##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935694.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270437
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31313392768001	33.0	31.0	34.0	30.0	34.0
2	32.51746987283545	34.0	31.0	34.0	30.0	34.0
3	32.37913820963848	34.0	31.0	34.0	30.0	34.0
4	36.004026815857294	37.0	35.0	37.0	35.0	37.0
5	36.04543387184446	37.0	35.0	37.0	35.0	37.0
6	36.06093470937778	37.0	35.0	37.0	35.0	37.0
7	36.20396617326771	37.0	37.0	37.0	35.0	37.0
8	35.93739392168971	37.0	37.0	37.0	35.0	37.0
9	37.7691625036515	39.0	38.0	39.0	35.0	39.0
10	37.61650217980528	39.0	37.0	39.0	35.0	39.0
11	37.62676704740846	39.0	37.0	39.0	35.0	39.0
12	37.50282320836276	39.0	37.0	39.0	35.0	39.0
13	37.422150075618354	39.0	37.0	39.0	35.0	39.0
14	38.80617666961252	41.0	38.0	41.0	35.0	41.0
15	38.90009872909402	41.0	38.0	41.0	35.0	41.0
16	38.989365360509105	41.0	38.0	41.0	35.0	41.0
17	38.93507175423481	41.0	38.0	41.0	35.0	41.0
18	38.31293055314177	39.0	38.0	40.0	35.0	41.0
19	37.64892377892078	37.0	37.0	40.0	35.0	41.0
20	36.83816563561938	36.0	35.0	40.0	34.0	41.0
21	36.821145035627524	36.0	35.0	40.0	34.0	41.0
22	36.801798570461884	35.0	35.0	40.0	34.0	41.0
23	36.81836804875073	35.0	35.0	40.0	34.0	41.0
24	36.669679074978646	35.0	35.0	40.0	34.0	41.0
25	36.59322503947315	35.0	35.0	40.0	33.0	41.0
26	36.44024671180349	35.0	35.0	40.0	33.0	41.0
27	36.321246722896646	35.0	35.0	40.0	33.0	41.0
28	36.1906543853097	35.0	35.0	40.0	33.0	41.0
29	35.95970225967601	35.0	35.0	40.0	33.0	41.0
30	35.40972574019088	35.0	35.0	40.0	31.0	41.0
31	34.80963403676272	35.0	35.0	40.0	25.0	41.0
32	33.835303601208416	35.0	34.0	40.0	18.0	41.0
33	32.73968059104339	35.0	33.0	40.0	12.0	41.0
34	31.84406349722856	35.0	31.0	40.0	8.0	41.0
35	31.30485473511391	35.0	27.0	40.0	7.0	41.0
36	30.96406926567001	35.0	24.0	40.0	7.0	41.0
37	30.79848541434789	35.0	23.0	40.0	7.0	41.0
38	30.680472716381264	35.0	23.0	40.0	7.0	41.0
39	30.565222214415925	35.0	23.0	40.0	7.0	41.0
40	30.42929037077027	35.0	23.0	40.0	7.0	41.0
41	30.279861853222748	35.0	22.0	40.0	7.0	41.0
42	30.169444269829942	35.0	22.0	40.0	7.0	41.0
43	30.063863302728546	35.0	21.0	40.0	7.0	41.0
44	29.955124483706	35.0	21.0	40.0	7.0	41.0
45	29.918480089632705	35.0	22.0	40.0	7.0	41.0
46	29.89674489807238	35.0	22.0	40.0	7.0	41.0
47	29.90220642885404	35.0	22.0	40.0	7.0	41.0
48	29.905053672389503	35.0	22.0	40.0	7.0	41.0
49	29.876019183765536	35.0	22.0	40.0	7.0	41.0
50	29.734307805514778	35.0	20.0	40.0	7.0	41.0
51	29.104970843486655	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	8.0
11	4.0
12	7.0
13	6.0
14	1.0
15	22.0
16	31.0
17	63.0
18	132.0
19	245.0
20	410.0
21	760.0
22	1121.0
23	2029.0
24	3752.0
25	7681.0
26	13244.0
27	16564.0
28	14717.0
29	11694.0
30	9082.0
31	7785.0
32	7313.0
33	8322.0
34	13909.0
35	21647.0
36	19950.0
37	17966.0
38	32579.0
39	59345.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.595843024438224	13.268894419032899	14.982417346738796	45.15284520979008
2	56.26929747039051	16.986950750082276	15.072271915455357	11.671479864071854
3	20.025366351497762	16.572066692057668	52.507977828477614	10.894589127966956
