##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935688.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561361
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41768487657675	34.0	31.0	34.0	30.0	34.0
2	32.620499108416865	34.0	31.0	34.0	31.0	34.0
3	32.46855766610078	34.0	31.0	34.0	31.0	34.0
4	36.09041597118432	37.0	35.0	37.0	35.0	37.0
5	36.10108290387113	37.0	35.0	37.0	35.0	37.0
6	36.101118531568815	37.0	36.0	37.0	35.0	37.0
7	36.22855346203245	37.0	37.0	37.0	35.0	37.0
8	35.90480279178639	37.0	37.0	37.0	35.0	37.0
9	37.749118659828525	39.0	38.0	39.0	35.0	39.0
10	37.68442054221793	39.0	38.0	39.0	35.0	39.0
11	37.6550989470234	39.0	37.0	39.0	35.0	39.0
12	37.53588688918539	39.0	37.0	39.0	35.0	39.0
13	37.47969488439703	39.0	37.0	39.0	35.0	39.0
14	38.814237184271796	41.0	38.0	41.0	35.0	41.0
15	38.91175909975934	41.0	39.0	41.0	35.0	41.0
16	39.01309674166891	41.0	39.0	41.0	35.0	41.0
17	38.94740282990803	41.0	38.0	41.0	35.0	41.0
18	38.28092261485924	39.0	38.0	40.0	35.0	41.0
19	37.569597816734685	37.0	37.0	40.0	35.0	41.0
20	36.63887943765242	35.0	35.0	40.0	34.0	41.0
21	36.650880627617525	35.0	35.0	40.0	33.0	41.0
22	36.62953251116483	35.0	35.0	40.0	33.0	41.0
23	36.58045713898899	35.0	35.0	40.0	33.0	41.0
24	36.442032845174495	35.0	35.0	40.0	33.0	41.0
25	36.41094055340503	35.0	35.0	40.0	33.0	41.0
26	36.22110549183146	35.0	35.0	40.0	33.0	41.0
27	36.092398652560476	35.0	35.0	40.0	33.0	41.0
28	35.93296470542129	35.0	35.0	40.0	33.0	41.0
29	35.67119375945247	35.0	35.0	40.0	32.0	41.0
30	35.12417855889526	35.0	35.0	40.0	30.0	41.0
31	34.443874797144794	35.0	35.0	40.0	23.0	41.0
32	33.25877109382376	35.0	33.0	40.0	16.0	41.0
33	32.05746035082594	35.0	31.0	40.0	10.0	41.0
34	31.014160228444798	35.0	25.0	40.0	7.0	41.0
35	30.383158074750472	35.0	22.0	40.0	7.0	41.0
36	29.991884010467416	35.0	21.0	40.0	7.0	41.0
37	29.760879362834256	35.0	20.0	40.0	7.0	41.0
38	29.66100423791464	35.0	20.0	40.0	7.0	41.0
39	29.47285436644156	35.0	18.0	40.0	7.0	41.0
40	29.341006945619664	35.0	18.0	40.0	7.0	41.0
41	29.149150012202487	35.0	18.0	40.0	7.0	41.0
42	28.97139095875916	35.0	18.0	40.0	7.0	41.0
43	28.901211519859768	35.0	18.0	40.0	7.0	41.0
44	28.86956699877619	35.0	18.0	40.0	7.0	41.0
45	28.830853942471958	35.0	18.0	40.0	7.0	41.0
46	28.83427776421946	35.0	18.0	40.0	7.0	41.0
47	28.830264304075275	35.0	18.0	40.0	7.0	41.0
48	28.82714688052786	35.0	18.0	39.0	7.0	41.0
49	28.83212941404907	35.0	18.0	39.0	7.0	41.0
50	28.708964463152945	35.0	18.0	39.0	7.0	41.0
51	28.13601229868124	34.0	18.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	16.0
10	9.0
11	9.0
12	8.0
13	15.0
14	17.0
15	20.0
16	58.0
17	156.0
18	319.0
19	663.0
20	1173.0
21	2022.0
22	3278.0
23	5589.0
24	9860.0
25	19160.0
26	31411.0
27	37945.0
28	34141.0
29	25741.0
30	19298.0
31	16309.0
32	15592.0
33	17819.0
34	28482.0
35	37196.0
36	39163.0
37	37279.0
38	64365.0
39	114145.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.56468475722396	11.577220362654336	13.311576685947188	46.54651819417451
2	59.05700609768046	17.0678048528487	13.553132476249685	10.322056573221154
