##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935686.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	779635
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.436273384340105	34.0	31.0	34.0	31.0	34.0
2	32.64866764575731	34.0	31.0	34.0	31.0	34.0
3	32.47594836045072	34.0	31.0	34.0	31.0	34.0
4	36.08750633309177	37.0	35.0	37.0	35.0	37.0
5	36.10659988327871	37.0	35.0	37.0	35.0	37.0
6	36.11378529696589	37.0	36.0	37.0	35.0	37.0
7	36.25963175075516	37.0	37.0	37.0	35.0	37.0
8	36.0666055269453	37.0	37.0	37.0	35.0	37.0
9	37.86583978400149	39.0	38.0	39.0	35.0	39.0
10	37.69136839674976	39.0	38.0	39.0	35.0	39.0
11	37.64578937579765	39.0	37.0	39.0	35.0	39.0
12	37.51929941575224	39.0	37.0	39.0	35.0	39.0
13	37.4965285037229	39.0	37.0	39.0	35.0	39.0
14	38.78749799585704	41.0	38.0	41.0	35.0	41.0
15	38.91633905609676	41.0	38.0	41.0	35.0	41.0
16	38.98983626953638	41.0	39.0	41.0	35.0	41.0
17	38.95181847916012	41.0	38.0	41.0	35.0	41.0
18	38.26697493057649	39.0	38.0	40.0	35.0	41.0
19	37.56658051524111	37.0	37.0	40.0	35.0	41.0
20	36.74351459336741	35.0	35.0	40.0	34.0	41.0
21	36.68695479294798	35.0	35.0	40.0	33.0	41.0
22	36.68352883079903	35.0	35.0	40.0	33.0	41.0
23	36.69909508936874	35.0	35.0	40.0	34.0	41.0
24	36.53617013089458	35.0	35.0	40.0	33.0	41.0
25	36.44773259281587	35.0	35.0	40.0	33.0	41.0
26	36.30677945448832	35.0	35.0	40.0	33.0	41.0
27	36.23597837449576	35.0	35.0	40.0	33.0	41.0
28	36.08756405240914	35.0	35.0	40.0	33.0	41.0
29	35.808541176319686	35.0	35.0	40.0	33.0	41.0
30	35.26119145497572	35.0	35.0	40.0	30.0	41.0
31	34.54630051241927	35.0	35.0	40.0	23.0	41.0
32	33.45099052761869	35.0	33.0	40.0	17.0	41.0
33	32.196298267779156	35.0	31.0	40.0	11.0	41.0
34	31.11339408825925	35.0	25.0	40.0	7.0	41.0
35	30.483957236399085	35.0	23.0	40.0	7.0	41.0
36	30.122253362150236	35.0	21.0	40.0	7.0	41.0
37	29.941458503017436	35.0	21.0	40.0	7.0	41.0
38	29.78531235770585	35.0	20.0	40.0	7.0	41.0
39	29.650217088765896	35.0	19.0	40.0	7.0	41.0
40	29.518897945833626	35.0	18.0	40.0	7.0	41.0
41	29.34050421030354	35.0	18.0	40.0	7.0	41.0
42	29.247184900626575	35.0	18.0	40.0	7.0	41.0
43	29.14840790882913	35.0	18.0	40.0	7.0	41.0
44	29.040447132311915	35.0	18.0	40.0	7.0	41.0
45	28.988966631821302	35.0	18.0	40.0	7.0	41.0
46	28.956869560755994	35.0	18.0	40.0	7.0	41.0
47	28.972337055160427	35.0	18.0	40.0	7.0	41.0
48	28.91466134793846	35.0	18.0	40.0	7.0	41.0
49	28.88197938779044	35.0	18.0	39.0	7.0	41.0
50	28.753099848005796	35.0	18.0	39.0	7.0	41.0
51	28.116820050408204	34.0	18.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	4.0
10	23.0
11	18.0
12	16.0
13	38.0
14	30.0
15	75.0
16	93.0
17	223.0
18	458.0
19	922.0
20	1551.0
21	2499.0
22	3855.0
23	6614.0
24	13109.0
25	26497.0
26	43946.0
27	51166.0
28	46019.0
29	34924.0
30	27049.0
31	23297.0
32	21875.0
33	25380.0
34	43108.0
35	51520.0
36	50466.0
37	50680.0
38	91022.0
39	163012.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.03248314916596	12.952599613921901	14.698929627325608	47.315987609586536
