##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935685.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301881
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31720114879704	33.0	31.0	34.0	30.0	34.0
2	32.52157307018329	34.0	31.0	34.0	31.0	34.0
3	32.381077311920926	34.0	31.0	34.0	30.0	34.0
4	36.02363182843571	37.0	35.0	37.0	35.0	37.0
5	36.05655208509313	37.0	35.0	37.0	35.0	37.0
6	36.07005740672649	37.0	35.0	37.0	35.0	37.0
7	36.217956744545035	37.0	37.0	37.0	35.0	37.0
8	35.95384936448468	37.0	37.0	37.0	35.0	37.0
9	37.78719429179047	39.0	38.0	39.0	35.0	39.0
10	37.66960159798066	39.0	38.0	39.0	35.0	39.0
11	37.638503251281136	39.0	37.0	39.0	35.0	39.0
12	37.57072488828379	39.0	37.0	39.0	35.0	39.0
13	37.536327228278694	39.0	37.0	39.0	35.0	39.0
14	38.925775388315266	41.0	38.0	41.0	35.0	41.0
15	39.02883917835173	41.0	39.0	41.0	35.0	41.0
16	39.08568608160169	41.0	39.0	41.0	35.0	41.0
17	39.037776474836114	41.0	38.0	41.0	35.0	41.0
18	38.41928773258337	39.0	38.0	40.0	35.0	41.0
19	37.735501737439584	37.0	37.0	40.0	35.0	41.0
20	36.92586151496782	36.0	35.0	40.0	34.0	41.0
21	36.89690639689149	36.0	35.0	40.0	34.0	41.0
22	36.89389858917918	36.0	35.0	40.0	34.0	41.0
23	36.902286000112625	36.0	35.0	40.0	34.0	41.0
24	36.737535651465315	36.0	35.0	40.0	34.0	41.0
25	36.66048873562762	35.0	35.0	40.0	33.0	41.0
26	36.51514338431369	35.0	35.0	40.0	33.0	41.0
27	36.40591822605596	35.0	35.0	40.0	33.0	41.0
28	36.26615785690388	35.0	35.0	40.0	33.0	41.0
29	36.05755910441532	35.0	35.0	40.0	33.0	41.0
30	35.57176834580513	35.0	35.0	40.0	31.0	41.0
31	34.94780062342446	35.0	35.0	40.0	25.0	41.0
32	33.95529364219676	35.0	34.0	40.0	18.0	41.0
33	32.845866417561886	35.0	33.0	40.0	12.0	41.0
34	31.92338040486152	35.0	31.0	40.0	9.0	41.0
35	31.379149399929112	35.0	27.0	40.0	7.0	41.0
36	31.02890542962293	35.0	24.0	40.0	7.0	41.0
37	30.835726660505298	35.0	23.0	40.0	7.0	41.0
38	30.6774457484903	35.0	23.0	40.0	7.0	41.0
39	30.55089588281475	35.0	23.0	40.0	7.0	41.0
40	30.415120527625124	35.0	23.0	40.0	7.0	41.0
41	30.24201920624352	35.0	22.0	40.0	7.0	41.0
42	30.1071415557786	35.0	21.0	40.0	7.0	41.0
43	29.953272978425275	35.0	21.0	40.0	7.0	41.0
44	29.80055054806364	35.0	20.0	40.0	7.0	41.0
45	29.742802627525414	35.0	20.0	40.0	7.0	41.0
46	29.684441882728624	35.0	20.0	40.0	7.0	41.0
47	29.67279822181588	35.0	20.0	40.0	7.0	41.0
48	29.634021352784707	35.0	20.0	40.0	7.0	41.0
49	29.604884043712588	35.0	20.0	40.0	7.0	41.0
50	29.489272262911545	35.0	20.0	39.0	7.0	41.0
51	28.86159778190744	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	7.0
11	9.0
12	5.0
13	2.0
14	11.0
15	24.0
16	21.0
17	61.0
18	148.0
19	288.0
20	504.0
21	790.0
22	1339.0
23	2192.0
24	4234.0
25	8497.0
26	14871.0
27	18699.0
28	16889.0
29	13220.0
30	10164.0
31	8569.0
32	8443.0
33	9014.0
34	14262.0
35	22582.0
36	21707.0
37	20570.0
38	37593.0
39	67089.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.01826216290525	13.353937478675373	14.879704254325379	44.748096104093996
2	56.96781181988929	16.25176808080005	15.026119563669127	11.754300535641528
3	19.575263100360736	17.113365862707493	52.2586714632587	11.052699573673069
