##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935684.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 702325 Sequences flagged as poor quality 0 Sequence length 51 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4268251877692 34.0 31.0 34.0 30.0 34.0 2 32.63265582173495 34.0 31.0 34.0 31.0 34.0 3 32.470342078097744 34.0 31.0 34.0 31.0 34.0 4 36.07238386786744 37.0 35.0 37.0 35.0 37.0 5 36.101794753141355 37.0 35.0 37.0 35.0 37.0 6 36.12099099419784 37.0 36.0 37.0 35.0 37.0 7 36.26462677535329 37.0 37.0 37.0 35.0 37.0 8 36.071454098885845 37.0 37.0 37.0 35.0 37.0 9 37.87160645000534 39.0 38.0 39.0 35.0 39.0 10 37.66263766774642 39.0 38.0 39.0 35.0 39.0 11 37.65695796105791 39.0 37.0 39.0 35.0 39.0 12 37.5934759548642 39.0 37.0 39.0 35.0 39.0 13 37.60762609902823 39.0 38.0 39.0 35.0 39.0 14 38.96330331399281 41.0 39.0 41.0 35.0 41.0 15 39.0804798348343 41.0 39.0 41.0 35.0 41.0 16 39.14084932189513 41.0 39.0 41.0 35.0 41.0 17 39.11443847221728 41.0 39.0 41.0 35.0 41.0 18 38.43672943437867 39.0 38.0 40.0 35.0 41.0 19 37.73130957889866 37.0 37.0 40.0 35.0 41.0 20 36.91499804221692 36.0 35.0 40.0 34.0 41.0 21 36.87316982878297 36.0 35.0 40.0 34.0 41.0 22 36.873521517815824 36.0 35.0 40.0 34.0 41.0 23 36.87593493040971 36.0 35.0 40.0 34.0 41.0 24 36.70730502260349 35.0 35.0 40.0 34.0 41.0 25 36.61203289075571 35.0 35.0 40.0 33.0 41.0 26 36.46863631509629 35.0 35.0 40.0 33.0 41.0 27 36.38911472608835 35.0 35.0 40.0 33.0 41.0 28 36.22869896415477 35.0 35.0 40.0 33.0 41.0 29 35.94370270174065 35.0 35.0 40.0 33.0 41.0 30 35.398779767201795 35.0 35.0 40.0 30.0 41.0 31 34.665321610365574 35.0 35.0 40.0 23.0 41.0 32 33.54932687858185 35.0 33.0 40.0 16.0 41.0 33 32.274466949062045 35.0 31.0 40.0 10.0 41.0 34 31.2095183853629 35.0 25.0 40.0 7.0 41.0 35 30.551318833873207 35.0 22.0 40.0 7.0 41.0 36 30.187445982985086 35.0 21.0 40.0 7.0 41.0 37 30.023839390595523 35.0 20.0 40.0 7.0 41.0 38 29.874325988680454 35.0 20.0 40.0 7.0 41.0 39 29.728225536610545 35.0 18.0 40.0 7.0 41.0 40 29.575863026376677 35.0 18.0 40.0 7.0 41.0 41 29.379912433702344 35.0 18.0 40.0 7.0 41.0 42 29.299979354287544 35.0 18.0 40.0 7.0 41.0 43 29.212939878261487 35.0 18.0 40.0 7.0 41.0 44 29.107497241305662 35.0 18.0 40.0 7.0 41.0 45 29.02742604919375 35.0 18.0 40.0 7.0 41.0 46 28.99748407076496 35.0 18.0 40.0 7.0 41.0 47 29.007710105720285 35.0 18.0 40.0 7.0 41.0 48 28.960956821984123 35.0 18.0 40.0 7.0 41.0 49 28.934674118107715 35.0 18.0 40.0 7.0 41.0 50 28.840441391093865 35.0 18.0 40.0 7.0 41.0 51 28.238405296693127 35.0 18.0 39.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 7.0 9 16.0 10 10.0 11 11.0 12 12.0 13 22.0 14 28.0 15 53.0 16 101.0 17 203.0 18 402.0 19 784.0 20 1381.0 21 2092.0 22 3452.0 23 6089.0 24 12407.0 25 25294.0 26 41226.0 27 47274.0 28 41194.0 29 30916.0 30 23174.0 31 19017.0 32 18135.0 33 20583.0 34 32507.0 35 40568.0 36 42867.0 37 45537.0 38 84442.0 39 162356.0 40 162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.698074253372727 13.259103691311003 14.914462677535328 46.12835937778094 2 60.40138112697113 