##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935689.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284062
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.307996141687376	33.0	31.0	34.0	30.0	34.0
2	32.50872344769804	34.0	31.0	34.0	30.0	34.0
3	32.37541452218178	34.0	31.0	34.0	30.0	34.0
4	36.03409466947357	37.0	35.0	37.0	35.0	37.0
5	36.021498827720706	37.0	35.0	37.0	35.0	37.0
6	36.03254571185164	37.0	35.0	37.0	35.0	37.0
7	36.16366497454781	37.0	36.0	37.0	35.0	37.0
8	35.70990135956235	37.0	35.0	37.0	35.0	37.0
9	37.60276981785667	39.0	38.0	39.0	35.0	39.0
10	37.557568418162234	39.0	37.0	39.0	35.0	39.0
11	37.549446951721805	39.0	37.0	39.0	35.0	39.0
12	37.4509649301913	39.0	37.0	39.0	35.0	39.0
13	37.36981011187699	39.0	37.0	39.0	34.0	39.0
14	38.778611711527766	40.0	38.0	41.0	35.0	41.0
15	38.81028085417972	41.0	38.0	41.0	35.0	41.0
16	38.954393759108925	41.0	38.0	41.0	35.0	41.0
17	38.803451359210314	41.0	38.0	41.0	34.0	41.0
18	38.21213678703945	39.0	38.0	40.0	35.0	41.0
19	37.558469629869535	37.0	37.0	40.0	35.0	41.0
20	36.55164365525836	35.0	35.0	40.0	33.0	41.0
21	36.6743070174821	35.0	35.0	40.0	33.0	41.0
22	36.620061817490544	35.0	35.0	40.0	33.0	41.0
23	36.5085192669206	35.0	35.0	40.0	33.0	41.0
24	36.42750174257733	35.0	35.0	40.0	33.0	41.0
25	36.47207299814829	35.0	35.0	40.0	33.0	41.0
26	36.24302793052221	35.0	35.0	40.0	33.0	41.0
27	36.05624476346713	35.0	35.0	40.0	33.0	41.0
28	35.924762903873095	35.0	35.0	40.0	32.0	41.0
29	35.73256894621597	35.0	35.0	40.0	31.0	41.0
30	35.22586970450113	35.0	35.0	40.0	30.0	41.0
31	34.60699072737642	35.0	35.0	40.0	23.0	41.0
32	33.4054783814801	35.0	33.0	40.0	16.0	41.0
33	32.30728502932458	35.0	31.0	40.0	12.0	41.0
34	31.285127894614558	35.0	27.0	40.0	8.0	41.0
35	30.752110454759876	35.0	23.0	40.0	7.0	41.0
36	30.343048348599954	35.0	22.0	40.0	7.0	41.0
37	30.098594672993922	35.0	21.0	40.0	7.0	41.0
38	30.048334518520605	35.0	21.0	40.0	7.0	41.0
39	29.76312565566672	35.0	19.0	40.0	7.0	41.0
40	29.700780111384134	35.0	19.0	40.0	7.0	41.0
41	29.597489280509183	35.0	20.0	40.0	7.0	41.0
42	29.397758235878083	35.0	18.0	40.0	7.0	41.0
43	29.271944153037012	35.0	18.0	40.0	7.0	41.0
44	29.27255317501109	35.0	18.0	40.0	7.0	41.0
45	29.2593975962994	35.0	18.0	40.0	7.0	41.0
46	29.23379403088058	35.0	18.0	40.0	7.0	41.0
47	29.149844048130337	35.0	18.0	40.0	7.0	41.0
48	29.19217283550774	35.0	20.0	40.0	7.0	41.0
49	29.182879089776176	35.0	20.0	40.0	7.0	41.0
50	29.02203392217192	35.0	18.0	40.0	7.0	41.0
51	28.508987474565412	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	4.0
11	5.0
12	8.0
13	6.0
14	5.0
15	16.0
16	29.0
17	71.0
18	170.0
19	339.0
20	601.0
21	1173.0
22	2059.0
23	3392.0
24	5228.0
25	9409.0
26	14932.0
27	18467.0
28	16028.0
29	12507.0
30	9712.0
31	8439.0
32	8032.0
33	8840.0
34	13046.0
35	19491.0
36	18754.0
37	18080.0
38	32163.0
39	62979.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.28907773655047	11.438700002112215	12.633509585935466	43.63871267540185
2	54.755299899317755	21.949433574360526	13.187966007420915	10.107300518900804
3	18.125268427315163	22.349698305299547	50.00140814329266	9.523625124092627
4	16.937851595778387	13.942730812287458	58.58122522547895	10.538192366455212
