##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935675.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	532426
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39617899952294	33.0	31.0	34.0	30.0	34.0
2	32.59883814839996	34.0	31.0	34.0	31.0	34.0
3	32.450387471686206	34.0	31.0	34.0	30.0	34.0
4	36.05650174859981	37.0	35.0	37.0	35.0	37.0
5	36.1046417718143	37.0	35.0	37.0	35.0	37.0
6	36.12540146424104	37.0	36.0	37.0	35.0	37.0
7	36.2793345929763	37.0	37.0	37.0	35.0	37.0
8	36.08386329743476	37.0	37.0	37.0	35.0	37.0
9	37.91806748731279	39.0	39.0	39.0	35.0	39.0
10	37.725513029040656	39.0	38.0	39.0	35.0	39.0
11	37.66101016854925	39.0	37.0	39.0	35.0	39.0
12	37.67563943158298	39.0	38.0	39.0	35.0	39.0
13	37.701196034754126	39.0	38.0	39.0	35.0	39.0
14	39.120820545953805	41.0	39.0	41.0	35.0	41.0
15	39.22167399788891	41.0	39.0	41.0	35.0	41.0
16	39.25646756544572	41.0	39.0	41.0	35.0	41.0
17	39.22096404007318	41.0	39.0	41.0	35.0	41.0
18	38.56896545247602	39.0	39.0	41.0	35.0	41.0
19	37.88696457348063	37.0	37.0	41.0	35.0	41.0
20	37.081568142802944	37.0	35.0	41.0	34.0	41.0
21	37.044533888277435	37.0	35.0	41.0	34.0	41.0
22	37.03006802823303	37.0	35.0	41.0	34.0	41.0
23	37.05125219279299	37.0	35.0	40.0	34.0	41.0
24	36.89998797955021	37.0	35.0	41.0	34.0	41.0
25	36.81143107211143	36.0	35.0	40.0	34.0	41.0
26	36.658472726726345	36.0	35.0	40.0	33.0	41.0
27	36.57186538598791	36.0	35.0	40.0	33.0	41.0
28	36.449311265790925	36.0	35.0	40.0	33.0	41.0
29	36.21520924973611	36.0	35.0	40.0	33.0	41.0
30	35.68781201519084	36.0	35.0	40.0	31.0	41.0
31	35.05372577597638	36.0	35.0	40.0	25.0	41.0
32	34.049199325352255	36.0	34.0	40.0	18.0	41.0
33	32.9247069076266	36.0	33.0	40.0	12.0	41.0
34	32.00729303227115	36.0	31.0	40.0	8.0	41.0
35	31.463330115358755	36.0	27.0	40.0	7.0	41.0
36	31.099027846123217	35.0	24.0	40.0	7.0	41.0
37	30.896759361864373	35.0	23.0	40.0	7.0	41.0
38	30.74637977859834	35.0	23.0	40.0	7.0	41.0
39	30.620420490359223	35.0	23.0	40.0	7.0	41.0
40	30.501513825395453	35.0	22.0	40.0	7.0	41.0
41	30.344754012764216	35.0	21.0	40.0	7.0	41.0
42	30.215340723405696	35.0	21.0	40.0	7.0	41.0
43	30.0770247884213	35.0	20.0	40.0	7.0	41.0
44	29.937170611502818	35.0	20.0	40.0	7.0	41.0
45	29.910490847554403	35.0	20.0	40.0	7.0	41.0
46	29.89583153339619	35.0	20.0	40.0	7.0	41.0
47	29.934597483969604	35.0	20.0	40.0	7.0	41.0
48	29.908248282390417	35.0	20.0	40.0	7.0	41.0
49	29.909333879262096	35.0	20.0	40.0	7.0	41.0
50	29.819655689241323	35.0	20.0	40.0	7.0	41.0
51	29.22862144222859	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	6.0
11	9.0
12	8.0
13	6.0
14	23.0
15	21.0
16	41.0
17	131.0
18	265.0
19	478.0
20	792.0
21	1355.0
22	2017.0
23	3694.0
24	7223.0
25	15122.0
26	26429.0
27	33698.0
28	30026.0
29	23351.0
30	17777.0
31	14860.0
32	13776.0
33	14855.0
34	21947.0
35	33422.0
36	36564.0
37	33710.0
38	65114.0
39	135566.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.227780010743277	13.879675297600041	15.946441383403515	45.946103308253164
2	57.62077734746237	15.374906559784835	16.046173552756628	10.95814253999617
