##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935682.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792651
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4652892634968	34.0	31.0	34.0	31.0	34.0
2	32.66018714415298	34.0	31.0	34.0	31.0	34.0
3	32.505364908389694	34.0	31.0	34.0	31.0	34.0
4	36.09870169847764	37.0	35.0	37.0	35.0	37.0
5	36.13130242691929	37.0	35.0	37.0	35.0	37.0
6	36.147136633903195	37.0	37.0	37.0	35.0	37.0
7	36.2746378923385	37.0	37.0	37.0	35.0	37.0
8	36.07262464817429	37.0	37.0	37.0	35.0	37.0
9	37.878011886694146	39.0	38.0	39.0	35.0	39.0
10	37.72266230661413	39.0	38.0	39.0	35.0	39.0
11	37.68890596239707	39.0	38.0	39.0	35.0	39.0
12	37.59326866426712	39.0	37.0	39.0	35.0	39.0
13	37.581032509894015	39.0	37.0	39.0	35.0	39.0
14	38.899684728840306	41.0	39.0	41.0	35.0	41.0
15	39.00749257870109	41.0	39.0	41.0	35.0	41.0
16	39.094605318103426	41.0	39.0	41.0	35.0	41.0
17	39.078530147568095	41.0	39.0	41.0	35.0	41.0
18	38.42932639963868	39.0	38.0	41.0	35.0	41.0
19	37.73946667575011	37.0	37.0	41.0	35.0	41.0
20	36.92286643175874	36.0	35.0	40.0	34.0	41.0
21	36.85873858734803	36.0	35.0	40.0	34.0	41.0
22	36.86897890748892	36.0	35.0	40.0	34.0	41.0
23	36.89298316661431	36.0	35.0	40.0	34.0	41.0
24	36.726889892272894	35.0	35.0	40.0	34.0	41.0
25	36.641466420909076	35.0	35.0	40.0	33.0	41.0
26	36.48829055914898	35.0	35.0	40.0	33.0	41.0
27	36.40164839254603	35.0	35.0	40.0	33.0	41.0
28	36.25180817282764	35.0	35.0	40.0	33.0	41.0
29	35.98187979325075	35.0	35.0	40.0	33.0	41.0
30	35.45632188693384	35.0	35.0	40.0	31.0	41.0
31	34.7993454874844	35.0	35.0	40.0	24.0	41.0
32	33.79816211674495	35.0	34.0	40.0	18.0	41.0
33	32.687202816876535	35.0	33.0	40.0	12.0	41.0
34	31.713431257892818	35.0	30.0	40.0	8.0	41.0
35	31.135847933075212	35.0	25.0	40.0	7.0	41.0
36	30.785884329925782	35.0	23.0	40.0	7.0	41.0
37	30.606421994042776	35.0	23.0	40.0	7.0	41.0
38	30.41329033837086	35.0	22.0	40.0	7.0	41.0
39	30.257936973523027	35.0	22.0	40.0	7.0	41.0
40	30.11724201445529	35.0	21.0	40.0	7.0	41.0
41	29.930620159439652	35.0	21.0	40.0	7.0	41.0
42	29.82936752744903	35.0	20.0	40.0	7.0	41.0
43	29.72813886565462	35.0	20.0	40.0	7.0	41.0
44	29.627539736908172	35.0	20.0	40.0	7.0	41.0
45	29.57816617906241	35.0	20.0	40.0	7.0	41.0
46	29.557573257335196	35.0	20.0	40.0	7.0	41.0
47	29.585638572335114	35.0	20.0	40.0	7.0	41.0
48	29.54309904358917	35.0	20.0	40.0	7.0	41.0
49	29.522165492757846	35.0	20.0	40.0	7.0	41.0
50	29.424295181612084	35.0	20.0	40.0	7.0	41.0
51	28.833134633022603	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	15.0
10	16.0
11	27.0
12	24.0
13	19.0
14	33.0
15	59.0
16	100.0
17	228.0
18	423.0
19	724.0
20	1287.0
21	2026.0
22	3424.0
23	5864.0
24	11763.0
25	24042.0
26	41177.0
27	50212.0
28	45128.0
29	34550.0
30	25936.0
31	21855.0
32	20975.0
33	24913.0
34	40520.0
35	52746.0
36	55671.0
37	53440.0
38	95406.0
39	179854.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.786063475602756	12.87678940668718	14.600372673471679	45.736774444238385