5	21.52839872985475	16.541459258894182	49.71837134146771	12.211770669783357
6	14.765086495201752	26.264688694721578	50.23199160746598	8.738233202610697
7	44.60716322492977	3.76291091381459	48.241229027465835	3.388696833789807
8	43.47853637586161	10.455815983834515	42.095387626644886	3.9702600136589896
9	40.2250212981673	5.080228964099387	42.42770944371299	12.267040294020319
10	27.811182065887024	15.130499679647402	47.31678295583359	9.74153529863199
11	23.379051052235074	14.149727876308694	50.53368630791869	11.937534763537537
12	18.16293626039386	13.026381564588013	52.54064253578444	16.270039639233687
13	17.591934155219636	14.73481141440953	55.900120396251516	11.773134034119312
14	12.24803035956939	22.033922171920214	51.51410607543424	14.203941393076159
15	10.759622899226224	15.325175489857848	58.86813442135872	15.04706718955721
16	12.000197140060973	16.070787363322093	54.01813688560948	17.910878611007455
17	11.937534763537537	17.990790742865993	54.82007449078018	15.251600002816287
18	12.236765213228098	16.9163774105653	53.81606832311257	17.030789053094043
19	14.579211580570439	16.41648654167048	51.97703318289669	17.02726869486239
20	18.103794242102076	16.26088670783139	54.6532095105998	10.982109539466736
21	14.828452943371516	20.473699403651317	52.450169329230945	12.247678323746225
22	12.802134745231674	15.471974428117804	55.10733572248312	16.618555104167402
23	15.46246946089234	18.169976976857168	52.82579155254838	13.541762009702108
24	14.612654983771147	17.082186283276187	52.23753969203906	16.067619040913602
25	12.725390935781626	21.459047672691174	51.47573417070921	14.339827220817991
26	12.570847209412031	16.09824615752899	55.8078870105822	15.523019622476783
27	16.974815357210748	17.092747357971145	52.120663798748154	13.811773486069942
28	11.093704895410157	18.782167273341734	54.972506002210785	15.151621829037323
29	14.348980152220289	15.17696840830523	55.955037984665324	14.519013454809162
30	15.152325900683653	19.4858868838493	51.98090557695151	13.380881638515534
31	14.973843738338813	16.250677668959593	49.08435482394688	19.69112376875471
32	18.149910934936738	19.324654476839566	49.213551971048574	13.311882617175124
33	18.677260598038455	19.219747801536286	43.911540438355004	18.19145116207025
34	15.42304144869782	20.33288507438517	46.13746294822961	18.1066105286874
35	19.10216783659905	20.531433278650436	42.56042694904634	17.805971935704175
36	16.335870338165613	24.504509578894748	40.00570298033528	19.15391710260436
37	18.01860157289606	23.29280227555956	42.270349430758074	16.418246720786307
38	17.481042870922543	23.493814730586983	37.645302786011506	21.379839612478964
39	17.739789200949087	23.281185093395106	41.50396744372708	17.475058261928734
40	18.574818173497334	21.90859741887334	41.576838859122304	17.939745548507016
41	15.212171990621764	24.17324386929614	38.87637205962079	21.73821208046131
42	16.656222937246092	23.154100161232407	41.60570579662187	18.583971104899636
43	18.187578768015435	23.450514324337647	37.18519196513437	21.17671494251255
44	18.232639353380602	22.526772324351725	40.26726559694714	18.97332272532053
