##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935677.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747035
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58338230471129	34.0	31.0	34.0	31.0	34.0
2	32.77916965068571	34.0	31.0	34.0	31.0	34.0
3	32.62066569839432	34.0	31.0	34.0	31.0	34.0
4	36.1874303078169	37.0	37.0	37.0	35.0	37.0
5	36.21943014718186	37.0	37.0	37.0	35.0	37.0
6	36.233678475573434	37.0	37.0	37.0	35.0	37.0
7	36.360518583466636	37.0	37.0	37.0	35.0	37.0
8	36.156458532732735	37.0	37.0	37.0	35.0	37.0
9	37.96688106982939	39.0	39.0	39.0	35.0	39.0
10	37.83414967170213	39.0	38.0	39.0	35.0	39.0
11	37.81564853052401	39.0	38.0	39.0	35.0	39.0
12	37.73832551353016	39.0	38.0	39.0	35.0	39.0
13	37.71933978996968	39.0	38.0	39.0	35.0	39.0
14	39.101779702423585	41.0	39.0	41.0	35.0	41.0
15	39.2232318432202	41.0	39.0	41.0	35.0	41.0
16	39.27844880092633	41.0	39.0	41.0	35.0	41.0
17	39.23039081167549	41.0	39.0	41.0	35.0	41.0
18	38.55319228684064	39.0	39.0	41.0	35.0	41.0
19	37.84563775458981	37.0	37.0	41.0	35.0	41.0
20	37.01235417349923	36.0	35.0	41.0	34.0	41.0
21	36.9694887120416	36.0	35.0	41.0	34.0	41.0
22	36.96782613933751	36.0	35.0	41.0	34.0	41.0
23	36.95697122624777	36.0	35.0	40.0	34.0	41.0
24	36.82151304825075	35.0	35.0	41.0	34.0	41.0
25	36.73103937566513	35.0	35.0	40.0	34.0	41.0
26	36.58469281894423	35.0	35.0	40.0	34.0	41.0
27	36.50951829566218	35.0	35.0	40.0	33.0	41.0
28	36.316438988802396	35.0	35.0	40.0	33.0	41.0
29	36.03535845040728	35.0	35.0	40.0	33.0	41.0
30	35.49426867549713	35.0	35.0	40.0	31.0	41.0
31	34.77363041892281	35.0	35.0	40.0	23.0	41.0
32	33.71232271580314	35.0	34.0	40.0	17.0	41.0
33	32.51911891678436	35.0	33.0	40.0	12.0	41.0
34	31.530225491442838	35.0	29.0	40.0	8.0	41.0
35	30.92535824961347	35.0	23.0	40.0	7.0	41.0
36	30.56350773390805	35.0	22.0	40.0	7.0	41.0
37	30.389319108207783	35.0	22.0	40.0	7.0	41.0
38	30.22704157101073	35.0	21.0	40.0	7.0	41.0
39	30.09362211944554	35.0	21.0	40.0	7.0	41.0
40	29.959155862844444	35.0	20.0	40.0	7.0	41.0
41	29.78744637132129	35.0	20.0	40.0	7.0	41.0
42	29.702971078998974	35.0	18.0	40.0	7.0	41.0
43	29.5940792600079	35.0	18.0	40.0	7.0	41.0
44	29.47492821621477	35.0	18.0	40.0	7.0	41.0
45	29.429835282148762	35.0	20.0	40.0	7.0	41.0
46	29.411425167495498	35.0	20.0	40.0	7.0	41.0
47	29.43358477179784	35.0	20.0	40.0	7.0	41.0
48	29.388023318853868	35.0	20.0	40.0	7.0	41.0
49	29.35038385082359	35.0	20.0	40.0	7.0	41.0
50	29.246537310835503	35.0	20.0	40.0	7.0	41.0
51	28.66369581077192	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	14.0
10	14.0
11	16.0
12	7.0
13	16.0
14	17.0
15	33.0
16	59.0
17	158.0
18	306.0
19	548.0
20	1101.0
21	1847.0
22	3130.0
23	5451.0
24	11245.0
25	23001.0
26	39836.0
27	49251.0
28	44781.0
29	33466.0
30	24643.0
31	19933.0
32	18653.0
33	21264.0
34	35341.0
35	47976.0
36	49846.0
37	46992.0
38	87463.0
39	180430.0
40	188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.97843474535999	12.717476423460747	14.792078015086307	46.51201081609295
2	59.474321818924146	14.414317936910587	15.445728781114672	10.665631463050593