3	19.594164895673195	17.303838350010064	52.8177055406414	10.284291213675335
4	17.23578944743222	14.84143002452967	56.5121196520599	11.410660875978202
5	18.544394783392505	16.91246809094326	52.37716905876967	12.165968066894566
6	15.901175892162083	22.494081348722123	52.66646596396971	8.938276795146082
7	46.286436001075955	3.8141231756392058	46.68546621514498	3.21397460813986
8	45.96364906005226	6.608403505052898	43.9093560115505	3.5185914233443363
9	42.496895936839216	4.939602145499954	44.80539260832156	7.7581093093392655
10	27.851418249575584	13.983158787304426	49.14039272411158	9.025030239008409
11	21.51111316960031	14.525234207577656	51.97867326016592	11.984979362656116
12	18.076068697326676	12.946570923167089	54.82586072064144	14.151499658864793
13	17.572293052064538	13.744809489793555	56.61615252929933	12.066744928842581
14	13.39654874492528	16.964662668051396	55.59007483597899	14.048713751044337
15	12.369224082185973	14.803308388007006	58.43245255726707	14.395014972539952
16	14.803130249518581	15.07247564401517	55.09253403781168	15.031860068654574
17	15.011908557951124	16.276513687270757	54.94806372370008	13.763514031078042
18	15.04575487075162	14.919276543970813	55.26408140216367	14.770887183113896
19	15.471149581107344	15.733903851532258	53.80940250569598	14.985544061664418
20	17.30401648849849	15.991313967304462	54.85044383204391	11.854225712153141
21	15.654988501160572	17.69574302454214	54.94752930823481	11.701739166062481
22	14.901462695128448	13.979417879047528	56.44656468831999	14.672554737504031
23	15.299424078266927	16.046715037204223	55.38877834406024	13.265082540468612
24	15.131795760660252	15.683312520819936	54.50040170229139	14.684490016228416
25	14.635501931911907	18.052198139877902	53.27498704042497	14.037312887785221
26	13.760663815263264	15.21854920452258	55.440438505703106	15.580348474511055
27	15.229950067781694	15.806228077832268	54.48383482286799	14.479987031518043
28	12.545581185725407	17.007772182249926	55.37096449521787	15.075682136806796
29	14.405703281845373	16.172480810031335	55.1566638936442	14.265152014479096
30	15.724640650134228	17.120890122398954	53.177545287257225	13.976923940209598
31	16.127055495483297	16.61818330806736	50.40464157645437	16.850119619994977
32	17.39183876329136	19.574747800435013	48.56429285254943	14.469120583724198
33	17.397895471897762	19.142405689030767	45.63783376472537	17.8218650743461
34	16.95468691269967	20.80728800183839	44.91672916358635	17.321295921875585
35	18.282174928432863	23.29623896209391	41.324388406034615	17.097197703438606
36	18.477949127210476	24.441847581146533	40.089888681258586	16.990314610384406
37	18.93576504245931	24.966109152577395	39.13488824481929	16.963237560144005
38	17.90737154878946	24.88469986336778	37.481763072247624	19.726165515595135
39	18.814452731842792	25.064085321210417	37.76500326884126	18.356458678105533
40	19.382536371425875	23.653941046848644	38.628796799207635	18.334725782517843
41	18.310855225069073	24.499030035930534	37.99195170309302	19.198163035907374
42	17.916812888675913	25.217996975208468	38.941786123368026	17.92340401274759