45	16.225331089691686	21.30203969555942	39.955009821799464	22.517619392949424
46	19.475677844977504	23.94688483500081	39.13511838964733	17.442318930374352
47	15.77402116439369	22.49684927938267	44.71946265251952	17.009666903704122
48	16.71747717047687	22.016672416585113	40.73371306264125	20.532137350296768
49	15.531116446409587	19.92874794939133	45.65834219290155	18.881793411297533
50	16.38093092353078	20.537065851821083	42.41679633319487	20.665206891453273
51	15.387837866381282	20.405756489780398	41.26986362132211	22.936542022516214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	150.5
2	228.0
3	16736.0
4	33244.0
5	22208.5
6	11173.0
7	10593.0
8	10013.0
9	9533.5
10	9054.0
11	8717.5
12	8381.0
13	7932.5
14	7484.0
15	6810.0
16	6136.0
17	5570.0
18	5004.0
19	4543.5
20	4083.0
21	3731.0
22	3379.0
23	3015.5
24	2652.0
25	2589.5
26	2530.5
27	2534.0
28	2692.5
29	2851.0
30	2904.0
31	2957.0
32	3325.5
33	3694.0
34	4035.0
35	4376.0
36	4593.5
37	4811.0
38	5261.5
39	5712.0
40	6557.0
41	7402.0
42	8202.5
43	9003.0
44	10189.5
45	11376.0
46	19473.5
47	27571.0
48	22875.5
49	18180.0
50	17586.5
51	16993.0
52	14843.5
53	12694.0
54	11825.5
55	10957.0
56	10157.0
57	9357.0
58	8573.0
59	7789.0
60	7366.5
61	6944.0
62	6240.0
63	5536.0
64	4660.5
65	3785.0
66	3008.0
67	2231.0
68	1926.5
69	1622.0
70	1307.5
71	993.0
72	772.5
73	552.0
74	475.0
75	277.5
76	157.0
77	119.5
78	82.0
79	60.5
80	39.0
81	26.5
82	14.0
83	16.0
84	18.0
85	10.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	284062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.212110980062576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63317717270426	29.66527581858236
2	8.538934454977532	6.525814220901633
3	3.0496438705636106	3.4959999009493288
4	1.6903965006022494	2.583744747252903
5	1.19873558192052	2.29031085460484
6	0.9553214631806636	2.190290986361717
7	0.8686030241705558	2.323380861005628
8	0.64148330521699	1.9609945000647184
9	0.6564230204224336	2.2574978375624926
>10	4.626833070464637	28.115745331773923
>50	0.09462844609057076	2.4755953953663123
>100	0.03585920062379524	2.6145241037471094
>500	0.004980444531082672	1.3321930940624374
>1k	0.003984355624866137	4.829770905873791
>5k	0.0	0.0
>10k+	9.960889062165343E-4	7.338861441890816
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19281	6.787602706451408	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	4157	1.4634129168984236	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	3740	1.3166139786384663	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	3484	1.2264928079081328	TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1308	0.4604628567002978	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT	985	0.34675528581788484	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	840	0.2957100914589068	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	580	0.20418077743591187	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA	568	0.1999563475579275	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	527	0.1855228788081475	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	481	0.1693292309425407	Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	431	0.15172743978427244	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	353	0.12426864557737395	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	343	0.1207482873457203	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	340	0.1196921798762242	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	294	0.1034985320106174	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.520358231653653E-4	0.0	0.0	0.12497271722370469	0.0