3	21.209314155293928	14.841473291077392	53.18131011264532	10.767902440983354
4	18.154303345894103	15.707697765164951	53.32400757661957	12.813991312321377
5	15.963509072533416	18.362325727710214	53.256005408046484	12.418159791709893
6	16.359072868071777	21.706345753545683	52.36515022723166	9.569431151150882
7	50.59803088208719	3.660069474656475	42.65476182508182	3.0871378181745164
8	51.03107618786268	3.300648563989639	42.57484589075479	3.0934293573928935
9	47.45534011123977	4.704464984906999	43.756718226053664	4.083476677799568
10	25.47993065920606	16.46054067078517	48.46841178793497	9.591116882073798
11	19.770827337407216	16.809921891209918	51.72609047768846	11.693160293694405
12	18.450139551694363	14.366395148821676	55.006927386267044	12.176537913216917
13	16.91553943255671	14.34578031819125	55.71131205365211	13.027368195599939
14	13.203263568641363	17.317662492386567	55.71131205365211	13.767761885319965
15	12.591110188946969	16.267377030527353	58.45214748974278	12.689365290782895
16	14.686995923885762	16.10500177367861	56.84178117491149	12.366221127524145
17	14.834378576639647	15.220304269545604	54.97038291378583	14.974934240028915
18	15.908223844933639	15.789086187394167	54.951508296130704	13.351181671541493
19	16.564417999156667	16.866278019102186	53.98100490606197	12.588299075679185
20	16.967478096742454	16.760928202828516	53.95182287309163	12.319770827337408
21	16.630813817291024	15.80943329295147	55.19393334984305	12.365819539914462
22	14.727154684854124	15.849592053919832	54.920318325111936	14.502934936114103
23	14.587536059220788	16.328953797345505	55.1354354213658	13.948074722067908
24	14.635324984773138	15.928169362881256	55.05926763806247	14.377238014283133
25	15.122852342929047	16.710194301471816	53.75718674493163	14.409766610667504
26	13.919160414170689	16.857576954225706	54.1875547999759	15.0357078316277
27	13.749958167957324	17.025976025219702	54.89234105497065	14.331724751852324
28	13.460547363911997	17.182862918069432	55.21588680583909	14.140702912179485
29	14.349662331751526	17.31779635492313	54.362111547651715	13.97042976567363
30	15.02218771543502	16.432027950497634	54.10710341550262	14.438680918564724
31	15.577181792017777	18.082285301224175	51.631315801803126	14.709217104954922
32	16.132309731137095	19.446344548782854	49.557115797787255	14.864229922292798
33	17.029858038779977	20.8087974459028	46.83113910325486	15.330205412062353
34	16.15305842430408	21.90566707048532	45.229339990763485	16.711934514447112
35	16.44702055459249	23.836366435307582	42.518088175252835	17.198524834847095
36	17.972518021243985	25.33428821942747	40.52166230497902	16.171531454349527
37	18.042661990402056	25.474843882816735	40.14095725099895	16.34153687578226
38	18.32819078088711	25.620620185131887	38.90781556419712	17.143373469783878
39	18.962029891504415	24.106233309014975	38.553481429919614	18.378255369560996
40	18.786669968609235	24.51344314523416	39.995582536293476	16.704304349863126
41	16.8217017944273	25.848454222359056	39.16536708454089	18.164476898672753
