##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935679.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	869857
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.382662897464755	33.0	31.0	34.0	30.0	34.0
2	32.57963435369262	34.0	31.0	34.0	31.0	34.0
3	32.413156415364824	34.0	31.0	34.0	30.0	34.0
4	36.03700608260898	37.0	35.0	37.0	35.0	37.0
5	36.06842503997783	37.0	35.0	37.0	35.0	37.0
6	36.08138694061208	37.0	36.0	37.0	35.0	37.0
7	36.217508165135186	37.0	37.0	37.0	35.0	37.0
8	36.03482871322528	37.0	37.0	37.0	35.0	37.0
9	37.83872291652536	39.0	38.0	39.0	35.0	39.0
10	37.646290137344415	39.0	38.0	39.0	35.0	39.0
11	37.63582060039754	39.0	37.0	39.0	35.0	39.0
12	37.55351166915941	39.0	37.0	39.0	35.0	39.0
13	37.5393553193226	39.0	37.0	39.0	35.0	39.0
14	38.83844126103486	41.0	38.0	41.0	35.0	41.0
15	38.95468450561414	41.0	39.0	41.0	35.0	41.0
16	39.03623124260654	41.0	39.0	41.0	35.0	41.0
17	38.99947692551764	41.0	38.0	41.0	35.0	41.0
18	38.34247008416326	39.0	38.0	40.0	35.0	41.0
19	37.66076378071338	37.0	37.0	40.0	35.0	41.0
20	36.85208258368905	36.0	35.0	40.0	34.0	41.0
21	36.809869898155675	36.0	35.0	40.0	33.0	41.0
22	36.8132773547836	36.0	35.0	40.0	34.0	41.0
23	36.83495563063814	36.0	35.0	40.0	34.0	41.0
24	36.67759413328857	35.0	35.0	40.0	34.0	41.0
25	36.57251939111831	35.0	35.0	40.0	33.0	41.0
26	36.435908430926006	35.0	35.0	40.0	33.0	41.0
27	36.3518658814035	35.0	35.0	40.0	33.0	41.0
28	36.17156957982749	35.0	35.0	40.0	33.0	41.0
29	35.86968432742393	35.0	35.0	40.0	32.0	41.0
30	35.3595073673029	35.0	35.0	40.0	30.0	41.0
31	34.699359779825876	35.0	35.0	40.0	23.0	41.0
32	33.669927355875735	35.0	33.0	40.0	18.0	41.0
33	32.4724374236225	35.0	33.0	40.0	12.0	41.0
34	31.43366783275872	35.0	29.0	40.0	8.0	41.0
35	30.832501204220925	35.0	23.0	40.0	7.0	41.0
36	30.467976920344377	35.0	22.0	40.0	7.0	41.0
37	30.301189735784156	35.0	22.0	40.0	7.0	41.0
38	30.12930401203876	35.0	21.0	40.0	7.0	41.0
39	29.969800783347146	35.0	21.0	40.0	7.0	41.0
40	29.830523867716188	35.0	20.0	40.0	7.0	41.0
41	29.62062155043875	35.0	18.0	40.0	7.0	41.0
42	29.51498464690173	35.0	18.0	40.0	7.0	41.0
43	29.41953217597835	35.0	18.0	40.0	7.0	41.0
44	29.3203871440938	35.0	18.0	40.0	7.0	41.0
45	29.264973438162823	35.0	20.0	40.0	7.0	41.0
46	29.258982798322023	35.0	20.0	40.0	7.0	41.0
47	29.315765694821103	35.0	20.0	40.0	7.0	41.0
48	29.27119170162452	35.0	20.0	40.0	7.0	41.0
49	29.24033145677968	35.0	20.0	40.0	7.0	41.0
50	29.122908707983036	35.0	20.0	40.0	7.0	41.0
51	28.49951658720916	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	13.0
10	28.0
11	31.0
12	21.0
13	23.0
14	43.0
15	61.0
16	142.0
17	265.0
18	501.0
19	1000.0
20	1613.0
21	2636.0
22	4156.0
23	7128.0
24	13673.0
25	28638.0
26	47216.0
27	56161.0
28	50222.0
29	38397.0
30	28996.0
31	25272.0
32	24428.0
33	27723.0
34	44466.0
35	53642.0
36	57890.0
37	59138.0
38	105248.0
39	190926.0
40	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.48775258462023	13.168716237266583	14.787947904080786	46.5555832740324
2	59.153286114844164	14.747826366862599	15.211810676927357	10.887076841365879