14.36984302139323 14.630263766774643 10.598512084860998 3 20.473427544228098 15.069946249955505 53.75274979532267 10.703876410493718 4 18.047057985975155 16.283629373865377 53.85569358915032 11.813619051009148 5 16.11889082689638 18.16480973908091 53.21496458192432 12.501334852098386 6 16.140106076246752 21.39337201438081 53.022318727085036 9.444203182287403 7 50.050759975794676 3.85690385505286 42.93012494215641 3.1622112269960487 8 50.95219449684974 3.234115260029189 42.64165450468088 3.1720357384401807 9 47.09600256291603 4.57736802762254 44.05923183711245 4.267397572348984 10 25.14177909087673 16.275086320435694 49.06161677286157 9.521517815826007 11 18.889972591036912 16.031751681913644 53.03442138611042 12.043854340939024 12 16.597586587406116 14.86519773609084 55.809062755846654 12.728152920656392 13 16.05424838927847 14.868472573238886 56.90997757448475 12.1673014629979 14 13.321752749795323 16.713772114049764 56.99612002990069 12.968355106254226 15 13.067312141814687 16.4307122770797 57.821948528103086 12.680027053002526 16 15.32951268999395 16.578791869860822 55.731178585412735 12.360516854732495 17 15.631509628733136 16.31609297689816 54.387213896700246 13.66518349766846 18 16.027195386751146 16.013526501263662 55.380628626348205 12.578649485636992 19 15.279108674758838 17.38198127647457 54.214501833196884 13.124408215569716 20 15.61641690100737 17.404193215391732 54.387356280924074 12.592033602676821 21 15.408251165770833 16.99241804008116 55.7187911579397 11.880539636208308 22 14.810095041469404 15.434592247179014 55.74385078133343 14.011461930018154 23 14.020004983447834 16.48424874523903 55.66012885772257 13.835617413590573 24 14.059445413448191 16.508026910618305 55.41593991385755 14.016587762075963 25 14.569608087423912 17.088100238493574 54.22503826576015 14.117253408322359 26 13.772541202434772 17.226924856725876 54.04221692236501 14.958317018474354 27 13.79247499377069 16.658242266756844 55.23610863916279 14.313174100309686 28 13.028298864485816 17.19389171679778 55.09771117360196 14.680098245114442 29 13.873491617128822 17.055636635460793 54.2939522300929 14.77691951731748 30 15.272274232015093 16.31310290819777 54.05161428113765 14.363008578649486 31 15.17502580714057 17.695511337343824 52.74780194354466 14.381660911970954 32 15.950165521660201 18.788025486776068 50.20368063218596 15.05812835937778 33 16.878225892571102 19.52956252447229 47.77275477877051 15.819456804186096 34 16.30470223899192 21.524365500302565 45.86366710568469 16.307265155020822 35 17.03812337592995 23.355711387178303 42.41127683052718 17.194888406364576 36 18.156693838322713 24.075178870181187 41.05407040899868 16.71405688249742 37 17.743637204997686 25.129249279179867 40.65923895632364 16.467874559498807 38 18.6081942120813 24.71761648809312 39.646744740682735 17.027444559142847 39 18.952621649521234 24.0818709287011 39.13074431353006 17.834763108247607 40 19.613853984978462 23.456377033424697 39.906311180721175 17.02345780087566 41 17.577759584238066 24.32961947816182 38.99861175381768 19.094009183782436 42 17.90581283593778 