##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935691.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376378
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36696353134349	33.0	31.0	34.0	30.0	34.0
2	32.579133211824285	34.0	31.0	34.0	31.0	34.0
3	32.427716285223894	34.0	31.0	34.0	30.0	34.0
4	36.049434345259286	37.0	35.0	37.0	35.0	37.0
5	36.08361540791439	37.0	35.0	37.0	35.0	37.0
6	36.10075774885886	37.0	36.0	37.0	35.0	37.0
7	36.23638735526519	37.0	37.0	37.0	35.0	37.0
8	35.99225778339861	37.0	37.0	37.0	35.0	37.0
9	37.81558167586841	39.0	38.0	39.0	35.0	39.0
10	37.702185037382634	39.0	38.0	39.0	35.0	39.0
11	37.67021983218998	39.0	38.0	39.0	35.0	39.0
12	37.596235699217274	39.0	37.0	39.0	35.0	39.0
13	37.581989383013884	39.0	37.0	39.0	35.0	39.0
14	39.001726987230924	41.0	39.0	41.0	35.0	41.0
15	39.08306277200049	41.0	39.0	41.0	35.0	41.0
16	39.10942456785466	41.0	39.0	41.0	35.0	41.0
17	39.07270881932525	41.0	39.0	41.0	35.0	41.0
18	38.405084781788524	39.0	38.0	40.0	35.0	41.0
19	37.69859290394231	37.0	37.0	40.0	35.0	41.0
20	36.85761388816562	36.0	35.0	40.0	34.0	41.0
21	36.82139763748147	35.0	35.0	40.0	34.0	41.0
22	36.80404274426242	35.0	35.0	40.0	34.0	41.0
23	36.815443516889935	35.0	35.0	40.0	34.0	41.0
24	36.6486032658657	35.0	35.0	40.0	34.0	41.0
25	36.559235662020626	35.0	35.0	40.0	33.0	41.0
26	36.40407515848429	35.0	35.0	40.0	33.0	41.0
27	36.296924899967586	35.0	35.0	40.0	33.0	41.0
28	36.15342820249855	35.0	35.0	40.0	33.0	41.0
29	35.89112275425237	35.0	35.0	40.0	33.0	41.0
30	35.335333095983295	35.0	35.0	40.0	30.0	41.0
31	34.63207998342092	35.0	35.0	40.0	23.0	41.0
32	33.51968234062565	35.0	33.0	40.0	17.0	41.0
33	32.28751946181764	35.0	31.0	40.0	12.0	41.0
34	31.272638145693957	35.0	26.0	40.0	8.0	41.0
35	30.647798224125747	35.0	23.0	40.0	7.0	41.0
36	30.26422904633108	35.0	21.0	40.0	7.0	41.0
37	30.07934842100229	35.0	21.0	40.0	7.0	41.0
38	29.903413589529674	35.0	20.0	40.0	7.0	41.0
39	29.746015973303436	35.0	20.0	40.0	7.0	41.0
40	29.598555175913575	35.0	18.0	40.0	7.0	41.0
41	29.41436268857372	35.0	18.0	40.0	7.0	41.0
42	29.309080233169844	35.0	18.0	40.0	7.0	41.0
43	29.16552242692187	35.0	18.0	40.0	7.0	41.0
44	29.045541981731134	35.0	18.0	40.0	7.0	41.0
45	28.971175254664193	35.0	18.0	40.0	7.0	41.0
46	28.900241246831644	35.0	18.0	40.0	7.0	41.0
47	28.89669162384624	35.0	18.0	40.0	7.0	41.0
48	28.843686400374093	35.0	18.0	40.0	7.0	41.0
49	28.802315757031494	35.0	18.0	40.0	7.0	41.0
50	28.658521486378056	35.0	18.0	39.0	7.0	41.0
51	28.019690311335943	34.0	18.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	8.0
11	6.0
12	3.0
13	3.0
14	12.0
15	23.0
16	53.0
17	119.0
18	217.0
19	443.0
20	756.0
21	1137.0
22	1872.0
23	3217.0
24	6118.0
25	12455.0
26	20746.0
27	25214.0
28	22597.0
29	17483.0
30	13440.0
31	11032.0
32	10682.0
33	10949.0
34	16961.0
35	24808.0
36	24450.0
37	25481.0
38	45683.0
39	80323.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.193613866910393	13.2340359957277	14.802937472434627	45.76941266492728
2	58.93091519695625	15.558295118205635	14.878127839565543	10.63266184527257