4	18.909769081194245	15.595880495957015	52.99439182989324	12.499958592955503
5	16.689026470695406	18.337689354414486	51.282127725825745	13.691156449064367
6	16.37897052149688	22.446593194006912	51.3165783868478	9.857857897648412
7	49.526469039124684	4.24273140740888	42.748632739390686	3.482166814075745
8	48.84341843309119	4.620032396871615	42.08711379649597	4.44943537354123
9	45.034964108373835	5.3004329520572675	43.53106025221859	6.133542687350314
10	25.78002590424704	16.785090813929994	48.00103351983066	9.433849761992308
11	19.87670638430375	16.003325813814055	51.598477545787915	12.521490256094289
12	18.332389252718787	13.942580023254198	54.12894484912929	13.596085874897726
13	15.383545171772983	16.17193529900855	55.695456156565	12.749063372653463
14	12.27304798910829	19.467604784666808	53.958679082154895	14.300668144070015
15	11.698980724192646	16.613831277887645	58.6320437523395	13.05514424558021
16	12.458551548457836	16.574411771525867	56.6236364660247	14.343400213991606
17	12.324723980641378	16.345182373186788	54.01234261182386	17.31775103434797
18	13.865066035954566	16.708570595698305	54.87460290644327	14.551760461903863
19	14.690225618704059	17.356176771641806	53.44788178123168	14.505715828422458
20	16.53565477787605	16.668157320268584	53.335917132909984	13.460270768945378
21	15.071832940794552	17.68842689669108	53.529039588447105	13.710700574067264
22	13.690162679996423	16.245805466392387	53.064618177361275	16.999413676249915
23	13.518571887598094	17.57116214667369	53.66286715626356	15.247398809464658
24	14.673994057260975	16.05003296000742	53.79437592958814	15.481597053143457
25	13.430457696907059	18.186636456087	52.719780310784714	15.663125536221226
26	13.870366137650267	17.846104922138192	53.44721926851972	14.836309671691824
27	14.779664834819018	18.657351737936473	52.470675531086755	14.092307896157758
28	11.94907927295855	19.332120935070442	53.90899062875769	14.80980916321332
29	14.504390802998532	18.10713493065148	52.107618564931215	15.280855701418773
30	14.043944468184483	18.623563589626375	51.31591587413583	16.016576068053308
31	14.903885968312016	20.54153789075828	48.1795806957046	16.374995445225103
32	15.589586625193371	21.692984984149383	46.70283986073983	16.014588529917418
33	14.514328493677972	22.694372948280943	44.056101576448995	18.735196981592082
34	15.058251430199318	22.678803899549823	42.925523633484715	19.33742103676614
35	15.793971796833853	23.804081740818404	39.650392041897305	20.751554420450443
36	16.82384780757981	26.720131442522053	38.746724702780234	17.709296047117903
37	16.034132654920317	27.820565057092033	39.011729787565294	17.13357250042235
38	15.518366508657385	27.559866304934726	37.32563493562033	19.596132250787562
39	17.61422547295126	24.87635856512997	38.27037806287908	19.239037899039687
40	17.338951441130774	23.382061143298188	39.29859779184513	19.980389623725905
41	15.751239726912262	25.44280693385804	37.427661893262574	21.378291445967122
42	17.223342972893292	26.352768143738757	37.06162361990321	19.362265263464742
