##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935690.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541436
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42807275467461	33.0	31.0	34.0	31.0	34.0
2	32.625043403098424	34.0	31.0	34.0	31.0	34.0
3	32.47643673490496	34.0	31.0	34.0	31.0	34.0
4	36.084449500956715	37.0	35.0	37.0	35.0	37.0
5	36.12515421951994	37.0	35.0	37.0	35.0	37.0
6	36.1444695217902	37.0	36.0	37.0	35.0	37.0
7	36.29757903057794	37.0	37.0	37.0	35.0	37.0
8	36.10723151028007	37.0	37.0	37.0	35.0	37.0
9	37.92885216350594	39.0	39.0	39.0	35.0	39.0
10	37.71155039561462	39.0	38.0	39.0	35.0	39.0
11	37.693847472277426	39.0	38.0	39.0	35.0	39.0
12	37.68560642439734	39.0	38.0	39.0	35.0	39.0
13	37.72063180135787	39.0	38.0	39.0	35.0	39.0
14	39.157737941326396	41.0	39.0	41.0	35.0	41.0
15	39.24517025096225	41.0	39.0	41.0	35.0	41.0
16	39.28914774784093	41.0	39.0	41.0	35.0	41.0
17	39.24405469898566	41.0	39.0	41.0	35.0	41.0
18	38.59438973396671	39.0	39.0	41.0	35.0	41.0
19	37.90990994318811	37.0	37.0	41.0	35.0	41.0
20	37.11097895226767	37.0	35.0	41.0	34.0	41.0
21	37.08723838089821	37.0	35.0	41.0	34.0	41.0
22	37.08119519204486	37.0	35.0	41.0	34.0	41.0
23	37.08307537732991	37.0	35.0	41.0	34.0	41.0
24	36.9403105814907	37.0	35.0	41.0	34.0	41.0
25	36.86374566892486	37.0	35.0	40.0	34.0	41.0
26	36.7163118078591	36.0	35.0	40.0	34.0	41.0
27	36.64241203023072	36.0	35.0	40.0	33.0	41.0
28	36.503160114953566	36.0	35.0	40.0	33.0	41.0
29	36.23548120184103	36.0	35.0	40.0	33.0	41.0
30	35.68678292540577	36.0	35.0	40.0	31.0	41.0
31	34.979901595017694	36.0	35.0	40.0	24.0	41.0
32	33.83919613767832	36.0	34.0	40.0	17.0	41.0
33	32.574372594360185	36.0	33.0	40.0	12.0	41.0
34	31.55647758922569	36.0	27.0	40.0	7.0	41.0
35	30.92455618023183	36.0	23.0	40.0	7.0	41.0
36	30.56911804903996	36.0	22.0	40.0	7.0	41.0
37	30.425483344291845	36.0	21.0	40.0	7.0	41.0
38	30.260004875922547	35.0	21.0	40.0	7.0	41.0
39	30.15217311002593	35.0	20.0	40.0	7.0	41.0
40	30.052731994178444	35.0	20.0	40.0	7.0	41.0
41	29.897733065403852	35.0	18.0	40.0	7.0	41.0
42	29.826563065625486	35.0	18.0	40.0	7.0	41.0
43	29.74046055304782	35.0	18.0	40.0	7.0	41.0
44	29.62616264895574	35.0	18.0	40.0	7.0	41.0
45	29.554795026559002	35.0	18.0	40.0	7.0	41.0
46	29.46055304782098	35.0	18.0	40.0	7.0	41.0
47	29.445753514727503	35.0	18.0	40.0	7.0	41.0
48	29.38855561876196	35.0	18.0	40.0	7.0	41.0
49	29.349919473400366	35.0	18.0	40.0	7.0	41.0
50	29.2224953641797	35.0	18.0	40.0	7.0	41.0
51	28.54949984855089	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	7.0
11	7.0
12	10.0
13	6.0
14	19.0
15	26.0
16	56.0
17	101.0
18	247.0
19	495.0
20	820.0
21	1398.0
22	2371.0
23	4271.0
24	8842.0
25	18684.0
26	31030.0
27	35565.0
28	31113.0
29	22959.0
30	17156.0
31	14119.0
32	13061.0
33	14025.0
34	21368.0
35	29954.0
36	31330.0
37	35042.0
38	70554.0
39	136656.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.764197430536573	13.650551496391078	15.649125658434237	43.93612541463811
2	58.0116578875435	15.321663132854113	15.884610554155984	10.782068425446406