2	60.30963207141804	14.333887011229615	14.696749119780412	10.65973179757194
3	19.636496565700618	14.686744438102444	54.446247282382146	11.230511713814797
4	17.97969562679972	16.102022100085296	53.97628377381724	11.941998499297748
5	16.072008055051402	17.745226933116136	53.85520147248391	12.327563539348542
6	16.3967754141361	20.63504075625132	53.356763100681725	9.611420728930845
7	49.43210604962579	3.6907014179712303	43.43519723973398	3.4419952926690054
8	50.39614691490249	3.0828528734600167	43.40287442200517	3.118125789632328
9	46.87257498701315	4.142707805575687	44.77890294817446	4.205814259236694
10	26.996992182239126	14.482289789452757	49.12042173581227	9.400296292495849
11	20.162640209841783	15.633341243017565	52.82715629749819	11.37686224964246
12	18.78635515337305	13.986673250944351	54.98380652484817	12.24316507083443
13	16.90957948270665	13.887780820512162	56.18654883374913	13.016090863032058
14	13.312768154328628	15.128617878879218	57.843093242350584	13.715520724441566
15	13.449627069077197	15.430169245865052	58.33627274301436	12.783930942043392
16	16.614826168655846	15.834589262924318	55.9583651324017	11.592219436018137
17	16.23336561339601	15.768276180520372	55.11296953061369	12.88538867546993
18	16.55005226804851	15.528163820249219	55.63988276565316	12.281901146049112
19	15.930403329763287	17.203819736158586	54.403663252675926	12.462113681402194
20	16.31430092286775	17.647488889031408	54.417131093396264	11.621079094704573
21	15.952336670364978	16.823898362695363	55.88025165622375	11.343513310715911
22	15.39964214023229	14.902999480526145	56.64484021368974	13.05251816555183
23	14.825399064947058	15.650785303379145	56.40511264886774	13.118702982806058
24	15.162992939003509	15.508667517492158	55.73851866578591	13.58982087771842
25	15.410544677958276	15.947975655274584	54.31682774631719	14.324651920449954
26	13.974103266272037	16.448851064921406	54.47138725172677	15.105658417079788
27	13.730784277257948	16.021856381511864	55.69708902242716	14.55027031880303
28	13.314948661873826	16.943313217082352	54.63005124192731	15.11168687911651
29	15.060252554079796	17.224726955562538	53.83634649547545	13.87867399488222
30	16.14999326607964	16.048535532653098	53.588666491370965	14.212804709896298
31	16.485406632590895	17.219852879873272	52.08796423967625	14.206776247859576
32	18.068968171003096	18.140796654844895	49.59064177467661	14.199593399475397
33	17.62786432112463	20.220231262064942	47.28674315545095	14.865161261359482
34	16.735780204839443	21.54187536475402	46.38285864539176	15.339485785014784
35	16.9612703380428	24.141168623779077	43.27358315108994	15.62397788708819
36	18.814573486310902	25.34429572812919	40.4611132132344	15.38001757232551
37	18.637695844850473	25.501677066832556	40.188421504935	15.672205583381968
38	18.673610086771376	25.441905507064206	40.430073047002764	15.45441135916166
39	18.771091600556673	24.15514952509828	39.899440122621485	17.174318751723565
