SRR2935692.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935692.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260542
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base quality graph

Per Sequence quality graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15199	5.833608400948791	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1368	0.5250592994603558	RNA PCR Primer, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1173	0.45021532037061207	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	909	0.34888808714142056	RNA PCR Primer, Index 16 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	609	0.23374350392643028	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	568	0.21800707755371493	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	527	0.20227065118099963	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	484	0.1857665942535177	RNA PCR Primer, Index 14 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	434	0.1665758303843526	RNA PCR Primer, Index 16 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	382	0.14661743596042096	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	332	0.12742667209125594	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	306	0.1174474748792901	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAT	40	6.7793735E-9	45.0	18
CGGGTAT	35	1.2070996E-7	45.0	6
TCGTTTG	20	7.023234E-4	45.0	1
AGCCGAA	20	7.023234E-4	45.0	26
TCGCAGG	20	7.023234E-4	45.0	2
CTGTAGT	20	7.023234E-4	45.0	5
ACGTTGA	45	3.8198777E-10	45.0	11
GGCACGT	50	2.1827873E-11	45.0	8
TTCACGT	20	7.023234E-4	45.0	30
TCGGGTA	20	7.023234E-4	45.0	5
CCTAAGG	20	7.023234E-4	45.0	17
TGGCCGG	25	3.8824743E-5	45.0	2
ACGATGG	20	7.023234E-4	45.0	2
AATGATC	25	3.8824743E-5	45.0	10
AACATAG	35	1.2070996E-7	45.0	1
CGGGATC	25	3.8824743E-5	45.0	6
GACGGGT	20	7.023234E-4	45.0	4
GTCTTTA	20	7.023234E-4	45.0	10
GGTCTTC	35	1.2070996E-7	45.0	8
ATCGGGC	20	7.023234E-4	45.0	4