##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935678.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	539021
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40538494789628	33.0	31.0	34.0	30.0	34.0
2	32.60603761263476	34.0	31.0	34.0	31.0	34.0
3	32.44964110860245	34.0	31.0	34.0	30.0	34.0
4	36.06658924234863	37.0	35.0	37.0	35.0	37.0
5	36.098994287792124	37.0	35.0	37.0	35.0	37.0
6	36.10519998293202	37.0	36.0	37.0	35.0	37.0
7	36.2571643776402	37.0	37.0	37.0	35.0	37.0
8	36.06123138059556	37.0	37.0	37.0	35.0	37.0
9	37.87106068223687	39.0	38.0	39.0	35.0	39.0
10	37.6961732474245	39.0	38.0	39.0	35.0	39.0
11	37.634389012672976	39.0	37.0	39.0	35.0	39.0
12	37.50416959636081	39.0	37.0	39.0	35.0	39.0
13	37.44385840254832	39.0	37.0	39.0	35.0	39.0
14	38.63991755423258	41.0	38.0	41.0	34.0	41.0
15	38.81141551071294	41.0	38.0	41.0	34.0	41.0
16	38.923524315379176	41.0	38.0	41.0	35.0	41.0
17	38.929798653484745	41.0	38.0	41.0	35.0	41.0
18	38.27907818062747	39.0	38.0	40.0	35.0	41.0
19	37.579391155446636	37.0	37.0	40.0	35.0	41.0
20	36.74492644998989	35.0	35.0	40.0	34.0	41.0
21	36.70350320302919	35.0	35.0	40.0	33.0	41.0
22	36.717411752046765	35.0	35.0	40.0	34.0	41.0
23	36.748481042482574	35.0	35.0	40.0	34.0	41.0
24	36.59154281558603	35.0	35.0	40.0	34.0	41.0
25	36.49068218121372	35.0	35.0	40.0	33.0	41.0
26	36.341391151736204	35.0	35.0	40.0	33.0	41.0
27	36.26207698772404	35.0	35.0	40.0	33.0	41.0
28	36.11533131362229	35.0	35.0	40.0	33.0	41.0
29	35.84692618654932	35.0	35.0	40.0	33.0	41.0
30	35.32227686861922	35.0	35.0	40.0	31.0	41.0
31	34.64623641750507	35.0	35.0	40.0	24.0	41.0
32	33.617840492299926	35.0	34.0	40.0	18.0	41.0
33	32.47049558366	35.0	33.0	40.0	12.0	41.0
34	31.45256307268177	35.0	29.0	40.0	8.0	41.0
35	30.873426081729654	35.0	24.0	40.0	7.0	41.0
36	30.502683568914755	35.0	23.0	40.0	7.0	41.0
37	30.32762916472642	35.0	23.0	40.0	7.0	41.0
38	30.166653989362196	35.0	22.0	40.0	7.0	41.0
39	30.00217616753336	35.0	21.0	40.0	7.0	41.0
40	29.842601679711922	35.0	21.0	40.0	7.0	41.0
41	29.631392839982116	35.0	20.0	40.0	7.0	41.0
42	29.561377014995706	35.0	19.0	40.0	7.0	41.0
43	29.49604746382794	35.0	18.0	40.0	7.0	41.0
44	29.39815146348658	35.0	18.0	40.0	7.0	41.0
45	29.35856116923088	35.0	20.0	40.0	7.0	41.0
46	29.359705837063863	35.0	20.0	40.0	7.0	41.0
47	29.415640578010876	35.0	20.0	40.0	7.0	41.0
48	29.400758040966863	35.0	20.0	40.0	7.0	41.0
49	29.43414264008267	35.0	20.0	39.0	7.0	41.0
50	29.341393006951492	35.0	20.0	39.0	7.0	41.0
51	28.702660935288236	35.0	20.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	4.0
10	7.0
11	7.0
12	13.0
13	18.0
14	15.0
15	46.0
16	64.0
17	138.0
18	309.0
19	557.0
20	958.0
21	1499.0
22	2390.0
23	4272.0
24	8445.0
25	17374.0
26	29032.0
27	33659.0
28	30468.0
29	23045.0
30	17702.0
31	15117.0
32	15190.0
33	18508.0
34	31573.0
35	38824.0
36	38199.0
37	36547.0
38	62447.0
39	112500.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.709016902866495	13.615610523523202	15.018338803126408	45.6570337704839
2	59.030909741921	14.895523551030479	15.097927539001263	10.975639168047255
3	20.33649894902054	15.375282224625755	53.02149637954736	11.26672244680634
4	18.62283658707175	15.636311015711819	53.16490452134518	12.575947875871254
5	16.10345422534558	18.421731249802882	52.60685576257696	12.867958762274567
6	16.9603781670844	21.09992004022107	52.17588925106814	9.763812541626393
7	50.36427152188876	3.7493900979739196	42.446769235335914	3.4395691448014083
8	50.98855146645492	3.2059975399845273	42.31708968667269	3.4883613068878576
9	47.38479576862497	4.312633459549813	43.734474167054714	4.5680966047705
10	28.724112789668677	13.12267982137987	48.23151602627727	9.921691362674181
11	20.56802981702012	14.178482842041404	52.08238640052984	13.171100940408632
12	19.109459557234317	12.808591873043905	55.14330610495695	12.938642464764824