3	19.842111979325338	14.905898325816771	54.3764089959614	10.875580698896485
4	17.732339913342077	16.114142899350124	53.869888958759894	12.28362822854791
5	15.882265705742437	18.383596384233268	53.178625912075205	12.555511997949086
6	16.26324786717817	20.702023436036036	52.75246391073475	10.28226478605104
7	50.089267546274854	3.7338321126346052	42.53480744536171	3.6420928957288377
8	51.28877505153145	3.0530305555970694	42.31500120134689	3.343193191524584
9	47.54597594777073	4.395550073172947	43.687755573617274	4.370718405439055
10	25.301630038040734	17.150750065815416	48.02042174748264	9.527198148661217
11	18.385780651302454	16.455003523567665	52.868919833949725	12.29029599118016
12	17.38343198939596	14.227625920122502	55.2251691944768	13.163772896004748
13	16.343835825888622	14.213255742035761	56.303622319530675	13.139286112544934
14	13.044442937172432	16.162311736296886	56.987182950760875	13.806062375769809
15	12.594828805194416	16.212779801737526	57.99355526253166	13.198836130536398
16	15.741667883341744	16.91105549532854	55.19160045846616	12.155676162863552
17	15.746381301754198	16.816557204230122	53.678478186644476	13.758583307371211
18	16.43971365408337	16.248302881967955	54.331574040330764	12.980409423617905
19	15.713617295716423	17.997211035836923	53.00848300352816	13.280688664918486
20	15.136510943752823	18.295995778616486	53.0648141016282	13.502679176002491
21	15.208016949912457	17.780853634562924	54.946732623868066	12.06439679165656
22	14.86094840876144	15.629005687141678	55.55384390767678	13.9562019964201
23	13.99471407369257	16.343375980189847	55.346798381802984	14.315111564314595
24	14.466975606335295	16.426493090243568	54.67818273578301	14.42834856763813
25	15.457713164347703	17.0200389259384	52.992503365495715	14.529744544218188
26	13.93746328419499	17.026016920022485	53.04906438644513	15.987455409337398
27	14.057138127301382	16.10563575392277	54.00933716691364	15.827888951862205
28	13.689606452554845	16.942554925694683	53.34658455355306	16.021254068197415
29	14.205783249430654	16.920137447879362	53.078724434016166	15.795354868673817
30	15.452539900236475	16.151275439526266	52.460002046313356	15.936182613923897
31	15.248138487130644	17.61209026311221	51.08920201826277	16.05056923149437
32	15.966072584344323	18.45533231324229	49.04737215427363	16.53122294813975
33	16.477650924232375	19.770491011741008	46.18954609780688	17.562311966219735
34	15.592103069814925	22.20732833097854	45.04544999925275	17.155118599953788
35	16.34958389712332	23.82506549927172	42.03909378208142	17.786256821523537
36	18.333013357367935	25.14930615032126	39.45142707364544	17.06625341866537
37	18.020893089323877	25.701465873126274	39.1883953339457	17.089245703604156
38	18.536954924774992	25.073546571447952	38.7648774453732	17.624621058403854
39	18.705258450526927	24.635543543364026	38.04797800098177	18.61122000512728
40	20.26459521507558	23.6792944127598	38.96628986143699	17.089820510727623
41	18.432569951152892	24.697047905575285	38.2050153071137	18.665366836158125
42	18.683070895560995	24.97559943760871	38.451607563082206	17.88972210374809
