
TGCACGG	65	0.0	41.538464	2
GTTTTTT	25650	0.0	40.34211	2
CGTAAGG	85	0.0	39.705883	2
CTACGAA	125	0.0	39.6	11
TACGAAT	125	0.0	39.6	12
CGAATAT	125	0.0	39.6	14
CGTATGG	35	6.243303E-6	38.571426	2
CGACGGT	35	6.243303E-6	38.571426	28
CGGTCTA	35	6.243303E-6	38.571426	31
ACGGTCT	35	6.243303E-6	38.571426	30
GCGCGAC	65	9.094947E-12	38.076927	9
>>END_MODULE