40	19.70447709505089	23.52472631423679	40.38838687334458	16.382409717367743
41	18.020355679260167	24.307143727513516	39.73705644307913	17.935444150147184
42	18.63500227670641	24.23659789516889	39.47770431035036	17.65069551777434
43	18.37013474253978	23.740340030911902	39.76668569266387	18.122839533884445
44	18.784174645827857	23.635162608143553	39.124846883477524	18.455815862551063
45	18.373341371282713	23.34271806678766	39.391766660039636	18.892173901889986
46	17.62016841214158	23.722254644801737	40.17200356577116	18.485573377285526
47	16.898548679830945	23.33835705169727	41.70643955184157	18.05665471663022
48	16.77644025729989	23.012307041115392	42.30684871766917	17.90440398391555
49	17.974308490511586	22.51720356320586	41.795199035446075	17.713288910836482
50	16.53209514708806	22.30146158138103	42.33391266425956	18.83253060727135
51	16.193988212432743	22.621867925375337	41.734273089330266	19.44987077286166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	207.0
1	470.0
2	733.0
3	49564.5
4	98396.0
5	64428.5
6	30461.0
7	29010.5
8	27560.0
9	26781.0
10	26002.0
11	25029.0
12	24056.0
13	22580.5
14	21105.0
15	19645.5
16	18186.0
17	16532.0
18	14878.0
19	13540.5
20	12203.0
21	11129.5
22	10056.0
23	9425.0
24	8794.0
25	8427.0
26	7962.5
27	7865.0
28	8115.0
29	8365.0
30	9069.0
31	9773.0
32	10801.0
33	11829.0
34	13024.0
35	14219.0
36	15267.0
37	16315.0
38	17377.0
39	18439.0
40	19603.5
41	20768.0
42	22276.0
43	23784.0
44	25718.0
45	27652.0
46	31775.0
47	35898.0
48	38526.0
49	41154.0
50	42138.0
51	43122.0
52	39736.5
53	36351.0
54	33616.0
55	30881.0
56	29187.5
57	27494.0
58	26857.5
59	26221.0
60	23954.0
61	21687.0
62	19826.0
63	17965.0
64	15923.5
65	13882.0
66	11567.5
67	9253.0
68	8036.5
69	6820.0
70	5465.0
71	4110.0
72	3204.0
73	2298.0
74	1848.0
75	1086.5
76	775.0
77	532.5
78	290.0
79	231.5
80	173.0
81	139.0
82	105.0
83	65.5
84	26.0
85	18.0
86	10.0
87	8.5
88	7.0
89	6.5
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	779635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.7394442346346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15653406753968	34.62262807654105
2	7.23028918934011	6.3249766159484615
3	2.969305435609703	3.8962730854934393
4	1.8669055753878863	3.266296492240275
5	1.3669898781429666	2.9895688772172115
6	1.1151993071644513	2.926691874373359
7	0.9151717191652132	2.802037166287906
8	0.8265536541804586	2.8922397971167695
9	0.6913414443597088	2.72150014973969
>10	3.7611281487548665	24.055958944725358
>50	0.052065781707545035	1.5779553467662182
>100	0.04407837803942469	3.4888380411071513
>500	0.0035499364863837805	1.0430413325411396
>1k	5.916560810639634E-4	0.27741975151571807
>5k	0.0	0.0
>10k+	2.958280405319817E-4	7.114574448386307
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54984	7.052530992066801	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1131	0.14506788433048798	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1013	0.12993259666382345	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTG	941	0.12069750588416374	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	872	0.11184721055365653	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.695908983049761E-4	0.0	0.0	0.05553880982767577	0.0