3	20.490325726401643	15.474774488582216	53.39338352085935	10.641516264156797
4	18.22449929446878	16.32492113564669	53.13776697522884	12.312812594655693
5	16.310699694885454	19.21095013999808	52.003376206975524	12.474973958140943
6	16.643148959433802	22.041016851483832	51.63287997103998	9.682954218042392
7	51.91693939819295	3.496812180941053	41.42354775079603	3.1627006700699622
8	52.740859492165285	3.148663923344587	40.97880451244468	3.1316720720454496
9	48.740756063505195	4.88441847235869	42.26538316624679	4.109442297889316
10	24.141911509393537	19.568702487459273	47.270776232093915	9.018609771053274
11	17.765349921320343	16.638900996609017	53.121698594109	12.474050487961643
12	16.473599834514143	14.750404479938533	55.762638612873914	13.01335707267341
13	15.314275371419706	15.526119430551349	56.71935371862972	12.440251479399226
14	13.052327514239911	18.414180069297203	54.887004188860736	13.646488227602156
15	12.312443206583973	18.290065677199152	57.17000716612859	12.227483950088283
16	14.483706292156414	17.352189363101086	55.672507923374134	12.491596421368362
17	14.332811264858636	17.33704445216055	54.150444373850284	14.179699909130534
18	15.082484356415163	17.23047599346922	54.74866835600145	12.93837129411417
19	14.979979166512756	18.58299041807342	53.21700071661286	13.220029698800968
20	15.641922591035689	18.01173176515784	54.14398008259518	12.202365561211298
21	15.187759956855473	17.651024313122882	54.837875575321924	12.323340154699725
22	14.391543968262177	16.886021616589957	54.193847472277426	14.528586942870442
23	13.558389172496843	17.654533499804224	54.78394491685074	14.00313241084819
24	14.421095013999807	16.779637851934485	54.63655907623431	14.162708057831397
25	13.554325903707918	18.171122718105188	53.56311733981486	14.711434038372031
26	13.505197290169106	18.62122208349648	53.978124838392716	13.895455787941696
27	14.42238787225083	17.872472462119255	53.79934101168005	13.905798653949866
28	12.748690519285752	18.22228296603846	54.16134132196603	14.867685192709757
29	13.768016903198163	17.366410803862323	54.417327255668255	14.448245037271256
30	15.4038519788119	17.279419912972173	53.07552508514395	14.24120302307198
31	15.31907741635207	18.65853027874024	50.64273524479348	15.379657060114216
32	15.905850368279909	19.807511875826506	49.73034670764412	14.55629104824947
33	16.23829963282826	20.70475550203533	47.404494714056696	15.652450151079723
34	16.40433957106657	21.964184132565993	44.952496693976755	16.67897960239068
35	17.200001477552288	23.068100384902372	42.92954291920005	16.802355218345287
36	17.262058673601313	24.950317304353607	41.424471220975335	16.363152801069745
37	18.253865646170553	24.946069341528823	40.56084190929307	16.239223103007557
38	17.63015388707068	25.033429620490693	39.93269749333255	17.40371899910608
39	18.816997761508286	23.762549959736702	40.44743977127491	16.97301250748011
40	18.733331363263616	23.302107728337237	41.42484060904705	16.539720299352094