42	17.644688669205593	25.81739811387686	39.24755868198947	17.29035453492808
43	18.609302107665638	24.73873379426667	38.95426586438386	17.69769823368383
44	17.6567362974961	24.890667773263637	39.06416700690061	18.38842892233965
45	17.478029811186893	23.95470091762769	39.13230303801027	19.434966233175153
46	17.288748184489346	24.394171625158126	39.928383542939756	18.38869664741277
47	16.763873178632863	23.917620995000235	41.46833816353986	17.850167662827044
48	16.73402183297971	23.65832926168118	41.200746952954006	18.406901952385095
49	17.38445989813061	22.38663516434973	41.92895915184697	18.29994578567269
50	15.893231240838782	22.498142657305216	42.1462180486858	19.4624080531702
51	16.045566807445436	22.5565067232459	40.6952820148989	20.702644454409764
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	168.0
1	437.0
2	706.0
3	47227.0
4	93748.0
5	62355.5
6	30963.0
7	29412.5
8	27862.0
9	26776.0
10	25690.0
11	24158.5
12	22627.0
13	21031.0
14	19435.0
15	17955.0
16	16475.0
17	15026.0
18	13577.0
19	12353.0
20	11129.0
21	10128.5
22	9128.0
23	8333.0
24	7538.0
25	7364.5
26	7080.5
27	6970.0
28	7595.0
29	8220.0
30	8763.5
31	9307.0
32	10012.0
33	10717.0
34	11682.5
35	12648.0
36	13750.0
37	14852.0
38	16093.0
39	17334.0
40	18873.5
41	20413.0
42	22474.0
43	24535.0
44	26739.5
45	28944.0
46	32434.0
47	35924.0
48	38674.0
49	41424.0
50	41361.5
51	41299.0
52	37429.5
53	33560.0
54	30426.0
55	27292.0
56	26434.0
57	25576.0
58	24810.0
59	24044.0
60	23329.5
61	22615.0
62	20629.5
63	18644.0
64	16030.5
65	13417.0
66	10803.0
67	8189.0
68	7013.0
69	5837.0
70	4773.5
71	3710.0
72	2995.0
73	2280.0
74	1884.0
75	1100.0
76	712.0
77	579.5
78	447.0
79	368.5
80	290.0
81	177.5
82	65.0
83	39.0
84	13.0
85	18.5
86	24.0
87	13.0
88	2.0
89	3.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	747035.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.30589774642315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21577257556379	32.331687270810086
2	7.359249931903227	5.932423504913259
3	2.6135462205246154	3.160239801600475
4	1.4044043708594416	2.2642311586596153
5	0.9555832609599823	1.9257820602223314
6	0.7810307518898728	1.8888087373491118
7	0.6736330935463858	1.9005970610961125
8	0.6006133172155214	1.9366607159063047
9	0.5162484130956323	1.8727070174987197
>10	4.736843838807724	32.44416898843625
>50	0.08577752348042576	2.4497144967891544
>100	0.05361094938328977	3.5083896949033355
>500	0.0026805474691644487	0.7395466151855602
>1k	6.701368672911122E-4	0.2827996004745367
>5k	0.0	0.0
>10k+	3.350684336455561E-4	7.362243276155154
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54514	7.2973823180975455	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1089	0.14577630231515257	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1005	0.13453184924401132	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	896	0.11994083275883995	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	796	0.10655457910271943	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	777	0.10401119090805652	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	748	0.10012917734778157	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.677250731224106E-4	0.0	0.0	0.07349053257210171	0.0