3	20.141953999241206	14.826473538106704	54.163583296082464	10.867989166569627
4	18.25568247981879	16.683257391637525	53.36553812172959	11.695522006814093
5	16.13520000901534	18.766551595902527	52.664783462866204	12.433464932215932
6	16.72232385345569	21.74536930953785	51.83067693914272	9.701629897863741
7	51.392493980384124	3.7960204798413297	41.81257113664622	2.998914403128322
8	51.915007907202124	2.9872695923940604	41.731433100562334	3.36628939984148
9	48.189044111294336	4.984542452847907	42.961463189250715	3.86495024660704
10	23.299763723033813	21.298546652492554	47.32037879442401	8.081310830049622
11	15.823983051165797	15.793180648578394	55.64810884517285	12.73472745508296
12	16.10571234312374	14.339081863019462	57.050745080067465	12.504460713789333
13	14.8204633132116	15.407587157651955	56.974302532182875	12.797646996953569
14	11.949078369576242	17.918358607581148	56.015483841886	14.117079180956601
15	11.626780059576355	17.630994729784042	58.21954600263699	12.522679208002616
16	13.185494322215668	17.110546817773738	56.489352510959264	13.214606349051325
17	12.679508513859203	16.87821406167242	54.99975583461364	15.44252158985474
18	12.899069542058427	17.261365898735225	56.270167121815994	13.569397437390359
19	13.893198303614025	17.51097805140996	55.020603802218524	13.575219842757491
20	15.124167489942264	17.464586628000887	55.4655482639841	11.945697618072746
21	14.708710694068284	17.59098917032602	55.672713203337175	12.027586932268521
22	13.204276275012866	15.973111756375532	55.32261760319744	15.49999436541416
23	12.598182658247342	17.111298095885623	55.52170630284772	14.768812943019311
24	13.669693065327387	16.16036782576358	55.81770987893153	14.352229229977498
25	12.921795704943035	17.640761345238587	54.67464023169417	14.76280271812421
26	12.796144440729792	18.803739862440978	54.21748750061042	14.182628196218817
27	13.696363438299407	17.980339051811896	55.126158376938776	13.197139132949932
28	12.36810373648169	17.64752284824558	55.662195309770745	14.322178105501985
29	13.902777099540595	16.332222693857926	54.46484581894949	15.300154387651993
30	14.17417631746008	17.594182102301538	53.867767539526625	14.36387404071176
31	14.856149023526275	18.98122931637448	51.40601698639811	14.756604673701135
32	14.900474432127659	19.909996882195834	49.75301732071687	15.436511364959637
33	15.068572909662564	21.52205189077919	47.83650685729096	15.572868342267283
34	15.321377994312824	22.235953916600618	45.53252470765891	16.910143381427652
35	15.539436466288272	23.032120895673767	43.85999181106858	17.568450826969382
36	18.05640596064054	24.581444181914485	41.67583852028263	15.686311337162348
37	16.99372307137518	25.611258653784752	41.60916258785258	15.785855686987487
38	16.260287814644663	24.94393587090037	40.615597284880906	18.180179029574063
39	17.247279434137326	24.045782888138444	40.98297228159406	17.72396539613017
40	16.701475885850805	24.556652004222183	42.0022688598979	16.739603250029113
41	16.074534301480394	25.810910812018946	40.19243988836007	17.922114998140586