2	58.759908206764386	14.78885411107789	14.99903488420503	11.452202797952694
3	20.811302830627856	15.25564214263276	52.95508363706095	10.977971389678434
4	18.860633494438282	15.805316589520482	52.80798232765744	12.52606758838379
5	16.023697692931695	18.8275798554471	52.420674420394356	12.728048031226857
6	16.934565149100926	21.643699433924894	51.67671522523784	9.745020191736337
7	50.63754413985474	4.084142958250226	41.881609939304944	3.3967029625900933
8	51.33066128725	3.5159231490277563	41.79784041147996	3.355575152242286
9	47.6440451093861	4.873519367287747	43.221543907722314	4.260891615603841
10	27.321229645834045	15.247946448058478	47.689714641122	9.741109264985473
11	20.11238237257002	16.119073842081825	51.08843614655125	12.680107638796898
12	18.429800757205882	14.403186269871608	54.144510005033744	13.02250296788877
13	16.825816153641387	14.432833617821714	55.47271119319852	13.268639035338378
14	13.873066456738211	16.28093574599666	55.824063806139144	14.021933991125982
15	13.207830432308798	16.127021854511	57.474348736076784	13.190798977103416
16	15.819951025104364	15.748545072169215	54.96012747098029	13.471376431746126
17	15.956707302457199	15.739966265102801	53.48734815196096	14.815978280479051
18	16.364579114894195	15.757628514945418	54.38282421898162	13.494968151178766
19	16.58283405937796	16.495153604802113	53.422250145398166	13.499762190421762
20	16.69120457805516	16.96534792739806	53.36787564766839	12.975571846878386
21	16.313484749278057	16.24876521949761	54.90739303930734	12.530356991916996
22	15.274313663894956	15.120652090264189	54.592626515326415	15.012407730514438
23	14.996133228873742	16.252045351611237	54.50835235179164	14.243469067723375
24	14.764126961298226	16.345024481139873	54.086855375190346	14.803993182371562
25	15.569147077339206	16.238041710664593	53.248781620158184	14.944029591838023
26	14.33859289901861	16.295191704798203	53.7429461389691	15.623269257214083
27	14.561894200600264	16.420972155463122	54.484255996649225	14.532877647287393
28	13.52423702234653	16.577661543352622	54.15813516919804	15.739966265102801
29	14.7237561045151	17.039277058882156	53.404209418773206	14.832757417829537
30	15.966169222015742	16.17306986302925	52.84078364879373	15.019977266161275
31	15.94232518472821	17.385457155797443	50.76244147802753	15.909776181446816
32	16.53262280625395	18.73081595809505	49.442314461219375	15.294246774431622
33	17.19117240752866	19.316571858232688	46.53271111750316	16.959544616735485
34	15.930087768765825	20.6480531785111	45.602667504361946	17.819191548361133
35	16.860131381907042	22.74330064555523	43.19946609541904	17.19710187711868
36	18.800203368191045	23.30924959408365	40.797652434678064	17.09289460304724
37	18.23450673751752	24.288369030001856	39.90154557302016	17.57557865946047
38	17.990010736124727	24.18226937201871	40.05886575554689	17.768854136309674
39	17.537604822298842	23.17930589881297	39.720885988915676	19.56220328997251
40	18.862904355132333	22.805749314641627	40.31105745151397	18.02028887871207