3	19.858759013544894	15.765533585916286	53.80813968935485	10.56756771118397
4	18.010085605428586	16.069217648215357	53.76058111791869	12.160115628437369
5	16.267156953913354	18.6283470340987	52.43345785354086	12.671038158447093
6	16.02750426433001	21.713277609212014	52.52379257023524	9.735425556222733
7	48.95211728634511	3.997842594413063	43.579327165774835	3.470712953466993
8	49.11843944120007	4.053637566489009	43.248542688467445	3.579380303843477
9	45.64028715812295	5.100988899457461	44.3171492488934	4.9415746935261895
10	25.153967553895285	17.470468518351232	48.22970524313323	9.145858684620249
11	19.555872022275476	16.04477413663923	52.32479050316437	12.07456333792092
12	18.018587696411586	13.964950129922576	54.86638432639527	13.150077847270564
13	16.310464479858013	14.82366131920569	56.198821397637474	12.667052803298812
14	13.403812125044503	17.51430742498233	54.825202323196365	14.256678126776803
15	12.679274559086876	16.461642285149505	58.104086848859396	12.754996306904228
16	15.125751239445451	15.968255317792218	55.980689625854865	12.925303816907471
17	14.812768015133724	16.136968685736147	54.51115633751176	14.539106961618373
18	14.867234535493573	16.535769890907545	55.15678387153341	13.440211702065477
19	15.733650744730031	17.141809563789597	53.699472338978374	13.425067352502007
20	16.663301255652563	17.14712337065397	54.175058053339995	12.014517320353473
21	16.260514695332883	17.036330497531736	54.3575873191313	12.34556748800408
22	14.689753386223426	15.939295070381371	54.520986880210856	14.84996466318435
23	14.781416554633905	16.47545818299688	54.92085084675512	13.82227441561409
24	15.125751239445451	15.691140289815026	54.949014023136314	14.234094447603207
25	14.663450042244763	16.85911503860481	53.93832795753205	14.539106961618373
26	14.006663513807926	17.542470601363522	53.89634888330349	14.554517001525063
27	14.421671829915669	17.190962277285067	54.61371280999421	13.773653082805051
28	12.85994399247565	17.76219651520546	54.54463332075732	14.833226171561567
29	15.024257528335875	17.12294554942106	53.38276944986158	14.470027472381489
30	15.488949938625533	18.416326140210106	52.22914197960561	13.865581941558752
31	15.842052404763296	19.87682595688377	49.82251885072985	14.458602787623082
32	17.717029156858267	20.41989701842297	47.81974504354665	14.043328781172121
33	16.33543937212058	22.87168750564592	45.164170063074884	15.62870305915861
34	16.393359866942276	24.150720817901153	43.24907406915388	16.206845246002686
35	17.917891056331666	24.412957186658094	40.782936303397115	16.886215453613122
36	17.758476850400395	27.487791528729094	38.57903490639729	16.174696714473217
37	17.920547959763855	27.544383571834697	38.59949306282514	15.935575405576309
38	18.310315693265814	26.732965263644527	37.67781326219917	17.27890578089049
39	19.209411814718184	25.73662647657408	37.56383210495831	17.49012960374942
40	19.07204990727407	25.390431959360004	38.7998235816121	16.73769455175382
41	17.775481032366397	26.198396293088333	37.32524217674784	18.700880497797424
42	18.59513574119635	25.57216415412165	37.34968568832397	18.483014416358024
