SRR2935691.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935691.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376378
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24130	6.411107981869291	No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	627	0.16658784519817843	RNA PCR Primer, Index 40 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	526	0.1397531205330811	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	519	0.1378932881305496	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC	501	0.1331108619526115	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	466	0.12381169993995397	No Hit
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	436	0.11584098964339042	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	418	0.11105856346545229	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG	413	0.10973011174935836	No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	409	0.10866735037648322	Illumina PCR Primer Index 8 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATACG	20	7.0272345E-4	45.0	9
AGACGTG	20	7.0272345E-4	45.0	24
GTTACGG	25	3.8857892E-5	45.0	2
GTTAACG	20	7.0272345E-4	45.0	1
CGAGGGC	45	3.8380676E-10	45.0	4
GTATACG	20	7.0272345E-4	45.0	1
ATACGAG	20	7.0272345E-4	45.0	1
TATACGG	35	1.2090095E-7	45.0	2
AGTACTG	20	7.0272345E-4	45.0	39
CGTTTTT	14505	0.0	44.581177	1
TACGGCT	50	1.0768417E-9	40.5	7
GTTTTTT	16050	0.0	40.35981	2
CACGGGC	45	1.922308E-8	40.0	4
CGGGCTC	45	1.922308E-8	40.0	6
TCGATGG	35	6.237098E-6	38.571426	2
TTAACGG	35	6.237098E-6	38.571426	2
TACACGC	90	0.0	37.5	35
TAGTAGG	90	0.0	37.5	2
CGAATAT	30	1.13846385E-4	37.499996	14
GCTACGA	30	1.13846385E-4	37.499996	10