2	2.677250731224106E-4	0.0	0.0	0.3535309590581432	0.0
3	2.677250731224106E-4	0.0	0.0	0.5270168064414653	0.0
4	2.677250731224106E-4	0.0	0.0	0.7254010856251715	0.0
5	2.677250731224106E-4	0.0	0.0	1.3044904187889457	0.0
6	2.677250731224106E-4	0.0	0.0	1.653603914140569	0.0
7	2.677250731224106E-4	0.0	0.0	1.9669761122303506	0.0
8	2.677250731224106E-4	0.0	0.0	2.4867643416975107	0.0
9	2.677250731224106E-4	0.0	0.0	2.6801957070284526	0.0
10	2.677250731224106E-4	0.0	0.0	3.150722523041089	0.0
11	2.677250731224106E-4	0.0	0.0	3.770639929856031	0.0
12	2.677250731224106E-4	0.0	0.0	4.305554625954607	0.0
13	2.677250731224106E-4	0.0	0.0	4.516655846111628	0.0
14	2.677250731224106E-4	0.0	0.0	4.605942157997952	0.0
15	2.677250731224106E-4	0.0	0.0	4.770860803041357	0.0
16	2.677250731224106E-4	0.0	0.0	5.098556292543187	0.0
17	2.677250731224106E-4	0.0	0.0	5.464001017355278	0.0
18	2.677250731224106E-4	0.0	0.0	5.926763806247364	0.0
19	2.677250731224106E-4	0.0	0.0	6.17173224815437	0.0
20	2.677250731224106E-4	0.0	0.0	6.458465801468472	0.0
21	2.677250731224106E-4	0.0	0.0	6.774515250289478	0.0
22	2.677250731224106E-4	0.0	0.0	7.128180071884183	0.0
23	2.677250731224106E-4	0.0	0.0	7.470198852798061	0.0
24	2.677250731224106E-4	0.0	0.0	7.7475620285528795	0.0
25	4.015876096836159E-4	0.0	0.0	7.9865066563146305	0.0
26	4.015876096836159E-4	0.0	0.0	8.212065030420261	0.0
27	4.015876096836159E-4	0.0	0.0	8.47189221388556	0.0
28	4.015876096836159E-4	0.0	0.0	8.7184670062313	0.0
29	4.015876096836159E-4	0.0	0.0	8.970128574966367	0.0
30	4.015876096836159E-4	0.0	0.0	9.273996532960304	0.0
31	4.015876096836159E-4	0.0	0.0	9.512807298185493	0.0
32	4.015876096836159E-4	0.0	0.0	9.770224955992692	0.0
33	5.354501462448212E-4	0.0	0.0	10.010508209120054	0.0
34	5.354501462448212E-4	0.0	0.0	10.256145963709866	0.0
35	5.354501462448212E-4	0.0	0.0	10.531233476343143	0.0
36	5.354501462448212E-4	0.0	0.0	10.793871773076228	0.0
37	5.354501462448212E-4	0.0	0.0	11.06400637185674	0.0
38	6.693126828060265E-4	0.0	0.0	11.358905539901075	0.0
39	6.693126828060265E-4	0.0	0.0	11.757682036316906	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGTA	35	1.2111559E-7	45.000004	28
ATGCGAG	30	2.1644773E-6	45.000004	1
CGGTCTA	55	1.8189894E-12	45.000004	31
TCGAGTA	20	7.0316985E-4	45.000004	43
GCGTACG	20	7.0316985E-4	45.000004	1
ATCGAGT	20	7.0316985E-4	45.000004	42
TACGTAG	20	7.0316985E-4	45.000004	1
GTACGAG	25	3.8894897E-5	45.0	1
CGTTTTT	28670	0.0	44.591908	1
TCACGAC	60	3.6379788E-12	41.250004	25
GTTTTTT	31505	0.0	40.75067	2
TACGGGA	150	0.0	40.500004	4
GTGCTAG	650	0.0	39.115387	1
TAGCCGT	35	6.245351E-6	38.57143	44
TGTTGCG	35	6.245351E-6	38.57143	1
CGACGGT	65	9.094947E-12	38.076927	28
ACGGTCT	65	9.094947E-12	38.076927	30
CGTTAGG	95	0.0	37.894737	2
TACCACG	30	1.139539E-4	37.500004	34
TAGCGAG	30	1.139539E-4	37.500004	1
>>END_MODULE