43	18.23246842270271	25.355892214741566	37.343840500773155	19.06779886178257
44	17.943397329280668	25.353766691995812	37.47057479448852	19.232261184234996
45	18.493376339743556	24.27851787298939	37.4703091041453	19.757796683121757
46	18.250269675698366	25.517431943418583	37.9822943955279	18.25000398535515
47	17.178740521497005	24.562009469203833	40.00366652673642	18.25558348256274
48	17.01985769625217	23.5715158696842	40.50289868164452	18.90572775241911
49	17.619255110553752	22.923497122573583	40.966528330561296	18.490719436311366
50	16.516905876539013	22.684907194363113	40.89718315098119	19.90100377811668
51	16.23314858998135	22.574911392270536	39.973112137266256	21.218827880481854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	227.5
2	346.0
3	22567.0
4	44788.0
5	29879.5
6	14971.0
7	14226.5
8	13482.0
9	13068.0
10	12654.0
11	12106.0
12	11558.0
13	10934.0
14	10310.0
15	9514.0
16	8718.0
17	8223.5
18	7729.0
19	6936.5
20	6144.0
21	5553.0
22	4962.0
23	4483.0
24	4004.0
25	3774.5
26	3448.5
27	3352.0
28	3349.5
29	3347.0
30	3515.5
31	3684.0
32	3972.5
33	4261.0
34	4991.5
35	5722.0
36	6262.5
37	6803.0
38	7383.5
39	7964.0
40	9119.0
41	10274.0
42	11465.0
43	12656.0
44	14372.5
45	16089.0
46	18996.5
47	21904.0
48	23050.5
49	24197.0
50	23961.0
51	23725.0
52	21027.0
53	18329.0
54	16416.0
55	14503.0
56	13444.0
57	12385.0
58	11851.5
59	11318.0
60	10605.0
61	9892.0
62	8847.0
63	7802.0
64	6653.0
65	5504.0
66	4444.5
67	3385.0
68	2836.0
69	2287.0
70	1897.0
71	1507.0
72	1240.5
73	974.0
74	809.0
75	474.5
76	305.0
77	211.5
78	118.0
79	98.5
80	79.0
81	54.5
82	30.0
83	21.5
84	13.0
85	8.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	376378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.73005463132008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.80110469333806	40.182433506707156
2	7.373066071055964	7.333259570278912
3	3.0266005237304516	4.515390281768919
4	1.769999845187908	3.52088755994491
5	1.305143439576156	3.2452427275915623
6	1.0625134554937776	3.170331131293094
7	0.8509404839724696	2.9622121729166997
8	0.6994069783655216	2.782523779491511
9	0.5868582987995817	2.626604573413209
>10	2.418198893506103	17.390487241038336
>50	0.07922129743745385	2.820756884781557
>100	0.02425141758289401	2.400524410165168
>500	0.002155681562923912	0.5823757444779402
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.38920390730978E-4	6.466970416131016
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24130	6.411107981869291	No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	627	0.16658784519817843	RNA PCR Primer, Index 40 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	526	0.1397531205330811	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	519	0.1378932881305496	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC	501	0.1331108619526115	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	466	0.12381169993995397	No Hit