4	19.24366858085247	15.268620787835985	53.60657010690105	11.88114052441049
5	17.390371879587484	18.59102119902232	50.94125434019753	13.077352581192661
6	15.958245358438381	22.469928301230972	51.81243690767165	9.759389432658992
7	48.86646427818679	4.026815857297633	43.30509508684093	3.8016247776746526
8	48.57841197765099	4.7242056375422	42.160651094339904	4.536731290466911
9	44.737961151765475	5.332110620957931	43.6123755255383	6.317552701738298
10	26.41798274644372	14.35417490949833	48.68379696565189	10.54404537840606
11	20.87732078081032	15.591061873929974	50.46239974559694	13.06921759966277
12	18.831372926041926	13.10212729767007	54.546160473603834	13.520339302684173
13	15.47199532608334	16.430444059059965	55.57967289978812	12.517887715068573
14	12.276056900498082	19.677780777038645	53.89610149498775	14.15006082747553
15	10.936743123167318	16.69483095878153	59.223035309517556	13.145390608533598
16	12.538594940781032	16.023325210677534	56.45936022067987	14.978719627861572
17	12.217263170350211	16.346136068659245	53.995200360897364	17.441400400093183
18	13.268894419032899	17.3149384144921	55.18216812048647	14.23399904598853
19	14.277262356852058	17.189955516441906	53.38914423692024	15.143637889785792
20	15.794066640289605	16.83460473234062	54.115376224407164	13.255952402962611
21	14.356393540824666	18.016765457389337	54.316162359440455	13.310678642345536
22	12.581488479756839	16.571327148282226	53.906455107843975	16.940729264116968
23	13.498892533196273	16.96069694605398	54.14421843164951	15.396192089100232
24	14.837466766751591	15.828455425847796	53.89573172310001	15.438346084300594
25	13.270003734696065	17.620000221863133	52.92286188650222	16.187134156938583
26	13.056645355480203	17.328250202450107	54.036614812322284	15.578489629747407
27	14.187038016247778	17.31345932694121	54.34167661969332	14.157826037117701
28	11.386755510525557	17.69580345884624	55.82483166134812	15.09260936928009
29	14.376361222761677	15.513040005620532	55.0246452963167	15.085953475301087
30	14.540539940910453	16.73254769132922	54.065087247676914	14.66182512008342
31	15.862474439518262	17.455081959938916	51.02186461172102	15.660578988821797
32	16.526214977980082	18.525201803007725	49.96098906584528	14.987594153166912
33	16.410846149010673	19.593842558525644	46.80313714469544	17.19217414776824
34	15.128847014276891	19.917762732170523	45.886102863143726	19.067287390408858
35	16.514012505685244	20.17549373791308	44.66141837100693	18.64907538539475
36	18.430540199750777	21.879772368425918	42.265666310453085	17.424021121370224
37	15.975994409049058	23.023107045263778	44.40516645281526	16.595732092871906
38	15.917200678901185	24.308803898874785	41.23363297181968	18.540362450404345
39	18.108099113656788	22.41002525541993	40.84980975236377	18.632065878559516
40	17.693584827519903	22.29576574211369	41.34382499436098	18.666824436005427
41	15.771510555138535	23.771525346014045	39.376638551677466	21.080325547169952
42	16.2910400573886	23.558536738685902	40.875324012616616	19.275099191308882
43	17.56453443870476	22.272839885074898	40.75182020211731	19.410805474103025
44	16.662660804549674	22.871500571297567	40.89344283511502	19.572395789037742