41	17.331587293777464	23.645084658948264	39.83304127541629	19.19028677185798
42	17.804430953849803	23.97007005605241	39.98834291510388	18.237156074993912
43	18.04779152489557	23.145747624111998	39.97850251876299	18.827958332229443
44	18.337830899096826	23.11118007799145	39.245771468149286	19.305217554762436
45	18.018648812655254	21.990888802259757	39.474245285756275	20.51621709932871
46	17.613678655549542	22.30641227980536	40.303235598012236	19.776673466632854
47	16.682625770988746	22.578410927381658	42.0077688667522	18.731194434877395
48	16.550789691806354	22.55355761867455	41.76768842782006	19.12796426169903
49	17.67499189428891	21.50833090477398	42.27144102511698	18.54523617582013
50	16.10267318151368	21.5996699682458	42.56135424039079	19.73630260984973
51	16.336067197291115	21.421155085907923	41.295223244530064	20.947554472270898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135.0
1	417.0
2	699.0
3	49011.5
4	97324.0
5	63857.5
6	30391.0
7	28929.0
8	27467.0
9	26620.5
10	25774.0
11	24802.5
12	23831.0
13	22198.5
14	20566.0
15	18846.0
16	17126.0
17	15618.5
18	14111.0
19	12836.0
20	11561.0
21	10530.0
22	9499.0
23	8614.0
24	7729.0
25	7352.5
26	7091.5
27	7207.0
28	7297.5
29	7388.0
30	7910.0
31	8432.0
32	9670.0
33	10908.0
34	11473.0
35	12038.0
36	13649.0
37	15260.0
38	16066.5
39	16873.0
40	18173.0
41	19473.0
42	21771.5
43	24070.0
44	27057.0
45	30044.0
46	33859.0
47	37674.0
48	42941.5
49	48209.0
50	48120.5
51	48032.0
52	43999.5
53	39967.0
54	36480.0
55	32993.0
56	30807.0
57	28621.0
58	27676.5
59	26732.0
60	25186.5
61	23641.0
62	21121.5
63	18602.0
64	16636.5
65	14671.0
66	12239.5
67	9808.0
68	8418.5
69	7029.0
70	6024.0
71	5019.0
72	4079.5
73	3140.0
74	2446.5
75	1328.0
76	903.0
77	708.0
78	513.0
79	386.5
80	260.0
81	187.0
82	114.0
83	93.5
84	73.0
85	41.0
86	9.0
87	5.0
88	1.0
89	2.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	792651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.15710678973661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4069282453996	33.093165324306
2	7.483742804584978	6.160184035904538
3	2.6413868793771464	3.26135525602603
4	1.4095261525973808	2.320480735415079
5	0.9439673572585298	1.9425482664357379
6	0.7468564038183263	1.84430692611297
7	0.6178302108683148	1.77996727766429
8	0.5638429676702967	1.8564916186438727
9	0.49711747469940015	1.8413925293922657
>10	4.562870979257712	31.948564924474205
>50	0.06916874439955692	2.008323999748799
>100	0.053970217114586674	3.5552893678513833
>500	0.002171215631045859	0.6818240758955133
>1k	3.1017366157797986E-4	0.14808386594997106
>5k	0.0	0.0
>10k+	3.1017366157797986E-4	7.558021796179343
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59205	7.469239299515171	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1160	0.1463443558388244	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	942	0.1188417096553212	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	845	0.10660429369293674	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	834	0.10521654549101685	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	794	0.10017018839312634	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.046357097890496E-4	0.0	0.0	0.06055628517468596	0.0