43	18.419924194436557	24.109939909663314	38.821783350596704	18.648352545303425
44	18.89770387546459	23.762641445662908	38.228007592052485	19.111647086820017
45	18.326920401859155	22.967223348205508	38.38228582399176	20.323570425943576
46	17.925934952526678	23.08183988862537	39.136547731408726	19.855677427439222
47	17.16132651688726	22.94538067751366	40.75325024687966	19.140042558719422
48	16.891627014555265	22.944920831814887	41.075946965995556	19.087505187634292
49	17.62013756284079	21.97441648454861	41.05352948818024	19.35191646443036
50	16.127708347464008	22.00028280510475	42.280627735363396	19.591381112067847
51	16.539730093567105	22.033736579690686	40.93695860354058	20.489574723201628
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	252.0
1	486.5
2	721.0
3	50728.0
4	100735.0
5	66786.5
6	32838.0
7	31235.5
8	29633.0
9	28934.0
10	28235.0
11	27188.0
12	26141.0
13	24599.5
14	23058.0
15	21119.5
16	19181.0
17	17712.0
18	16243.0
19	14800.5
20	13358.0
21	12034.0
22	10710.0
23	10060.0
24	9410.0
25	9284.5
26	9306.5
27	9454.0
28	9553.0
29	9652.0
30	10510.5
31	11369.0
32	12147.5
33	12926.0
34	14681.0
35	16436.0
36	17115.0
37	17794.0
38	19128.5
39	20463.0
40	21815.5
41	23168.0
42	24992.5
43	26817.0
44	29780.5
45	32744.0
46	39229.0
47	45714.0
48	49938.5
49	54163.0
50	53942.5
51	53722.0
52	47848.0
53	41974.0
54	38459.0
55	34944.0
56	32829.5
57	30715.0
58	29047.5
59	27380.0
60	25083.5
61	22787.0
62	20117.0
63	17447.0
64	15570.5
65	13694.0
66	11602.0
67	9510.0
68	8187.5
69	6865.0
70	5509.5
71	4154.0
72	3422.0
73	2690.0
74	2295.0
75	1405.0
76	910.0
77	678.5
78	447.0
79	320.5
80	194.0
81	139.5
82	85.0
83	61.5
84	38.0
85	23.5
86	9.0
87	10.5
88	12.0
89	6.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	869857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.41761062627213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96020783603204	35.861839744664245
2	7.622344149687265	6.923773172998791
3	3.177395381336448	4.329291187057627
4	1.9418546174450133	3.527775876317849
5	1.450056728049697	3.292905593028366
6	1.14186125407458	3.111636589607164
7	0.9197547670490868	2.924114471704514
8	0.8108094261600958	2.9460021447560303
9	0.7064273916179956	2.887581978741526
>10	3.173002377325446	20.312437709719546
>50	0.04865268051649237	1.5828744693002696
>100	0.04253891041724263	3.3287357403312803
>500	0.003056688173182578	0.9196163207568891
>1k	0.0017830681010231708	1.3296201251049098
>5k	0.0	0.0
>10k+	2.5472401443188153E-4	6.721794875911029
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58102	6.679488697567532	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTT	2558	0.2940713243671086	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTG	2037	0.2341764221015638	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTC	1935	0.22245035678278152	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCT	1375	0.15807195895417292	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1302	0.14967977495151502	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCT	1217	0.13990805385252977	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1069	0.12289376299782608	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	952	0.10944327630863462	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.897685481636637E-4	0.0	0.0	0.06713747202126326	0.0