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	436	0.11584098964339042	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	418	0.11105856346545229	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG	413	0.10973011174935836	No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	409	0.10866735037648322	Illumina PCR Primer Index 8 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.313806864375708E-4	0.0	0.0	0.11690375101626556	0.0
2	5.313806864375708E-4	0.0	0.0	0.5135794334419121	0.0
3	5.313806864375708E-4	0.0	0.0	0.7497781485634123	0.0
4	5.313806864375708E-4	0.0	0.0	1.090393168569895	0.0
5	5.313806864375708E-4	0.0	0.0	2.0976252597123106	0.0
6	5.313806864375708E-4	0.0	0.0	2.646541508802321	0.0
7	5.313806864375708E-4	0.0	0.0	3.1340832886087924	0.0
8	5.313806864375708E-4	0.0	0.0	3.883595746828986	0.0
9	5.313806864375708E-4	0.0	0.0	4.150083161077427	0.0
10	5.313806864375708E-4	0.0	0.0	4.861601900217335	0.0
11	5.313806864375708E-4	0.0	0.0	5.7747796098603	0.0
12	5.313806864375708E-4	0.0	0.0	6.6268485405629445	0.0
13	5.313806864375708E-4	0.0	0.0	6.951256449633082	0.0
14	5.313806864375708E-4	0.0	0.0	7.075333839916254	0.0
15	5.313806864375708E-4	0.0	0.0	7.324285691512256	0.0
16	5.313806864375708E-4	0.0	0.0	7.769317016403722	0.0
17	5.313806864375708E-4	0.0	0.0	8.344270919129173	0.0
18	5.313806864375708E-4	0.0	0.0	8.91151980190128	0.0
19	5.313806864375708E-4	0.0	0.0	9.312446529818427	0.0
20	5.313806864375708E-4	0.0	0.0	9.705668237782229	0.0
21	5.313806864375708E-4	0.0	0.0	10.13422676139413	0.0
22	5.313806864375708E-4	0.0	0.0	10.616189043993007	0.0
23	5.313806864375708E-4	0.0	0.0	11.07264505364288	0.0
24	5.313806864375708E-4	0.0	0.0	11.439563417628023	0.0
25	5.313806864375708E-4	0.0	0.0	11.77672446317266	0.0
26	5.313806864375708E-4	0.0	0.0	12.067655388997231	0.0
27	5.313806864375708E-4	0.0	0.0	12.358054934135364	0.0
28	5.313806864375708E-4	0.0	0.0	12.678211797714	0.0
29	5.313806864375708E-4	0.0	0.0	13.006605061932419	0.0
30	5.313806864375708E-4	0.0	0.0	13.408594551222441	0.0
31	5.313806864375708E-4	0.0	0.0	13.763556849762738	0.0
32	5.313806864375708E-4	0.0	0.0	14.09141873329472	0.0
33	5.313806864375708E-4	0.0	0.0	14.433627895360516	0.0
34	5.313806864375708E-4	0.0	0.0	14.784339148409313	0.0
35	5.313806864375708E-4	0.0	0.0	15.127079691161544	0.0
36	5.313806864375708E-4	0.0	0.0	15.442188438219025	0.0
37	7.970710296563561E-4	0.0	0.0	15.755703043217192	0.0
38	7.970710296563561E-4	0.0	0.0	16.086221830181362	0.0
39	7.970710296563561E-4	0.0	0.0	16.428696682590374	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATACG	20	7.0272345E-4	45.0	9
AGACGTG	20	7.0272345E-4	45.0	24
GTTACGG	25	3.8857892E-5	45.0	2
GTTAACG	20	7.0272345E-4	45.0	1
CGAGGGC	45	3.8380676E-10	45.0	4
GTATACG	20	7.0272345E-4	45.0	1
ATACGAG	20	7.0272345E-4	45.0	1
TATACGG	35	1.2090095E-7	45.0	2
AGTACTG	20	7.0272345E-4	45.0	39
CGTTTTT	14505	0.0	44.581177	1
TACGGCT	50	1.0768417E-9	40.5	7
GTTTTTT	16050	0.0	40.35981	2
CACGGGC	45	1.922308E-8	40.0	4
CGGGCTC	45	1.922308E-8	40.0	6
TCGATGG	35	6.237098E-6	38.571426	2
TTAACGG	35	6.237098E-6	38.571426	2
TACACGC	90	0.0	37.5	35
TAGTAGG	90	0.0	37.5	2
CGAATAT	30	1.13846385E-4	37.499996	14
GCTACGA	30	1.13846385E-4	37.499996	10
>>END_MODULE