##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935687.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394607
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.366050272803065	33.0	31.0	34.0	30.0	34.0
2	32.561097497003345	34.0	31.0	34.0	31.0	34.0
3	32.41657649256096	34.0	31.0	34.0	30.0	34.0
4	36.04381574579265	37.0	35.0	37.0	35.0	37.0
5	36.08128593765443	37.0	35.0	37.0	35.0	37.0
6	36.10714204258921	37.0	36.0	37.0	35.0	37.0
7	36.272172059796205	37.0	37.0	37.0	35.0	37.0
8	36.030453083701	37.0	37.0	37.0	35.0	37.0
9	37.87078536366562	39.0	38.0	39.0	35.0	39.0
10	37.70107727435144	39.0	38.0	39.0	35.0	39.0
11	37.65982357130005	39.0	37.0	39.0	35.0	39.0
12	37.629013170065406	39.0	37.0	39.0	35.0	39.0
13	37.6423961054923	39.0	38.0	39.0	35.0	39.0
14	39.03964957540033	41.0	39.0	41.0	35.0	41.0
15	39.122896958239465	41.0	39.0	41.0	35.0	41.0
16	39.18178339461794	41.0	39.0	41.0	35.0	41.0
17	39.13717445458393	41.0	39.0	41.0	35.0	41.0
18	38.48811602429759	39.0	38.0	41.0	35.0	41.0
19	37.7873504524755	37.0	37.0	41.0	35.0	41.0
20	36.953824944818514	36.0	35.0	40.0	34.0	41.0
21	36.93303970786124	36.0	35.0	40.0	34.0	41.0
22	36.94326507132413	36.0	35.0	40.0	34.0	41.0
23	36.9464074382867	36.0	35.0	40.0	34.0	41.0
24	36.81441788919102	36.0	35.0	40.0	34.0	41.0
25	36.75545796197229	36.0	35.0	40.0	34.0	41.0
26	36.60524521866059	36.0	35.0	40.0	33.0	41.0
27	36.50827025369545	35.0	35.0	40.0	33.0	41.0
28	36.39688601570677	35.0	35.0	40.0	33.0	41.0
29	36.166005671465534	36.0	35.0	40.0	33.0	41.0
30	35.67744870212642	36.0	35.0	40.0	31.0	41.0
31	35.05355454920972	35.0	35.0	40.0	25.0	41.0
32	33.99019023990958	35.0	34.0	40.0	18.0	41.0
33	32.809415443719956	35.0	33.0	40.0	12.0	41.0
34	31.80971954374859	35.0	30.0	40.0	8.0	41.0
35	31.194069035774834	35.0	24.0	40.0	7.0	41.0
36	30.827653842937405	35.0	23.0	40.0	7.0	41.0
37	30.64232514881895	35.0	23.0	40.0	7.0	41.0
38	30.502249073128453	35.0	22.0	40.0	7.0	41.0
39	30.400563091886358	35.0	22.0	40.0	7.0	41.0
40	30.287301036220846	35.0	21.0	40.0	7.0	41.0
41	30.097846718380566	35.0	21.0	40.0	7.0	41.0
42	29.994237304457346	35.0	20.0	40.0	7.0	41.0
43	29.87626423251488	35.0	20.0	40.0	7.0	41.0
44	29.762029056757736	35.0	18.0	40.0	7.0	41.0
45	29.713565648860765	35.0	20.0	40.0	7.0	41.0
46	29.657291938561656	35.0	20.0	40.0	7.0	41.0
47	29.651810535545494	35.0	20.0	40.0	7.0	41.0
48	29.60059248822246	35.0	20.0	40.0	7.0	41.0
49	29.588185207054106	35.0	20.0	40.0	7.0	41.0
50	29.459700410788454	35.0	20.0	40.0	7.0	41.0
51	28.82463058181938	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	4.0
11	5.0
12	7.0
13	14.0
14	10.0
15	12.0
16	25.0
17	76.0
18	184.0
19	325.0
20	649.0
21	1024.0
22	1809.0
23	3094.0
24	5943.0
25	12245.0
26	20888.0
27	24923.0
28	21808.0
29	16778.0
30	12874.0
31	11041.0
32	10610.0
33	11331.0
34	17269.0
35	25923.0
36	26557.0
37	25237.0
38	47074.0
39	96756.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.07480353871067	13.458200183980518	15.829673573960928	44.637322703347884
2	57.40090773858548	16.413292212251683	15.599824635650153	10.585975413512685
3	19.624588514648753	16.214866943566637	53.66681280362487	10.493731738159738
4	17.582303405666906	16.111726350520897	54.79248974295945	11.513480500852747
5	16.638833066823448	18.225981799613287	52.417721936002145	12.717463197561118
6	15.634542722252773	22.116941666012007	52.62197578856939	9.626539823165833
7	49.945895536571825	3.276677808553827	43.34287024812028	3.4345564067540613
8	50.09465113391298	4.06708446631712	42.06767746137296	3.770586938396937
9	46.4079451200815	4.552630845372738	43.45310650850086	5.5863175260449
10	25.37359955601396	17.00476676794887	48.18338245393518	9.43825122210199
11	18.32354722546736	15.818016406196545	53.16403408961321	12.69440227872288
12	16.67988657068932	14.218196838880202	55.234195034553366	13.867721555877113
13	15.350716028859093	15.711327979483386	56.85479477049318	12.083161221164348
14	12.674382360171006	18.716849929170035	54.8586314991878	13.750136211471158