43	18.315843102744935	23.936646827977007	38.977413821052764	18.770096248225297
44	18.759051661943026	23.10402753308477	38.787161915416284	19.34975888955592
45	18.161931448746884	22.586535224214007	38.4328444619416	20.818688865097503
46	18.965157893049213	23.54420773797966	39.05151943223701	18.439114936734118
47	16.296999613439482	23.275574897436766	42.5998599831481	17.827565505975656
48	16.73468587949644	23.336854537454506	40.97381186081684	18.954647722232217
49	17.24291498696917	22.077771701276006	42.56957644011607	18.10973687163875
50	16.46142143825453	22.02272690835309	42.17143691848917	19.34441473490321
51	16.43274114161832	21.47904824168405	41.158007057846916	20.93020355885072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	352.5
2	556.0
3	34942.0
4	69328.0
5	45797.0
6	22266.0
7	21043.0
8	19820.0
9	19529.5
10	19239.0
11	18391.5
12	17544.0
13	16530.5
14	15517.0
15	14097.0
16	12677.0
17	11489.0
18	10301.0
19	9275.5
20	8250.0
21	7622.5
22	6995.0
23	6325.5
24	5656.0
25	5598.0
26	5432.5
27	5325.0
28	5332.5
29	5340.0
30	5618.5
31	5897.0
32	6499.0
33	7101.0
34	7888.0
35	8675.0
36	8894.0
37	9113.0
38	10106.5
39	11100.0
40	11821.0
41	12542.0
42	14248.5
43	15955.0
44	17599.5
45	19244.0
46	27546.0
47	35848.0
48	32791.0
49	29734.0
50	30331.5
51	30929.0
52	28694.5
53	26460.0
54	24821.5
55	23183.0
56	22141.0
57	21099.0
58	19855.5
59	18612.0
60	17756.0
61	16900.0
62	15250.5
63	13601.0
64	11797.0
65	9993.0
66	8822.0
67	7651.0
68	6369.0
69	5087.0
70	4320.5
71	3554.0
72	2680.5
73	1807.0
74	1616.5
75	1081.5
76	737.0
77	545.0
78	353.0
79	247.5
80	142.0
81	97.5
82	53.0
83	41.5
84	30.0
85	22.0
86	14.0
87	12.0
88	10.0
89	6.5
90	3.0
91	3.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	561361.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.30600318365781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.93313090267561	35.04890424534665
2	6.964924021698712	6.032460437152384
3	2.608744935817758	3.3892294948762505
4	1.4990090838531396	2.5966436863070417
5	1.0578615267549578	2.290587732275966
6	0.9137547853857229	2.3742640586998003
7	0.7437779003779009	2.254703368518979
8	0.6056630511944421	2.098307681860033
9	0.6110180201181891	2.381467349205953
>10	3.937637302991518	26.280357337717092
>50	0.07898799868811861	2.430634721481824
>100	0.040114323936907	2.8901844335555085
>500	0.003308397850466556	0.9542014290484414
>1k	0.001654198925233278	1.83909430835181
>5k	0.0	0.0
>10k+	4.135497313083195E-4	7.138959715602285
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39862	7.1009564255443465	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	3310	0.5896383966823489	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	2942	0.5240834329424381	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	2933	0.5224801865466251	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1084	0.19310212145125863	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	935	0.16655948667613174	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	768	0.1368103591093788	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT	736	0.13110992747982136	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	659	0.11739326387119875	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	589	0.10492356968154182	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	563	0.10029196898252639	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.125539536946813E-4	0.0	0.0	0.0684051795546894	0.0
