##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935687.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 394607 Sequences flagged as poor quality 0 Sequence length 51 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.366050272803065 33.0 31.0 34.0 30.0 34.0 2 32.561097497003345 34.0 31.0 34.0 31.0 34.0 3 32.41657649256096 34.0 31.0 34.0 30.0 34.0 4 36.04381574579265 37.0 35.0 37.0 35.0 37.0 5 36.08128593765443 37.0 35.0 37.0 35.0 37.0 6 36.10714204258921 37.0 36.0 37.0 35.0 37.0 7 36.272172059796205 37.0 37.0 37.0 35.0 37.0 8 36.030453083701 37.0 37.0 37.0 35.0 37.0 9 37.87078536366562 39.0 38.0 39.0 35.0 39.0 10 37.70107727435144 39.0 38.0 39.0 35.0 39.0 11 37.65982357130005 39.0 37.0 39.0 35.0 39.0 12 37.629013170065406 39.0 37.0 39.0 35.0 39.0 13 37.6423961054923 39.0 38.0 39.0 35.0 39.0 14 39.03964957540033 41.0 39.0 41.0 35.0 41.0 15 39.122896958239465 41.0 39.0 41.0 35.0 41.0 16 39.18178339461794 41.0 39.0 41.0 35.0 41.0 17 39.13717445458393 41.0 39.0 41.0 35.0 41.0 18 38.48811602429759 39.0 38.0 41.0 35.0 41.0 19 37.7873504524755 37.0 37.0 41.0 35.0 41.0 20 36.953824944818514 36.0 35.0 40.0 34.0 41.0 21 36.93303970786124 36.0 35.0 40.0 34.0 41.0 22 36.94326507132413 36.0 35.0 40.0 34.0 41.0 23 36.9464074382867 36.0 35.0 40.0 34.0 41.0 24 36.81441788919102 36.0 35.0 40.0 34.0 41.0 25 36.75545796197229 36.0 35.0 40.0 34.0 41.0 26 36.60524521866059 36.0 35.0 40.0 33.0 41.0 27 36.50827025369545 35.0 35.0 40.0 33.0 41.0 28 36.39688601570677 35.0 35.0 40.0 33.0 41.0 29 36.166005671465534 36.0 35.0 40.0 33.0 41.0 30 35.67744870212642 36.0 35.0 40.0 31.0 41.0 31 35.05355454920972 35.0 35.0 40.0 25.0 41.0 32 33.99019023990958 35.0 34.0 40.0 18.0 41.0 33 32.809415443719956 35.0 33.0 40.0 12.0 41.0 34 31.80971954374859 35.0 30.0 40.0 8.0 41.0 35 31.194069035774834 35.0 24.0 40.0 7.0 41.0 36 30.827653842937405 35.0 23.0 40.0 7.0 41.0 37 30.64232514881895 35.0 23.0 40.0 7.0 41.0 38 30.502249073128453 35.0 22.0 40.0 7.0 41.0 39 30.400563091886358 35.0 22.0 40.0 7.0 41.0 40 30.287301036220846 35.0 21.0 40.0 7.0 41.0 41 30.097846718380566 35.0 21.0 40.0 7.0 41.0 42 29.994237304457346 35.0 20.0 40.0 7.0 41.0 43 29.87626423251488 35.0 20.0 40.0 7.0 41.0 44 29.762029056757736 35.0 18.0 40.0 7.0 41.0 45 29.713565648860765 35.0 20.0 40.0 7.0 41.0 46 29.657291938561656 35.0 20.0 40.0 7.0 41.0 47 29.651810535545494 35.0 20.0 40.0 7.0 41.0 48 29.60059248822246 35.0 20.0 40.0 7.0 41.0 49 29.588185207054106 35.0 20.0 40.0 7.0 41.0 50 29.459700410788454 35.0 20.0 40.0 7.0 41.0 51 28.82463058181938 35.0 20.0 39.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 8.0 10 4.0 11 5.0 12 7.0 13 14.0 14 10.0 15 12.0 16 25.0 17 76.0 18 184.0 19 325.0 20 649.0 21 1024.0 22 1809.0 23 3094.0 24 5943.0 25 12245.0 26 20888.0 27 24923.0 28 21808.0 29 16778.0 30 12874.0 31 11041.0 32 10610.0 33 11331.0 34 17269.0 35 25923.0 36 26557.0 37 25237.0 38 47074.0 39 96756.0 40 99.