2	5.046357097890496E-4	0.0	0.0	0.3329334095333255	0.0
3	5.046357097890496E-4	0.0	0.0	0.5103128615241764	0.0
4	5.046357097890496E-4	0.0	0.0	0.7238999256923917	0.0
5	5.046357097890496E-4	0.0	0.0	1.322019400719863	0.0
6	5.046357097890496E-4	0.0	0.0	1.740362404134985	0.0
7	6.307946372363121E-4	0.0	0.0	2.108241836571202	0.0
8	6.307946372363121E-4	0.0	0.0	2.7192295221982943	0.0
9	6.307946372363121E-4	0.0	0.0	2.979747707376891	0.0
10	6.307946372363121E-4	0.0	0.0	3.481229443979759	0.0
11	6.307946372363121E-4	0.0	0.0	4.189359503741243	0.0
12	6.307946372363121E-4	0.0	0.0	4.786469707349136	0.0
13	6.307946372363121E-4	0.0	0.0	5.040049151518134	0.0
14	6.307946372363121E-4	0.0	0.0	5.151321325526619	0.0
15	6.307946372363121E-4	0.0	0.0	5.3440921666660355	0.0
16	6.307946372363121E-4	0.0	0.0	5.700238818849658	0.0
17	6.307946372363121E-4	0.0	0.0	6.0923407653557495	0.0
18	6.307946372363121E-4	0.0	0.0	6.5437374077620545	0.0
19	6.307946372363121E-4	0.0	0.0	6.836678437294598	0.0
20	6.307946372363121E-4	0.0	0.0	7.122428407962647	0.0
21	6.307946372363121E-4	0.0	0.0	7.481855192259897	0.0
22	6.307946372363121E-4	0.0	0.0	7.854654822866558	0.0
23	6.307946372363121E-4	0.0	0.0	8.200834919781846	0.0
24	6.307946372363121E-4	0.0	0.0	8.485701777957765	0.0
25	6.307946372363121E-4	0.0	0.0	8.77069479506113	0.0
26	6.307946372363121E-4	0.0	0.0	9.009639803646245	0.0
27	6.307946372363121E-4	0.0	0.0	9.281259974440202	0.0
28	6.307946372363121E-4	0.0	0.0	9.54354438460306	0.0
29	6.307946372363121E-4	0.0	0.0	9.826771176722165	0.0
30	6.307946372363121E-4	0.0	0.0	10.160209221965278	0.0
31	6.307946372363121E-4	0.0	0.0	10.433343299888602	0.0
32	7.569535646835745E-4	0.0	0.0	10.703954199262979	0.0
33	7.569535646835745E-4	0.0	0.0	10.977845230750987	0.0
34	8.831124921308369E-4	0.0	0.0	11.274949504889289	0.0
35	8.831124921308369E-4	0.0	0.0	11.58038026823911	0.0
36	8.831124921308369E-4	0.0	0.0	11.854018981872224	0.0
37	8.831124921308369E-4	0.0	0.0	12.143301402508797	0.0
38	8.831124921308369E-4	0.0	0.0	12.459077197909295	0.0
39	8.831124921308369E-4	0.0	0.0	12.994874162777817	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	20	7.0319587E-4	45.000004	1
CGTAGCG	20	7.0319587E-4	45.000004	1
TCGTACA	20	7.0319587E-4	45.000004	34
CGGTCTA	40	6.8102963E-9	45.000004	31
CGTAAGG	50	2.1827873E-11	45.0	2
CGTTTTT	30005	0.0	44.47509	1
GGCGATA	125	0.0	41.399998	8
TAGTAGG	100	0.0	40.5	2
GTTTTTT	33160	0.0	40.38601	2
TTACACG	180	0.0	38.75	34
ATTACGG	35	6.2458366E-6	38.571426	2
ATGACGG	35	6.2458366E-6	38.571426	2
CGATAGC	35	6.2458366E-6	38.571426	10
ATGGGCG	155	0.0	37.741936	5
CCATACG	30	1.1396024E-4	37.499996	2
CCCTCGT	55	2.746674E-9	36.81818	14
GCTACGA	55	2.746674E-9	36.81818	10
TGGGCGA	245	0.0	36.734695	6
TGCGGGT	105	0.0	36.42857	4
TCACGAC	50	4.8756192E-8	36.0	25
>>END_MODULE