SRR2935675.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935675.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	532426
Sequences flagged as poor quality	0
Sequence length	51
%GC	33

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40956	7.692336587619689	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1180	0.2216270430069155	RNA PCR Primer, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1123	0.21092132991251367	RNA PCR Primer, Index 38 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	769	0.14443321701043899	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	769	0.14443321701043899	RNA PCR Primer, Index 38 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	691	0.12978329382862594	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	620	0.11644810734261661	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	599	0.11250389725520542	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	533	0.1001078084090559	RNA PCR Primer, Index 27 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGACCG	25	3.887974E-5	45.000004	9
TGCGTAG	25	3.887974E-5	45.000004	1
CCACACG	25	3.887974E-5	45.000004	27
TTGTGCG	20	7.029873E-4	45.0	1
CGCGCCC	20	7.029873E-4	45.0	22
GTTAGCG	20	7.029873E-4	45.0	1
GTTACCG	20	7.029873E-4	45.0	38
TATTCGG	20	7.029873E-4	45.0	2
TATCGTG	20	7.029873E-4	45.0	1
TAATCCG	20	7.029873E-4	45.0	1
CGTTTTT	19620	0.0	44.587154	1
GCTACGA	80	0.0	42.1875	10
AGCTACG	80	0.0	42.1875	9
CGAATAT	75	0.0	42.000004	14
GACCGAT	110	0.0	40.909092	9
GTTTTTT	21550	0.0	40.75058	2
GGAACGT	45	1.9250365E-8	40.0	8
ATTGGGC	45	1.9250365E-8	40.0	4
TAGGGCG	120	0.0	39.375004	5
ATAACGG	40	3.4535697E-7	39.375	2