42	17.942211687633584	25.34605748028834	39.37523712215407	17.33649370992401
43	18.270708042056548	24.60792673535853	39.28113953864011	17.84022568394481
44	16.740166708613028	24.664084774222143	39.62146852332531	18.97427999383952
45	16.85792955265145	23.71691089465954	40.04162080739859	19.383538745290423
46	17.156374782598895	24.238110084781734	41.27784894051004	17.327666192109326
47	15.910192214504926	23.537731064974288	43.34311247008974	17.20896425043105
48	15.862861693455994	22.574780345062038	43.19511068204783	18.36724727943414
49	16.238688568927888	21.815613812999366	43.96836367870841	17.977333939364343
50	15.504126395029544	21.623474435884045	43.78317362412805	19.08922554495836
51	15.001521338176573	21.778425546460916	42.22915484968803	20.990898265674478
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	299.0
2	454.0
3	33463.5
4	66473.0
5	44008.0
6	21543.0
7	20302.0
8	19061.0
9	18269.0
10	17477.0
11	16730.0
12	15983.0
13	15331.0
14	14679.0
15	13264.0
16	11849.0
17	10527.0
18	9205.0
19	8404.5
20	7604.0
21	6917.0
22	6230.0
23	5786.5
24	5343.0
25	5218.5
26	5510.5
27	5927.0
28	6175.5
29	6424.0
30	6962.0
31	7500.0
32	7927.5
33	8355.0
34	9816.0
35	11277.0
36	12002.0
37	12727.0
38	13531.0
39	14335.0
40	15616.0
41	16897.0
42	19141.5
43	21386.0
44	23606.0
45	25826.0
46	28820.5
47	31815.0
48	35000.0
49	38185.0
50	36353.5
51	34522.0
52	28961.0
53	23400.0
54	20239.0
55	17078.0
56	15193.5
57	13309.0
58	12297.0
59	11285.0
60	10527.0
61	9769.0
62	8439.5
63	7110.0
64	6200.5
65	5291.0
66	4337.0
67	3383.0
68	2701.5
69	2020.0
70	1704.0
71	1388.0
72	1189.0
73	990.0
74	760.0
75	404.0
76	278.0
77	225.5
78	173.0
79	127.5
80	82.0
81	50.0
82	18.0
83	11.0
84	4.0
85	2.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	532426.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.39859920666007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07552656724019	33.526915579800175
2	8.618024292789467	7.307843158864815
3	3.0290739537345885	3.852854775951781
4	1.5376682267338186	2.6077991543241152
5	1.0120172342386529	2.1454056552358636
6	0.7990953331849081	2.032831365577165
7	0.6426114870880787	1.9072078820650282
8	0.5490319344921499	1.8622547953751931
9	0.5270560698151388	2.0111795157176564
>10	4.067271165602311	28.065981244573262
>50	0.10104377469705	3.0214381020480534
>100	0.03755609324980157	2.7033520781065166
>500	0.0026825780892715404	0.7546486657977731
>1k	8.941926964238468E-4	0.43656264188200744
>5k	0.0	0.0
>10k+	4.470963482119234E-4	7.763725384680632
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40956	7.692336587619689	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1180	0.2216270430069155	RNA PCR Primer, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1123	0.21092132991251367	RNA PCR Primer, Index 38 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	769	0.14443321701043899	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	769	0.14443321701043899	RNA PCR Primer, Index 38 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	691	0.12978329382862594	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	620	0.11644810734261661	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	599	0.11250389725520542	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	533	0.1001078084090559	RNA PCR Primer, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.878195279719623E-4	0.0	0.0	0.1510069004894577	0.0