2	7.125539536946813E-4	0.0	0.0	0.41915986326089627	0.0
3	7.125539536946813E-4	0.0	0.0	0.5766342870274208	0.0
4	7.125539536946813E-4	0.0	0.0	0.8463359585008577	0.0
5	8.906924421183517E-4	0.0	0.0	1.8994907020615968	0.0
6	8.906924421183517E-4	0.0	0.0	2.2415165998350437	0.0
7	8.906924421183517E-4	0.0	0.0	2.638943567508252	0.0
8	8.906924421183517E-4	0.0	0.0	3.1532293835873886	0.0
9	8.906924421183517E-4	0.0	0.0	3.3135540231686917	0.0
10	8.906924421183517E-4	0.0	0.0	4.223841699013647	0.0
11	8.906924421183517E-4	0.0	0.0	4.80350434034427	0.0
12	8.906924421183517E-4	0.0	0.0	5.9093880764784155	0.0
13	8.906924421183517E-4	0.0	0.0	6.139186726544951	0.0
14	8.906924421183517E-4	0.0	0.0	6.244110296226492	0.0
15	8.906924421183517E-4	0.0	0.0	6.498492057695493	0.0
16	8.906924421183517E-4	0.0	0.0	6.858688081288155	0.0
17	8.906924421183517E-4	0.0	0.0	7.21371808871653	0.0
18	8.906924421183517E-4	0.0	0.0	7.58210848277668	0.0
19	8.906924421183517E-4	0.0	0.0	7.975972680681416	0.0
20	8.906924421183517E-4	0.0	0.0	8.258500323321357	0.0
21	8.906924421183517E-4	0.0	0.0	8.551181859801448	0.0
22	8.906924421183517E-4	0.0	0.0	8.912090437347803	0.0
23	8.906924421183517E-4	0.0	0.0	9.20655335871213	0.0
24	8.906924421183517E-4	0.0	0.0	9.46236022808852	0.0
25	8.906924421183517E-4	0.0	0.0	9.696077924900376	0.0
26	8.906924421183517E-4	0.0	0.0	9.930508175665926	0.0
27	8.906924421183517E-4	0.0	0.0	10.206622832722616	0.0
28	8.906924421183517E-4	0.0	0.0	10.416648110574123	0.0
29	8.906924421183517E-4	0.0	0.0	10.654106715642875	0.0
30	8.906924421183517E-4	0.0	0.0	10.959970500266317	0.0
31	8.906924421183517E-4	0.0	0.0	11.20954252254788	0.0
32	8.906924421183517E-4	0.0	0.0	11.440588142033379	0.0
33	8.906924421183517E-4	0.0	0.0	11.695148041990805	0.0
34	8.906924421183517E-4	0.0	0.0	11.943294956364976	0.0
35	8.906924421183517E-4	0.0	0.0	12.212105935396297	0.0
36	8.906924421183517E-4	0.0	0.0	12.458827741863079	0.0
37	8.906924421183517E-4	0.0	0.0	12.710181149028879	0.0
38	8.906924421183517E-4	0.0	0.0	12.991105545273006	0.0
39	8.906924421183517E-4	0.0	0.0	13.410087270045478	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAAGCG	35	1.2104465E-7	45.000004	17
GTAACGG	30	2.1635096E-6	45.000004	2
GCTATCG	40	6.8048394E-9	45.0	1
CCATCGA	25	3.888247E-5	45.0	41
GTATGCG	20	7.0302E-4	45.0	1
AATGTCG	20	7.0302E-4	45.0	15
CGTTTTT	22220	0.0	44.46332	1
CGATGAA	540	0.0	42.916668	19
CGAATGC	80	0.0	42.1875	45
CCGATGA	555	0.0	41.351353	18
TACGGCT	430	0.0	41.337208	7
GCGATGC	60	3.6379788E-12	41.250004	9
ATCGAAT	55	6.002665E-11	40.909092	43
GTTTTTT	24415	0.0	40.585705	2
GGCGAAC	50	1.0786607E-9	40.5	32
GATGAAT	590	0.0	40.42373	20
ACCGATG	580	0.0	39.56897	17
ATGAATG	605	0.0	39.421486	21
ACACGAC	40	3.4540062E-7	39.375	26
CACGTGA	40	3.4540062E-7	39.375	43
>>END_MODULE