15	11.617888177351137	17.11449619494839	58.3314538262119	12.936161801488568
16	13.490637520368368	16.345376539189623	56.117859034431724	14.046126906010285
17	12.921970466818886	16.886928006852386	54.747634988735626	15.443466537593098
18	14.248100008362751	16.178628356820838	55.83428575772857	13.738985877087837
19	14.021798903719397	18.151223875906915	53.87841574021748	13.948561480156204
20	15.393796866249204	17.199644202966496	54.95569009166081	12.450868839123483
21	14.478202363364057	18.1418474583573	55.0322219322009	12.347728246077743
22	13.416639846733586	16.00225034021191	54.75042257233146	15.830687240723048
23	12.90093688150489	17.50627839850788	54.94175217368167	14.651032546305565
24	14.010648569336073	16.202956359111724	54.81884507877458	14.967549992777624
25	12.505353427587448	18.181380462079993	54.09838142759759	15.214884682734976
26	12.1931440648544	18.040987615526333	54.91438317110442	14.85148514851485
27	13.303362586066644	18.025529197404	54.45797971145976	14.2131285050696
28	12.227101901385428	17.71762791840996	55.308699541569204	14.7465706386354
29	12.644479190688457	16.413292212251683	55.27499512172871	15.667233475331152
30	13.719979625298082	18.177325795031514	53.83381440268419	14.268880176986217
31	15.73464231501215	17.990050860729788	50.687392773062825	15.58791405119524
32	15.666473225259562	19.889662372943206	49.18083054786154	15.263033853935687
33	15.740217482203814	21.331603342059317	46.79415215644933	16.134027019287544
34	16.212586193351868	21.428155101151273	45.480440032741434	16.878818672755425
35	16.557739725853825	22.994523665317644	42.94906071103655	17.49867589779198
36	17.348653216998176	25.728636339446588	41.305400056258506	15.61731038729673
37	17.555441236470717	25.771970593527232	40.923247686939156	15.749340483062896
38	17.453060893496566	24.89160101062576	39.83710375132727	17.8182343445504
39	17.95837377441353	23.389346869163496	40.78057409017073	17.871705266252246
40	18.199626463798158	23.207140268672376	41.49850357444242	17.094729693087046
41	16.90466717518949	25.689863585795486	39.18100793954491	18.224461299470104
42	17.81722067778828	25.101430030384662	39.48409430141888	17.597254990408178
43	17.543784068706334	25.076595194712713	39.17999427278279	18.199626463798158
44	16.89148950728193	24.21599211367259	39.31988028595539	19.572638093090085
45	17.378049553099668	22.472231866135168	39.71901157354026	20.43070700722491
46	18.426434401822572	23.55609505153228	40.115101860838756	17.902368685806387
47	16.445476131949004	23.01454358386952	42.60340034515353	17.936579939027943
48	16.515672555225834	22.300668766646307	41.84010927327695	19.3435494048509
49	16.838525418961144	21.183354578099223	43.0362360525789	18.94188395036074
50	15.87148732789839	20.914226052756288	42.89584320602523	20.31844341332009
51	15.889733329616554	20.95401247316951	40.96049994044708	22.195754256766858
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	115.0
1	250.5
2	386.0
3	24237.5
4	48089.0
5	31531.0
6	14973.0
7	14312.0
8	13651.0
9	13215.0
10	12779.0
11	12264.5
12	11750.0
13	11008.0
14	10266.0
15	9439.0
16	8612.0
17	8012.0
18	7412.0
19	6735.5
20	6059.0
21	5385.5
22	4712.0
23	4400.5
24	4089.0
25	3923.5
26	3856.0
27	3954.0
28	4131.5
29	4309.0
30	4676.0
31	5043.0
32	5336.5
33	5630.0
34	6222.5
35	6815.0
36	7544.0
37	8273.0
38	9003.0
39	9733.0
40	10615.0
41	11497.0
42	13106.0
43	14715.0
44	16419.0
45	18123.0
46	22922.5
47	27722.0
48	28283.0
49	28844.0
50	28233.0
51	27622.0
52	23213.0
53	18804.0
54	16054.0
55	13304.0
56	11864.5
57	10425.0
58	9732.5
59	9040.0
60	8247.5
61	7455.0
62	6964.5
63	6474.0
64	5239.0
65	4004.0
66	3233.0
67	2462.0
68	1932.0
69	1402.0
70	1219.0
71	1036.0
72	763.0
73	490.0
74	414.5
75	288.5
76	238.0
77	179.5
78	121.0
79	74.0
80	27.0
81	32.5
82	38.0
83	23.0
84	8.0
85	6.5
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	394607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.17700538936076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64619895667182	38.51823168199728