2	1.878195279719623E-4	0.0	0.0	0.7755068309962323	0.0
3	1.878195279719623E-4	0.0	0.0	1.1098255907863253	0.0
4	1.878195279719623E-4	0.0	0.0	1.5179574250693992	0.0
5	1.878195279719623E-4	0.0	0.0	2.560731444369734	0.0
6	1.878195279719623E-4	0.0	0.0	3.0757325900688546	0.0
7	1.878195279719623E-4	0.0	0.0	3.6298001975861434	0.0
8	1.878195279719623E-4	0.0	0.0	4.475175892987946	0.0
9	1.878195279719623E-4	0.0	0.0	4.755026989666169	0.0
10	1.878195279719623E-4	0.0	0.0	5.4285478169736265	0.0
11	1.878195279719623E-4	0.0	0.0	6.54588618887883	0.0
12	1.878195279719623E-4	0.0	0.0	7.491745331745633	0.0
13	1.878195279719623E-4	0.0	0.0	7.835830706990267	0.0
14	1.878195279719623E-4	0.0	0.0	7.97012166949022	0.0
15	1.878195279719623E-4	0.0	0.0	8.21804344641321	0.0
16	1.878195279719623E-4	0.0	0.0	8.727034367217229	0.0
17	1.878195279719623E-4	0.0	0.0	9.424596094105096	0.0
18	1.878195279719623E-4	0.0	0.0	10.135493007478974	0.0
19	1.878195279719623E-4	0.0	0.0	10.563721531255048	0.0
20	1.878195279719623E-4	0.0	0.0	10.989696220695459	0.0
21	1.878195279719623E-4	0.0	0.0	11.505073005450523	0.0
22	1.878195279719623E-4	0.0	0.0	12.095953240450315	0.0
23	1.878195279719623E-4	0.0	0.0	12.656594531446624	0.0
24	1.878195279719623E-4	0.0	0.0	13.08407177711081	0.0
25	1.878195279719623E-4	0.0	0.0	13.455015344855436	0.0
26	1.878195279719623E-4	0.0	0.0	13.791775758509164	0.0
27	1.878195279719623E-4	0.0	0.0	14.140556621953097	0.0
28	1.878195279719623E-4	0.0	0.0	14.508870716306117	0.0
29	1.878195279719623E-4	0.0	0.0	14.879250825466826	0.0
30	1.878195279719623E-4	0.0	0.0	15.305413334435208	0.0
31	1.878195279719623E-4	0.0	0.0	15.698707426008497	0.0
32	1.878195279719623E-4	0.0	0.0	16.066270242249626	0.0
33	1.878195279719623E-4	0.0	0.0	16.432706141322925	0.0
34	1.878195279719623E-4	0.0	0.0	16.775476779871756	0.0
35	3.756390559439246E-4	0.0	0.0	17.162197187966026	0.0
36	5.634585839158869E-4	0.0	0.0	17.530511282319047	0.0
37	5.634585839158869E-4	0.0	0.0	17.883799814434305	0.0
38	5.634585839158869E-4	0.0	0.0	18.23445887315796	0.0
39	5.634585839158869E-4	0.0	0.0	18.61084920721377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGACCG	25	3.887974E-5	45.000004	9
TGCGTAG	25	3.887974E-5	45.000004	1
CCACACG	25	3.887974E-5	45.000004	27
TTGTGCG	20	7.029873E-4	45.0	1
CGCGCCC	20	7.029873E-4	45.0	22
GTTAGCG	20	7.029873E-4	45.0	1
GTTACCG	20	7.029873E-4	45.0	38
TATTCGG	20	7.029873E-4	45.0	2
TATCGTG	20	7.029873E-4	45.0	1
TAATCCG	20	7.029873E-4	45.0	1
CGTTTTT	19620	0.0	44.587154	1
GCTACGA	80	0.0	42.1875	10
AGCTACG	80	0.0	42.1875	9
CGAATAT	75	0.0	42.000004	14
GACCGAT	110	0.0	40.909092	9
GTTTTTT	21550	0.0	40.75058	2
GGAACGT	45	1.9250365E-8	40.0	8
ATTGGGC	45	1.9250365E-8	40.0	4
TAGGGCG	120	0.0	39.375004	5
ATAACGG	40	3.4535697E-7	39.375	2
>>END_MODULE