45	16.634188369195044	22.42518590281655	40.25188860991654	20.688737118071863
46	18.52779020622178	22.182615544470615	41.64851702984429	17.641077219463313
47	15.542251984750607	20.979377821821718	44.6987653316669	18.77960486176078
48	15.748954469987464	20.67801373332791	43.69927191915308	19.87375987753155
49	16.086925975365794	19.23442428365941	45.5762340212323	19.102415719742492
50	15.419487718026751	19.860817861461264	44.621113235245176	20.09858118526681
51	15.232013370951462	19.941797904872484	41.857068374519756	22.969120349656297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117.0
1	189.0
2	261.0
3	17359.5
4	34458.0
5	22433.5
6	10409.0
7	9917.5
8	9426.0
9	9064.5
10	8703.0
11	8366.0
12	8029.0
13	7515.5
14	7002.0
15	6223.5
16	5445.0
17	4895.5
18	4346.0
19	3855.0
20	3364.0
21	3003.0
22	2642.0
23	2435.0
24	2228.0
25	2225.5
26	2286.0
27	2349.0
28	2371.5
29	2394.0
30	2678.5
31	2963.0
32	2881.0
33	2799.0
34	3238.5
35	3678.0
36	3911.5
37	4145.0
38	4740.5
39	5336.0
40	6289.5
41	7243.0
42	8591.0
43	9939.0
44	11543.0
45	13147.0
46	16882.5
47	20618.0
48	21051.5
49	21485.0
50	20954.5
51	20424.0
52	16825.0
53	13226.0
54	11249.0
55	9272.0
56	8300.0
57	7328.0
58	6750.5
59	6173.0
60	5748.0
61	5323.0
62	4852.5
63	4382.0
64	4079.0
65	3776.0
66	3068.5
67	2361.0
68	1887.0
69	1413.0
70	1100.5
71	788.0
72	687.0
73	586.0
74	467.0
75	260.5
76	173.0
77	113.0
78	53.0
79	35.0
80	17.0
81	27.5
82	38.0
83	20.5
84	3.0
85	2.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	270437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.720009481703435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08442607773253	30.61794134699204
2	9.855627776697414	7.8293125747712216
3	3.3560957772769338	3.9991246828483393
4	1.6111959705402488	2.559868769069642
5	1.0037693425607113	1.9934863901977338
6	0.8222269734791467	1.9595317909622416
7	0.591987092504134	1.6459613049117174
8	0.5451436618543304	1.7322489134195627
9	0.5082687752845667	1.8169596516201105
>10	4.484723633163596	30.22169387531691
>50	0.0936808932642101	2.5647231565175304
>100	0.035877788909697485	2.567889970107921
>500	0.005979631484949581	2.0041971510184067
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	9.96605247491597E-4	8.487060422246621
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21440	7.927909272769629	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	986	0.36459508129434953	No Hit
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	976	0.3608973624171249	Illumina PCR Primer Index 5 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	883	0.3265085768589357	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	880	0.32539926119576834	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	823	0.30432226359558784	No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	515	0.19043252217706896	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	384	0.14199240488542617	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	376	0.13903422978364646	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	338	0.12498289805019283	Illumina PCR Primer Index 5 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	337	0.12461312616247039	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	322	0.11906654784663341	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	295	0.10908270687812688	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011093156631673921	0.0	0.0	0.20189545069646536	0.0