43	17.85273004925782	26.408750467899605	37.2302331050977	18.508286377744874
44	16.433959076589783	26.10432587675276	37.299796939853785	20.161918106803675
45	16.835110523683174	23.757705850981015	37.46675014326837	21.940433482067437
46	17.854386331037727	23.93592177049897	37.9470718594413	20.262620039021996
47	15.560767322222995	24.034304908225426	40.79422023910084	19.610707530450743
48	15.288805853962323	23.19357627674481	40.334436416998756	21.183181452294118
49	15.218910762850262	22.377691871962792	42.063594595221296	20.339802769965647
50	14.963843368744637	21.656546784991438	41.57267267565696	21.80693717060696
51	14.641530934374803	21.35344721926852	39.80475750378461	24.200264342572073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	106.0
1	181.0
2	256.0
3	18484.5
4	36713.0
5	24143.5
6	11574.0
7	11094.0
8	10614.0
9	10222.5
10	9831.0
11	9318.0
12	8805.0
13	8100.0
14	7395.0
15	6715.5
16	6036.0
17	5520.5
18	5005.0
19	4647.0
20	4289.0
21	3871.5
22	3454.0
23	3114.0
24	2774.0
25	2758.5
26	2649.0
27	2555.0
28	2662.0
29	2769.0
30	3083.0
31	3397.0
32	3669.5
33	3942.0
34	4078.5
35	4215.0
36	4556.5
37	4898.0
38	5770.0
39	6642.0
40	7317.5
41	7993.0
42	9138.5
43	10284.0
44	11802.5
45	13321.0
46	16900.5
47	20480.0
48	21630.0
49	22780.0
50	22654.5
51	22529.0
52	18773.0
53	15017.0
54	13191.0
55	11365.0
56	10069.5
57	8774.0
58	8219.5
59	7665.0
60	7709.0
61	7753.0
62	6754.0
63	5755.0
64	4803.0
65	3851.0
66	3058.0
67	2265.0
68	1963.5
69	1662.0
70	1296.5
71	931.0
72	769.0
73	607.0
74	478.0
75	314.0
76	279.0
77	214.0
78	149.0
79	92.0
80	35.0
81	25.5
82	16.0
83	10.0
84	4.0
85	3.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	301881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.00922320801405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.99874363450901	35.206825649718
2	8.721928579283759	7.6769060330011145
3	3.0060634246604176	3.9688354869998235
4	1.4527713732467633	2.5574135854171955
5	0.897438725991454	1.9747790603836828
6	0.6680693471949259	1.7640727811480235
7	0.49666246264296887	1.5300410424247446
8	0.4519334829861187	1.5911393222329053
9	0.4133101046465325	1.63705109845652
>10	3.7636376691445177	27.985006843724
>50	0.09764862131267744	2.933252631576416
>100	0.026493811984059774	2.164044190201067
>500	0.00378483028343711	1.1959542245723263
>1k	7.56966056687422E-4	0.3504578953342861
>5k	0.0	0.0
>10k+	7.56966056687422E-4	7.4642201548099
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22406	7.422129912117689	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1052	0.34848168649235955	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	863	0.28587423521188815	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	771	0.2553986504616057	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	680	0.22525432206730467	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	654	0.2166416568117901	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	622	0.20604145342038752	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	437	0.1447590275638414	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	399	0.13217128603655082	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	336	0.11130213560972702	No Hit
CGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	333	0.11030836654178301	No Hit
TCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	323	0.10699580298196973	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.937690679439912E-4	0.0	0.0	0.23452950003478193	0.0
2	9.937690679439912E-4	0.0	0.0	1.097783563722129	0.0
3	9.937690679439912E-4	0.0	0.0	1.5370294917533731	0.0
4	9.937690679439912E-4	0.0	0.0	2.0511393562363978	0.0
5	9.937690679439912E-4	0.0	0.0	3.5325177801849073	0.0
6	9.937690679439912E-4	0.0	0.0	4.22053723155813	0.0
7	9.937690679439912E-4	0.0	0.0	4.9284320642902335	0.0
8	9.937690679439912E-4	0.0	0.0	6.024559346232456	0.0
9	9.937690679439912E-4	0.0	0.0	6.415773102646407	0.0
10	9.937690679439912E-4	0.0	0.0	7.4757934417866645	0.0
11	9.937690679439912E-4	0.0	0.0	8.722642365700391	0.0
12	9.937690679439912E-4	0.0	0.0	10.0142108976716	0.0
13	9.937690679439912E-4	0.0	0.0	10.456106876550693	0.0
14	9.937690679439912E-4	0.0	0.0	10.62935395072893	0.0
15	9.937690679439912E-4	0.0	0.0	10.996717249512225	0.0
16	9.937690679439912E-4	0.0	0.0	11.654592372491146	0.0
17	9.937690679439912E-4	0.0	0.0	12.38501263742998	0.0
18	9.937690679439912E-4	0.0	0.0	13.270792133324058	0.0
19	9.937690679439912E-4	0.0	0.0	13.78655827958699	0.0
20	9.937690679439912E-4	0.0	0.0	14.239385718213468	0.0
21	9.937690679439912E-4	0.0	0.0	14.829353288216218	0.0
22	9.937690679439912E-4	0.0	0.0	15.45741533915682	0.0
23	9.937690679439912E-4	0.0	0.0	16.040757782039943	0.0
24	9.937690679439912E-4	0.0	0.0	16.49557275880231	0.0
25	9.937690679439912E-4	0.0	0.0	16.86956118470523	0.0
26	9.937690679439912E-4	0.0	0.0	17.24553714874404	0.0
27	9.937690679439912E-4	0.0	0.0	17.609919140323505	0.0
28	9.937690679439912E-4	0.0	0.0	18.01206435648484	0.0
29	9.937690679439912E-4	0.0	0.0	18.39797801120309	0.0
30	9.937690679439912E-4	0.0	0.0	18.825961223130967	0.0
31	9.937690679439912E-4	0.0	0.0	19.236387848191836	0.0
32	9.937690679439912E-4	0.0	0.0	19.646814473252707	0.0
33	9.937690679439912E-4	0.0	0.0	20.020140386443664	0.0
34	9.937690679439912E-4	0.0	0.0	20.376240969123597	0.0
35	9.937690679439912E-4	0.0	0.0	20.769111007317452	0.0
36	9.937690679439912E-4	0.0	0.0	21.15138084211991	0.0
37	9.937690679439912E-4	0.0	0.0	21.524044242598904	0.0
38	9.937690679439912E-4	0.0	0.0	21.90333277019753	0.0
39	9.937690679439912E-4	0.0	0.0	22.27202109440475	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATGG	20	7.025014E-4	45.0	2
ACGCCAT	25	3.883949E-5	45.0	29
ATAGGGT	25	3.883949E-5	45.0	4
CAACGTC	20	7.025014E-4	45.0	19
CGCGGGC	20	7.025014E-4	45.0	4
CGTGCCG	20	7.025014E-4	45.0	42
GGGTCGA	20	7.025014E-4	45.0	8
GATTTAG	20	7.025014E-4	45.0	19
GCCGACA	20	7.025014E-4	45.0	40
ACGTCTA	20	7.025014E-4	45.0	21
TATAGAG	40	6.7866495E-9	45.0	1
GGTCTGC	20	7.025014E-4	45.0	8
GTAAGCG	20	7.025014E-4	45.0	1
ATTGCGG	35	1.2079545E-7	45.0	2
CGCCGGT	25	3.883949E-5	45.0	28
GGTTATG	20	7.025014E-4	45.0	1
CGTCACT	20	7.025014E-4	45.0	35
TATGGGC	45	3.8198777E-10	44.999996	4
CGGGCCA	30	2.1601663E-6	44.999996	6
CGTTTTT	11170	0.0	44.496418	1
>>END_MODULE