2	8.01444588133168	7.561951130726495
3	2.7089064445096582	3.833942817957188
4	1.3938776273927456	2.6303588935846776
5	0.8851987326027334	2.0880512689327233
6	0.7255397003493546	2.0537274212146013
7	0.5795070633756391	1.9137585496431606
8	0.4930320306008981	1.860781981182884
9	0.40308654677086	1.7114774570448914
>10	3.0202393101937437	22.929226874854827
>50	0.09422658699562476	3.0470936477804833
>100	0.0314088623318749	2.6638756992879253
>500	0.0016245963275107707	0.5819803244440294
>1k	0.0021661284366810275	1.1478654950513711
>5k	0.0	0.0
>10k+	5.415321091702569E-4	7.457676756297481
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29191	7.397486613263323	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC	1166	0.2954838611580636	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	1153	0.29218944418117265	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC	1108	0.28078569310731943	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	1066	0.2701421921050564	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG	987	0.2501222735531808	No Hit
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT	684	0.17333701632256904	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	607	0.15382393115175352	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	453	0.11479776081012248	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTG	427	0.10820892685634062	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	420	0.10643501002263012	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	402	0.10187350959308883	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1809395170384712	0.0
2	0.0	0.0	0.0	0.9330802545317239	0.0
3	0.0	0.0	0.0	1.3208077910427336	0.0
4	0.0	0.0	0.0	1.7749305004726221	0.0
5	0.0	0.0	0.0	3.1013134587070175	0.0
6	0.0	0.0	0.0	3.7746416054454177	0.0
7	0.0	0.0	0.0	4.423641749892932	0.0
8	0.0	0.0	0.0	5.417288593461342	0.0
9	0.0	0.0	0.0	5.76294895934436	0.0
10	0.0	0.0	0.0	6.82071022561688	0.0
11	0.0	0.0	0.0	8.038884256994935	0.0
12	0.0	0.0	0.0	9.274797456710093	0.0
13	0.0	0.0	0.0	9.699777246729024	0.0
14	0.0	0.0	0.0	9.872860846361062	0.0
15	0.0	0.0	0.0	10.200782043906976	0.0
16	0.0	0.0	0.0	10.803406933987485	0.0
17	0.0	0.0	0.0	11.59305334167919	0.0
18	0.0	0.0	0.0	12.351529496435694	0.0
19	0.0	0.0	0.0	12.942750635442351	0.0
20	0.0	0.0	0.0	13.419174013638886	0.0
21	0.0	0.0	0.0	14.027120654220528	0.0
22	0.0	0.0	0.0	14.682202799240764	0.0
23	0.0	0.0	0.0	15.320812859376545	0.0
24	0.0	0.0	0.0	15.802304571383681	0.0
25	0.0	0.0	0.0	16.242489362834416	0.0
26	0.0	0.0	0.0	16.641367233728747	0.0
27	0.0	0.0	0.0	17.049114688791633	0.0
28	2.5341669053007167E-4	0.0	0.0	17.46117022759353	0.0
29	2.5341669053007167E-4	0.0	0.0	17.892485434875713	0.0
30	2.5341669053007167E-4	0.0	0.0	18.369415646453305	0.0
31	5.068333810601433E-4	0.0	0.0	18.84203777429189	0.0
32	5.068333810601433E-4	0.0	0.0	19.269298314525592	0.0
33	5.068333810601433E-4	0.0	0.0	19.66361468499038	0.0
34	5.068333810601433E-4	0.0	0.0	20.035883803379058	0.0
35	7.602500715902151E-4	0.0	0.0	20.450726925776785	0.0
36	7.602500715902151E-4	0.0	0.0	20.875453299105185	0.0
37	7.602500715902151E-4	0.0	0.0	21.309049256602137	0.0
38	7.602500715902151E-4	0.0	0.0	21.715022794831313	0.0
39	7.602500715902151E-4	0.0	0.0	22.132400084134343	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAC	25	3.8861333E-5	45.000004	41
TAGCTAG	25	3.8861333E-5	45.000004	1
CGCATAT	20	7.027651E-4	45.0	26
ATTACGG	20	7.027651E-4	45.0	2
TCGATGG	20	7.027651E-4	45.0	2
GCTTGAC	20	7.027651E-4	45.0	43
TATTGCG	20	7.027651E-4	45.0	1
GAGCGTC	20	7.027651E-4	45.0	15
CGCTAAG	20	7.027651E-4	45.0	3
TCGCTAA	20	7.027651E-4	45.0	2
TCATCGC	20	7.027651E-4	45.0	22
CATCGCA	20	7.027651E-4	45.0	23
ATGCGAG	20	7.027651E-4	45.0	1
GCTCATG	20	7.027651E-4	45.0	1
GAGTAAG	30	2.1618653E-6	44.999996	1
GGCCGAT	30	2.1618653E-6	44.999996	8
ATAGCGG	30	2.1618653E-6	44.999996	2
CGTTTTT	14490	0.0	44.673916	1
TAGGGCG	75	0.0	42.0	5
ACGGGCT	70	0.0	41.785717	5
>>END_MODULE