2	0.0011093156631673921	0.0	0.0	1.13150197643074	0.0
3	0.0011093156631673921	0.0	0.0	1.5878004858802606	0.0
4	0.0011093156631673921	0.0	0.0	2.117313829098829	0.0
5	0.0011093156631673921	0.0	0.0	3.688844351919301	0.0
6	0.0011093156631673921	0.0	0.0	4.3673757658900225	0.0
7	0.0011093156631673921	0.0	0.0	5.148703764647589	0.0
8	0.0011093156631673921	0.0	0.0	6.305720001331179	0.0
9	0.0011093156631673921	0.0	0.0	6.731697215987458	0.0
10	0.0011093156631673921	0.0	0.0	7.8990670655272766	0.0
11	0.0011093156631673921	0.0	0.0	9.19474776010679	0.0
12	0.0011093156631673921	0.0	0.0	10.61726021217511	0.0
13	0.0011093156631673921	0.0	0.0	11.098333438102035	0.0
14	0.0011093156631673921	0.0	0.0	11.27323554099476	0.0
15	0.0011093156631673921	0.0	0.0	11.66630305764374	0.0
16	0.0011093156631673921	0.0	0.0	12.311924773607162	0.0
17	0.0011093156631673921	0.0	0.0	13.059973302469706	0.0
18	0.0011093156631673921	0.0	0.0	13.955930586421237	0.0
19	0.0011093156631673921	0.0	0.0	14.52353043407522	0.0
20	0.0011093156631673921	0.0	0.0	15.052304233518342	0.0
21	0.0011093156631673921	0.0	0.0	15.653183551067347	0.0
22	0.0011093156631673921	0.0	0.0	16.227439292700332	0.0
23	0.0011093156631673921	0.0	0.0	16.761759670459295	0.0
24	0.0011093156631673921	0.0	0.0	17.22027681123515	0.0
25	0.0011093156631673921	0.0	0.0	17.60779774956829	0.0
26	0.0011093156631673921	0.0	0.0	17.959820586680078	0.0
27	0.0011093156631673921	0.0	0.0	18.328483158739374	0.0
28	0.00184885943861232	0.0	0.0	18.735602007121805	0.0
29	0.00184885943861232	0.0	0.0	19.09206210688626	0.0
30	0.00184885943861232	0.0	0.0	19.506206621135423	0.0
31	0.002588403214057248	0.0	0.0	19.921090679160027	0.0
32	0.002958175101779712	0.0	0.0	20.308241845605448	0.0
33	0.002958175101779712	0.0	0.0	20.67357647067524	0.0
34	0.002958175101779712	0.0	0.0	21.03928086763276	0.0
35	0.00369771887722464	0.0	0.0	21.432718156169457	0.0
36	0.00369771887722464	0.0	0.0	21.817650691288545	0.0
37	0.00369771887722464	0.0	0.0	22.172261931614386	0.0
38	0.004067490764947104	0.0	0.0	22.538336100459627	0.0
39	0.004067490764947104	0.0	0.0	22.897384603438137	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	30	2.1593241E-6	45.000004	25
ATGGGCG	30	2.1593241E-6	45.000004	5
CCTAGGG	35	1.2073178E-7	45.000004	3
CGTAAGG	35	1.2073178E-7	45.000004	2
TTTGCGG	30	2.1593241E-6	45.000004	2
CGGTCTA	30	2.1593241E-6	45.000004	31
TAGTGAG	30	2.1593241E-6	45.000004	1
CGCCGGT	35	1.2073178E-7	45.000004	28
TATACGG	30	2.1593241E-6	45.000004	2
CGGGTCT	20	7.02371E-4	45.0	6
CTATGCG	20	7.02371E-4	45.0	27
CGACGTT	20	7.02371E-4	45.0	27
ACGCACG	20	7.02371E-4	45.0	1
GACGTTT	20	7.02371E-4	45.0	28
TTTCGAC	20	7.02371E-4	45.0	24
TCGATCT	20	7.02371E-4	45.0	13
AGAGTGC	20	7.02371E-4	45.0	1
CGCCTAG	20	7.02371E-4	45.0	1
GACGGCC	20	7.02371E-4	45.0	35
ATTGGGC	20	7.02371E-4	45.0	4
>>END_MODULE