13	17.401733884208593	13.620248561744347	56.36366672170472	12.614350832342339
14	13.473315510898463	15.488264835692858	57.61779225670243	13.42062739670625
15	12.53940013468863	15.047651204684048	59.23925041881485	13.17369824181247
16	16.430157637643063	14.616128128588683	55.71768075826359	13.236033475504666
17	16.649073041681124	14.441737891473617	53.87879136434387	15.030397702501386
18	16.65389660143111	14.715567668050038	55.420660790581444	13.209874939937405
19	16.544438899412082	16.203821372451166	53.852076264190075	13.399663463946673
20	15.83129414252877	17.762016693227164	54.28415590487198	12.122533259372084
21	15.680279618048276	15.884724342836364	56.46013791670455	11.974858122410815
22	14.714825581934656	14.006318863272488	56.24919993840686	15.029655616386004
23	14.019119848762852	15.337250311212363	56.31005099986828	14.333578840156505
24	14.961569215299589	16.184898176508895	54.39064526242948	14.462887345762038
25	15.436133286087184	16.221631439220364	53.40942189636396	14.932813378328488
26	13.666072379369265	16.151504301316645	53.54114218184449	16.641281137469598
27	13.682398273907696	15.547075160336982	55.340886533177745	15.429640032577579
28	12.459996920342622	16.09547679960521	54.911033150841995	16.533493129210182
29	14.184234009435626	16.972437066459378	53.61943226701743	15.223896657087572
30	15.77637976999041	16.379695781797	52.60574263340389	15.2381818148087
31	15.868398448297933	17.07391734273804	51.73267831865549	15.32500589030854
32	17.831587266544346	18.468853718129722	48.17122152940238	15.528337485923554
33	17.484105443016134	19.722051645483198	45.88986328918539	16.903979622315273
34	15.551713198558126	21.06485647126921	46.10302752582923	17.28040280434343
35	17.504512811189173	23.58052840241846	41.7004161247892	17.214542661603165
36	18.991653386417227	23.741190046398934	40.00400726502307	17.26314930216077
37	18.151797425332223	24.653399403733804	40.31327165360904	16.881531517324927
38	19.514453054704735	24.34524814432091	38.653781578083226	17.48651722289113
39	18.426183766495182	23.43211117934181	38.97454830145764	19.167156752705367
40	19.822975357175324	22.718966422458493	40.15483626797472	17.303221952391464
41	17.97276914999601	23.32562182178431	38.37883867233373	20.32277035588595
42	17.59282105891978	23.34510158231312	39.706801775812075	19.355275582955024
43	18.023602048899765	22.743826307323832	39.53333914634124	19.699232497435165
44	18.36811552796644	22.592255218256803	39.47842477380288	19.561204479973878
45	18.668660404696663	22.067600334680836	38.906461900371234	20.35727736025127
46	18.31542741377423	22.456824502199357	40.21847015236883	19.00927793165758
47	16.201409592576173	22.04218388522896	42.87996200519089	18.876444517003975
48	16.600651922652364	22.451073334805137	41.862376419471595	19.0858983230709
49	18.14882908087069	20.887126846634917	42.46161095764358	18.502433114850813
50	16.513827847152523	21.385994237701315	42.416158183076355	19.684019732069807
51	16.44128892937381	21.910092556690742	40.8050892265793	20.84352928735615
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	129.0
1	305.5
2	482.0
3	33233.5
4	65985.0
5	42889.0
6	19793.0
7	19032.0
8	18271.0
9	18022.5
10	17774.0
11	17067.5
12	16361.0
13	15273.0
14	14185.0
15	12880.5
16	11576.0
17	10451.0
18	9326.0
19	8471.0
20	7616.0
21	6953.5
22	6291.0
23	5912.5
24	5534.0
25	5401.5
26	5110.0
27	4951.0
28	5120.0
29	5289.0
30	5770.5
31	6252.0
32	6553.5
33	6855.0
34	7734.5
35	8614.0
36	8971.5
37	9329.0
38	10023.0
39	10717.0
40	11844.5
41	12972.0
42	14795.5
43	16619.0
44	18528.0
45	20437.0
46	24000.5
47	27564.0
48	30906.5
49	34249.0
50	34174.0
51	34099.0
52	30165.5
53	26232.0
54	23992.0
55	21752.0
56	20544.5
57	19337.0
58	18771.0
59	18205.0
60	16240.0
61	14275.0
62	13287.0
63	12299.0
64	11256.5
65	10214.0
66	8486.0
67	6758.0
68	5900.5
69	5043.0