41	17.348126094312164	24.24035343050702	40.39092339630169	18.020597078879128
42	17.825375482974902	24.46752709461506	40.221189577346166	17.485907845063867
43	18.425077017412953	23.956294003354046	39.491832829734264	18.12679614949874
44	17.85123264799533	24.226316683781647	38.75619648490311	19.16625418331991
45	18.340117760917266	22.994038076522433	38.90967722870293	19.756166933857372
46	18.428770898130157	23.391869029765292	39.91921482871475	18.2601452433898
47	17.28052807718733	23.05812690696592	41.69412451333122	17.96722050251553
48	17.21126781373976	22.41132839338352	41.43038143012286	18.947022362753863
49	17.471317016230913	21.88735141364815	42.01253703115419	18.628794538966748
50	16.650721414904073	21.62065322586603	41.788687859691635	19.939937499538267
51	16.682119401000303	21.402714263551	40.475882652797374	21.43928368265132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	252.5
2	385.0
3	27798.5
4	55212.0
5	37814.0
6	20416.0
7	19741.0
8	19066.0
9	18708.0
10	18350.0
11	17705.0
12	17060.0
13	16041.5
14	15023.0
15	13952.0
16	12881.0
17	11812.0
18	10743.0
19	9918.5
20	9094.0
21	8197.0
22	7300.0
23	6732.0
24	6164.0
25	5930.0
26	5801.5
27	5907.0
28	6235.5
29	6564.0
30	7188.0
31	7812.0
32	8360.5
33	8909.0
34	10021.5
35	11134.0
36	12143.5
37	13153.0
38	14028.5
39	14904.0
40	16156.0
41	17408.0
42	19199.0
43	20990.0
44	22896.0
45	24802.0
46	28843.5
47	32885.0
48	34695.5
49	36506.0
50	35905.0
51	35304.0
52	30901.5
53	26499.0
54	23028.0
55	19557.0
56	17552.0
57	15547.0
58	14212.0
59	12877.0
60	12060.0
61	11243.0
62	10020.5
63	8798.0
64	7338.5
65	5879.0
66	4431.5
67	2984.0
68	2332.0
69	1680.0
70	1447.5
71	1215.0
72	922.0
73	629.0
74	481.0
75	281.0
76	229.0
77	172.0
78	115.0
79	67.5
80	20.0
81	20.5
82	21.0
83	17.0
84	13.0
85	9.5
86	6.0
87	4.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	541436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.30017732232487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62182986205329	38.9404867838748
2	7.730864536495181	7.468042559351802
3	2.910743024390073	4.2176821265328215
4	1.7026995039723043	3.289627518739876
5	1.172405756187108	2.8313702958775853
6	0.8913869392265562	2.583248833646825
7	0.7434920805231641	2.513755952890915
8	0.6255273073574343	2.417046389225639
9	0.5172399728188575	2.248450416481078
>10	2.9691765102260623	21.583609659170648
>50	0.08193674477447858	2.8434970677903957
>100	0.029235646001639144	2.4536867653517107
>500	0.0030774364212251726	1.0148445488680178
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.846795526531466E-4	5.594651082197893
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30111	5.561322113786302	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	932	0.1721348414217008	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	918	0.1695491249196581	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	679	0.1254072503490717	No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	631	0.11654193662778242	TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	629	0.11617254855606203	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	578	0.10675315272719213	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	573	0.10582968254789116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.69388071720388E-4	0.0	0.0	0.12171336963186785	0.0