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.07480353871067 13.458200183980518 15.829673573960928 44.637322703347884 2 57.40090773858548 16.413292212251683 15.599824635650153 10.585975413512685 3 19.624588514648753 16.214866943566637 53.66681280362487 10.493731738159738 4 17.582303405666906 16.111726350520897 54.79248974295945 11.513480500852747 5 16.638833066823448 18.225981799613287 52.417721936002145 12.717463197561118 6 15.634542722252773 22.116941666012007 52.62197578856939 9.626539823165833 7 49.945895536571825 3.276677808553827 43.34287024812028 3.4345564067540613 8 50.09465113391298 4.06708446631712 42.06767746137296 3.770586938396937 9 46.4079451200815 4.552630845372738 43.45310650850086 5.5863175260449 10 25.37359955601396 17.00476676794887 48.18338245393518 9.43825122210199 11 18.32354722546736 15.818016406196545 53.16403408961321 12.69440227872288 12 16.67988657068932 14.218196838880202 55.234195034553366 13.867721555877113 13 15.350716028859093 15.711327979483386 56.85479477049318 12.083161221164348 14 12.674382360171006 18.716849929170035 54.8586314991878 13.750136211471158 15 11.617888177351137 17.11449619494839 58.3314538262119 12.936161801488568 16 13.490637520368368 16.345376539189623 56.117859034431724 14.046126906010285 17 12.921970466818886 16.886928006852386 54.747634988735626 15.443466537593098 18 14.248100008362751 16.178628356820838 55.83428575772857 13.738985877087837 19 14.021798903719397 18.151223875906915 53.87841574021748 13.948561480156204 20 15.393796866249204 17.199644202966496 54.95569009166081 12.450868839123483 21 14.478202363364057 18.1418474583573 55.0322219322009 12.347728246077743 22 13.416639846733586 16.00225034021191 54.75042257233146 15.830687240723048 23 12.90093688150489 17.50627839850788 54.94175217368167 14.651032546305565 24 14.010648569336073 16.202956359111724 54.81884507877458 14.967549992777624 25 12.505353427587448 18.181380462079993 54.09838142759759 15.214884682734976 26 12.1931440648544 18.040987615526333 54.91438317110442 14.85148514851485 27 13.303362586066644 18.025529197404 54.45797971145976 14.2131285050696 28 12.227101901385428 17.71762791840996 55.308699541569204 14.7465706386354 29 12.644479190688457 16.413292212251683 55.27499512172871 15.667233475331152 30 13.719979625298082 18.177325795031514 53.83381440268419 14.268880176986217 31 15.73464231501215 17.990050860729788 50.687392773062825 15.58791405119524 32 15.666473225259562 19.889662372943206 49.18083054786154 15.263033853935687 33 15.740217482203814 21.331603342059317 46.79415215644933 16.134027019287544 34 16.212586193351868 21.428155101151273 45.480440032741434 16.878818672755425 35 16.557739725853825 22.994523665317644 42.94906071103655 17.49867589779198 36 17.348653216998176 25.728636339446588 41.305400056258506 15.61731038729673 37 17.555441236470717 25.771970593527232 40.923247686939156 15.749340483062896 38 17.453060893496566 24.89160101062576 39.83710375132727 17.8182343445504 39 17.95837377441353 23.389346869163496 40.78057409017073 17.871705266252246 40 18.199626463798158 23.207140268672376 41.49850357444242 