70	4278.5
71	3514.0
72	2931.5
73	2349.0
74	1754.5
75	886.0
76	612.0
77	542.5
78	473.0
79	320.0
80	167.0
81	110.0
82	53.0
83	34.5
84	16.0
85	13.5
86	11.0
87	9.5
88	8.0
89	4.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	539021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.6809277660687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.82506611115171	36.11338940600096
2	7.868954520224747	7.031843770252834
3	2.83324714516136	3.797763331091252
4	1.421201038979672	2.5400232385485
5	0.9189672660239142	2.0530155016298077
6	0.7329124570992946	1.9648325132703304
7	0.5676540095177766	1.7754315456768133
8	0.5080649773866656	1.8160651644066346
9	0.4583766239241881	1.8432623540889974
>10	3.7530884113740997	27.67281671267257
>50	0.07943796817878	2.526398593907837
>100	0.029266619853764244	2.276261968761478
>500	0.0033447565547159138	0.9426358550981059
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.180945693394892E-4	7.64626004459385
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40931	7.59358169718805	No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	767	0.14229501262474004	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	763	0.14155292650935677	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	696	0.12912298407668718	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	636	0.11799169234593827	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	617	0.1144667832978678	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10259340545173565	0.0
2	0.0	0.0	0.0	0.5025778216433126	0.0
3	0.0	0.0	0.0	0.7485793688928631	0.0
4	0.0	0.0	0.0	1.0405902552961759	0.0
5	0.0	0.0	0.0	1.8830435177850213	0.0
6	0.0	0.0	0.0	2.3791280859187305	0.0
7	0.0	0.0	0.0	2.8874570749562634	0.0
8	0.0	0.0	0.0	3.6328825778587475	0.0
9	0.0	0.0	0.0	3.923038248973602	0.0
10	0.0	0.0	0.0	4.558263963741672	0.0
11	0.0	0.0	0.0	5.507948669903399	0.0
12	0.0	0.0	0.0	6.250591349873196	0.0
13	0.0	0.0	0.0	6.53722211193998	0.0
14	0.0	0.0	0.0	6.679702646093566	0.0
15	0.0	0.0	0.0	6.903627131410465	0.0
16	0.0	0.0	0.0	7.350734015928879	0.0
17	0.0	0.0	0.0	7.8483027562933545	0.0
18	0.0	0.0	0.0	8.43900330413843	0.0
19	0.0	0.0	0.0	8.785743041551257	0.0
20	0.0	0.0	0.0	9.136564252598692	0.0
21	0.0	0.0	0.0	9.581630400299803	0.0
22	0.0	0.0	0.0	10.05118538980856	0.0
23	0.0	0.0	0.0	10.491613499288524	0.0
24	0.0	0.0	0.0	10.839095322816735	0.0
25	0.0	0.0	0.0	11.171920945566129	0.0
26	0.0	0.0	0.0	11.47821698969057	0.0
27	0.0	0.0	0.0	11.878386927410991	0.0
28	3.7104305769162984E-4	0.0	0.0	12.214551937679609	0.0
29	3.7104305769162984E-4	0.0	0.0	12.575391311284719	0.0
30	5.565645865374448E-4	0.0	0.0	12.973149469130146	0.0
31	5.565645865374448E-4	0.0	0.0	13.286866374408419	0.0
32	5.565645865374448E-4	0.0	0.0	13.607447576253987	0.0
33	7.420861153832597E-4	0.0	0.0	13.923576261407256	0.0
34	7.420861153832597E-4	0.0	0.0	14.275510601627765	0.0
35	7.420861153832597E-4	0.0	0.0	14.649522003780929	0.0
36	7.420861153832597E-4	0.0	0.0	14.956189090963061	0.0
37	7.420861153832597E-4	0.0	0.0	15.314802206222021	0.0
38	7.420861153832597E-4	0.0	0.0	15.722949569682815	0.0
39	7.420861153832597E-4	0.0	0.0	16.432198374460363	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGA	20	7.02995E-4	45.0	15
GCACGAA	20	7.02995E-4	45.0	34
ATCGTAG	20	7.02995E-4	45.0	15
ATATCGT	20	7.02995E-4	45.0	13
ATGACCG	20	7.02995E-4	45.0	18
GACGATA	25	3.88804E-5	45.0	9
TATCGTA	20	7.02995E-4	45.0	14
CGATATC	20	7.02995E-4	45.0	11
GCGTAAG	25	3.88804E-5	45.0	1
TACCGGT	20	7.02995E-4	45.0	40
CGTTTTT	20350	0.0	44.668304	1
CTACGAA	115	0.0	43.043476	11
TACGAAT	115	0.0	43.043476	12
GCTACGA	120	0.0	41.249996	10
GTTTTTT	22180	0.0	41.145176	2
ACGGGCC	55	6.002665E-11	40.909092	5
ATAGTAG	45	1.9250365E-8	40.000004	1
TCTACGG	40	3.4536606E-7	39.375	2
GACGGTC	40	3.4536606E-7	39.375	29
CGGTCTA	40	3.4536606E-7	39.375	31
>>END_MODULE