2	3.69388071720388E-4	0.0	0.0	0.5875117280712772	0.0
3	3.69388071720388E-4	0.0	0.0	0.9050007757149506	0.0
4	3.69388071720388E-4	0.0	0.0	1.2577663842079212	0.0
5	3.69388071720388E-4	0.0	0.0	2.233135587585606	0.0
6	3.69388071720388E-4	0.0	0.0	2.809565673505271	0.0
7	3.69388071720388E-4	0.0	0.0	3.3189518244076863	0.0
8	3.69388071720388E-4	0.0	0.0	4.091342282375018	0.0
9	3.69388071720388E-4	0.0	0.0	4.351760872937891	0.0
10	3.69388071720388E-4	0.0	0.0	5.065603321537541	0.0
11	3.69388071720388E-4	0.0	0.0	6.029336800656033	0.0
12	3.69388071720388E-4	0.0	0.0	6.82610687135691	0.0
13	3.69388071720388E-4	0.0	0.0	7.165020427160366	0.0
14	3.69388071720388E-4	0.0	0.0	7.297261356836265	0.0
15	3.69388071720388E-4	0.0	0.0	7.5284982897332275	0.0
16	3.69388071720388E-4	0.0	0.0	8.038069134671503	0.0
17	3.69388071720388E-4	0.0	0.0	8.69096255143729	0.0
18	3.69388071720388E-4	0.0	0.0	9.352351893852644	0.0
19	3.69388071720388E-4	0.0	0.0	9.78656757215996	0.0
20	5.54082107580582E-4	0.0	0.0	10.194556697375129	0.0
21	5.54082107580582E-4	0.0	0.0	10.713916326213994	0.0
22	5.54082107580582E-4	0.0	0.0	11.28074232226893	0.0
23	5.54082107580582E-4	0.0	0.0	11.856433632045155	0.0
24	5.54082107580582E-4	0.0	0.0	12.283630936989782	0.0
25	5.54082107580582E-4	0.0	0.0	12.677768009515436	0.0
26	5.54082107580582E-4	0.0	0.0	13.039398931729696	0.0
27	5.54082107580582E-4	0.0	0.0	13.389024741613044	0.0
28	5.54082107580582E-4	0.0	0.0	13.776328134811871	0.0
29	5.54082107580582E-4	0.0	0.0	14.179515215094675	0.0
30	5.54082107580582E-4	0.0	0.0	14.6419890808886	0.0
31	7.38776143440776E-4	0.0	0.0	15.058104743681618	0.0
32	7.38776143440776E-4	0.0	0.0	15.453904062530013	0.0
33	7.38776143440776E-4	0.0	0.0	15.854320732274914	0.0
34	7.38776143440776E-4	0.0	0.0	16.23349758789589	0.0
35	7.38776143440776E-4	0.0	0.0	16.61101219719413	0.0
36	7.38776143440776E-4	0.0	0.0	17.006442127970804	0.0
37	7.38776143440776E-4	0.0	0.0	17.388758782201407	0.0
38	7.38776143440776E-4	0.0	0.0	17.789729534053887	0.0
39	7.38776143440776E-4	0.0	0.0	18.181465584113358	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	30	2.163366E-6	45.000004	2
TTGCGAG	20	7.029975E-4	45.000004	1
TGTGTCG	20	7.029975E-4	45.000004	1
CTGGCGA	20	7.029975E-4	45.000004	14
CGCGGGT	25	3.888063E-5	45.0	4
TAGTTCG	35	1.2103374E-7	45.0	1
CGTTTTT	19970	0.0	44.63946	1
ACGGGCG	55	6.002665E-11	40.90909	5
TAGGGCG	110	0.0	40.90909	5
GTTTTTT	22235	0.0	40.102318	2
CGGGTGA	45	1.9250365E-8	40.0	6
TCGTTGG	40	3.453697E-7	39.375004	2
TACGGGT	40	3.453697E-7	39.375004	4
AGACACG	40	3.453697E-7	39.375004	24
GCGAAAG	35	6.2421695E-6	38.571426	1
TACTAGG	35	6.2421695E-6	38.571426	2
TTACGGG	65	9.094947E-12	38.07692	3
CCCGCAC	125	0.0	37.8	33
CGGGTCA	30	1.1391243E-4	37.500004	6
TGCGTAG	60	1.546141E-10	37.500004	1
>>END_MODULE