17.094729693087046 41 16.90466717518949 25.689863585795486 39.18100793954491 18.224461299470104 42 17.81722067778828 25.101430030384662 39.48409430141888 17.597254990408178 43 17.543784068706334 25.076595194712713 39.17999427278279 18.199626463798158 44 16.89148950728193 24.21599211367259 39.31988028595539 19.572638093090085 45 17.378049553099668 22.472231866135168 39.71901157354026 20.43070700722491 46 18.426434401822572 23.55609505153228 40.115101860838756 17.902368685806387 47 16.445476131949004 23.01454358386952 42.60340034515353 17.936579939027943 48 16.515672555225834 22.300668766646307 41.84010927327695 19.3435494048509 49 16.838525418961144 21.183354578099223 43.0362360525789 18.94188395036074 50 15.87148732789839 20.914226052756288 42.89584320602523 20.31844341332009 51 15.889733329616554 20.95401247316951 40.96049994044708 22.195754256766858 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 115.0 1 250.5 2 386.0 3 24237.5 4 48089.0 5 31531.0 6 14973.0 7 14312.0 8 13651.0 9 13215.0 10 12779.0 11 12264.5 12 11750.0 13 11008.0 14 10266.0 15 9439.0 16 8612.0 17 8012.0 18 7412.0 19 6735.5 20 6059.0 21 5385.5 22 4712.0 23 4400.5 24 4089.0 25 3923.5 26 3856.0 27 3954.0 28 4131.5 29 4309.0 30 4676.0 31 5043.0 32 5336.5 33 5630.0 34 6222.5 35 6815.0 36 7544.0 37 8273.0 38 9003.0 39 9733.0 40 10615.0 41 11497.0 42 13106.0 43 14715.0 44 16419.0 45 18123.0 46 22922.5 47 27722.0 48 28283.0 49 28844.0 50 28233.0 51 27622.0 52 23213.0 53 18804.0 54 16054.0 55 13304.0 56 11864.5 57 10425.0 58 9732.5 59 9040.0 60 8247.5 61 7455.0 62 6964.5 63 6474.0 64 5239.0 65 4004.0 66 3233.0 67 2462.0 68 1932.0 69 1402.0 70 1219.0 71 1036.0 72 763.0 73 490.0 74 414.5 75 288.5 76 238.0 77 179.5 78 121.0 79 74.0 80 27.0 81 32.5 82 38.0 83 23.0 84 8.0 85 6.5 86 5.0 87 3.5 88 2.0 89 1.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 394607.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.17700538936076 #Duplication Level Percentage of deduplicated Percentage of total 1 81.64619895667182 38.51823168199728 2 8.01444588133168 7.561951130726495 3 2.7089064445096582 3.833942817957188 4 1.3938776273927456 2.6303588935846776 5 0.8851987326027334 2.0880512689327233 6 0.7255397003493546 2.0537274212146013 7 0.5795070633756391 1.9137585496431606 8 0.4930320306008981 1.860781981182884 9 0.40308654677086 1.7114774570448914 >10 3.0202393101937437 22.929226874854827 >50 0.09422658699562476 3.0470936477804833 >100 0.0314088623318749 2.6638756992879253 >500 0.0016245963275107707 0.5819803244440294 >1k 0.0021661284366810275 1.1478654950513711 >5k 0.0 0.0 >10k+ 5.415321091702569E-4 7.457676756297481 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29191 7.397486613263323 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC 1166 0.2954838611580636 No Hit GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC 1153 0.29218944418117265 No Hit GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC 1108 0.28078569310731943 No Hit CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC 1066 0.2701421921050564 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG 987 0.2501222735531808 No Hit CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT 684 0.17333701632256904 Illumina Single End Adapter 2 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 607 0.15382393115175352 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 453 0.11479776081012248 No Hit CGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTG 427 0.10820892685634062 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 420 0.10643501002263012 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA 402 0.10187350959308883 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1809395170384712 0.0 2 0.0 0.0 0.0 0.9330802545317239 0.0 3 0.0 0.0 0.0 1.3208077910427336 0.0 4 0.0 0.0 0.0 1.7749305004726221 0.0 5 0.0 0.0 0.0 3.1013134587070175 0.0 6 0.0 0.0 0.0 3.7746416054454177 0.0 7 0.0 0.0 0.0 4.423641749892932 0.0 8 0.0 0.0 0.0 5.417288593461342 0.0 9 0.0 0.0 0.0 5.76294895934436 0.0 10 0.0 0.0 0.0 6.82071022561688 0.0 11 0.0 0.0 0.0 8.038884256994935 0.0 12 0.0 0.0 0.0 9.274797456710093 0.0 13 0.0 0.0 0.0 9.699777246729024 0.0 14 0.0 0.0 0.0 9.872860846361062 0.0 15 0.0 0.0 0.0 10.200782043906976 0.0 16 0.0 0.0 0.0 10.803406933987485 0.0 17 0.0 0.0 0.0 11.59305334167919 0.0 18 0.0 0.0 0.0 12.351529496435694 0.0 19 0.0 0.0 0.0 12.942750635442351 0.0 20 0.0 0.0 0.0 13.419174013638886 0.0 21 0.0 0.0 0.0 14.027120654220528 0.0 22 0.0 0.0 0.0 14.682202799240764 0.0 23 0.0 0.0 0.0 15.320812859376545 0.0 24 0.0 0.0 0.0 15.802304571383681 0.0 25 0.0 0.0 0.0 16.242489362834416 0.0 26 0.0 0.0 0.0 16.641367233728747 0.0 27 0.0 0.0 0.0 17.049114688791633 0.0 28 2.5341669053007167E-4 0.0 0.0 17.46117022759353 0.0 29 2.5341669053007167E-4 0.0 0.0 17.892485434875713 0.0 30 2.5341669053007167E-4 0.0 0.0 18.369415646453305 0.0 31 5.068333810601433E-4 0.0 0.0 18.84203777429189 0.0 32 5.068333810601433E-4 0.0 0.0 19.269298314525592 0.0 33 5.068333810601433E-4 0.0 0.0 19.66361468499038 0.0 34 5.068333810601433E-4 0.0 0.0 20.035883803379058 0.0 35 7.602500715902151E-4 0.0 0.0 20.450726925776785 0.0 36 7.602500715902151E-4 0.0 0.0 20.875453299105185 0.0 37 7.602500715902151E-4 0.0 0.0 21.309049256602137 0.0 38 7.602500715902151E-4 0.0 0.0 21.715022794831313 0.0 39 7.602500715902151E-4 0.0 0.0 22.132400084134343 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCTAC 25 3.8861333E-5 45.000004 41 TAGCTAG 25 3.8861333E-5 45.000004 1 CGCATAT 20 7.027651E-4 45.0 26 ATTACGG 20 7.027651E-4 45.0 2 TCGATGG 20 7.027651E-4 45.0 2 GCTTGAC 20 7.027651E-4 45.0 43 TATTGCG 20 7.027651E-4 45.0 1 GAGCGTC 20 7.027651E-4 45.0 15 CGCTAAG 20 7.027651E-4 45.0 3 TCGCTAA 20 7.027651E-4 45.0 2 TCATCGC 20 7.027651E-4 45.0 22 CATCGCA 20 7.027651E-4 45.0 23 ATGCGAG 20 7.027651E-4 45.0 1 GCTCATG 20 7.027651E-4 45.0 1 GAGTAAG 30 2.1618653E-6 44.999996 1 GGCCGAT 30 2.1618653E-6 44.999996 8 ATAGCGG 30 2.1618653E-6 44.999996 2 CGTTTTT 14490 0.0 44.673916 1 TAGGGCG 75 0.0 42.0 5 ACGGGCT 70 0.0 41.785717 5 >>END_MODULE