2	3.520358231653653E-4	0.0	0.0	0.9293745731565645	0.0
3	3.520358231653653E-4	0.0	0.0	1.208538980926699	0.0
4	3.520358231653653E-4	0.0	0.0	1.6489357957065711	0.0
5	3.520358231653653E-4	0.0	0.0	3.63300969506657	0.0
6	3.520358231653653E-4	0.0	0.0	4.088544050242553	0.0
7	3.520358231653653E-4	0.0	0.0	4.778182227823503	0.0
8	3.520358231653653E-4	0.0	0.0	5.522033922171921	0.0
9	3.520358231653653E-4	0.0	0.0	5.725862663784667	0.0
10	3.520358231653653E-4	0.0	0.0	7.595172884792756	0.0
11	3.520358231653653E-4	0.0	0.0	8.341840865726496	0.0
12	3.520358231653653E-4	0.0	0.0	10.445254909139553	0.0
13	3.520358231653653E-4	0.0	0.0	10.771592117213848	0.0
14	3.520358231653653E-4	0.0	0.0	10.940217276510058	0.0
15	3.520358231653653E-4	0.0	0.0	11.36090008519267	0.0
16	3.520358231653653E-4	0.0	0.0	11.819250726953975	0.0
17	3.520358231653653E-4	0.0	0.0	12.298019446458872	0.0
18	3.520358231653653E-4	0.0	0.0	12.791221634713548	0.0
19	3.520358231653653E-4	0.0	0.0	13.488252564580971	0.0
20	3.520358231653653E-4	0.0	0.0	13.887813223873662	0.0
21	3.520358231653653E-4	0.0	0.0	14.273292450239737	0.0
22	3.520358231653653E-4	0.0	0.0	14.771071104195563	0.0
23	3.520358231653653E-4	0.0	0.0	15.149861649921496	0.0
24	3.520358231653653E-4	0.0	0.0	15.464229640008167	0.0
25	3.520358231653653E-4	0.0	0.0	15.749378656772112	0.0
26	3.520358231653653E-4	0.0	0.0	15.997563912103695	0.0
27	3.520358231653653E-4	0.0	0.0	16.33833458892777	0.0
28	3.520358231653653E-4	0.0	0.0	16.589336130844675	0.0
29	3.520358231653653E-4	0.0	0.0	16.85617928480402	0.0
30	3.520358231653653E-4	0.0	0.0	17.152945483732424	0.0
31	3.520358231653653E-4	0.0	0.0	17.438446536319535	0.0
32	3.520358231653653E-4	0.0	0.0	17.712682442565356	0.0
33	3.520358231653653E-4	0.0	0.0	17.99360702945132	0.0
34	3.520358231653653E-4	0.0	0.0	18.26608275658131	0.0
35	3.520358231653653E-4	0.0	0.0	18.56073674057072	0.0
36	7.040716463307306E-4	0.0	0.0	18.843069470749345	0.0
37	7.040716463307306E-4	0.0	0.0	19.131738845744945	0.0
38	7.040716463307306E-4	0.0	0.0	19.411959360984575	0.0
39	7.040716463307306E-4	0.0	0.0	19.68478712393773	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCGGC	30	2.1597116E-6	45.000004	6
AAACGAT	30	2.1597116E-6	45.000004	16
TCTCGAA	60	0.0	45.000004	42
AATTATC	60	0.0	45.000004	45
CTCTCGA	60	0.0	45.000004	41
AACAAGC	45	3.8198777E-10	45.0	13
CTACTGG	25	3.8833678E-5	45.0	2
ACTGACG	20	7.024311E-4	45.0	43
GCGAACC	20	7.024311E-4	45.0	33
CTATATC	20	7.024311E-4	45.0	19
CGTCTAC	20	7.024311E-4	45.0	42
AATGGGC	35	1.2076089E-7	45.0	4
AGGGTGT	20	7.024311E-4	45.0	6
CCTCGAA	40	6.7830115E-9	45.0	42
CCGTCTA	25	3.8833678E-5	45.0	41
CACCTCG	25	3.8833678E-5	45.0	38
GTTACGG	20	7.024311E-4	45.0	2
TACGCGG	20	7.024311E-4	45.0	2
TAACCAG	20	7.024311E-4	45.0	1
TGCGAAG	20	7.024311E-4	45.0	1
>>END_MODULE