2	7.695908983049761E-4	0.0	0.0	0.22215523931070308	0.0
3	7.695908983049761E-4	0.0	0.0	0.42494244101406425	0.0
4	7.695908983049761E-4	0.0	0.0	0.625549135172228	0.0
5	7.695908983049761E-4	0.0	0.0	1.1146241510450403	0.0
6	7.695908983049761E-4	0.0	0.0	1.6559030828528734	0.0
7	7.695908983049761E-4	0.0	0.0	2.0828977662624175	0.0
8	7.695908983049761E-4	0.0	0.0	2.668428174722787	0.0
9	7.695908983049761E-4	0.0	0.0	3.0337273211182154	0.0
10	7.695908983049761E-4	0.0	0.0	3.5292155944769026	0.0
11	7.695908983049761E-4	0.0	0.0	4.1010216319175	0.0
12	7.695908983049761E-4	0.0	0.0	4.520063876044559	0.0
13	0.0010261211977399681	0.0	0.0	4.712333335471086	0.0
14	0.0010261211977399681	0.0	0.0	4.847781333572762	0.0
15	0.0010261211977399681	0.0	0.0	4.984383718021895	0.0
16	0.0011543863474574641	0.0	0.0	5.2162871087111276	0.0
17	0.0011543863474574641	0.0	0.0	5.461658340120698	0.0
18	0.0011543863474574641	0.0	0.0	5.746150442194104	0.0
19	0.0011543863474574641	0.0	0.0	5.932519704733625	0.0
20	0.0011543863474574641	0.0	0.0	6.136974353383314	0.0
21	0.0011543863474574641	0.0	0.0	6.381960789343731	0.0
22	0.0011543863474574641	0.0	0.0	6.632719157041436	0.0
23	0.0011543863474574641	0.0	0.0	6.877962123301288	0.0
24	0.0011543863474574641	0.0	0.0	7.086649521891655	0.0
25	0.0011543863474574641	0.0	0.0	7.285203973654339	0.0
26	0.0011543863474574641	0.0	0.0	7.47670384218256	0.0
27	0.0011543863474574641	0.0	0.0	7.7119421267644475	0.0
28	0.0011543863474574641	0.0	0.0	7.905622502837867	0.0
29	0.0011543863474574641	0.0	0.0	8.116362143823713	0.0
30	0.0011543863474574641	0.0	0.0	8.390721299069437	0.0
31	0.0012826514971749602	0.0	0.0	8.598767371911215	0.0
32	0.0012826514971749602	0.0	0.0	8.809378747747344	0.0
33	0.0014109166468924562	0.0	0.0	9.024735934123019	0.0
34	0.0014109166468924562	0.0	0.0	9.240990976546717	0.0
35	0.0014109166468924562	0.0	0.0	9.48315557921335	0.0
36	0.0014109166468924562	0.0	0.0	9.706593470021227	0.0
37	0.0014109166468924562	0.0	0.0	9.941446959153962	0.0
38	0.0014109166468924562	0.0	0.0	10.25505525021324	0.0
39	0.0014109166468924562	0.0	0.0	10.720529478538033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	25	3.889647E-5	45.0	1
CGAATCC	20	7.031889E-4	45.0	19
GCGATTC	20	7.031889E-4	45.0	9
CACGTCA	25	3.889647E-5	45.0	34
ACGAAAC	20	7.031889E-4	45.0	29
CGTTTTT	30350	0.0	44.525536	1
GTTTTTT	33725	0.0	40.249817	2
TAGACGG	40	3.4563163E-7	39.375	2
GTCGAAG	30	1.139585E-4	37.499996	1
CCATACG	30	1.139585E-4	37.499996	2
ACAACGA	115	0.0	37.173916	13
GCTACGA	85	0.0	37.058823	10
TGACGGC	25	0.0021066936	36.0	14
TCGATAG	25	0.0021066936	36.0	1
GCTTCGA	25	0.0021066936	36.0	24
ACGCTGG	25	0.0021066936	36.0	2
ACTCGCT	25	0.0021066936	36.0	41
GTGCCGT	50	4.8754373E-8	36.0	42
GGCGATT	195	0.0	35.76923	8
AGGGATG	485	0.0	35.72165	6
>>END_MODULE