2	6.897685481636637E-4	0.0	0.0	0.2633766239738256	0.0
3	6.897685481636637E-4	0.0	0.0	0.6278043402536279	0.0
4	6.897685481636637E-4	0.0	0.0	0.9483167923003436	0.0
5	6.897685481636637E-4	0.0	0.0	1.7277552517252837	0.0
6	6.897685481636637E-4	0.0	0.0	2.787354703129365	0.0
7	6.897685481636637E-4	0.0	0.0	3.5119565629752936	0.0
8	6.897685481636637E-4	0.0	0.0	4.386583082046819	0.0
9	6.897685481636637E-4	0.0	0.0	4.935983730659177	0.0
10	6.897685481636637E-4	0.0	0.0	5.616555364847326	0.0
11	6.897685481636637E-4	0.0	0.0	6.335754037732639	0.0
12	6.897685481636637E-4	0.0	0.0	6.889293297633979	0.0
13	6.897685481636637E-4	0.0	0.0	7.204862408418855	0.0
14	6.897685481636637E-4	0.0	0.0	7.435704949204294	0.0
15	6.897685481636637E-4	0.0	0.0	7.6243566471270565	0.0
16	6.897685481636637E-4	0.0	0.0	7.884284428360064	0.0
17	6.897685481636637E-4	0.0	0.0	8.1835290168384	0.0
18	6.897685481636637E-4	0.0	0.0	8.51174388433961	0.0
19	6.897685481636637E-4	0.0	0.0	8.731320205505043	0.0
20	8.047299728576076E-4	0.0	0.0	8.970669891717835	0.0
21	8.047299728576076E-4	0.0	0.0	9.259912836247796	0.0
22	9.196913975515516E-4	0.0	0.0	9.55846765617797	0.0
23	9.196913975515516E-4	0.0	0.0	9.851504327722832	0.0
24	9.196913975515516E-4	0.0	0.0	10.08809493974297	0.0
25	0.0010346528222454955	0.0	0.0	10.311809872197385	0.0
26	0.0010346528222454955	0.0	0.0	10.534605113254248	0.0
27	0.0011496142469394396	0.0	0.0	10.778208372180714	0.0
28	0.0012645756716333834	0.0	0.0	10.999509114716556	0.0
29	0.0012645756716333834	0.0	0.0	11.247710830630782	0.0
30	0.0012645756716333834	0.0	0.0	11.53913804222993	0.0
31	0.0013795370963273273	0.0	0.0	11.79101852373436	0.0
32	0.0014944985210212714	0.0	0.0	12.054854993406963	0.0
33	0.0014944985210212714	0.0	0.0	12.323749765766097	0.0
34	0.0014944985210212714	0.0	0.0	12.60483044914279	0.0
35	0.0014944985210212714	0.0	0.0	12.870506301610495	0.0
36	0.0014944985210212714	0.0	0.0	13.11112056349492	0.0
37	0.0018393827951031032	0.0	0.0	13.375186956016908	0.0
38	0.002069305644490991	0.0	0.0	13.705011283463834	0.0
39	0.002069305644490991	0.0	0.0	14.239696869715367	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTACG	20	7.032338E-4	45.000004	1
TAACGCG	20	7.032338E-4	45.000004	1
GTATGCG	30	2.1648902E-6	45.000004	1
ATACGCG	20	7.032338E-4	45.000004	1
TGTAACG	20	7.032338E-4	45.000004	1
GCCGATA	25	3.8900213E-5	45.0	9
TATACGG	35	1.2114651E-7	45.0	2
CGTTTTT	32970	0.0	44.467697	1
GTTTTTT	36250	0.0	40.48759	2
TACGTAG	40	3.4569348E-7	39.375004	1
GTCACGG	35	6.246537E-6	38.571426	2
TCACGGG	100	0.0	38.25	3
ACGGGTG	95	0.0	37.894737	5
TACGAAT	95	0.0	37.894737	12
TATTACG	30	1.13969334E-4	37.500004	1
TACCGGT	30	1.13969334E-4	37.500004	40
GCGAGAC	135	0.0	36.666664	21
CGAACAG	25	0.0021068277	36.0	1
GTCGACG	25	0.0021068277	36.0	1
GGCGTAG	25	0.0021068277	36.0	1
>>END_MODULE