24.835937778094184 39.075784003132455 18.182465382835584 43 18.11155803936924 24.45278183177304 39.42661873064465 18.00904139821308 44 18.717118143309722 23.74584416046702 38.90050902360018 18.636528672623072 45 18.320435695724914 23.002883280532515 39.015128323781724 19.661552699960843 46 18.31929662193429 22.89182358594668 39.663830847542094 19.125048944576942 47 16.795643042750864 23.201651656996404 41.51069661481508 18.492008685437654 48 16.84505036841918 23.286370270174064 41.17979567863881 18.688783682767948 49 17.860249884312818 22.469084825401346 41.24415334780907 18.426511942476772 50 16.87124906560353 22.277578044352687 41.69921332716335 19.15195956288043 51 16.873669597408608 22.41469405189905 40.24262271740291 20.469013633289432 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 154.0 1 362.5 2 571.0 3 38035.5 4 75500.0 5 50956.5 6 26413.0 7 25697.0 8 24981.0 9 24419.5 10 23858.0 11 23125.0 12 22392.0 13 21400.0 14 20408.0 15 18902.0 16 17396.0 17 16012.5 18 14629.0 19 13178.0 20 11727.0 21 10829.5 22 9932.0 23 9186.0 24 8440.0 25 7907.0 26 7363.5 27 7353.0 28 7827.0 29 8301.0 30 8852.5 31 9404.0 32 10230.5 33 11057.0 34 12127.5 35 13198.0 36 14530.0 37 15862.0 38 16787.0 39 17712.0 40 18836.5 41 19961.0 42 21989.5 43 24018.0 44 26573.0 45 29128.0 46 33612.5 47 38097.0 48 40797.0 49 43497.0 50 43023.0 51 42549.0 52 38369.0 53 34189.0 54 30472.5 55 26756.0 56 24618.5 57 22481.0 58 21074.0 59 19667.0 60 17790.0 61 15913.0 62 13987.0 63 12061.0 64 10622.5 65 9184.0 66 7764.5 67 6345.0 68 5505.0 69 4665.0 70 3877.0 71 3089.0 72 2434.0 73 1779.0 74 1499.5 75 893.5 76 567.0 77 446.5 78 326.0 79 214.0 80 102.0 81 74.0 82 46.0 83 29.0 84 12.0 85 7.0 86 2.0 87 2.5 88 3.0 89 3.5 90 4.0 91 3.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 702325.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.94153548427746 #Duplication Level Percentage of deduplicated Percentage of total 1 80.81595756904365 37.93625139923124 2 7.340184440240573 6.891190567253883 3 2.7926837826898474 3.9327859464450508 4 1.5976520128739784 2.9998495461540466 5 1.1412295983128058 2.678553484245414 6 0.8979166558836545 2.528975193845179 7 0.7451334205354302 2.4484394830409455 8 0.633076325355563 2.3774059832747314 9 0.5862312674193817 2.476673625740387 >10 3.3515730495171043 23.577455048821967 >50 0.05376302111798192 1.7449506670732842 >100 0.04093319752976478 3.113350346093455 >500 0.002749244610207018 0.87297701303483 >1k 6.109432467126707E-4 0.3113063559951734 >5k 0.0 0.0 >10k+ 3.0547162335633533E-4 6.109835339750504 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42609 6.066849393087246 No Hit CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG 1169 0.16644715765493184 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 1002 0.14266899227565585 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 857 0.12202327982059587 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 747 0.10636101519951588 No Hit CGTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTT 744 0.1059338625280319 No Hit CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC 