##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935680.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 325191 Sequences flagged as poor quality 0 Sequence length 51 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46347838654821 34.0 31.0 34.0 31.0 34.0 2 32.66312105808586 34.0 31.0 34.0 31.0 34.0 3 32.503587122644845 34.0 31.0 34.0 31.0 34.0 4 36.105547201490815 37.0 35.0 37.0 35.0 37.0 5 36.13738387593752 37.0 35.0 37.0 35.0 37.0 6 36.14786694588719 37.0 37.0 37.0 35.0 37.0 7 36.286071877758 37.0 37.0 37.0 35.0 37.0 8 36.05911910231218 37.0 37.0 37.0 35.0 37.0 9 37.874286188732164 39.0 38.0 39.0 35.0 39.0 10 37.73773566919134 39.0 38.0 39.0 35.0 39.0 11 37.70254096823098 39.0 38.0 39.0 35.0 39.0 12 37.58817433446805 39.0 37.0 39.0 35.0 39.0 13 37.584561073338435 39.0 37.0 39.0 35.0 39.0 14 38.98424925659074 41.0 39.0 41.0 35.0 41.0 15 39.08669981641558 41.0 39.0 41.0 35.0 41.0 16 39.121937568997915 41.0 39.0 41.0 35.0 41.0 17 39.062514030216086 41.0 39.0 41.0 35.0 41.0 18 38.363761604718455 39.0 38.0 40.0 35.0 41.0 19 37.6435171945103 37.0 37.0 40.0 35.0 41.0 20 36.81203046824789 35.0 35.0 40.0 34.0 41.0 21 36.76019324028033 35.0 35.0 40.0 34.0 41.0 22 36.75672143448004 35.0 35.0 40.0 34.0 41.0 23 36.76603903552066 35.0 35.0 40.0 34.0 41.0 24 36.61190500352101 35.0 35.0 40.0 34.0 41.0 25 36.527880537899264 35.0 35.0 40.0 33.0 41.0 26 36.390834924705786 35.0 35.0 40.0 33.0 41.0 27 36.314476722910534 35.0 35.0 40.0 33.0 41.0 28 36.172061957434245 35.0 35.0 40.0 33.0 41.0 29 35.89627326709534 35.0 35.0 40.0 33.0 41.0 30 35.33224474232067 35.0 35.0 40.0 31.0 41.0 31 34.61951284014625 35.0 35.0 40.0 23.0 41.0 32 33.52689957594152 35.0 34.0 40.0 17.0 41.0 33 32.32090986527918 35.0 33.0 40.0 12.0 41.0 34 31.347441964876026 35.0 28.0 40.0 8.0 41.0 35 30.760356221420643 35.0 23.0 40.0 7.0 41.0 36 30.41288043027021 35.0 22.0 40.0 7.0 41.0 37 30.238407582005653 35.0 22.0 40.0 7.0 41.0 38 30.08264373860285 35.0 21.0 40.0 7.0 41.0 39 29.941031578364715 35.0 21.0 40.0 7.0 41.0 40 29.837553929844308 35.0 21.0 40.0 7.0 41.0 41 29.667724506520784 35.0 20.0 40.0 7.0 41.0 42 29.528655467094723 35.0 18.0 40.0 7.0 41.0 43 29.40941169958578 35.0 18.0 40.0 7.0 41.0 44 29.28430676125723 35.0 18.0 40.0 7.0 41.0 45 29.215971536727647 35.0 20.0 40.0 7.0 41.0 46 29.195900255542128 35.0 20.0 40.0 7.0 41.0 47 29.228782469379535 35.0 20.0 40.0 7.0 41.0 48 29.188144813355844 35.0 20.0 40.0 7.0 41.0 49 29.180982868529572 35.0 20.0 40.0 7.0 41.0 50 29.059085276037774 35.0 20.0 39.0 7.0 41.0 51 28.465486437201523 34.0 20.0 38.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 6.0 10 5.0 11 8.0 12 4.0 13 7.0 14 8.0 15 15.0 16 38.0 17 98.0 18 200.0 19 314.0 20 564.0 21 841.0 22 1388.0 23 2307.0 24 4622.0 25 9672.0 26 17075.0 27 21413.0 28 19625.0 29 15290.0 30 12007.0 31 10125.0 32 8863.0 33 9622.0 34 15956.0 35 24056.0 36 22835.0 37 20334.0 38 37412.0 39 70391.0 40 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.098815772884244 12.119646607685945 13.749765522416057 48.03177209701375 2 60.10283187419086 