FastQCFastQC Report
Sat 14 Jan 2017
SRR2935686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779635
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT549847.052530992066801No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11310.14506788433048798No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10130.12993259666382345No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTG9410.12069750588416374No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA8720.11184721055365653No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG253.889647E-545.01
CGAATCC207.031889E-445.019
GCGATTC207.031889E-445.09
CACGTCA253.889647E-545.034
ACGAAAC207.031889E-445.029
CGTTTTT303500.044.5255361
GTTTTTT337250.040.2498172
TAGACGG403.4563163E-739.3752
GTCGAAG301.139585E-437.4999961
CCATACG301.139585E-437.4999962
ACAACGA1150.037.17391613
GCTACGA850.037.05882310
TGACGGC250.002106693636.014
TCGATAG250.002106693636.01
GCTTCGA250.002106693636.024
ACGCTGG250.002106693636.02
ACTCGCT250.002106693636.041
GTGCCGT504.8754373E-836.042
GGCGATT1950.035.769238
AGGGATG4850.035.721656
Produced by FastQC (version 0.11.5)