729 0.10379809917061189 No Hit GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC 709 0.1009504146940519 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.695368953119994E-4 0.0 0.0 0.07745701776243193 0.0 2 5.695368953119994E-4 0.0 0.0 0.34115260029188765 0.0 3 5.695368953119994E-4 0.0 0.0 0.6330402591392874 0.0 4 5.695368953119994E-4 0.0 0.0 0.905706047769907 0.0 5 5.695368953119994E-4 0.0 0.0 1.5602463247072225 0.0 6 5.695368953119994E-4 0.0 0.0 2.18830313601253 0.0 7 5.695368953119994E-4 0.0 0.0 2.628697540312533 0.0 8 5.695368953119994E-4 0.0 0.0 3.234400028476845 0.0 9 5.695368953119994E-4 0.0 0.0 3.4999466059160644 0.0 10 5.695368953119994E-4 0.0 0.0 4.0411490406862915 0.0 11 5.695368953119994E-4 0.0 0.0 4.752500622930979 0.0 12 5.695368953119994E-4 0.0 0.0 5.339266009326167 0.0 13 5.695368953119994E-4 0.0 0.0 5.621756309400919 0.0 14 5.695368953119994E-4 0.0 0.0 5.775246502687502 0.0 15 5.695368953119994E-4 0.0 0.0 5.950094329548286 0.0 16 5.695368953119994E-4 0.0 0.0 6.310895952728438 0.0 17 5.695368953119994E-4 0.0 0.0 6.72366781760581 0.0 18 7.119211191399993E-4 0.0 0.0 7.179866870750721 0.0 19 7.119211191399993E-4 0.0 0.0 7.469333997793044 0.0 20 7.119211191399993E-4 0.0 0.0 7.77360908411348 0.0 21 7.119211191399993E-4 0.0 0.0 8.133414017726835 0.0 22 8.543053429679991E-4 0.0 0.0 8.526679243939771 0.0 23 8.543053429679991E-4 0.0 0.0 8.915245790766383 0.0 24 9.96689566795999E-4 0.0 0.0 9.224219556473143 0.0 25 0.0011390737906239988 0.0 0.0 9.515110525753746 0.0 26 0.0011390737906239988 0.0 0.0 9.789342540846475 0.0 27 0.0012814580144519987 0.0 0.0 10.080660662798563 0.0 28 0.0014238422382799986 0.0 0.0 10.365001957783077 0.0 29 0.0014238422382799986 0.0 0.0 10.667283664969922 0.0 30 0.0015662264621079983 0.0 0.0 11.056989285587157 0.0 31 0.0015662264621079983 0.0 0.0 11.385042537286868 0.0 32 0.0017086106859359983 0.0 0.0 11.711529562524472 0.0 33 0.0018509949097639982 0.0 0.0 12.045990104296443 0.0 34 0.0018509949097639982 0.0 0.0 12.347559890364147 0.0 35 0.0018509949097639982 0.0 0.0 12.66906346776777 0.0 36 0.0018509949097639982 0.0 0.0 12.973623322535863 0.0 37 0.0018509949097639982 0.0 0.0 13.293703057701206 0.0 38 0.0018509949097639982 0.0 0.0 13.647812622361442 0.0 39 0.0018509949097639982 0.0 0.0 14.14772363222155 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATTC 20 7.03141E-4 45.000004 27 CTAACGG 20 7.03141E-4 45.000004 2 GCGATAT 20 7.03141E-4 45.000004 9 AACCCGC 25 3.889252E-5 44.999996 21 CGTTTTT 26055 0.0 44.576855 1 CACGAGG 45 1.9264917E-8 40.0 2 GTTTTTT 29185 0.0 39.91177 2 ATTTGCG 35 6.244818E-6 38.57143 1 AGCCGCA 30 1.1394701E-4 37.499996 28 CCATACG 30 1.1394701E-4 37.499996 2 CTTGACG 30 1.1394701E-4 37.499996 1 CGGTCTA 30 1.1394701E-4 37.499996 31 ATTCTCG 30 1.1394701E-4 37.499996 30 CCCGCAT 30 1.1394701E-4 37.499996 23 TTCTCGT 30 1.1394701E-4 37.499996 31 ATTAGCG 25 0.0021065522 35.999996 1 CGACGGT 25 0.0021065522 35.999996 28 CAACGAC 25 0.0021065522 35.999996 12 TAACGCC 25 0.0021065522 35.999996 12 TAACGAG 50 4.874346E-8 35.999996 1 >>END_MODULE