14.518544486163515 14.329424861081641 11.049198778563984 3 20.32651580148282 14.752253291142745 54.14663997466105 10.77459093271339 4 17.93407566630073 15.612363195783402 54.318538950954974 12.135022186960894 5 15.813783284285234 18.17823986518692 53.65954162323065 12.34843522729719 6 16.510604537025934 20.441217622873943 53.57774354148792 9.4704342986122 7 48.594210786891395 3.9982656346577854 44.081170758108314 3.3263528203425063 8 48.757499438791356 3.673533400370859 44.07133038737234 3.49763677346544 9 45.24479459763647 4.948783945435144 45.48096349529969 4.325457961628705 10 26.093895587516258 15.333142676150324 49.391588328090265 9.181373408243156 11 19.978105175112475 15.503196582931261 52.65582380816197 11.862874433794293 12 18.770507178857965 13.524359530245302 55.177111297668134 12.528021993228593 13 16.100999105141288 14.718119505152357 55.969568653499024 13.211312736207336 14 12.390564314510549 16.213548345433914 57.00988034724208 14.386006992813455 15 12.476975070035763 15.799022728181283 58.390299854547024 13.333702347235931 16 14.763938731391704 15.063762527253214 57.310319166274596 12.86197957508049 17 14.215645574447016 15.393722458493626 55.48400785999612 14.906624107063235 18 14.8860208308348 15.677248140323687 56.20819764384623 13.22853338499528 19 15.389109784711138 16.54104818399033 54.62451297852646 13.445329052772061 20 16.528747720570372 16.31810228450357 54.872059804853144 12.281090190072911 21 16.0062855368076 16.46878296139807 55.5242918776965 12.000639624097838 22 14.851887044844414 15.109274241907064 55.416047799600854 14.622790913647671 23 14.248549314095408 15.822701120264707 56.07350756939768 13.85524199624221 24 15.16770144315187 14.747025594189262 56.19589718042627 13.889375782232596 25 14.29313849399276 15.492126165853298 55.02458555126064 15.190149788893297 26 13.304181235028029 16.53366790593836 54.79425937372191 15.367891485311711 27 13.414577894222166 16.398362808318804 55.68850306435294 14.498556233106083 28 12.072289823519101 17.1256277080239 56.28200042436598 14.520082044091012 29 13.725472107161638 16.487233656528012 55.332404648345126 14.454889587965228 30 14.685523277089466 15.852837255643607 55.20786245621804 14.253777011048891 31 15.463835099987392 16.69080632612834 52.53466424347537 15.310694330408896 32 16.602242989504628 18.442084805545047 49.92911857954249 15.026553625407837 33 16.974332007958402 19.992558219630922 47.39706818454385 15.636041587866822 34 15.946628289220795 21.596538649593626 45.51263718860608 16.9441958725795 35 16.62991903219954 23.103960441709642 42.915394337481665 17.350726188609155 36 17.18251735134121 25.763935656275848 41.07063233607326 15.982914656309678 37 17.683146212533558 25.991494229545097 40.549400198652485 15.77595935926886 38 18.12657791882309 25.887555313646445 39.40330451949777 16.582562248032694 39 18.35690409636183 24.75376009791169 39.59211663299415 17.297219172732333 40 18.642274847704886 23.658711341949807 40.100740795409465 17.598273014935838 41 16.52905523215587 25.15352515906037 38.93680944429581 19.380610164487948 42 17.144693426324835 25.429978074423953 38.90298316989092 18.522345329360284 43 17.780319873551235 24.36260536115698 39.50324578478494 18.35382898050684 44 16.985709936621863 24.035105522600563 39.83505078553834 19.144133755239228 45 17.817221263811113 22.579345676848376 40.47774999923122 19.12568306010929 46 17.283688662970377 23.36842040523877 40.917184054909264 18.430706876881587 47 16.4303440132107 23.01262950081644 42.3920096189624 18.165016867010465 48 16.239994341786826 22.011986801602752 43.182929416865775 18.565089439744643 49 16.676045770024388 21.172172661605025 44.27705563807117 17.874725930299423 50 15.506271698786254 21.50059503491794 43.781654473832305 19.211478792463506 51 15.339907931031302 21.34284159155696 42.75702587094969 20.56022460646205 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 53.0 1 184.5 2 316.0 3 22365.0 4 44414.0 5 29067.0 6 13720.0 7 12792.0 8 11864.0 9 11553.0 10 11242.0 11 10641.0 12 10040.0 13 9343.5 14 8647.0 15 7974.0 16 7301.0 17 6543.5 18 5786.0 19 5180.5 20 4575.0 21 4146.5 22 3718.0 23 3389.0 24 3060.0 25 2916.0 26 2808.0 27 2844.0 28 2777.5 29 2711.0 30 3119.5 31 3528.0 32 3765.0 33 4002.0 34 4639.0 35 5276.0 36 5569.5 37 5863.0 38 6279.5 39 6696.0 40 7824.5 41 8953.0 42 9720.5 43 10488.0 44 11635.5 45 12783.0 46 14158.0 47 15533.0 48 16873.0 49 18213.0 50 18151.5 51 18090.0 52 16314.0 53 14538.0 54 13277.0 55 12016.0 56 11428.5 57 10841.0 58 10864.0 59 10887.0 60 10447.0 61 10007.0 62 9312.5 63 8618.0 64 7237.0 65 5856.0 66 4651.0 67 3446.0 68 2954.0 69 2462.0 70 2112.0 71 1762.0 72 1380.5 73 999.0 74 796.5 75 472.5 76 351.0 77 267.0 78 183.0 79 127.5 80 72.0 81 61.0 82 50.0 83 30.5 84 11.0 85 9.0 86 7.0 87 4.5 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 325191.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.271730756167585 #Duplication Level Percentage of deduplicated Percentage of total 1 79.88046995067826 36.16327128283242 2 7.1959910731076295 6.515499407710282 3 2.7347014219917214 3.7141399942475353 4 1.6998698306146949 3.0782419716848266 5 1.3376958869740638 3.02799040143613 6 1.0846834824588818 2.9463299144124435 7 0.9497343107729268 3.009728120504444 8 0.8525156243727126 3.0875886249622035 9 0.695521665455034 2.833872261620541 >10 3.4504057408041273 22.473579872305216 >50 0.0876104603153127 2.726505689533915 >100 0.02874718229096198 2.550192274674863 >500 0.0013689134424267608 0.3389927519434143 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 6.844567212133804E-4 7.534067432131788 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24314 7.476836689822289 No Hit GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC 563 0.1731290226359278 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 531 0.16328865189996034 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 472 0.14514546835552028 No Hit CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC 468 0.14391542201352436 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 447 0.1374576787180457 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA 400 0.12300463419959347 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA 350 0.1076290549246443 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT 327 0.10055628845816765 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.150231709979673E-4 0.0 0.0 0.09625112626118189 0.0 2 6.150231709979673E-4 0.0 0.0 0.5283049038872539 0.0 3 6.150231709979673E-4 0.0 0.0 0.7681639405764612 0.0 4 6.150231709979673E-4 0.0 0.0 1.0110980931206583 0.0 5 6.150231709979673E-4 0.0 0.0 1.695618882441396 0.0 6 6.150231709979673E-4 0.0 0.0 2.0640177618691786 0.0 7 6.150231709979673E-4 0.0 0.0 2.4416419888619303 0.0 8 6.150231709979673E-4 0.0 0.0 3.011460956791547 0.0 9 9.22534756496951E-4 0.0 0.0 3.2236439507858456 0.0 10 9.22534756496951E-4 0.0 0.0 3.6787610973243416 0.0 11 9.22534756496951E-4 0.0 0.0 4.38419267445901 0.0 12 9.22534756496951E-4 0.0 0.0 4.98353275459653 0.0 13 9.22534756496951E-4 0.0 0.0 5.23753732421869 0.0 14 9.22534756496951E-4 0.0 0.0 5.334710985236369 0.0 15 0.0012300463419959347 0.0 0.0 5.548124025572664 0.0 16 0.0012300463419959347 0.0 0.0 5.946966551964845 0.0 17 0.0012300463419959347 0.0 0.0 6.396856001549859 0.0 18 0.0012300463419959347 0.0 0.0 6.875036517000778 0.0 19 0.0012300463419959347 0.0 0.0 7.17978049823027 0.0 20 0.0015375579274949183 0.0 0.0 7.494979873366729 0.0 21 0.0015375579274949183 0.0 0.0 7.883982029022944 0.0 22 0.0015375579274949183 0.0 0.0 8.265603906627183 0.0 23 0.0015375579274949183 0.0 0.0 8.636770390324456 0.0 24 0.0015375579274949183 0.0 0.0 8.932289023988979 0.0 25 0.0015375579274949183 0.0 0.0 9.205359311912076 0.0 26 0.0015375579274949183 0.0 0.0 9.45536623092275 0.0 27 0.0015375579274949183 0.0 0.0 9.69737784871045 0.0 28 0.0015375579274949183 0.0 0.0 9.967373020778558 0.0 29 0.0015375579274949183 0.0 0.0 10.239213262359659 0.0 30 0.0015375579274949183 0.0 0.0 10.54457226676015 0.0 31 0.0015375579274949183 0.0 0.0 10.81672001992675 0.0 32 0.0015375579274949183 0.0 0.0 11.121156489570744 0.0 33 0.0015375579274949183 0.0 0.0 11.386538987856367 0.0 34 0.0015375579274949183 0.0 0.0 11.658994252608467 0.0 35 0.0015375579274949183 0.0 0.0 11.96066311798297 0.0 36 0.0015375579274949183 0.0 0.0 12.230965801636577 0.0 37 0.0015375579274949183 0.0 0.0 12.51418397188114 0.0 38 0.0015375579274949183 0.0 0.0 12.816160348841143 0.0 39 0.001845069512993902 0.0 0.0 13.111371470920167 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 55 1.8189894E-12 45.0 5 ATGGATT 20 7.025817E-4 45.0 18 AATGGTC 20 7.025817E-4 45.0 16 TACGGGT 20 7.025817E-4 45.0 4 TAGGGTC 25 3.8846156E-5 45.0 5 ATCCAAT 20 7.025817E-4 45.0 28 GGCGATT 75 0.0 45.0 8 ATCATCC 20 7.025817E-4 45.0 28 TCGCGCG 20 7.025817E-4 45.0 1 CGCTTGG 20 7.025817E-4 45.0 2 CTTACGG 20 7.025817E-4 45.0 2 TACCGGG 20 7.025817E-4 45.0 3 CGTTTTT 12855 0.0 44.597435 1 CGAATAT 55 6.002665E-11 40.909092 14 GTTTTTT 14265 0.0 40.536278 2 ACGGGCT 50 1.0768417E-9 40.5 5 ACGGGAT 50 1.0768417E-9 40.5 5 GCGATTG 50 1.0768417E-9 40.5 9 ATAGGGA 110 0.0 38.863636 4 TACGAGG 35 6.234477E-6 38.571426 2 >>END_MODULE